TBS/casein 1% freeze - (Oct/11/2006 )
Dear member list,
I'm doing WB and I would like to know if I can freeze the TBS/casein 1% with the primary antibody in order to reuse it?
Many thanks for the reply
-amicia-
I often do so, It always worked.
I re-use them twice.
-Missele-
QUOTE (amicia @ Oct 11 2006, 03:28 PM)
Dear member list,
I'm doing WB and I would like to know if I can freeze the TBS/casein 1% with the primary antibody in order to reuse it?
Many thanks for the reply
I'm doing WB and I would like to know if I can freeze the TBS/casein 1% with the primary antibody in order to reuse it?
Many thanks for the reply
prefer shock freezing with liquid N2; if you re-use it in a manageable time (~2 weeks) do not freeze, only cool at 4°C
-The Bearer-