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CDNA Related Discussions
  1. Which DNA is good for PCR for amplify a certain sequence? cDNA or genomic DNA ? - (reply: 2)
  2. 1st strand buffer for cDNA synthesis - (reply: 1)
  3. How should products of first-strand cDNA synthesis look like on a gel? - (reply: 4)
  4. random primed cDNA: is there a lenght limit? - (reply: 3)
  5. cDNA dilution for efficency calculation - (reply: 1)
  6. RNA, cDNA, PCR - RT Inconsistency - High Quality RNA, Working RT, Varying PCR Results (reply: 2)
  7. cDNA primer help - (reply: 1)
  8. cDNA and primer Design Help - (reply: 6)
  9. synthesized cDNA - (reply: 3)
  10. How much oligo dT cDNA to use in PCR - (reply: 3)
  11. cDNA synthesis - (reply: 3)
  12. Determing the size of a cDNA library - (reply: 2)
  13. superscript indirect cDNA labeling system - superscriptIII, cDNA purification, microarray, free amines (reply: 3)
  14. cDNA library from invitrogen - (reply: 1)
  15. cDNA and additional bands when run on gel - I get a band o fthe same Mw regardless of the primers I use (reply: 2)
  16. low cDNA yield from bacterial total RNA. HELP! - (reply: 1)
  17. how much cDNA do you use for RT-PCR? - (reply: 2)
  18. Problems with second use of diluted cDNA in qPCR - (reply: 4)
  19. First strand cDNA synthesis for qRT-PCR - (reply: 1)
  20. cDNA storage - (reply: 3)
  21. How to design PCR primer to amplify a very long sequence(about 9.7kb) - I want to amplify cDNA genome of HCV. It's about 9.7kb long (reply: 2)
  22. genomic DNA PCR - is it the same as a cDNA PCR (reply: 9)
  23. spiking cDNA or PCR product for standanrd curve dilutions - (reply: 1)
  24. No cDNA from RNA sample - (reply: 4)
  25. Possible to sequence directly from cDNA? - (reply: 1)
  26. lentiviral systems for overexpression: clone in full cDNA or ORF? - (reply: 3)
  27. Standard curve from plasmid - for cDNA quantification (reply: 6)
  28. cDNA amplification- difficult! - (reply: 2)
  29. Purified cDNA Clonning - (reply: 3)
  30. is there simple method to know success first strand cDNA synthesis? - (reply: 1)
  31. Dilute your cDNA ? - for real time PCR (reply: 2)
  32. quantifying cDNA by spectrophotometry - (reply: 1)
  33. Comparative of Genomic and cDNA sequences - Intron sequences!!! (reply: 3)
  34. Questions about PCR amplification using cDNA (RT from total RNA). - (reply: 6)
  35. is cDNA sequencing tricky? - (reply: 1)
  36. RT-PCR from VERY limited quantity of tissue sample - Need suggestion on RNA isolation and RT kits to maximize cDNA yield (reply: 1)
  37. how to optimize primer and cDNA concentration for real-time - (reply: 2)
  38. cDNA quantification by non-PCR method - measuring RT efficiency (reply: 1)
  39. Double Stranded cDNA Low Yields - DS cDNA RT Yield Troubleshooting (reply: 2)
  40. getting long pcr from cDNA - (reply: 4)
  41. PCR from cDNA library - (reply: 1)
  42. Measuring cDNA concentration - (reply: 2)
  43. real time-pcr of cDNA samples - are the results reliable? (reply: 1)
  44. Inefficient RT-PCR, ¿bad cDNA? - don't know why (reply: 1)
  45. New to RNA... Does this cDNA gel look OK? - (reply: 2)
  46. purification of single stranded cDNA? - (reply: 3)
  47. cDNA library and molecular biology in general- am I in too deep - (reply: 3)
  48. better primer for cDNA synthesis in real time pcr - (reply: 1)
  49. conc of cDNA to be used - PCR to test primers (reply: 5)
  50. control for cDNA synthesis - (reply: 2)
  51. cDNA dilution - to dilute or not? (reply: 5)
  52. how to dilute cDNA from mRNA concentration? - (reply: 4)
  53. cDNA - (reply: 6)
  54. How to test if a cDNA clone from a plasmid is infectious? - (reply: 4)
  55. difficulty synthesising cDNA from total RNA - (reply: 26)
  56. generating a cDNA library - (reply: 1)
  57. Problems with cDNA - How long can I keep cDNA on 37°C? (reply: 3)
  58. cDNA library question - (reply: 1)
  59. How much cDNA from RNA toata?! - (reply: 2)
  60. cDNA stability after miRNA stem-loop RT - (reply: 2)
  61. cDNA quantification for PCR - (reply: 5)
  62. mRNA vs ds cDNA - Size differenses between mRNA and ds cDNA (reply: 3)
  63. Storage: cDNA or mRNA - (reply: 3)
  64. second strand cDNA synth without primer? - (reply: 2)
  65. RT-PCR cloning: oligo dT-primed cDNA synthesis or anchored? - (reply: 1)
  66. mrna size 2to construct cDNA libary - what is the size of mRNA Needed 2 work cDNA libary (reply: 1)
  67. cDNA - (reply: 4)
  68. Inverse PCR - Can I use cDNA to do ipcr? (reply: 4)
  69. construction of cDNA library - (reply: 2)
  70. Can I run q RT real-time PCR based on cDNA amount? - (reply: 1)
  71. primer design for unknown cDNA sequence - (reply: 1)
  72. EST vs. FL cDNA? - (reply: 6)
  73. cDNA library - Looking for the best company to order from (reply: 2)
  74. cDNA cloning using Taq/pfu polymerase - (reply: 2)
  75. quality of cDNA - (reply: 1)
  76. Cox-2 primer for PCR - method how to search good cDNA in Pubmed (reply: 1)
  77. Reverse Transcription, Heated Lid yes or no? - Is it necessary to use heated lid during cDNA synthesis incubations? (reply: 6)
  78. cDNA synthesis using random hexamer vs. oligo dt primers - (reply: 2)
  79. Reverse transcription validation and cDNA quantitation - (reply: 1)
  80. cDNA quantification using Nanodrop - (reply: 5)
  81. cDNA synthesis and statistical variation - (reply: 1)
  82. Can I use different amount of cDNA template to run real time PCR? - when the amount of templates are different,Can I use reference gene to (reply: 2)
  83. How to buy a cDNA clone? - (reply: 2)
  84. Checking cDNA concentration - Is it possible to check? (reply: 3)
  85. Case of the disappearing cDNA - (reply: 2)
  86. phage cDNA library screening with PCR - (reply: 3)
  87. Subtractive cDNA library - (reply: 4)
  88. pfu ultra and single stranded cDNA - (reply: 5)
  89. cDNA library insert-vector ratio - (reply: 1)
  90. Microarray with genomic DNA and cDNA at the same time - (reply: 1)
  91. How do I know if my cDNA are of good quality? - (reply: 7)
  92. cDNA cloning - (reply: 2)
  93. How big a problem is cDNA sticking to the plastic? - (reply: 3)
  94. Different sequencing result of PCR products from the same cDNA - (reply: 2)
  95. Degenerated qPCR data after keeping the cDNA in the fridge. - (reply: 2)
  96. cloning cDNA libraries - (reply: 4)
  97. Non repeatability of RT-PCR with same cDNA - (reply: 3)
  98. initial amount of cDNA - (reply: 2)
  99. How to know there is cDNA? - (reply: 5)
  100. cdna - (reply: 1)
  101. Reverse-transcription - how much RNA will be converted into cDNA (reply: 4)
  102. cDNA and PCR - enzimes (reply: 1)
  103. How to get the double strand cDNA from bacterial - (reply: 1)
  104. Custom made cDNA library? - Please name the companies who made the cDNA library. (reply: 4)
  105. Cloning a cDNA sequence - (reply: 4)
  106. contamination in cDNA - (reply: 4)
  107. respository for brain mRNA or cDNA - (reply: 4)
  108. about cDNA - (reply: 1)
  109. mistake in cDNA labelling - (reply: 1)
  110. Does non-digested RNA in cDNA sample have an effect on RT-PCR quantification? - (reply: 2)
  111. cDNA for real-time PCR - (reply: 3)
  112. cDNA for real time PCR - for gene expression study (reply: 1)
  113. what can i do with this pcr? - amplification of cDNA , with primers tm 50 and 67 (reply: 7)
  114. long size cDNA cloning - (reply: 1)
  115. gDNA contamination in cDNA library with cDNA creator smart kit - (reply: 1)
  116. optimization of cDNA template for real time PCR array - (reply: 2)
  117. Is multiple freezing-thawing harmful to cDNA? - (reply: 3)
  118. Problem on PCR from cDNA - (reply: 3)
  119. cDNA libraries - (reply: 2)
  120. different primer efficiency using cDNA and plasmid/PCR product - (reply: 4)
  121. RNA in cDNA - Can I use the same PCR primers for the production of cDNA? (reply: 3)
  122. use genomic DNA or cDNA? - (reply: 1)
  123. cDNA problem - (reply: 5)
  124. cDNA storage: Can we store cDNA for future use? - (reply: 3)
  125. RNA contamination in cDNA for microarray experiments - RNA contamination in cDNA for microarray experiments (reply: 3)
  126. difference between RNA & cDNA gel image - (reply: 1)
  127. Diluting your cDNA for qRT-PCR? - (reply: 5)
  128. Reamplification of cDNA - (reply: 6)
  129. synthesis of long cDNA - (reply: 2)
  130. Amplifying a 6.5 kb cDNA - (reply: 3)
  131. How much RNA is needed for labelling and how much labelled cDNA is needed for hy - (reply: 4)
  132. genomic library and cDNA - (reply: 2)
  133. cDNA sequences too similar to be real? - (reply: 2)
  134. does the quality od cDNA interfer in qRTPCR - (reply: 2)
  135. probelm with Full-length cDNA library construction - (reply: 5)
  136. cDNA synthesis for cloning - (reply: 3)
  137. elute cDNA on filter paper - (reply: 3)
  138. Concentration of cDNA after reverse transcription--no spec? - (reply: 2)
  139. cDNA -mRNA hybrid - (reply: 4)
  140. Amplification of gene (CHH,MIH,GIH) from prawns. - Amplification of gene using primers designed on the basis of cDNA sequences (reply: 1)
  141. cDNA tailing without terminal transferase - (reply: 1)
  142. cDNA synthesis primer - (reply: 3)
  143. cDNA synthesis ASAP - gene expression (reply: 3)
  144. How to check if reverse transcription went OK and there is cDNA - (reply: 5)
  145. Problems (unspecific products) with PCR to determine the size of cDNA inserts - (reply: 7)
  146. cDNA tailind - (reply: 3)
  147. qPCR, no RT controls always positive (as cDNA samples) - (reply: 4)
  148. insering cDNA before the tag.... - what problems should I consider? (reply: 17)
  149. affymetrix vs cDNA microarray conflicting results help - (reply: 1)
  150. Series dilution of RNA or cDNA for qPCR standard curve? - (reply: 1)
  151. pulling cDNA - a big error? - (reply: 6)
  152. need cDNA synthesis protocol - urgent (reply: 3)
  153. PCR my cDNA & got nothing - (reply: 2)
  154. cDNA library screening with 5' specific primer - (reply: 1)
  155. cDNA precipitate & DNA ladders - (reply: 4)
  156. HELP! cloning cDNA from double-stranded cDNA synthesis - (reply: 2)
  157. is cDNA concentration 200 ng/ul too high for qRT-PCR - (reply: 17)
  158. PCR problem: long product (4 kb) from a cDNA library - (reply: 2)
  159. quantifying cDNA for Real Time PCR - (reply: 3)
  160. Long RT for 4.5 kb cDNA - (reply: 5)
  161. Not enough cDNA for qRT-PCR - how to increase cDNA yield or save cDNA? - (reply: 12)
  162. RT issues and efficiency of cDNA synthesis - qRT-PCR (reply: 1)
  163. Is cDNA a single or double stranded ? - (reply: 7)
  164. Should I dilute cDNA for real-time PCR? - (reply: 3)
  165. Amplification of specific genes from cDNA - (reply: 3)
  166. Forgot cDNA at Room Temperature ?! - is it still usable ? (reply: 3)
  167. How to quantitate, to dose first strand cDNA ? - (reply: 20)
  168. cDNA library in pACT-vector - (reply: 2)
  169. How to remove genomic DNA contamination from a cDNA library-HELP! - (reply: 1)
  170. PCR on PCR of cDNA smear - (reply: 1)
  171. the joys of microarrays - how much cDNA do you expect to synthsise? (reply: 1)
  172. ordering a cDNA clone - found on the NCBI database (reply: 3)
  173. How can I get the 4.5kb Human typeI procollagen alpha1 chain cDNA Via RT-PCR fr - Seeking Help (reply: 14)
  174. RNA, cDNA, and DNA - RNA, cDNA, and DNA (reply: 3)
  175. Total cDNA PCR - (reply: 3)
  176. techniques to obtain full-length cDNA - (reply: 2)
  177. SuperArray: cDNA versus oligo - (reply: 1)
  178. Obtaining full length cDNA - Obtaining full length cDNA using Xenopus (reply: 4)
  179. cDNA cloning question - (reply: 7)
  180. cDNA library kit - library construction (reply: 3)
  181. Impure RNA - problem with cDNA? (reply: 3)
  182. cDNA synthesis from mRNA - (reply: 2)
  183. question about PCR clone a cDNA - (reply: 9)
  184. cDNA cloning problem - No product in PCR (reply: 5)
  185. how to select plant material to construct cDNA library? - (reply: 1)
  186. cDNA storage stability issues - cDNA storage (reply: 2)
  187. cDNA synthesis - Second Strand cDNA synthesis (reply: 1)
  188. cDNA size VS protein Size - MW weight confusion (reply: 7)
  189. Diluting of first strand cDNA sample - (reply: 2)
  190. Software for transformating RNA into cDNA - (reply: 1)
  191. cDNA is longer than mRNA? - ensemb's result (reply: 2)
  192. first strand or double strand cDNA before PCR: RT-qPCR - (reply: 3)
  193. T/A cloning of cDNA? - PGEM-T vs pCR 2.1 T/A TOPO? (reply: 3)
  194. "cDNA must accurately represent RNA input amounts" - (reply: 1)
  195. cDNA amount - (reply: 1)
  196. cDNA before qPCR-purifying or not?! - (reply: 2)
  197. cDNA - (reply: 4)
  198. concentration of cDNA after reverse transcription? - (reply: 5)
  199. transport RNA and cDNA - (reply: 2)
  200. gDNA, mRNA and cDNA sequences of a gene? - (reply: 5)
  201. Problems cloning cDNA using Pfx - Problem (reply: 2)
  202. Genomic DNA versus cDNA sequences - (reply: 1)
  203. How long does cDNA last for? - What's the longest time of storage of cDNA (reply: 2)
  204. Tissue and cDNA microarray? - (reply: 1)
  205. double sized PCR product from cloned cDNA - (reply: 6)
  206. CDNA clean up protocol - (reply: 2)
  207. Good links for cDNA microarray and RT-qPCR - (reply: 1)
  208. any cDNA websites? - (reply: 1)
  209. cDNA synthesis - which transcriptase is better? - (reply: 2)
  210. cDNA cloning by PCR - cloning (reply: 3)
  211. designing gene-specific primer for cDNA synthesis - (reply: 5)
  212. how to produce a high-quality cDNA template for RT-PCR - produce a high-quality cDNA template (reply: 4)
  213. cDNA quantification - (reply: 1)
  214. second strand cDNA - (reply: 4)
  215. RT product check - Can I qualify my cDNA? (reply: 2)
  216. cDNA from dsRNA - (reply: 1)
  217. Help! Problem on cDNA library construction - (reply: 2)
  218. any experience in cloning a 3.5kb full length cDNA - (reply: 4)
  219. Double strand cDNA sysnthesis - Using random primers (reply: 1)
  220. pcr select cdna subtraction - kit from clontech (reply: 1)
  221. degraded cDNA from immunohistochemically stained tissues - degraded cDNA from immunohistochemically stained tissues (reply: 1)
  222. cDNA PCR - Amplifying region using PCR from cDNA library (reply: 1)
  223. Degraded cDNA after freezing - (reply: 2)
  224. plant cDNA - (reply: 3)
  225. first strand cDNA probe - (reply: 1)
  226. PCR on cDNA libraries - (reply: 2)
  227. HELP!!! Having trouble with cDNAsynthesis! - (reply: 1)
  228. cDNA library (in Lambda  ZAP vector) screening - picking up plaques on nitro cellulose  membrane as replica (reply: 2)
  229. Too many positive signals fromimmunological screening of a cDNA librar - (reply: 1)
  230. first strand cDNA sythesis with high GC contents - (reply: 4)
  231. checking my cDNA - (reply: 1)
  232. How to prepare cDNA library fromsingle cells - (reply: 1)
  233. cDNA library construction - (reply: 1)
  234. cDNA library screening - (reply: 1)
  235. cDNA synthesis - cDNA synthesis (reply: 1)