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GFP Related Discussions
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EGFP cloning and membrane protein - (reply: 2)
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egfp in ecoli (pet60) - (reply: 1)
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Does FRET by BFP/GFP work? - (reply: 2)
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GFP can not be observed! Help. - (reply: 6)
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How to fix GFP expressing cell to preserve GFP signal in immunofluorescent stain - (reply: 8)
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High GFP background on FACS - (reply: 3)
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SOS help for GFP leakage during FACS - (reply: 2)
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Need help with transfection! - GFP + HSP (reply: 3)
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Question in EGFP saw fluorescence but read zero in fluoremeter - (reply: 1)
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Know good vector backbone for PT67? - Viral transduction of EGFP to epitherial cell line (reply: 1)
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What is the most sensitive assay for promoter strength? - dual luciferase assay? GFP? SEAP assay? Miller or something else? (reply: 3)
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GFP intercellular Localisation ; Helpneeded - (reply: 2)
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Cutting out EGFP gene from pEGFP-N1 - w/o using XbaI (reply: 2)
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cutting out EGFP gene from pEGFP-N1 vector - what does dam- mean? (reply: 1)
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Expression of GFP and DsRed tagged proteins - co-localization needed for FRET (reply: 9)
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half life time of GFP? - (reply: 1)
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help~ GFP fluorescence - (reply: 1)
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GFP tagged bacterial Cell preparation for FACS analysis - (reply: 2)
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GFP Tobacco BY-2 cell sorting - (reply: 1)
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Detection of GFP expressing cells - fix or not? - (reply: 3)
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observation of GFP fusion by LSCM in root tips - observation of GFP fusion by LSCM in root tips (reply: 4)
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N terminal GFP fusions - is it as appropriate as C terminus? (reply: 3)
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lentivirus transduction - no GFP after trasduction (reply: 5)
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quenching of GFP after selection of stable cells - (reply: 1)
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stable cell line, but quenched GFP? - (reply: 5)
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is my GFP quenched? - (reply: 2)
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design primers with custom end - To clone my gene into the MCS as fusions to the GFP tag (reply: 5)
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Will GFP interferes with luciferase assay result? - (reply: 3)
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HEK cell, EGFP and PI staning - (reply: 7)
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GFP western - (reply: 3)
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Can I use DAPI and GFP at the same time? - Need advice on fluorochromes for confocal microscopy (reply: 8)
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Imaging GFP tagged proteins, background signal due to contamination? - Imaging, background spots/speckles, contamination (reply: 6)
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EGFP stable cell line proof - (reply: 3)
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Cell Storage Conditions for Cell Cycle Analysis - PI staining with GFP containing construct (reply: 2)
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Living cell GFP imaging - (reply: 1)
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Living cell GFP imaging - (reply: 6)
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Replacing EGFP with RFP..but no expression! - (reply: 10)
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EGFP constructed in T-vector can express green fluorescence? - SOS (reply: 6)
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question on colocalization of mitochondrion and a GFP fusion - (reply: 2)
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colocalization of mitochondrion and GFP fusion - (reply: 2)
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after PFA fixation, EGFP intensity fated away over time - Anyone noticed this? (reply: 9)
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GFP, both in the nucleus and in the cytoplasm - transfection (reply: 8)
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no GFP in cells - (reply: 7)
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GFP imaging - (reply: 2)
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How can I transform GFP into recA -/- E. coli? - (reply: 1)
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brdu staining on gfp+ve cells - (reply: 1)
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BrDU staining on GFP positive cells - (reply: 1)
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No expression from injected GFP&RFP mRNA - (reply: 16)
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Stable transfection with or without EGFP - (reply: 3)
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How to get GFP producing bacteria? - help? (reply: 5)
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How to quantify GFP fluorescence from a bacteria? - (reply: 3)
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GFP/RFP/YFP G418 resistant plasmids - Help please (reply: 1)
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Destroying GFP fluorescence - (reply: 5)
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Reduction in EGFP after sorting - (reply: 4)
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Has anybody added artificial introns into GFP coding sequence. - (reply: 1)
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constrcut a GFP plasmid that work in Drosophila s2 cells - (reply: 1)
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Good GFP antibody from SANTA CRUZ for Western Blotting? - (reply: 7)
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Problem with gfp express in S2 - (reply: 7)
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Detection of GFP in supernatant - (reply: 2)
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Sindbis virus expression - cannot detect EGFP signal in hippocampal neurons (reply: 1)
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gus or gfp - gus or gfp (reply: 2)
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FACS GFP leakage - (reply: 4)
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How get GFP signal without mRNA target from pIRES-EGFP - positive by FACS but no DNA amplifation from RT-PCR (reply: 8)
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EGFP fusion protein expression - Expression of a GFP fusion protein in E.coli (reply: 4)
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Loss of GFP - (reply: 2)
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using EGFP fluorescence as measure of transfection efficiency - (reply: 3)
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HELP! GFP plasmids - GFP plasmids (reply: 4)
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half life of EGFP protein - (reply: 3)
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GFP transfection - basic protocol needed (reply: 1)
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loss of GFP upon selection - (reply: 4)
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a question about screening transfected cells by GFP protein - (reply: 5)
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GFP GOLGI - Expresion of pAcGFP Golgi. (reply: 3)
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gfp fusion proteins - is liker needed if so how many amino acids usual choice? (reply: 2)
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Help! GFP transfection - (reply: 12)
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GFP siRNA transfection - readout - (reply: 5)
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GFP Fusion protein - (reply: 6)
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GFP to expression - (reply: 3)
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why does empty EGFP vector has no fluorescence? :/ - (reply: 5)
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why EGFP become weak in C2C12? - (reply: 3)
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Expression of GFP fusion protein - Improving fluorescence visualization (reply: 2)
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"Phantom" GFP bands - (reply: 2)
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Help! GFP is bleached after immunostaining. - (reply: 2)
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immunostaining but GFP is bleached - (reply: 1)
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Problem with EGFP in translation study - (reply: 12)
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GFP labelled protein purification - (reply: 1)
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GFP visualisation in GloFish - (reply: 1)
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Detection on plate for a bacteria who express GFP? - (reply: 7)
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gfp digested with Ecor1 + Hind III, and Bgl II - (reply: 4)
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GFP and DsRed dual lenti construct - (reply: 4)
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BrdU incorporation and GFP staining - (reply: 3)
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EGFP and GFP - how different are they? (reply: 8)
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Fixation for eGFP imaging? - what fixation should I use? (reply: 3)
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GFP expression - microscopy, spectrofluorimetry (reply: 3)
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anti GFP - IP - (reply: 3)
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GFP expressing cells - masking of GFP expressing cells!!! (reply: 6)
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can i still see the GFP reaction after the distruction of cell. - (reply: 1)
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Protocol:GFP reporter assay - (reply: 4)
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Stop codon before GFP - Stop codon before GFP (reply: 7)
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SiRNA and GFP transfection MCF7 cells - (reply: 4)
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Replacing of GFP cloned inside a gene with RFP - (reply: 2)
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picked GFP transfected colonies do not retain flouresence after being picked - (reply: 3)
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dsRNA of EGFP as control? - (reply: 4)
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transfection gfp visualization - (reply: 14)
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Expression of GFP fusion: splicing out of tag ? - (reply: 1)
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GFP as a reporter gene? - (reply: 5)
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Transfection efficiency by GFP - (reply: 4)
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Cell cycle analysis (FACS) analysis and GFP detection - (reply: 10)
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Join GFP to my insert without mutagenesis - (reply: 8)
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GFP or LacZ for quantification of promoter activity? - (reply: 4)
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detection of GFP fluorescence in whole cell bacteria - (reply: 1)
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Expression of N-terminal GFP fusionproteins - Proteins with N-terminal signalpeptide (reply: 3)
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Localization of GFP - (reply: 2)
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Half life of EGFP - (reply: 1)
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Site directed mutagenesis GFP to RFP - (reply: 4)
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destabilized EGFP Vector urgently needed - (reply: 4)
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siRNA /shRNA agains EGFP - (reply: 4)
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Transfection, GFP, FACS and UV Microscope - great inconsistency b/t FACS and UV microscope (reply: 3)
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EGFP detection - (reply: 4)
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GFP protein not soluble? - (reply: 1)
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pGen.IRES.GFP.Neo - (reply: 3)
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FACS: DNA content and GFP in one go!!! - PI masks GFP??? (reply: 4)
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Problems with co-localization (GFP+lysotracker red) - (reply: 2)
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How to sort real GFP positive cells? - autofluorescence VS GFP (reply: 2)
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Cloning into GFP vector - (reply: 2)
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invisible GFP - (reply: 2)
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cloning problem - cloning problem in GFP vector (reply: 4)
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western blotting, (GFP is there - But where's the protein I want?) (reply: 3)
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Ligation into EGFP problems - Ligation into EGFP problems (reply: 5)
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GFP - (reply: 1)
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DNase,BrdU and GFP - (reply: 1)
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GFP transfection using lipofectin - (reply: 1)
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GFP ELISA essay - (reply: 1)
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Green Fluroscent Protein - Identification and Characterization of Promoter usin GFP (reply: 1)