ELISA on cow fecal and milk specimens - (Jan/13/2006 )

I need to do an elisa using fecal and milk specimens (from cows). Has anyone had exprience using these kinds of samples? If so, what protocol do you use for sample prep?
Thanks, Cleo.

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hi,

i have never done such an experiment, but in my case, i would dilute filtered milk samples in coating buffer exactly as with sera; in the case of fecal samples, try to wade solid in some coating buffer, crush it, filter it and centrifuge it to recover supernatant...

Sébastien

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Thanks,
I'll try your suggestions. I already have antibody coated plates that have been blocked and I want to look for the presence of bacteria or bacterial antigens in the milk and feces. It will be a sandwich ELISA with an HRP conjugated antibody that is the same that was coated on the plates. Would you still make the same suggestions?
Thanks
Cleo

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it depends if you are looking for antigens of for whole bacterial cells;
in the last case don't filter your samples and only dilute it...

Sébastien_

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