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Staining Related Discussions
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Staining DNA and blotting - (reply: 1)
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Can't remove Ponceau staining! - (reply: 2)
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Gomori trichrome stain - (reply: 1)
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TRAP staining decalcified bone paraffin embedded, osteoclasts disappear - (reply: 2)
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Intestine tissue falling of slides during H and E staining - (reply: 1)
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How to prep mouse urine samples for SDS-PAGE and Coomassie staining? - (reply: 2)
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unusual FITC background staining - (reply: 2)
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lower part of the gel was not stained - (reply: 7)
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Can microglia stain positive for phagocytosed material? - (reply: 2)
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Cytotoxicity of hoechst staining in overnight experiment - (reply: 1)
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Gram staining issues - (reply: 4)
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Use of fluorescent stain on IEF gels - (reply: 1)
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Annexin PI staining - (reply: 1)
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immunofluorescence staining protocol question - (reply: 1)
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protein staining with EtBr!! - (reply: 4)
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Syber green gel staining after electrophoresis - (reply: 4)
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Trypan Blue Staining - (reply: 1)
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All bands, including ladder, appear as double bands in EtBr stained gel - (reply: 6)
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Why Glycomacropeptide (GMP) does not bind Coomassie Blue stain? - (reply: 2)
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Use of Gram Stain kit with Reagents differentation - (reply: 2)
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Troubleshooting SDS-PAGE: turquoise Area after Coomassie staining - (reply: 2)
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Staining SDS-PAGE gel with EtBr? - (reply: 6)
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polyA RNA staining for dot blot - (reply: 4)
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cell viability with PI staining - (reply: 2)
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silver stain destaining - (reply: 1)
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Spotted DAB staining! - (reply: 1)
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Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
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Quantuitative staining of RNA and DNA? - (reply: 4)
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Karyotyping, Giemsa Staining - (reply: 4)
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How to differentiate Enterobacteriace on Gram Stain? - (reply: 3)
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Is possible that unknown culture came out negative for all the three staining te - (reply: 2)
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why EEA1(early endosome antiboy) stained to nuclei? - (reply: 1)
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Quantification of the immunohistochemical staining slides - (reply: 4)
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FITC conjugated phalloidin staining of cells on microcarrier beads - (reply: 1)
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In simple staining, what is the charge of the dye? - (reply: 1)
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PI staining cell cycle analysis - (reply: 1)
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Can I use an EtBr stained PAGE gel for northern blot? - (reply: 2)
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2 Photos - 2 Questions: Gram Stain - (reply: 4)
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H&E Staining help! - (reply: 1)
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Immunofluorescence Staining Problem of Mitotic Cells - (reply: 2)
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Flow cytometry and immunofluorescent staining: help needed - (reply: 2)
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Low signal, weird background stains on blot - (reply: 5)
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Immunohistochemical estrogen/progesterone receptor staining for CELLS - (reply: 1)
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Live cell sort DNA stain permeabilization - (reply: 1)
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Gram Staining: What can one see? - (reply: 3)
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PE and FITC stain after fixing cells with 4% PFA - (reply: 3)
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Incomplete Blotting and staining of NC membrane - (reply: 7)
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Intracellular staining at 4C - HELP - (reply: 8)
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polyacrylamide gel with sybr green staining - (reply: 1)
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Intracellular staining of cytokines... crossreactivity? - (reply: 1)
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immunofluorescence - antibody choice for double staining - (reply: 2)
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Troubleshooting with silver staining - (reply: 3)
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unidentified objects in my cells, no staining.. what are they? - (reply: 2)
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Acid fast staining: need help - (reply: 5)
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DAPI staining in fixed Drosophila Hemocytes - (reply: 1)
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GFP staining with FACS - (reply: 1)
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co-IP stained with coomassie blue - (reply: 2)
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Losing cells during intracellular staining method! - (reply: 1)
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Smeared Bands after Ponceau Staining? - (reply: 3)
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coomassie blue stain - (reply: 1)
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How do you reduce crystal violet staining background on plates? - (reply: 1)
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Transwell migration assay (Boyden chambers) and DAPI staining - (reply: 1)
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PI cell cycle analysis and apoptosis - or would a different stain be better? - (reply: 2)
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Stain in electrophoresis is going from + to - - (reply: 3)
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Immunofluorescence: simultaneous polyclonal and monoclonal staining to same prot - (reply: 1)
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Looking for simple Papanicolaou (pap) staining protocol - (reply: 2)
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Weird small spheres stained by DAPI (not nuclei!) in tissue - (reply: 1)
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Stain nitrocellulose membrane with coomassie stain? - (reply: 3)
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Problem with Gamma H2AX foci staining - (reply: 5)
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Cell number issue - Nile Red staining - (reply: 3)
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Western blot and immunofluorescence staining - (reply: 9)
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Integrin B1 Staining in HT1080 cells - (reply: 1)
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Intracellular staining, isotype control not stable - (reply: 1)
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isoelctric gels hard to stain? - (reply: 2)
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Alizarin Red Staining on scaffolds... - (reply: 1)
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Mass-spec compatible silver stain - (reply: 2)
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viability/integrity stain of fungal cells - (reply: 5)
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NBT solution in X-Gal staining - (reply: 1)
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HELP-zebrafish antibody staining after day3 - (reply: 6)
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Please look at my gel picture, poor EthBr staining - (reply: 2)
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protein not stained by Coomassie Blue - (reply: 13)
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Flag Staining in U87 cells - (reply: 4)
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Cells detach during bodipy 493/503 staining - (reply: 1)
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Problem with sequential double IF staining with rabbit antibodies - (reply: 2)
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Distinguish murine and human cells by surface staining? - (reply: 1)
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Non-fluorescent live/dead stains for prokaryotes - (reply: 2)
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Live/Dead Staining of already Permeable Cells - (reply: 10)
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PVDF membrane silver staining protocol - (reply: 5)
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Alcian Blue and Silver Staining for CPS and LPS - (reply: 3)
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Coomassie stain forzen and thawed gel? - (reply: 1)
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crystal violet staining - (reply: 6)
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non-specific staining using with Immunos - (reply: 3)
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CRYSTAL VIOLET STAINING - (reply: 3)
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can coommassie stained gel be western blot - (reply: 5)
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good humidified chambers for slide staining - (reply: 2)
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RNA in situ hybridization: pH of AP staining buffer? - (reply: 1)
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Phalloidin-Alexa Fluor 488 staining problem (huge background) - (reply: 1)
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Cell staining for fluorometric assay - (reply: 5)
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Ponceau on PVDF membranes affecting immunoprecipitation antibody staining?? - (reply: 3)
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staining artifacts in polyacrylamide gel ?? - (reply: 2)
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SDS-PAGE problem with staining yellow background - (reply: 3)
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What colour do sem*n stains glow under a black light? - (reply: 1)
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SDS-PAGE staining for digestion assay - (reply: 1)
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FACS staining of human macrophages derived from PBMCs - (reply: 3)
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quantify immunofluorescence staining - (reply: 1)
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PI stain for cell viability~~Compensation? Suggestion needed~~^^ - (reply: 5)
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violet laser (405nm) and cell cycle staining with DAPI - (reply: 1)
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DiI staining Fixation protocol - (reply: 1)
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Immunofluorescence Staining of Cells - (reply: 1)
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Immunofluorescence - unspecific staining - (reply: 9)
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Oil red O staining?? - (reply: 1)
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Nuclei staining background in collagen matrix - (reply: 1)
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Sphere staining protocol (agarose) - (reply: 3)
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DAB staining and GFP - (reply: 1)
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Actin staining - (reply: 1)
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Propidium iodide staining - (reply: 1)
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Trypan Blue Staining - (reply: 3)
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problem in making a proper smear for nile blue staining of an unknown yeast - (reply: 2)
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Redoing only the Secondary Antibody staining on a Western - (reply: 1)
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RNA extraction from blood stain - (reply: 1)
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Possible non-specific staining of ribo-probe in Fluorescence in situ hybridisati - (reply: 1)
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Searching for a live cell staining method - (reply: 1)
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ANNEXIN V staining for FLOW - (reply: 3)
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What positive control for intracellular staining? - (reply: 1)
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Simultaneous Double Staining in paraffin tissues - (reply: 2)
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Which stain is frequently used to identify Mycobacterium - (reply: 1)
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Forgetting iodine when gram staining - (reply: 1)
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intracellular cytokine staining - (reply: 1)
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staining at 37 degrees or RT - OK or really bad ? - (reply: 3)
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X-gal staining and IHC against b-gal - (reply: 7)
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mass compatible silver staining - (reply: 1)
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Staining Cells in Suspension - (reply: 4)
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Bradford reagent for staining gels? - (reply: 1)
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Single stain control for 293 cell line - (reply: 4)
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Use of colloidal coomassie stained to western blotting?? - (reply: 5)
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Intracellular staining for IL-21 - (reply: 1)
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Annexin-V staining - (reply: 1)
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apoptosis assay - immunofluoresence staining (reply: 5)
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Pre-cutting before staining - How long before staining can samples be cut? (reply: 1)
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silver staining of ip sample - (reply: 1)
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immunocytochemistry - sequence of: Fix/Perm/Stain - Cytocentrifugation (reply: 1)
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GFP cells intracellular staining - (reply: 1)
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gelatin staining with coomassie: how do I make it work? - (reply: 3)
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X-gal stained tissue for IFC? - Will this work? (reply: 2)
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ICC: exotic problem with staining, vGlut1 ans synapsin-1 - (reply: 2)
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NuPAGE Gel get smaller after staining o/n - (reply: 1)
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Detection of Apoptosis by Annexin V PI staining - (reply: 1)
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Problems about detection of apoptotic cells using Annexin V/PI staining - (reply: 2)
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Histochemical stain specific for transthyretin (prealbumin)??! - Anyone aware if there s such a thing? (reply: 2)
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Fixing cells before staining of surface markers - (reply: 1)
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TLC Visualizing Stains - (reply: 1)
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Apoptosis vs Viable Cells vs Necrosis (Annexin/PI Staining) - Which quadrants to sum up? (reply: 4)
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Staining corneal endothelial cells for mechanical damage with methylen blue - (reply: 1)
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cell loss during antibody staining for flow cytometry - FACS staining protocol (reply: 5)
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Negative control is positive for IF staining - (reply: 3)
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intracelluar staining without killing cells - (reply: 3)
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Hoechst Stain does it stain dead cells - (reply: 3)
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Diastase or Amylase Digestion - Negative for PAS stain (reply: 4)
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detection of mycoplasma with hoechst staining - (reply: 1)
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short question - FDG staining (reply: 2)
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Flow cytometry-frozen PBMCs - Surface and intracellular staining (reply: 5)
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Basic questions about PI staining in Flowcyto - (reply: 7)
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T helper differentiation and staining - Trouble with intracellular staining (reply: 6)
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Help Stained after blocking and won't unstain!!! - (reply: 1)
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immunochemical staining problem - i'm struggling to resolve this problem (reply: 1)
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Help-IHC nuclear staining - (reply: 2)
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imunofluerecence staining with Alexa 568 dye - interesting (reply: 1)
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No protein spots in 2D gel - Silver staining! - (reply: 17)
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RNA extraction from stained cells - (reply: 2)
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Ponceau Red Staining Problem - (reply: 2)
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How to stain for oxidative stress - (reply: 1)
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Roles of staining soluton components!!! - (reply: 6)
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Toluidine blue staining for cell culture - (reply: 1)
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Storage of Fixed Cells for Immunofluorescence staining - (reply: 1)
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Horns like appearance on SDS-PAGE Silver stained Gel - (reply: 7)
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Annexin and propidium staining - (reply: 1)
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GUS histochemistry assay - No staining present (reply: 1)
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Good Cell line for Histone 3 and 4 - Positive Control for Basic Staining and WBs (reply: 1)
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how to stain suspension culture? - (reply: 1)
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silver stain of immunoprecipitate - (reply: 2)
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NK cell staining - (reply: 4)
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Disposal of coomassie stain - (reply: 3)
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flagella stain - (reply: 1)
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Silver stained 2-d gel BLOB!!! - (reply: 1)
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microglia staining - protocol for microglia staining (reply: 1)
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Protein Transfer Problems from SDS-PAGE Gels - Involves Coomassie Staining (reply: 15)
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what is the measurement of Oil red O staining - (reply: 3)
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Surface Staining and Methanol Fixation - (reply: 1)
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Live stain with propidium iodide - Incubation time & temperature? (reply: 3)
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immunofluorescence staining of sperm cells - (reply: 2)
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pre stained marker issues - (reply: 2)
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How to remove urea from PAA gel? - RNA silver staining (reply: 1)
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Preparation of destain solution of the Pro-Q-Diamond phosphoprotein stain - (reply: 1)
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IHC staining of secreted proteins - (reply: 3)
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strange band in coomassie blue staining - (reply: 3)
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FBS Annexin staining - (reply: 1)
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siRNA staining? - (reply: 1)
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SDS-PAGE gel staining problem - (reply: 1)
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High background in phalloidin staining - (reply: 2)
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histological staining for plant cell wall components - (reply: 1)
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Antibody staining - (reply: 6)
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precipitation of proteins from stained cells - Co-IP failure (reply: 6)
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Pre stained markers - (reply: 4)
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Staining spores - (reply: 1)
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DAPI/Hoechst vital staining - (reply: 3)
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Only borders stained in Silver!!! - (reply: 7)
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co staining problem: same primary species - (reply: 7)
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viability test: fixation AFTER propidium iodide staining? - (reply: 1)
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MDCK cells vacuolation - Vacuolation of MDCK cells while staining (reply: 4)
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Staining of GLycoproteins in IEF - (reply: 4)
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densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)
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collagen staining in cell culture - (reply: 5)
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CFSE staining of mycobacteria - (reply: 1)
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RNA elution after stains all staining - (reply: 3)
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Get rid of particles on gel surface after SYPRO-Ruby staining - (reply: 1)
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Help needed with IF staining. - (reply: 2)
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vacuolation in MDCK cells - Vacuolation after staining (reply: 4)
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PFA Quenching in Staining of 2D and 3D MDCK Cultures - (reply: 1)
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Staining of Intracellular Cytokines - (reply: 6)
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Flow cytometry CD14 staining very dim - Bone marrow cells CD14 staining for flow cytometry (reply: 3)
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Th17 Differentiation and Staining - (reply: 18)
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Annexin V staining in combination with other antibodies - (reply: 4)
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DAB Peroxidase staining - (reply: 3)
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Fixation after or before staining? - (reply: 3)
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Preabsorption of Ab with Ag still leads to staining in ICC - (reply: 7)
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Variable expression of antigen in various subcellular locations - Any ideas why? - ICC Staining on Glioma Cells (reply: 2)
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cell cycle analysis PI staining shift - (reply: 1)
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Ponceau Staining Question - (reply: 1)
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Intracellular stain of nuclear receptor - (reply: 6)
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sox-2 staining - (reply: 2)
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CFSE staining protocol - (reply: 14)
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What stains red in trypan blue - (reply: 2)
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PKH26 staining mesenchymal stem cells - toxicity (reply: 1)
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Beta Catenin immunofluorescent staining - (reply: 1)
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smeared silver stainning on acidic native page - how can I sovle smeared silver staining? (reply: 3)
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to increase the sensitivity of SDS-PAGE silver staining?? - (reply: 1)
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YFP staining - (reply: 2)
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Problem with double staining for FACS - (reply: 1)
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Ponceau staining after primary and secondary? - (reply: 5)
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staining cells - (reply: 3)
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Protocol for staining live cells - Staining live cells for Flow (reply: 2)
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Nucleic acid staining and PCR - Query onthe effect of nucleic acid stains on PCR (reply: 1)
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Purpose of methanol in the Blue Silver stain - (reply: 3)
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Bacterial Staining and Counting - (reply: 1)
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DNA vs Protein Stains - Are there any that don't stain BOTH? (reply: 1)
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different staining intensity - fixed permeabilized cells and non-fixed cells (reply: 2)
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types of gel stains - (reply: 7)
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Etbr staining methods - (reply: 6)
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Trypan Blue and Hoechst staining.. - am I doing it right?? please help.... (reply: 1)
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surface staining of THP-1 cell - (reply: 2)
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How to fix GFP expressing cell to preserve GFP signal in immunofluorescent stain - (reply: 3)
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ER stained with Ab looks bad... - (reply: 3)
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HUVEC surface antigen staining - (reply: 1)
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Phosphoproteins staining - (reply: 2)
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apoptotic bodies- DAPI staining - (reply: 6)
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Integrin ß1 staining - (reply: 2)
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Staining for neural crest derived cells?? - (reply: 2)
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problem to stain just one cell line in coculture - (reply: 4)
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ATPase staining for different Myosins - Anyone got a good protocol? (reply: 3)
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Is immunoblotting possible after the gel has been silver stained - (reply: 2)