what is the role of sodium azide in binding buffer - antibody purification (Dec/28/2005 )
just a contradiction :
sodium azide is forbidden in many countries, but my lab bought precasted gels from Bi...d and they were stored in N&N3, which actually is sodium azide if i'm not wrong?... 
-fred_33-
Apparently (according to our tecnition) if it contacts lead then Sodium Azide forms an explosive compound. Unfortunatly many waste pipes in old sinks are made of lead...
Fortunatly I think you probably need to shove a lighted match at the compound to cause the explosion, but it is still a risk!
-Rosie-
QUOTE (Rosie @ Jan 9 2006, 12:24 PM)
Apparently (according to our tecnition) if it contacts lead then Sodium Azide forms an explosive compound. Unfortunatly many waste pipes in old sinks are made of lead...
Fortunatly I think you probably need to shove a lighted match at the compound to cause the explosion, but it is still a risk!
Fortunatly I think you probably need to shove a lighted match at the compound to cause the explosion, but it is still a risk!
Actually Lead (II) azide is very explosive. A fall of 15cm or a static electrical discharge will cause it to detonate. So a hammer tap would do the job. Lead azide is used in detonator caps. Copper (II) azide is even reactive. Getting these compounds wet, has little effect on their explosiveness.
However I believe the toxicity of sodium azide is a larger risk that its potential at reacting with lead piping.
(which naturally becomes internally coated with insoluble lead carbonate.)
-perneseblue-
Even terrorists know about the toxic effects which are very similar to cyanides. azide should be kept in locked shelves under strict supervision.
-Microbiologist-
QUOTE (praveenkumar thummala @ Dec 29 2005, 10:16 AM)
i want to know abt each and every chemical function in antibody purification .can anyony send abt this information please.
1.binding buffer(glycine,nacl&sodium azide)ph-8.6to9
2.elution bufferb1(citric acid(trisodium salt&anhydrous)&sodium azide)ph 5.5
3.elution buffer b2(glycine&sodium azide)ph-2.5-3
1.binding buffer(glycine,nacl&sodium azide)ph-8.6to9
2.elution bufferb1(citric acid(trisodium salt&anhydrous)&sodium azide)ph 5.5
3.elution buffer b2(glycine&sodium azide)ph-2.5-3
follow also an older discussion on thid forum:
http://www.protocol-online.org/forums/inde...804&hl=nan3
-The Bearer-
QUOTE (Microbiologist @ Oct 8 2006, 03:04 AM)
Even terrorists know about the toxic effects which are very similar to cyanides. azide should be kept in locked shelves under strict supervision.
Our azide is never locked in a storage cabinet...anyone can have acess to it.
-Minnie Mouse-