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TBS/casein 1% freeze - (Oct/11/2006 )

Dear member list,
I'm doing WB and I would like to know if I can freeze the TBS/casein 1% with the primary antibody in order to reuse it?
Many thanks for the reply

-amicia-

I often do so, It always worked.
I re-use them twice.

-Missele-

QUOTE (amicia @ Oct 11 2006, 03:28 PM)
Dear member list,
I'm doing WB and I would like to know if I can freeze the TBS/casein 1% with the primary antibody in order to reuse it?
Many thanks for the reply


prefer shock freezing with liquid N2; if you re-use it in a manageable time (~2 weeks) do not freeze, only cool at 4°C

-The Bearer-