How to prepare DNA samples to be sent on filter paper? - (Feb/15/2006 )
Hi,
I am going to do a research in Australia. I planned to sending my plasmid constructs before I visit Australia. I don't think it's a good idea to carry the plasmid solution into Austrlia as I know cuz they require biological import permit.
My friend told me that I could just spot the plasmids on filter paper and send them to Australia. But I don't know how to prepare the samples and filter papers.
Could I just purified the plasmids from the cells using boiling prep or alkaline lysis prep and spot on the sterile filter paper? Please give me some advice on this. Thank you.
Please check this thread for answer
http://www.protocol-online.org/forums/inde...showtopic=13565
When you send it to Australia by mail, it is better to state the filter paper as "sample" rather than "DNA sample".
My friend (from Australia) told me that someone from US sent some DNA on filter paper and labeled it as DNA sample and the mail got stuck in the custom office. She eventually got the mail after a lot of explanation!!!!
I am going to do a research in Australia. I planned to sending my plasmid constructs before I visit Australia. I don't think it's a good idea to carry the plasmid solution into Austrlia as I know cuz they require biological import permit.
My friend told me that I could just spot the plasmids on filter paper and send them to Australia. But I don't know how to prepare the samples and filter papers.
Could I just purified the plasmids from the cells using boiling prep or alkaline lysis prep and spot on the sterile filter paper? Please give me some advice on this. Thank you.
hi oddie,
please check this publication:
Biotechniques. 1990 May;8(5):509. Related Articles, Links
Improved method for plasmid shipment.
Rosman GJ, Miller AD.
Fred Hutchinson Cancer Ctr. Molecular Medicine AB133, Seattle, WA 98104-2092
i use this method with little modification (more DNA in less volume) for sending plasmids to other labs and up to now there were no problems for solving the DNA and retransforming it into e. coli for amplification.
hope, it helps
flausch
My friend (from Australia) told me that someone from US sent some DNA on filter paper and labeled it as DNA sample and the mail got stuck in the custom office. She eventually got the mail after a lot of explanation!!!!
Thank you pcrman and Minnie Mouse. I just don't want to test the by transforming the spotted DNA in the competent cells cuz I have no time to test that so far. T ^ T You are right Minnie Mouse. Australian officers are very strict about biological sample import. I planned to carry the samples with me but I was told that they were very strict about this issue. That's why I will send the samples before I visit Australia. Also, I have a sample import permit with me. It should be no problem with that I think.