Help! GFP transfection - (Jan/25/2007 )
QUOTE (scolix @ Jan 28 2007, 06:13 PM)
QUOTE (Kathy @ Jan 28 2007, 06:26 PM)
we use new born or postnatal mice here to prepare SCG neurons for transfection. embryonic mice will mean we have to kill the mother and this is not an option here ![rolleyes.gif]()
However, I do think that PEI is not very effecient for SCG.![dry.gif]()
i cant get repeatable results and dont like it. but to transfect with virus i need to clone my gene again into the virus genome right? ![unsure.gif]()
However, I do think that PEI is not very effecient for SCG.
Kathy,
You will need to clone your gene into a viral vector and you will have to make the virus, it might not be the best option for you if you wish to complete your project within a short time (within a month).
i see. thank you Scolix!
-Kathy-
Kathy ,
I read a paper where they have transfected hippocampal neurons 7 DIV using Qiagen's Effectene. May b its helpful.
-scolix-
QUOTE (scolix @ Jan 30 2007, 11:48 AM)
Kathy ,
I read a paper where they have transfected hippocampal neurons 7 DIV using Qiagen's Effectene. May b its helpful.
I read a paper where they have transfected hippocampal neurons 7 DIV using Qiagen's Effectene. May b its helpful.
i think that ordering something new is not an option here either...
-Kathy-