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Transfection Related Discussions
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Gene promoter - Luciferase reporter assay help - (reply: 3)
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Plasmid distributors for Luciferase Assay? - (reply: 2)
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Transfection Vs Transduction - (reply: 1)
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Neuron transfection - (reply: 1)
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Viscous plasmids and transfection issues - (reply: 2)
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Transfection - (reply: 2)
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Transfection help - (reply: 1)
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Purification of linearized AT-rich DNA for transfection - (reply: 4)
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transfection - (reply: 2)
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Lipofectamine 3000 Protocol Written Out With Timing Specifics Regarding the Tran - (reply: 1)
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Luciferase assay - experimental design - (reply: 1)
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Transfection with Lipofectamine and PEI, differences - (reply: 2)
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Luciferase assay cloning - (reply: 2)
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What make a plasmid candidate for stable transfection - (reply: 1)
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Transfection with lipofectamine - (reply: 1)
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Why are my Huh 7s dying after transfections? - (reply: 1)
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Storing Ligated/Transformation Material before Transfection - (reply: 1)
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transfection of suspended CHO cells - (reply: 2)
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Hek293 transfection help!!!! - (reply: 1)
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why is my cells dying in the centre of the wells after transfections - (reply: 1)
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BJAB cells: does anyone have a specific protocol for transfection? - (reply: 1)
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Help with HUVEC cell transfection - (reply: 3)
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MDA-kb2 cells - problem with luciferase expression - (reply: 6)
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GFP interference in dual luciferase assay? - (reply: 2)
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preparing a vector for transfection - (reply: 2)
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Easy to transfect primary cell line? - (reply: 1)
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How long after transfection or dox induction of stable cell line before performi - (reply: 1)
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transfection of cells with two fluorescent reporters - (reply: 1)
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Stably transfected cells start dying - (reply: 6)
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not successful with transfection - (reply: 5)
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Transient transfection in huvec - (reply: 1)
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Blasticidin dose for Transfected - (reply: 1)
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Transfection problem - (reply: 6)
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Transfection media change - (reply: 1)
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Transfection of SH-SY5Y cells - (reply: 4)
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help for chip assay and luciferase assy - (reply: 1)
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Transfection help - (reply: 4)
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Viral RNA in total RNA eluate for transfection - (reply: 1)
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Stability of transient shRNA transfection - (reply: 1)
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Has anyone experience in isolating single-cell clones of CHO transfected cell li - (reply: 4)
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Relationship between plasmid size and amount of lipofectamine needed? - (reply: 1)
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Lentivirus transfection using RAW 264.7 cells - (reply: 2)
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DAPI stainged dots only in cells transfected with Fugene6 - (reply: 5)
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293T cell transfection troubles - Lipofecamine 2000 - (reply: 3)
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Transfection Enhancer Efficiency - (reply: 1)
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Transient transfection using linear 25 kDa PEI transfection reagent in CHO k1 - (reply: 4)
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CHO-K1 transfection problem - (reply: 2)
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Using pBABE plasmids for regular transfection - (reply: 1)
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Subsequent DNA and siRNA transfection - (reply: 9)
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need help for transfection to synchronized cells - (reply: 4)
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293t transfected with prtTA inducible vex - (reply: 5)
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No expression in stable transfected cell line - (reply: 2)
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Dead cells after transfection - (reply: 9)
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preparation of cells for FACS analysis after transfection - (reply: 4)
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Protein fragment is alwasy degraded post transfection 48 hrs - (reply: 2)
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Transient transfection with large plasmid - (reply: 3)
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transfection using lipofectamine 2000 - (reply: 3)
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Determining phosphorylation status of a protein by transfection - (reply: 5)
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SH-SY5Y stable cell transfection and RA differentiation - (reply: 1)
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PGL-3 Luciferase Inactivity - (reply: 2)
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What is the optimal time for transfection efficiency measuring? - (reply: 1)
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Rate of protein production with pcDNA 3.3 transfected into HEK293 - (reply: 3)
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question about sequential transfection several plasmids in 293T and COS7 cells - (reply: 1)
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Verified Plasmids(via digestion) > Transfection and Visible GFP > GFP de - (reply: 2)
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Transfection but no expression - (reply: 4)
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Cells Detach After Transfection - (reply: 1)
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No reduction in protein expr after 2nd passage of transfected cells - (reply: 3)
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Clone selection for scrambled shRNA transfected cells ? - (reply: 2)
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Transfection methods for human ESCS of iPSCs - (reply: 1)
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Simultaneous RNA and DNA transfection? - (reply: 2)
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death of transfected cells after first passage in G418 medium - (reply: 1)
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transfection with lipofectamine 2000 advice - (reply: 4)
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clonogenic assay for shRNA transfected cells - (reply: 3)
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Luciferase fusion protein? - (reply: 1)
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How did they do this? Microinjection? Transfection? - (reply: 2)
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Luciferase assay problem - (reply: 2)
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Transient transfection of linearized plasmids - (reply: 3)
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HUVEC plasmid transfection challenges - (reply: 2)
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Magnet assisted transfection - (reply: 1)
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Trouble with shRNA transfection - (reply: 2)
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Transient Transfection Controls (Gateway Technology) - (reply: 4)
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Geneticin Resistant Cell Lines and Stable Transfection - (reply: 1)
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To small amount of cells used for shRNA transfection - (reply: 1)
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Dual Luciferase Assay - (reply: 1)
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Determining the amount of plasmid transfected - (reply: 1)
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Can I scale up at transfection, after determining the drug concentration via kil - (reply: 2)
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Luciferase assay - extreme variation in RLU - (reply: 1)
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qPCR from HEK cells transfected with Plasmid - (reply: 4)
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Insect cells transfection reagent - (reply: 1)
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primary valve interstitial cells transfection - (reply: 1)
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Problem in transfection for producing lentivirus - (reply: 4)
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Transfection of a non human gene into human cell line - (reply: 1)
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Transcription factors involved in mouse lung transfection - EMSA, pull-down- how - (reply: 3)
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Lipofectamin 2000 and Large Plasmid Transfection Optimization - (reply: 3)
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Recommendation on siRNA transfection reagents - (reply: 2)
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lipofectamine 2000:DNA complex stability - (reply: 2)
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Transfection reagent stored at -20C - (reply: 2)
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DNA and siRNA transfection and cytotoxicity - (reply: 1)
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Accessing Transfection efficiency using fluorescence microscope (Alexa Fluor 555 - (reply: 1)
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transient transfection problem using ME180 cells - (reply: 1)
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Linearising plasmid before transfection? - (reply: 2)
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Transfection of pCI-neo plasmid into 3T3-L1 cells - (reply: 1)
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DMEM or EMEM Does it matter? (HeLa Cells & Transfection) - (reply: 3)
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Fugene transfections - (reply: 1)
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SF9 transfection with siRNA - what transfection reagent has worked for you? - (reply: 3)
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Protocol for HT29 siRNA transfection using Lipofectamine 2000? - (reply: 3)
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Cell confluency, transfection, protein expression for 293T cells - (reply: 2)
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Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
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3UTR luciferase assays - Genecopoeia or Swithcgeargenomics? - (reply: 1)
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Dual Luciferase assay data analysis issues - (reply: 4)
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HEK 293T Transfection problem - (reply: 4)
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gene overexpression in cells: virus transduction or transfection following selec - (reply: 4)
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Luciferase: too much pbs - (reply: 4)
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Transfection Rate - (reply: 1)
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Calcium Phosphate transfection - storage? - (reply: 1)
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Calcium chloride plasmid transfection - (reply: 1)
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virus transduction vs plasmid transfection - (reply: 4)
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Luciferase Assay Weird Result: TK-Renilla Changing More than SV40 Firefly - (reply: 1)
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transient transfection of retroviral vector without retroviral particle porudcti - (reply: 1)
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Transient Transfection: Adherent vs. Suspended/Floating Cells - (reply: 7)
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Stable transfection - (reply: 2)
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Luciferase assay-normalisation - (reply: 2)
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Seeding HeLa cells into 96 well plate for transfection - (reply: 2)
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Transfection with T-PRO reagent - Any tips? - (reply: 1)
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Cotransient Transfections DNA Requirements - (reply: 3)
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Help with Dual luciferase assay - (reply: 1)
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How to Dilute Cell Lysate for Luciferase Assay - (reply: 2)
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Luciferase assay - expected range of raw reading values - (reply: 1)
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Calculating siRNA Transfection Efficiency - (reply: 3)
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LENTIVIRUS transfection efficiency - (reply: 1)
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Transfection problem after transformation! - (reply: 5)
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Transfection - (reply: 1)
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Transfection of SH-sy5y with lipofectamine 2000 or electroporator - (reply: 1)
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Measure transfection effiicency - referee's question - (reply: 1)
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cells die after transfection - (reply: 1)
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What is cotransfection in molecular cell biology? - (reply: 1)
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stable transfection - (reply: 1)
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Chloroquine use in transfection!!! - (reply: 2)
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Problems with double transfections -cells die - (reply: 7)
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NIH 3T3 Stable Line Transfection - (reply: 1)
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GFP and luciferase measurements - (reply: 3)
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extract plasmids from transfected eukaryotic cells - (reply: 6)
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identification of the transfection efficiency - (reply: 4)
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stable transfection suspension cultures - (reply: 1)
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problem with transient transfection - (reply: 6)
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Problem in Stable transfection - (reply: 3)
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Transfection & Cilia help! - (reply: 3)
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Storage of transfected PC12 cells - (reply: 1)
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Transfection - Optimising Transfection Protocol (reply: 2)
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Help to construct luciferase DNA construct - construct luciferase DNA construct (reply: 2)
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problem with siRNA transfection of HEK cells - (reply: 5)
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Viral transfection to neurosphere in vitro - (reply: 1)
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What transcription factors are involved in transient transfection - (reply: 1)
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NMDA Transfection in 293 Cells - (reply: 1)
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Lipofectamine and transfection - (reply: 2)
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problems at normalizing luciferase assay data - (reply: 1)
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dual luciferase data analysis - (reply: 2)
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renilla values are going down with firefly values after miRNA transfection - (reply: 1)
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Dual luciferase assay - proper controls? - LUC below pGL3 despite protein activity (reply: 4)
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Big plasmids and Transfection - (reply: 3)
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CaMKII alpha promoter and cell tranfection - (reply: 1)
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How to explain this luciferase result?! - (reply: 2)
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Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
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MDA-MB-231 cell transfection - (reply: 5)
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siRNA transfection advise - Need some help on siRNA knockdowns (reply: 1)
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why these guys use T7 instead of CMV? - for transfection (reply: 2)
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Luciferase assay - (reply: 2)
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NIH3T3 Transfection - (reply: 1)
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Help! Transfection newbie with a dumb question - Trying to transfect adherent cells (reply: 1)
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siRNA transfection with lipofectamine 2000 - (reply: 1)
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Will transfected plasmids induce cytokine production? - (reply: 1)
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Help with HELA and HEK293 cell seeding for transfection - (reply: 4)
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Transfection in insect cell lines?? - (reply: 1)
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is there a method to set up stable transfectants without drug selection - no drug-resistant gene (reply: 8)
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ShRNA transfection - (reply: 1)
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Luciferase Assay Stim. Concentration? - (reply: 1)
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miRNA/siRNA transfection doubts - (reply: 2)
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Crosstalk between luciferase and GFP in Dual Luciferase Assay (Promega)? - (reply: 2)
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TE buffer in PEI transfection - (reply: 1)
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problems in lentiviral transduction - time to collect, ratio for transfection (reply: 1)
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Virus with serum - viral transfection (reply: 1)
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Medium used after transient transfection? - serum free medium or medium with serum (reply: 1)
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Neuron transfection method-need the best protocol - (reply: 4)
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3'UTR luciferase assay with 5 miRNA target sites - how to design 5 miRNA target sites in the 3'UTR (reply: 2)
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transfection or infection - difference? (reply: 1)
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DharmaFECT transfection reagent - (reply: 1)
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stabile transfection, all have high mRNA level, only ~ 50 % have protein - (reply: 1)
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luciferase GPCR assays - (reply: 1)
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Problems with transfection in human cells - (reply: 3)
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Preferences for MEF transfections - (reply: 1)
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CaPO4 transfection - (reply: 1)
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Transient transfection with viral vectors - Is it acceptable to do direct transfection with viral vectors? (reply: 1)
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microRNA knockdown its target by western blot but can't inhibit the 3'UT - want a reliable protocol to do the 3'UTR luciferase assay (reply: 3)
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293 Transfection Troubleshoot ! - (reply: 2)
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Luciferase and cell responsiveness - (reply: 3)
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Transfection of large plasmid - How to get a 10kb plasmid into HEK293 cells (reply: 3)
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Transfection Problem - (reply: 3)
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293T transfection - (reply: 1)
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lenti virus transfected cell dies... - (reply: 4)
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how to compensate the cell death during transfection by electroporation - (reply: 2)
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how to minimize the variation among the replicas - transcient transfection (reply: 4)
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Transfection - (reply: 1)
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normalization of dual luciferase assay - (reply: 4)
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dual luciferase reporter?? - (reply: 2)
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Most Effective Transfection Reagent for MDA MB 231 - (reply: 1)
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Homemade Renilla Luciferase Substrate? - How to make Renilla Luciferase Substrate (reply: 1)
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Methylation and luciferase reporter query - (reply: 4)
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why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
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tranfection of shRNA into mammalian cells is causing the cells to die - (reply: 4)
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transfection efficiency - i am looking for a cell line that its transfection efficiency is high (reply: 4)
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explain Transient transfection - (reply: 1)
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Help! low efficiency of transfection - (reply: 2)
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pre-miRNA (ambion) transfection troubleshooting - I am getting opposite results than expected (reply: 6)
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Stable transfection selection - (reply: 3)
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transfection is not working - (reply: 4)
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Transfection didnt work after repeating it. - (reply: 11)
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Retrovirus production - How to produce retrovirus with Transient transfection? (reply: 2)
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Changing the media after transfection of 293T cells - I did not change the media the day after transfecting 293T cells. (reply: 7)
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Non Mouse / Human Cell Line that is easily transfected - (reply: 2)
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suspension cell transient transfection - (reply: 10)
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After Transfection - (reply: 1)
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Will mycoplasma contamination lower transfection efficiency? - (reply: 10)
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Early transfection - (reply: 6)
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Plant cell transfection - (reply: 1)
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luciferase - (reply: 1)
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cloned plasmid expression less than original plasmid - after transfection (reply: 4)
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Transient transfection efficiency too good? - Luriferase reporter assay (reply: 2)
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Regarding methylation and luciferase assay - Regarding methylation and luciferase assay (reply: 4)
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Basic questions about Transfection - About Lipofectamine (reply: 3)
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GFP and Luciferase Cell Line - (reply: 2)
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Changing media after transfection - When??? - From Serum containing media to serum free media (reply: 2)
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Luciferase signal - (reply: 5)
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Transfection into eukaryotic cells - (reply: 1)
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Lentivirus Production: Problems / Transfection Method - (reply: 4)
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Normal 96-well compatible for luciferase assay? - (reply: 3)
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Transfection - (reply: 1)
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Double transfection of siRNA - (reply: 2)
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NIH3t3 transfection optimization - (reply: 1)
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Co-transfection of luciferase reporter plasmid and constitutively expressed beta - (reply: 1)
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problem in plasmid transfection - (reply: 2)
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Cotransfection of siRNA and plasmid - Cotransfection of siRNA and plasmid (reply: 1)
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Calcium Phosphate Transfection for CV1 CELLS - (reply: 2)
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How to normalize my samples before I transfect my cells - (reply: 6)
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transfection efficiency - (reply: 1)
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transfection time - (reply: 2)
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problem in transfection - (reply: 4)
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No difference between transfected and untransfected cells - Using eGFP siRNA on GFPHEK293 cells usign flurimeter (reply: 5)
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transient transfection - (reply: 1)
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stable transfection problem - (reply: 1)
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Luciferase transfection controls - (reply: 3)
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checking transient transfection efficiency - (reply: 3)
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Immunofluorescence with transfected cells - (reply: 3)
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GFP constructs in Luciferase assay - (reply: 4)
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Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
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suspension cell transient transfection - (reply: 2)
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cell cryopreservation after siRNA transfection - (reply: 1)
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Assessing Pre-miR transfection efficiency - Without a fluorescent microscope (reply: 2)
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lipofectamine and PEI transfection of hela cells - (reply: 2)
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Hoe much media needed for transfection complex? - transfection (reply: 9)
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Neon Transfection System - (reply: 2)
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Transfection before plating? - (reply: 2)
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Optimizing transfection of miRNA - Need help on transfection using lipofectamine 2000. (reply: 2)
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Half-life of Transfected pre-miRNA and miRNA inhibitors - (reply: 2)
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Calcium Phosphate transfection help - cells detaching after media change (reply: 1)
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Transfection and WB - Transfected cell line shows incorrect bands on WB (reply: 2)
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nonspecific band in transfected c-abl cells - nonspecific band in transfected c-abl cells (reply: 1)
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Dual Luciferase Assay - (reply: 3)
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Transfection - Plasmid Uptake Control - (reply: 2)
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Luciferase GL4 siRNA - (reply: 5)
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Two virus method for transfection - (reply: 1)
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luciferase assay - basic information on luciferase assay (reply: 3)
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plasmid purification kit for transfection - (reply: 2)
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best plasmid purification kit for transfection - (reply: 1)
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Difficulties with the transfection of cells CHO DG44 - (reply: 9)
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Transfection: How long until translation starts? - (reply: 2)
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Can Morpholino oligo be delivered in oocytes by lipofectin - (reply: 2)
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Please Help! Transfection: dropwise addition of lipid -dna complexes - (reply: 5)
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How to design the 3UTR seq for luciferease Assay - whole 3UTR of part of 3UTR for luciferase assay (reply: 1)
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Tranfection cell density and overgrowth - (reply: 3)
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Double transfection questions - (reply: 2)
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U2OS transient transfection - (reply: 6)
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Liver cancer cell transfection via lipofectamine - Liver cancer cell transfection method (reply: 3)
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How good are CHO cells - in general and for transfection (reply: 1)
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Transfection Efficacy - FAM-labelled NT or Lamin? (reply: 2)
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Promoter Cloning for Luciferase Assay - (reply: 3)
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siRNA and plasmid cotransfection - (reply: 1)
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Tranfection of HL 60 cells - (reply: 1)
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Co-transfection of two plasmids - Need the protocol for lipofectamine 2000 based transfection (reply: 5)
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Transfection of NIH/3T3 - (reply: 2)
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Transfection problems - (reply: 5)
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expression of SV40 in luciferase assay - (reply: 3)
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Minimum transfection incubation time - (reply: 2)
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Transfection - (reply: 7)
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about measuring luciferase activity in living cells - (reply: 9)
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MCF-7 cells dying after siRNA transfection + estrogen - (reply: 1)
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Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
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Is it possible to reuse electroporation cuvette to transfect siRNA - (reply: 4)
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Chemical transfection with Calcium Chloride - anyone used this method and made the 2X HBS? (reply: 6)
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Contamination in Transfection Agent - How do I decontaminate transfection agent? (reply: 7)
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transient transfection - (reply: 2)
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Transfection Agent - Lipofectamine 2000 VS Lipofectamine (reply: 1)
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HepG2 siRNA transfection - (reply: 1)
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Lipofectamine transfections once working now dying - Optimised transfection in HEKs starting to cause cell death (reply: 6)
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Problems with stable transfection - Cells transfection with alpha synuclein insert using electroporator (reply: 3)
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Max. length for 3'UTR in luciferase assay? - (reply: 1)
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Would transfected plasmids bind histones in HeLa cells? - h3k4 demethylase LSD1 (reply: 3)
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Transfection Issue - Are MCF-7 & MDA-MB-231 cells generally difficult to transfect? (reply: 2)
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Luciferase assay - (reply: 3)
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Transfection problem - milky media - (reply: 1)
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stable transfection problems - stable knockdown (reply: 3)
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Luciferase reporter induced by betagal vector?? - (reply: 1)
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HeLa tranfection- Dual luciferase Assay - (reply: 2)
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transfection in floating cells - (reply: 5)
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Transfection Expression Level Issues - (reply: 6)
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Is there optimal value for firefly and Renilla Luciferase transfection? - (reply: 1)
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complicated transfection requires professional help - (reply: 3)
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transient transfection efficiency - (reply: 2)
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Filtration DNA before transfection? - (reply: 4)
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293s, transfection and MTT assay - help! (reply: 3)
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Transfection efficiency vs. amount of plasmid DNA - (reply: 1)
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pIRESneo for mammalian cells transfection - (reply: 4)
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How to transfect two plasmids in a cell - (reply: 9)
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SKBR3 transfection - (reply: 1)
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lipofectamine - (reply: 3)
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fibroblasts transfection - (reply: 1)
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Firefly Luciferase Assay - variation in FL reading (reply: 2)
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cell line for luciferase assay - How to choose the best cell line? (reply: 2)
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transfection - (reply: 1)
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transfection - (reply: 1)
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Heterogenous expresion of stably transfected gene - (reply: 4)
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Retroviral vector with luciferase - (reply: 1)
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Transfection efficiency determination - (reply: 4)
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Transfection contamination? - (reply: 12)
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Luciferase assay for Wnt/b-catenin pathway - method misunderstanding (reply: 1)
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siRNA transfection of RS4-11 and MV-4-11 Cells - (reply: 1)
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stable transfectants - (reply: 6)
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Quick lipofectamine question - (reply: 7)
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Transfection AML-193 - (reply: 3)
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stable 3t3l1 transfection:serious help needed!!! - (reply: 10)
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Luciferase Assay Troubleshooting - Variable RLU from Renilla (reply: 1)
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Make construct so it is only thing that can PCR after transfection - (reply: 2)
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Gencarrier-1 vs. CaPO4 transfections - (reply: 4)
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Target gene regulation by transiently transfected PR - (reply: 4)
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Transfection of vectors - (reply: 8)
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Lipofection transfection of HEK293 cells unsuccessful - (reply: 10)
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Bovine cell line for transfection... - Transfection friendly cell line for localization studies... (reply: 1)
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Define Trasnsfection efficiency interms of siRNA experiment - Sirna uptake Vs transfection efficiency (reply: 12)
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SF9 cell transfection using lipofectamine?? - and is CMV active in this cells? (reply: 3)
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Hygromycin selection - Selection 72 hours after transfection (reply: 3)
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293Flp cell transfection - Passaging cells 4-6 hours after lipofectamine trasnsfection (reply: 3)
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stabel Transfection of Jurkat cells - G418 Selection (reply: 2)
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In vivo transfection of plasmid DNA, how? - intramuscular injection DNA (reply: 2)