Mutation Scanning using HRMA - (Sep/21/2006 )

Hi, I am very interested in this 'High resolution melting analysis' for mutation scanning.

1) Is this high resolution melting analysis a new technology in mutation scanning? (Using LightScanner, HR-1 or Rotor Gene 6000)

2) Is anyone from UK or Malaysia working on this high resolution melting analysis?

Thanks.

Rachel

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On our real-time pcr machine we have software for a high resolution melting curve, I assume this is the same. I don't exactly know how new this is, the theory behind it must be quite old, but this software is quite new to us. People are working a lot with it on our machine, it is a very fast method to scan for mutations.

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to aspergillie

How about your HRM results for mutation scanning? I`m doing this work.Among 12 mutations which were confirmed by DNA sequencing, 9 mutations were confirmed by HRM. Some papers reported 100% sensitivity of the HRM. Would you say something about this? Thank you.

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Hi Leydig,

I am not able to say anything about it, I have never worked myself with HRM. I only know there are some people from an other group that have worked with this on our machine.
I can speculate that it does matter which mutation you have to be able to see it, an C to A mutation for instance is I think easier to recognize than a C to G mutation. Maybe other peolpe here on the forum know more about this.

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