antibody- left in room temp for a few days - still working? (Sep/21/2006 )

It recalls me this story : the damned room number 15 in the recovery room.
It doesn't matter how resistant, or how sick was the patient, but on each monday at 7:00 PM, the patient in the room 15 was dying
This was quite strange, and very regular




....


guess what : to plug the vacuum cleaner, the cleaning lady was unpluging the respirator machine !

sorry, I thought you would need to read something funny, but I'm not really good in telling a joke.

In the body (a much harsher environment then being held sterily at room temperature with stabilizers and preservatives):

IgG has a half-life of 20 days.
IgM has a half-life of 10 days.
IgA has a half-life of 6 days.
IgE has a half-life of 2 days.
IgD has a half-life of 2 days.

i will check those antibodies and tell you the result.
thanks everybody for help.
my PI is on holiday now, tomorrow she will be back and get informed on this fridge thing. it won't be a nice day tomorrow i guess. (we also have PKA inhibitor&activator, in vitro transcription kit and RNA polymerase there)

Hello
Myself Arun from Mumbai. I would kindly request you to brief me the dot blot for checking the antibody functioning? Plz do write the dot blot in short, if possible?...
thanks

It's like a western-blot, but instead to migrate the sample on acrylamide gel, and transfer to nitrocellulose membrane or PVDF membrane, you put a little drop of your sample directly on the dry membrane (1-2 µL)
you let dry. then you hydrate your membrane, block, incubate with primary antibody, wash, incubate with enzyme linked secondary antibody, wash, add substrate.

HI Missele
Thanks for giving me hint for dot blot. I have gathered more information from net and i will be doing it soon.
all the best for ur lab work...
bye

That is so bad I never got to know if the antibodies worked!!!!

hi,
I have just realized that I did not inform you on the antibody's activity. It has been working nicely since that day
Thank you all

It of course depends, but I can confidently bet that it will work with no problems! Most antibodies are nasty creatures, they are hard to kill. So if it worked well before, it will keep on working.

Once our lab's +4C fridge broke down, but we did not know about it until one newcomer asked why solutions taken from it are not cold (fridge light was still on). We had a bunch of antibodies to be kept at +4C and were using them continuously. They all worked fine, even though God knows how long they stayed in that broken fridge.

No worries.