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Help with Cloning into LARGE vector - (Jan/05/2007 )

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loriv
can i keep the ligation mix in refrigerator for 4c?? or i need to keep it in a water bath of 4c???

-ligation doesn't works-

QUOTE (ligation doesn @ Feb 1 2007, 10:37 PM)
loriv
can i keep the ligation mix in refrigerator for 4c?? or i need to keep it in a water bath of 4c???



for the 4 C part of the ligation I kept it in the fridge. For the 16 degree part I used a pcr machine.

-LoriV-

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