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Protein Related Discussions
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epitope N1 peptide SARS nucleocapside - (reply: 4)
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Resuspending protein mixture after over-centrifuging - (reply: 1)
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Time course experiment on protein level - (reply: 5)
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TA cloning for sars cov 2 nucleocapsid protein. - (reply: 4)
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Liquid nitrogen protein extraction ?. - (reply: 2)
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Protein concentration for ext. coefficient - (reply: 4)
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Protein identification - (reply: 1)
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Standard HCL protein hydrolysis technique without vacuum - (reply: 1)
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Virus vs protein purification - (reply: 2)
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How to eliminate inherent proteins contamination during protein purification? - (reply: 1)
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Cloning protein of interest into lentiviral vector - help - I have no experience - (reply: 20)
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?what is better for protein precipitation ammonium acetate or sodium chloride in - (reply: 3)
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ebv igm borderline - (reply: 1)
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Electrophoresis of proteins from cells - (reply: 9)
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Protein purification questions - (reply: 3)
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A single point mutation enhanced protein expression but attenuated its function - (reply: 4)
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S. cerevisiae protein secretion levels (mass) in minimal media - (reply: 1)
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How to obtain a non-cellular protein in its natural form? - (reply: 4)
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Fixation for surface epitope detection - (reply: 1)
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Blunt-end ligation: what if the vector and insert are both phosphorylated? - (reply: 4)
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Normalise cell count for protein analysis - (reply: 3)
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Protein expression issue - (reply: 4)
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Problem with protein induction - (reply: 2)
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Western-No bands for sample protein but loading control works - (reply: 5)
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SDS-PAGE/2D gel and western blot for multiple proteins for multiple sample - (reply: 1)
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Percentage of Outer Membrane Protein - (reply: 2)
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ubiquitinylated proteins - (reply: 6)
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Storage period of protein - (reply: 1)
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Outer membrane protein extraction - (reply: 1)
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HEPES BUFFER and protein degradation - (reply: 1)
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Outer membrane protein extraction - (reply: 5)
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Virus vs protein purification - (reply: 3)
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Amount of protein - (reply: 1)
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how to predict transmembrane protein structure - (reply: 7)
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How to add prepare protein sample for loading into the gel? - (reply: 3)
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How to make antibody based affinity column for protein purification - (reply: 4)
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Express two different membrane proteins on the same promoter - (reply: 1)
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Problem with Rosetta gami B protein expression!!!! - (reply: 1)
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bacterial surface protein extraction - (reply: 1)
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determination of protein concentation - (reply: 1)
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Protein concentration in mM - (reply: 3)
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protein concentration in molar amount - (reply: 1)
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Low weight protein degradation during western blot - (reply: 1)
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Calculation of mg/ml protein concentration in my sample - (reply: 1)
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Salting out specific protein - (reply: 1)
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protein purification from agarose beads using immunoprecipation kit - (reply: 2)
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Re-purification of His-tagged protein over IMAC - (reply: 2)
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IHC Protein Competition, Protein Background - (reply: 1)
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ELISA working for peptide, but not working for protein - (reply: 7)
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protein sequence mutagenesis - (reply: 2)
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Database of only characterized proteins - (reply: 3)
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Protein detection and quantitation on textiles - (reply: 1)
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Pros and Cons of refolding proteins - (reply: 3)
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Use of urea in solubilizing protein and its aftermath - (reply: 5)
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Protein quantification using HPLC/fluorescence - (reply: 3)
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GST fusion protein - two dominant bands - (reply: 7)
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Nonspecific proteins eluting with HIS-tagged peptide - (reply: 4)
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Effect on protein of low pH? - (reply: 1)
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Increasing protein solubility in general - (reply: 3)
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Help with FPLC AIEX/CIEX: Keeping E. coli Protein Separated/Buffer Selection - (reply: 7)
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Best protocol to solubilize bacterial fusion proteins? - (reply: 5)
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BCA microplate assay with 595 nm filter? - (reply: 1)
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Doubt on HisTag protein purification miniprep - (reply: 3)
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"Leaky" stepwise elution of active protein from Ion-Exchange columns - (reply: 2)
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Storing temperature for protein after SDS sample buffer and boiling? - (reply: 2)
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Stability of a typical protein at 4oC? - (reply: 1)
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Heating to denature and reduce protein: same temperature? - (reply: 1)
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troubleshooting is needed for heme protein Ni-NTA purification - (reply: 2)
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Dissolving a protein abstract - (reply: 5)
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bacterial culture volume for recombinant protein to be detected in Western Blot - (reply: 3)
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Quick and simple protocol for protein purification and extraction, thoughts? - (reply: 2)
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Non-specific binding of protein in western blot - (reply: 3)
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Protein that used to express really well stopped expressing. - (reply: 2)
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I can't believe peptide inhibition is a valid epitope mapping method - (reply: 1)
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Zeba buffer exchange low protein yield - (reply: 6)
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study protein expression in bacteria - (reply: 1)
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Quantification or Quantitation (of Nucleic acids and proteins)? - (reply: 1)
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total protein measurement from insect body - (reply: 1)
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using Pichia for commercial protein production - (reply: 1)
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industial scale protein production - (reply: 3)
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Does over-expression protein under T7 promoter in Top10 work? - (reply: 1)
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Large-scale cell growth and protein expression - (reply: 1)
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Is there a way to label a protein translated from a known in vitro-transcribed m - (reply: 1)
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why a protein domain fold independently? - (reply: 1)
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mRNA vs protein correlation issues, breaks central dogma - (reply: 1)
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Liquid nitrogen protein extraction - (reply: 3)
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protein extraction from lettuce - (reply: 1)
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Reduce protein concentration without reduced enzyme activity - (reply: 3)
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consistent expression of overlarge proteins - (reply: 1)
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Protein Clevage - (reply: 1)
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Immunostaining non-hisstones protein in histone extracts - (reply: 3)
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Is AS pp + HIC phisically possible for all proteins? - (reply: 2)
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storage conditions for purified protein - (reply: 2)
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Protein expression - (reply: 1)
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Concentration of Sample Buffer to boil protein samples - (reply: 2)
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Protein denaturation - (reply: 5)
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Human cell membrane protein Western blot! - (reply: 2)
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Protein concetation at 280 nm - (reply: 1)
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lysate protein concentration - (reply: 1)
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protein staining with EtBr!! - (reply: 4)
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large scale protein purification - (reply: 6)
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Insoluble protein after ammonium sulfate precipitation - (reply: 1)
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Question regarding protein conservation among similar genes - (reply: 2)
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downregulation of protein with ON-TARGET plus Non-targeting pool - Dharmacon - (reply: 1)
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recombinant protein expression- surface or internal? - (reply: 3)
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His-tag protein not binding to resin - (reply: 9)
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Akt phosphorylation at Thr308 and Ser473 - (reply: 1)
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Docking of a protein with glycolipid receptor - (reply: 2)
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Protein localisation - IHC - (reply: 1)
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protein size in SDS-PAGE - (reply: 4)
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Protein not migrating through gel - (reply: 9)
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Visualizing exons and their respective transcripts and protein domains. - (reply: 3)
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Reconstitution of a hygroscopic protein. - (reply: 2)
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protein extinction coefficient - (reply: 1)
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Bradford positive but no Protein on SDS PAGE - (reply: 4)
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how to find Transmembrane domains in Proteins ?? - (reply: 2)
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IgG control for IP - (reply: 1)
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Protein stuck in the origin of resolving gel - (reply: 12)
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RECOMBINANT PROTEIN - (reply: 2)
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Loss of protein expression by transformed bacteria - (reply: 4)
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37kb and 7kb protein in western blot - (reply: 24)
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Cell lysis method to get "intact" nuclear protein complex for CO-IP? - (reply: 1)
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detection of a protein band of protein complex - (reply: 3)
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Protein expression database - (reply: 3)
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protein modification - (reply: 1)
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protein interaction service - (reply: 2)
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after protein purification - (reply: 4)
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SDS-PAGE protein analysis with antibodies alone (not WB) - (reply: 1)
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What post-translational modifications help proteins migrate faster on an SDS-PAG - (reply: 8)
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What is best to measure protein expression: western blot or ELISA? - (reply: 4)
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How do I know the protein structure? - (reply: 3)
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Western blot detects exogenous protein but not endogenous - (reply: 2)
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recombinant protein vs native protein - (reply: 1)
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Question with the molecular weight of my protein - (reply: 1)
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protein expression site - (reply: 3)
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Possible Protein Aggregation Riddle - (reply: 3)
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Insert phosphorylation or digestion - which should be done first? - (reply: 4)
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Looking for a florescent protein with an intron - (reply: 1)
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Pull-Down Assay, EMSA Assay: recombinant protein vs nuclear extracts - (reply: 5)
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Expression of plant protein in Ecoli - (reply: 1)
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Mystery of SDS gel, working with the protein GB1 (T2Q mutation) - (reply: 3)
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Protein Expresion and Isolation from Lactobacillus - Insoluble protein - (reply: 1)
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protein expression - (reply: 2)
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Lack of reproducibility using BCA to quantitate total protein - (reply: 5)
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Problem with Western Blotting - BCA assay shows protein but no protein bands whe - (reply: 3)
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Trouble eluting all protein from avidin beads - (reply: 1)
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Protein knockdown method? - (reply: 1)
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How do I extract soluble protein after adding SDS-PAGE buffer? - (reply: 1)
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Oxidative phosphorylation - (reply: 3)
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Discovering protein–lncRNA interactions from large-scale CLIP-Seq and RNA-Seq da - (reply: 1)
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Best epitope for IF with a poor-expresser - (reply: 2)
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How to find the interaction between two proteins - (reply: 2)
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Collagen binding protein expression E. coli His-tag - (reply: 1)
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Lysis buffer for proteins - (reply: 6)
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Elute fractions that have target protein in FPLC is unstable - (reply: 8)
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Protein ID in ESI QTOF - Protocol - (reply: 1)
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Large protein removal (fractionation) - (reply: 6)
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Can I loose the protein of interest from a stable clone? - (reply: 2)
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unexpected protein bands - (reply: 2)
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Detection of Td-Tomato protein in frozen sections - (reply: 2)
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Quantification of IgG - (reply: 3)
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Non-pH-sensitive fluorophore for relative protein quantification - (reply: 1)
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What is the best antibody for the housekeeping protein (control) in skeletal mus - (reply: 3)
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IgG/IgM levels in infections/Brucellosis - (reply: 1)
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No protein on western blot but standard transferred - (reply: 2)
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Protein bands visible on unstained SDS-PAGE - (reply: 1)
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trypsin digestion of 2D-PAGE protein spot - (reply: 2)
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Validating Recombinant Protein - (reply: 1)
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Protein active site size calculation - (reply: 1)
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several bands in non-reducing SDS-page gel - protein without cysteines - (reply: 2)
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protein G - (reply: 1)
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Question: Removal of Bacteria from Protein to achieve 100cfu/gm - (reply: 5)
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Western blotting - methods to detect two proteins of a similar size? - (reply: 2)
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the same construct, two stable cell clones with different protein size - (reply: 2)
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Western Blot: protein band did not appeared at the correct location - (reply: 3)
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I want to separate two proteins having mw of 16.8 and 16.78 kDa. how can i accom - (reply: 2)
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epitope tagging of protein - (reply: 1)
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Finding potential binding targets for a transcriptional regulator protein? - (reply: 1)
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i am trying to get rid of unexpected protein band by centricon - (reply: 3)
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Choosing MWCO For Dialysis Membrane - (reply: 3)
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Making a fusion protein - (reply: 1)
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CO- IP for nuclear protein - (reply: 4)
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Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
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Resuspension of Protein after TCA Precipitation - (reply: 1)
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Almost (?) accidental use of 10X transfer buffer for 350-kDa protein - (reply: 4)
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how to interpret or present elisa result for IgA in pig obtained from 120 saliva - (reply: 3)
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Ladder and proteins do not migrate into stacking gel - (reply: 5)
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Trichloroacetic Acid precipitation of proteins - (reply: 2)
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How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
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How to increase protein expression from in vitro transcribed mRNA - (reply: 1)
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Protein degradation SDS PAGE - (reply: 5)
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Protein extraction from RNAlater - (reply: 1)
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protein expression with and without induction - (reply: 1)
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Puromycin resistance protein expression and stability - (reply: 5)
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Prion protein - (reply: 3)
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Please help me :Protein expression and purification - (reply: 1)
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Calculating molar ratio & dilution of protein cluster solution - (reply: 9)
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Viral Proteins Are Not Migrating Down the Gel - (reply: 10)
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genotype of e coli strain for protein expression - (reply: 2)
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Blocking agent in western blotting of histone proteins - (reply: 1)
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Problems with detection of protein expressed in yeast - (reply: 1)
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How do I go about making a set of protein standards for protein quantification v - (reply: 2)
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Protein Refolding - Precipitation during dialysis - (reply: 3)
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What is the RNA:Protein ratio for ONE gene over different RNA levels? - (reply: 1)
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Protein Glycosylation and SDS-PAGE - (reply: 2)
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Using a mouse antibody for IP - should I use Dynabeads with Protein G or with sh - (reply: 1)
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protein turn-over - (reply: 8)
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I can detect my Flag-HA tagged protein with anti-HA but not anti-Flag - (reply: 4)
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protein structure visualisation - (reply: 3)
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heme protein precipitation - (reply: 4)
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how much purify protein nedded for IP - (reply: 9)
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lyophilized protein dilution - (reply: 6)
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Protein isolation for tissue stored in RNAlater - (reply: 1)
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regarding protein purification using 8m urea and sds page - (reply: 2)
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Question about IgG & IgM cross reactivity - (reply: 1)
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detection of metal in protein - (reply: 4)
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Do you go by 'total protein' or 'tissue weight'? - (reply: 1)
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Protein Purification from mammalian cells - (reply: 1)
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Non-denaturing buffer to extract nuclear protein - (reply: 1)
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Immunofluorescence for detecting proteins in cytoplasm - (reply: 6)
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How many ways protein protein interactions are regulated? - (reply: 1)
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Protein quantification after TRIzol Extraction - (reply: 2)
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Protein fragment is alwasy degraded post transfection 48 hrs - (reply: 2)
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Can an protein be tolerized against if it doesn't have an MHCII epitope? - (reply: 1)
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in what technique can i use to detect specific proteins - (reply: 5)
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Modeling of a protein with disulfide bond - (reply: 7)
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Protein is present, but not detected by antibody - (reply: 4)
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fluorescent tag on membrane protein - (reply: 2)
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Is it possible to attach any protein with GFP and express together? - (reply: 6)
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loading control issue in blot with H9c2 cell protein, pleeeeeasee help - (reply: 2)
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Have I used too much protein in my ELISA? - (reply: 1)
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High MW IgG signals in IP/co-IP with protein G - (reply: 1)
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Pulldown of polyubiquitinated proteins - (reply: 1)
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PCR that leads to protein synthesis - (reply: 18)
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membrane protein expression - (reply: 2)
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purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
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structure prediction of a Protein with disulfide bond? - (reply: 4)
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Ligand-controllable degron (Shield-1) for tunable protein stability - (reply: 1)
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identification of major protein; public 2D/mass-spec databases? - (reply: 2)
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having problems with p-erk antibody and other phosphoproteins antibodies - (reply: 1)
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SNP effect on protein binding affinity - (reply: 5)
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Help, protein ladder didn`t separate - (reply: 3)
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Protein concentration and extinction coefficient - (reply: 2)
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N-terminal epitope tag - keep or remove ORF start codon - (reply: 1)
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Protein secretion wtih pFuse expression system in 293T cells - (reply: 2)
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Which epitope tag to use for ChIP? - (reply: 1)
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Total Protein in COS-7 Cells - (reply: 1)
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protein extraction from cell nucleus - (reply: 2)
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Determining phosphorylation status of a protein by transfection - (reply: 5)
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Need to strip the membran before probe the internal control protein - (reply: 3)
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IgG purification from hybridomas - (reply: 2)
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Low molecular weight proteins below 30kDa - (reply: 3)
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Is NuPAGE Bis-Tris gel for native or denaturing protein? - (reply: 1)
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Separation of glycerol from protein - (reply: 6)
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Is Protein A present on the Staphylococcus saprophyticus cell wall too? - (reply: 2)
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How does the first ribosomal protein get synthesized? - (reply: 2)
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Protein bandshift in western blot of cell lysate vs immunoprecipitation eluent - (reply: 4)
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transformation and protein purification calculation help; very basic - (reply: 5)
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relationship between protein size, lysate concentration, and band intensity - (reply: 3)
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cytoplasmic protein - (reply: 1)
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B Per for total bacterial protein extraction - (reply: 2)
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transfer of low molecular weight protein - (reply: 3)
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phospho-protein and total protein significance - (reply: 1)
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Sudden problems with Separating Proteins using SDS Gel - (reply: 4)
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Rate of protein production with pcDNA 3.3 transfected into HEK293 - (reply: 3)
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no protein expression - (reply: 3)
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software to predict domains to proteins interactions - (reply: 5)
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protein half-life assesment - (reply: 3)
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Do protein purification under partially denatured condition - (reply: 1)
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Discoloration of Nickel affinity resins during 6-his protein purification - (reply: 1)
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Protein precipitation by storage - (reply: 11)
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designing fusion protein with Flag tag - (reply: 1)
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Problems with biotynylated protein binding to monomeric avidin - (reply: 1)
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Control protein for eukaryotic expression - (reply: 5)
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Protein precipitation method - (reply: 2)
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Protein extracted from RNAlater tissues is blue... Help! - (reply: 2)
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Flag fusion protein not detected by Flag antibody - (reply: 8)
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Protein repeatedly does not express from pMX retroviral vector-advice needed des - (reply: 10)
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No reduction in protein expr after 2nd passage of transfected cells - (reply: 3)
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what are albumin associated proteins ?and can you please name them? - (reply: 1)
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Elution of biotonylated proteins - (reply: 1)
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Which domains are commonly found to mediate protein protein interaction? - (reply: 1)
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instability index of expressed protein - (reply: 1)
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Protein Purification-catastrophe while purifying? - (reply: 2)
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differentiating proteins in/on bacteria - (reply: 1)
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Protein purification for adherent mammalian cells - (reply: 2)
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Zero absorbance by Bradford assay for Dialyzed protein - (reply: 18)
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SDS and urea lysis, protein degradation and freeze-thaw cycles - (reply: 4)
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Prey protein band bleeds, IgG band normal? - (reply: 3)
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Phosphorylation of recombinant proteins in E. coli - (reply: 1)
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No proteins after HeLa cell lysis - (reply: 3)
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What is Role of HA in the biology of influenza virus and how this protein influe - (reply: 1)
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Malachite Green ATPase Assay - How to deactivate the protein and what is the sen - (reply: 1)
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Protein expression - which is more sensitive/accurate? - (reply: 1)
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half life of a protein - (reply: 3)
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Can protein isomers be detected in SDS-PAGE - (reply: 3)
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Blocking a protein in cell culture through antibody - (reply: 10)
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Luciferase fusion protein? - (reply: 1)
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NusA tag produces protein truncations? - (reply: 3)
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Probing protein - (reply: 1)
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Tissue-Specific Protein Expression Database - (reply: 4)
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can anyone help me with calculating a molar ratio of protein to lipid? - (reply: 4)
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Problem with strep tagged protein purification - (reply: 5)
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production of phosphorylated proteins in E.coli host system - (reply: 1)
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why i get double band for recombinant protein - (reply: 9)
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What is the reason of doing proteomics work? - (reply: 2)
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Protein quantification - (reply: 4)
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Protein extraction for 2D electrophoresis - (reply: 2)
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Protein extraction buffer preparation (please check for accuracy) - (reply: 6)
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Test for protein in lysate - (reply: 4)
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How to dissolve TCA/acetone precipitated proteins? - (reply: 1)
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protein purification in acanthamoeba lysate - (reply: 3)
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LMW proteins not transfer of gel to membrane - (reply: 1)
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Why different elution buffers for Protein G and Protein A chromatography? - (reply: 1)
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screening Protein Expression - (reply: 1)
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Insufficient Transfer of proteins through semi dry transfer - (reply: 6)
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How many PBMCs should I seed so that I can get enough protein? - (reply: 1)
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co-ip of endogenous and tagged proteins - (reply: 1)
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purify protein from native gel - (reply: 1)
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purifying proteins with repeats - (reply: 3)
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Protein purification from greenish supernatant of Pichia pastoris - (reply: 10)
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BCA assay - (reply: 1)
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Elusive protein refuses to be found in human serum with ELISA - (reply: 3)
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SDS-PAGE abnormalities in proteins near to front dye - (reply: 5)
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Membrane protein - (reply: 1)
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adding Tween80 to bacterial culture for protein purification ?! - (reply: 3)
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Protein sequence conservation - (reply: 2)
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MALDI MS resutls to identify proteins - (reply: 2)
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Wrong protein size on western blot - (reply: 10)
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Protein measurement of Laemmli samples - (reply: 2)
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Anyone has experience in using estrogen receptor (ER) fusion protein? - (reply: 1)
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Difficulty in Expression of Protein Having 73% GC contents in GST Tag Vectrors - (reply: 3)
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Is there a such thing that would allow me to generate 2 individual proteins, but - (reply: 5)
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SDS Page Problem: Proteins stuck at stacking line - (reply: 3)
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how to detect the modification like phosphorylation on one amino acid locus of o - (reply: 2)
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GST pull down assay: GST binds to the prey protein. Negative control shows bindi - (reply: 4)
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Problem in acetone precipitation of plant proteins. - (reply: 1)
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Trouble with purified recombinant protein bands - (reply: 3)
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Would IP make the protein of interest run slower on SDS-PAGE? - (reply: 2)
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Extraction of Transmembrane Proteins - (reply: 1)
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His Tagged Recombinant Protein not Recognized in Western Blot - (reply: 1)
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Protein folding in different species - (reply: 1)
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Problems in expression of target protein in E. coli - (reply: 3)
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Protein structure modeling for enzymes with point mutations - (reply: 3)
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Membrane protein extraction - (reply: 2)
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Protocol for detecting soluble, secreted proteins on a Western? - (reply: 1)
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Protein activation after purification - (reply: 4)
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Protein amount to load for publication figure? - (reply: 4)
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Identifying new Ca2+-binding proteins - (reply: 7)
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Membrane Protein Extraction - (reply: 2)
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protein fractionation - (reply: 4)
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Native gel electrophoresis: Can it tell if a protein is forming dimer? - (reply: 1)
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What is different between Native protein and Denatured protein samples. - (reply: 4)
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Insoluble Protein of Interest Resulted from Mammalian Gene Expression - Can Any - (reply: 3)
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Isolation of protein and RNA from RNeasy Minikits? - (reply: 5)
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IgG heavy chain from IP - (reply: 8)
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protein extraction and peptide quantification from fat breast tissue - (reply: 2)
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experiments to determine the relationship between proteins - (reply: 4)
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trouble in his-tagged protein binding with Ni-NTA agarose - (reply: 3)
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Contaminants in protein purification.....help! - (reply: 3)
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Western Blotting loading control for large proteins - (reply: 2)
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Protein expression and purification - Please help - (reply: 2)
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Protein purifcation without chromatography - (reply: 6)
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Protein of 460 kD on western blot - (reply: 3)
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tag for protein purification - (reply: 7)
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Quantitation of protein concentration in rehydration buffer - (reply: 3)
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Protein is not Binding to DE52 column material - (reply: 2)
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Are two promoters needed for co-expressing 2 proteins from the same plasmid in S - (reply: 4)
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membrane proteomics - (reply: 1)
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Membrane proteins on 2D gel electrophoresis..... - (reply: 1)
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MBP-Tagged protein degrading after expression - (reply: 1)
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How to estimate the protein expression level from the SDS_PAGE? - (reply: 8)
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Protein quantification using HPLC - (reply: 1)
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Western Quantification (No BCA???) - (reply: 2)
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Protein extraction and isolectric focusing - (reply: 1)
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Western Blot: only non-specific bands and not protein of interest - (reply: 3)
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Inhibition of a protein upstream in signaling cascade ? - (reply: 5)
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fast human protein function catalog? - (reply: 3)
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Protein puriification using IMAC: flowthrough very slow - (reply: 2)
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Sediments/debris/pellet in protein lysate? - (reply: 3)
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Trouble in GST- fusion protein purification - (reply: 1)
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Helppurification of expressed protein - (reply: 2)
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Protein ladder not separating - (reply: 6)
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protein expression by changing start codon - (reply: 1)
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low concentration protein purification - (reply: 1)
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Western Blot - Protein Marker Present on Film After Developing - (reply: 1)
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Host cell protein sequence search - (reply: 1)
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(best cell lysis method for) protein expression analysis by western blot - (reply: 2)
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Using centrifugal filter units to concentrate protein - (reply: 10)
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Protein isolation and lack of sop's - (reply: 1)
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membrane proteins ultrafiltration - (reply: 2)
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IgG control Immunofluorescence : have problem - (reply: 9)
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Determining protein concentration after solubilisation in Triton X-100 - (reply: 2)
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Purification of RNA binding proteins by avidin agarose beads - (reply: 2)
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specific phosphorylation - (reply: 2)
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Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
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Conversion of protein from kDa to gram/L - (reply: 4)
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Reference protein give different expression - (reply: 3)
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how to convert RFU/sec of my Vmax in mol/min/mg of protein?? - (reply: 4)
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recombinant protein form - (reply: 2)
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EMSA: cell extract vs purified protein - (reply: 2)
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Low Protein Content In Cell Lysate - (reply: 1)
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Membrane protein concentration - (reply: 1)
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get EST/ cDNA accession number for protein - (reply: 1)
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Western blot question- High % Gel/Low MW Protein - (reply: 7)
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Protein expression opposite of mRNA expression. Ideas? - (reply: 6)
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tagged protein size - (reply: 10)
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Protein concentration of a labelled membrane protein - (reply: 2)
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WB of cellular proteins in human serum - (reply: 2)
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Protein purification method to isolate elicitor with biological activity - (reply: 1)
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Protein expression in e.coli - (reply: 5)
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problem in extracting the protein - (reply: 1)
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purification and binding of protein in denaturing conditions - (reply: 2)
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BCA Protein Assay and ELISA afterwards - (reply: 1)
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crosslinking of protein and generate antibody - (reply: 2)
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Protein purification problems: Presence of contaminated proteins along with the - (reply: 5)
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protein sample floating out of the tricine gel - (reply: 3)
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minimum recombinant protein amounts for gst pulldown - (reply: 4)
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Protein Purification from Polyacrylamide Gels - (reply: 3)
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Protein Expression vector with small tag - (reply: 1)
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Protein precipitated while kept at 4C over night after elution from a Ni-NTA col - (reply: 4)
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Protocol for elution of desthiobiotin-labelled proteins from Streptavidin Column - (reply: 2)
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Software for scanning of specific aa sequence in proteins - (reply: 5)
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Membrane Protein SDS PAGE and WB - (reply: 1)
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Wet transfer of proteins between 100-200kDa - (reply: 2)
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Formula of crude protein analysis - (reply: 1)
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Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
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protein concentration and Pull down - (reply: 3)
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High Yield Protein Expression 10g/L - (reply: 5)
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No activity of expressed protein obtained from pET32a cloned gene - (reply: 6)
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GST- tagged protein expressed in insoluble - (reply: 5)
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Purifying a "protein" - (reply: 2)
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Antibodies against protein complex - (reply: 4)
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converting protein names protein ids and enterz gene ids - (reply: 1)
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SDS solubilisation, Membrane Proteins and Micelle Formation - (reply: 2)
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Prepare protein standards for concentration determination - (reply: 1)
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Protein degradation on 2D gels - (reply: 2)
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E-book on recombinant protein expression - (reply: 2)
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small proteins - (reply: 2)
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protein Protein Linking - (reply: 5)
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Protein and RNA interaction - (reply: 1)
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SDS PAGE - Band artifacts when protein buffer is pH 5.0 and heated - (reply: 2)
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Immunoprecipitated protein visible on coomassie but not detected by Western - (reply: 4)
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dialyse of a protein - (reply: 4)
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Elimination of residual protein in cytosol - (reply: 2)
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Trouble estimating my protein size using ladder (image included) - (reply: 2)
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Bradford vs Lowry protein concentration gives dramatic different results - (reply: 5)
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Eliminating Protein/Protein Interactions for Western Blot - (reply: 2)
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Protein Extraction: Extremely Sticky e coli lysate - (reply: 1)
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Inclusion body formation of the recombinant protein expressed in pET28a in E.col - (reply: 3)
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Is there any software that can predict DNA binding proteins based on input DNA s - (reply: 2)
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Tagged recombinant protein has different mobility? - (reply: 7)
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How to stabilized a recombinant protein - (reply: 1)
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Total protein determination before Elisa? - (reply: 2)
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getting negative protein measurements - (reply: 3)
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Mass-spectrometry of membrane proteins - (reply: 1)
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CCRK (CDK20) protein expression and western blot - (reply: 7)
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Human IgG Elisa variability problems - (reply: 2)
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My protein doesn't bind to the Ni-NTA column - (reply: 11)
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Phosphotidyl ethanolamine conjugated protein - (reply: 1)
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TFE in inducing alpha-helix formation in proteins - (reply: 3)
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Quantify Chromatin With Protein Assay? - (reply: 1)
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Acetone Precipitation of proteins - (reply: 1)
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Mouse IgG detection - Blocking ? - (reply: 2)
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TCA IgG precipitation - (reply: 2)
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How to load equal amount of protein without using spectramax - (reply: 4)
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metal chelation for proteins - (reply: 3)
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protein not stained by Coomassie Blue - (reply: 13)
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Protein stability at pH8.3 - (reply: 2)
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No resolving of protein/bands below 50kDa :-s - (reply: 5)
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Designing truncated protein as antigen - (reply: 2)
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protein is not expessing in HEK 293 cells - (reply: 2)
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Transferring >150 kDA proteins conditions - (reply: 3)
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Purification of FC tagged proteins - (reply: 3)
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Brief vortexing for reconstitution of recombinant proteins? - (reply: 1)
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question about protein expression normalization. - (reply: 6)
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protein induction from yeast - (reply: 1)
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mass spectrometry and tryptic digestion on membrane proteins - (reply: 5)
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In vitro transcription/translated protein to cells!!! - (reply: 1)
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Expressing protein in non-E.coli - (reply: 2)
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Protein bands not migrating - (reply: 3)
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Tumor protein precipitation at denaturing step? - (reply: 1)
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Does triple labeled protein precipitate more easily? - (reply: 2)
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diluting standards BCA assay - (reply: 2)
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measurement of proteins in a mixture of soluble-insoluble proteins - (reply: 5)
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Role of Isoleucine in protein structure. - (reply: 1)
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Cell confluency, transfection, protein expression for 293T cells - (reply: 2)
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Large proteins in Western - (reply: 12)
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SDS-PAGE trouble with protein concentration - (reply: 2)
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Protein Size 50 kDa higher than expected - (reply: 3)
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protein extraction from human tissue (very small) to do ELISA - (reply: 5)
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Protein precipitation from RIPA buffer to perform ELISA - (reply: 4)
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Method for protein concentration measurement - (reply: 8)
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Stable protein expression in Vero cells - (reply: 2)
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Why GFP gets cutted from my fusion protein? - (reply: 9)
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Phospho-protein vs total protein Western blot - (reply: 5)
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Relationship between total protein and enzyme activity - (reply: 3)
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protein with good soulbility but doesnt bind to Ni-NTA coloumn - (reply: 4)
-
GST fusion protein purification - (reply: 2)
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Is protein degrade by UV ? - (reply: 4)
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Can I test protein denaturation after sonication - (reply: 2)
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myc-tagged proteins - (reply: 1)
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GST-tagged protein expression problem! :-( - (reply: 4)
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Uniprot protein corresponding nucleotide sequence - (reply: 1)
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Co-IP: Prey Protein Present in Neg and Pos Eluates - (reply: 5)
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How OD600 influence on IPTG induction and protein expression - (reply: 5)
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Protein extraction from mouse tissue? - (reply: 1)
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Can a anti-IgM antibody developed for BCR bind IgM - (reply: 1)
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finding the protein and nucleotide matches - (reply: 1)
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Mouse IgG1 purification - Protein G - (reply: 1)
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Overexpressed protein shows a band higher than expected?! - (reply: 2)
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Why can't we detect multiple protein in a single membrane using western blot - (reply: 2)
-
Bradford Protein Assay - Problem in BSA Standard Curve - (reply: 2)
-
Protein structure prediction - (reply: 1)
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sedimentation of cytosolic proteins - (reply: 2)
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Determining pI of a protein - (reply: 3)
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Multiple protein bands after expression - (reply: 6)
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ethanol precipitation of protein - (reply: 1)
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Could anyone help me out with the removal GST tag from my recombinant protein? - (reply: 1)
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co-eluted protein with his-tag protein - (reply: 1)
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Help with His-Tag protein purification - (reply: 2)
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Use dialysis tube to concentrate virus - (reply: 8)
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How to thoroughly solubilize proteins from tissue sample, for quantitation - (reply: 1)
-
protein showing up around 50 kda rather than 20 kda - (reply: 2)
-
Protein Concentration from Bacterial Lysates: Help! - (reply: 2)
-
Is DTT reduction and alkylation critical before concentrating proteins? - (reply: 1)
-
cloning without phosphorylation - (reply: 7)
-
Concentrating a diluted protein and protein determination - (reply: 4)
-
IHC: confirming specificity phosphorylated protein antibody - (reply: 2)
-
protein extraction from GEL - (reply: 5)
-
Decreased protein yields after successive passaging? - (reply: 1)
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Constitutive p42/44 MAPK phosphorylation - (reply: 2)
-
His Tag Cleavage (protein precipitates) - (reply: 3)
-
protein biochemistry - (reply: 1)
-
How to distinguis soluble from insoluble recombinant proteins produced in E. col - (reply: 1)
-
fusion of cDNA expressing protein with gfp - (reply: 1)
-
GFP fusion protein not expressing! - (reply: 2)
-
can Protamine sulfate inhibit or degrage a protein? - (reply: 1)
-
plant extraction procedure for protein - (reply: 3)
-
HiTrap Heparin HP for purification of DNA binding proteins. - (reply: 1)
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Transfer of proteins from gel to PVDF for short period - (reply: 3)
-
interest protein and total protein ratio range - (reply: 2)
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Can not reduce protein level - (reply: 1)
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Precipitation during dialysis - (reply: 2)
-
Cloning genetic sequences of proteins. - (reply: 1)
-
Experience with Y-PER Plus, dialyzable yeast protein extraction reagent - Pierce - (reply: 1)
-
Fold-changes different when quantifying same protein via IF and Western - (reply: 1)
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Low expression levels of highly soluble protein - why is ON expression not helpi - (reply: 8)
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target mRNA/protein levels increase with siRNA - (reply: 2)
-
How exactly do i show two proteins interacting? - (reply: 2)
-
Reversed-Phase chromatography for protein purification - (reply: 3)
-
All-encompassing proteomics workflow? - (reply: 1)
-
Western Blot Antibody Epitope - (reply: 1)
-
Easy method to lyse E. coli pellet (follow-up of GST fusion protein purification - (reply: 4)
-
problem with purification of gst-tagged protein - (reply: 2)
-
protein aggregation (?) in gelatin zymography - (reply: 1)
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Signal peptide removed when making recombinant protein - (reply: 1)
-
problem with protein identification - (reply: 8)
-
Methods of concentrating total protein extracts - (reply: 2)
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All mRNA translated into protein - (reply: 2)
-
Protein degradation during expression - (reply: 3)
-
Protein extraction - (reply: 5)
-
Precipitation Problems with Concentrating Proteins with PEG - (reply: 1)
-
Commercial standard protein ladder/marker >250? - (reply: 2)
-
protein size determining methods - (reply: 1)
-
Proteins below 60 kDa not resolving in SDS-PAGE!! - (reply: 1)
-
Immunofluorescence for detecting three proteins. - (reply: 2)
-
Best gel percentage to separate 24 and 36 kDa proteins - (reply: 3)
-
Can carbamylation of proteins take place without heating? - (reply: 1)
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How to rescue my proteins? - (reply: 2)
-
Expression of insoluble protein - (reply: 4)
-
How to get pure protein of a gene - (reply: 1)
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Homogenisation of protein - (reply: 1)
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How to get soluble protein? - (reply: 3)
-
protein concentration/well calculation - (reply: 1)
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How to get pure protein of a gene - (reply: 3)
-
study of the role of a protein in a lesion - (reply: 3)
-
IPTG induced protein go to inclusion bodies - (reply: 1)
-
'Standard salt buffer' for protein storage/biophysical techniques - (reply: 1)
-
Protein Isolation from mouse brain: problems - (reply: 2)
-
Protein extraction from dead cells - (reply: 1)
-
Problems with high molecular weight protein transfer in western blot - (reply: 1)
-
Expression level analysis at mRNA vs protein level - (reply: 6)
-
Trouble Western Blotting a 360kDa Protein - (reply: 5)
-
Purifying recombinant protein from pellet? - (reply: 2)
-
Protein ladder - (reply: 1)
-
Insoluble recombinant protein is expressed also with out IPTG - (reply: 4)
-
Question about the amount of mRNA and secreted protein level!!! THX~ - (reply: 1)
-
Problem with removing fusion protein. MALDI TOF. Tricine SDS PAGE. - (reply: 1)
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Problem with removing fusion protein. MALDI TOF. Tricine SDS PAGE. - (reply: 1)
-
membrane protein expression - (reply: 1)
-
normalization to protein content or cell number - (reply: 2)
-
protein solubilization and quantitation after extraction using Trizol - (reply: 2)
-
Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
-
Magnesium acetate in protein lysates - (reply: 1)
-
detection of phosphorylation from cell pellet - (reply: 2)
-
How to prepare Tris-HCl buffer for protein crystallization? What does 33mM mean? - (reply: 6)
-
Protein sequencing gaps - (reply: 1)
-
which protein can I use as a loading control for nuclear protein fraction? - (reply: 2)
-
Suggestions for experimental or in silico validation related to ncRNA or protein - (reply: 1)
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desired protein is going in pellet...do not want to go for renaturation - (reply: 3)
-
Concentrating protein of a particular size - (reply: 1)
-
Extra bands after protein expression/purification - (reply: 4)
-
96 well protein expression - (reply: 1)
-
Different cell lines different protein stability - (reply: 1)
-
Better protein Expression? - (reply: 2)
-
Anti-human IgG phosphatase give negative results - (reply: 13)
-
protein extraction from transwell inserts - (reply: 2)
-
Expression of protein with pI 8.2 - (reply: 1)
-
Failed BCA protein assay, white crystalline solid after speedvac - (reply: 1)
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Methionine for protein expression - (reply: 3)
-
Protein expression in yeast - Yeast Genotype - (reply: 1)
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Carrier protein concentration - (reply: 2)
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protein - (reply: 1)
-
Anti-his antibodies - Noob to protein work - (reply: 10)
-
Protein localization studies - (reply: 5)
-
Protein Expression problems - (reply: 4)
-
Cell Envelope Proteins Smeared - (reply: 1)
-
Round/flat-bottomed 96-well plates for BCA chemiluminescence protein detection - (reply: 2)
-
Problem with merged bands within western blot caused by amount of protein loaded - (reply: 7)
-
predict possible protein protein interaction - (reply: 1)
-
Cellular localisation of a protein - (reply: 1)
-
Sequential phosphorylation in vivo but not in vitro - (reply: 4)
-
problem in expressing A DNA binding protein - (reply: 6)
-
Detection of glycosylations on Western blotted proteins - (reply: 2)
-
Problems with protein estimation and western blots - (reply: 1)
-
histone proteins extraction and 2D - (reply: 1)
-
Quantity of protein to charge - (reply: 1)
-
Determining the N- and C-terminal regions in a protein - (reply: 6)
-
Properties of amino acids and protein functional activity - (reply: 1)
-
Phosphorylation - (reply: 2)
-
Protein tagging with GFP/effect of expression construct - (reply: 2)
-
RNAi problem - Gene is knocked down, but not protein - (reply: 9)
-
Insoluble Protein purification - (reply: 1)
-
Does DNA have to be supercoiled for transient protein expression? - (reply: 4)
-
my protein bands and protein marker becomes abnormally clearer sometimes - (reply: 3)
-
Missing expression of proteins of interest in Pichia pastoris - (reply: 2)
-
BLASTing protein block - (reply: 1)
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IgG control - (reply: 6)
-
Difficulty in expressing fusion protein - (reply: 1)
-
IgG control - (reply: 4)
-
Native PAGE, interaction of proteins, validity - (reply: 4)
-
what is the molecular weight of fusion protein in pET-32a - molecular weight of - (reply: 1)
-
Peptide Protein Conjugation - (reply: 2)
-
Same non specific band for different target proteins - (reply: 1)
-
Types of 96-well plates used for BCA protein quantification - (reply: 1)
-
Protein measurements in 2% Triton X samples - (reply: 2)
-
No protein in cytoplasmic extract - (reply: 1)
-
Size and composition of spacer between protein and tag? - (reply: 4)
-
Optimising recombinant protein expression level in Saccharomyces cerevisiae - (reply: 1)
-
protein extraction from pirmary keratinocytes - (reply: 2)
-
his tagged protein - (reply: 1)
-
How to purify unstable protein? - (reply: 10)
-
unstable flag tagged protein - (reply: 4)
-
Reusing scrapers + needles & syringe for total protein isolation - (reply: 1)
-
SDS-PAGE of negatively charged protein - (reply: 4)
-
GFP tagged protein expression detection - (reply: 1)
-
How to obtain high-level production of low molecular weight recombinant proteins - (reply: 1)
-
How to quantify the protein amount for serum samples ? - (reply: 2)
-
Insoluble Protein - (reply: 5)
-
making recombinant protein - (reply: 2)
-
Problem with expression of recombinant protein - (reply: 3)
-
how to make a recombinant protein with signal peptide - (reply: 1)
-
Large protein blot ~400kDa - (reply: 3)
-
Protein folding affected by neg. cahrges amino acids? - (reply: 4)
-
getting rid of imidazole in purified protein? - (reply: 2)
-
which gel concentration can separate the 7.5 kDa protein? - (reply: 6)
-
Western Blotting of Proteins less than 10 kDa - (reply: 1)
-
Protein wight in kDa - (reply: 2)
-
BN-PAGE of purified membrane associated protein - (reply: 5)
-
Expression of protein X varies in different w.b running (in one cell line, but - (reply: 3)
-
Mitochondrial and cytosolic protein isolation and purification - (reply: 1)
-
Mitochondrial and cytosolic protein isolation and purification - (reply: 1)
-
Problems eluting a GST fusion protein in GSTrap FF - (reply: 6)
-
My protein samples are running abnormally on a SDS PAGE - (reply: 3)
-
Fusing permanently a phosphate molecule to a protein. - (reply: 4)
-
Bacterial expression: incorporating radioactivity into overexpressed protein - (reply: 1)
-
Can proteins with sizes smaller than 60 kda be seen as bands in a 6% gel western - (reply: 3)
-
Protein of 160 kda is present on 6% gel but gapdh housekeeping protein is not. - (reply: 6)
-
ChIP-PCR: Amplification in IgG negative control - (reply: 1)
-
Precipitations in protein dialysis - (reply: 1)
-
mRNA increase and protein expression - (reply: 10)
-
large protein - (reply: 3)
-
applying cell culture protocol of protein extraction for rat intact tissue is co - (reply: 1)
-
A problem with IgG purification by Protein G chromatography - (reply: 4)
-
problem with loading the protein on to SDS-Gel - (reply: 1)
-
anti-GLUT4 antibody, extracellular epitope? - (reply: 1)
-
Protein contamination with DNA - (reply: 5)
-
Western blot not showing protein - (reply: 2)
-
Western Blot not showing protein - (reply: 4)
-
No activity of fungi protein in E.coli - (reply: 15)
-
longer running of proteins in SDS PAGE! - (reply: 2)
-
Rehydrating/Resuspending Drosophila Proteins - (reply: 1)
-
How exactly does an IgG antibody control in ChIP work? - (reply: 3)
-
PacI affect protein translation? - (reply: 1)
-
ELISA Kits for proteins in brain homogenates - (reply: 6)
-
Do we really need to do protein quantification - (reply: 4)
-
Maximum number of proteins - (reply: 1)
-
protein did not knock down by shRNA, but RNA did knock down - (reply: 4)
-
Western Blot for 320KD Protein - (reply: 4)
-
Can someone recommend a good proteomics company/facility with Orbitrap and can p - (reply: 1)
-
Dot Blot - Protein not adhering to membrane - (reply: 1)
-
Smears after protein precipitation - (reply: 4)
-
protein expression level and OD600 in E.coli - (reply: 1)
-
Protein contamination - How to remove all protein from my DNA? - (reply: 3)
-
FACS Sorting and Protein Purification - (reply: 5)
-
How to extract total protein from gram negative bacteria for SDS page and BCA an - (reply: 2)
-
biotinylated protein extracts and antigen-antibody stringency - (reply: 3)
-
overexpression FLAG-tagged protein shows extra bands in WB - (reply: 2)
-
Protein purification buffer - (reply: 3)
-
Protein purification in native conditions - (reply: 1)
-
Membrane proteins - (reply: 6)
-
Protein extraction efficiency - (reply: 2)
-
Triton X-100 and Dialysis - (reply: 2)
-
homology proteins - (reply: 1)
-
Improving expression of recomb proteins in baculovirus - (reply: 1)
-
Inhibition of Protein Synthesis in Eukaryotes - (reply: 1)
-
Trizol and Protein extraction - (reply: 2)
-
Protein Extraction SDS-Buffer Pellet not Dissolving and Laemlli Bufffer Green? - (reply: 3)
-
Do amino acids and his tag on vector affect protein of interest? - (reply: 4)
-
Western Blot Two Proteins - (reply: 1)
-
Western Blot Protein Length - (reply: 4)
-
Increase toxic protein expression? - (reply: 1)
-
Recombinant protein from cell lysate - (reply: 4)
-
Gooey DNA is protein samples after adding sample buffer - (reply: 1)
-
pET47b proteins not expressing in BL21 (DE3) - (reply: 5)
-
Thrombin removel His tag protein digestion - (reply: 1)
-
protein expression vector.. - (reply: 4)
-
ChIP-Seq with HA-tagged protein - (reply: 1)
-
Fc fusion protein smearing on SDS PAGE - (reply: 2)
-
protein solution cloudy after dialysis - (reply: 5)
-
Using protein content to standardize ELISA data? - (reply: 2)
-
How to improve protein solubility in E. coli? - (reply: 11)
-
RNA protein complex interaction in 4-12% NU PAGE gradient gel - RNA PROTEIN bEHAVIOR IN GRADIENT GELS (reply: 1)
-
methods for protein purification - hi.. can i know different method used for protein purification (reply: 1)
-
type of IgG in my sample?? - proteinA purified sample (reply: 1)
-
To isolate DNA, RNA and Protein from same culture flask for methylation study? - (reply: 2)
-
Help needed with stable cell line creation - problems making a cell line expressing a viral protein (reply: 3)
-
Protein structure prediction - (reply: 2)
-
Help with small protein with his-tag purification - (reply: 7)
-
protein precipitate at pI?? - (reply: 1)
-
Protein agregation in WB samples - (reply: 3)
-
Lentiviral transduction, no target protein expression and lost other reporter - (reply: 1)
-
Protein dissolved - After acetone precipitation (reply: 2)
-
Max amount of protein - (reply: 4)
-
TCA/Acetone protein precipitation - (reply: 9)
-
how to add 3XHA to the N-terminal of a protein - (reply: 5)
-
Separating protein isoforms and quantifying activity - (reply: 3)
-
Protein refolding - (reply: 2)
-
Freezing proteins at -80 - (reply: 1)
-
Overrepresentation of genomic region in ChIP IgG control?! - (reply: 2)
-
Discrimination against denatured proteins?! - Can ELISA discriminate agains denatured proteins? (reply: 2)
-
Chromatin Protein IP - (reply: 2)
-
How can I detect mannosidase activity of a protein - (reply: 1)
-
Protein Aggregation with 6xHis tagged CreGFP - (reply: 1)
-
Problem with protein resolving in electrophoresis - (reply: 7)
-
Protein stability at room temperature - (reply: 3)
-
Help, problems with expressing human proteins in E.coli - IPTG induced cells turn pink (reply: 1)
-
protein pass through column - (reply: 1)
-
Help with protein expression - how to optimize expression of large GST fusion protein (reply: 4)
-
protein precipitation - isolation of proteins (reply: 1)
-
effect of lysozyme on gst-fusion protein - (reply: 1)
-
Protein purification from HEK 293 cells - (reply: 1)
-
Odd result help - Luciferase / protein / mRNA levels (reply: 1)
-
Upscaling protein expression in E.coli - (reply: 2)
-
Dot-blot with immobilized ABs on membrane for protein detection - (reply: 8)
-
confirm proteins interacting with histones using acid extraction - (reply: 4)
-
Housekeeping protein issues - Is my experiment affecting my housekeeping protein levels? (reply: 1)
-
nuclease contamination in protein prep - (reply: 4)
-
Bio Rad protein assay for liver homogenate - (reply: 1)
-
protein purification - (reply: 1)
-
Bradford Vs beta-actin for WB protein quantification - (reply: 3)
-
Dual luciferase assay - proper controls? - LUC below pGL3 despite protein activity (reply: 4)
-
SDS Page heat up and "fry" the gel and protein (Western Blot) - (reply: 2)
-
acrylamide gel for low molecular weight protein - (reply: 2)
-
ELISA kits protocols, antibodies, control proteins, and more - (reply: 1)
-
HELP! TISSUE STORAGE AND PROTEIN DEGRADATION! - (reply: 1)
-
Pierce BCA Assay problem - Protein values bizarre (reply: 3)
-
plant protein purification opinion - (reply: 3)
-
WB - Transferring REALLY big proteins - (reply: 2)
-
Unable to Purify protein! - Protein purification (reply: 2)
-
Cloning and expressing a mystery 71KDa protein - (reply: 9)
-
HIS-tagged protein purification - (reply: 12)
-
how to elute "only" my recombinant protein - final elutes comes with non-specific proteins... (reply: 4)
-
Is it possible to islate strong negative charged protein - (reply: 1)
-
Protein expression vectors Please help - (reply: 2)
-
what is protein flexibility - (reply: 1)
-
His-tagged protein purification problem - (reply: 2)
-
protein level expression - (reply: 1)
-
How to get rid of DNA/RNA in protein expression preps - Is there an alternative to DNAse? (reply: 1)
-
Looking for protocol to do biotinlynation on each single protein in cell lysate - (reply: 1)
-
proteins, new ones? - How to know (reply: 8)
-
KO mice show band for knocked-out protein - (reply: 1)
-
Trouble in protein quantification - (reply: 2)
-
SDS-PAGE gel wont separate small proteins - (reply: 5)
-
LIC cloning - pETDUet LIC insertion for dual protein expression (reply: 2)
-
Unexpected expression of my protein in HepG2 - (reply: 3)
-
Protein Aggregation, Sample Boiling, help! - Western Blotting (reply: 4)
-
EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
-
Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
-
GST-fusion protein degrades in-vivo - (reply: 3)
-
Purification of recombinant proteins from insect cells - (reply: 2)
-
Non reducing protein ladder? - (reply: 5)
-
similar size of proteins but different movement on SDS-PAGE - (reply: 1)
-
Protein Expression in yeast - protein not expressed (reply: 1)
-
SDS-PAGE: Effect of Acrylamide concentration - Is it different for high and low MW proteins (reply: 2)
-
no detection of proteins at 25KDa - (reply: 4)
-
help with protein elution - (reply: 1)
-
Protein quantification after 8M urea lysis - (reply: 3)
-
phosphorylted protein smaller not bigger? - (reply: 1)
-
protein phosphorylation status in sample buffer - (reply: 2)
-
Protein detection - western blot (reply: 3)
-
Boiling, protein aggregation......reversal?? (western) - (reply: 3)
-
expression of protein - (reply: 1)
-
Tag proteins for CO-IP and confocal - (reply: 1)
-
insoluble protein expression help - (reply: 3)
-
Active protein extract from corn - (reply: 2)
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Model Protein - Need Advice! - (reply: 3)
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You have truncated protein domain that won't express? - If you wanted to express a protein but failed because domain not intac (reply: 1)
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CHOP protein western blot...help reqd! - transfer conditions for CHOP protein (reply: 3)
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His-tag protein vs. BSA - (reply: 3)
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Thrombin cleaved GST-tagged protein remained bound to beads! - GST protein purification Thrombin (reply: 7)
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YFP protein fusion accumulates - (reply: 10)
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Protein Concentation from M to mg - (reply: 2)
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Storage condition of Recombinant protein (native) - suggestions? (reply: 5)
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Problems in recombinant protein expression - Problems in recombinant protein expression (reply: 5)
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protein interactions - (reply: 2)
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MTT assay, protein assay analysis - Need assistance with analysis of these two assays (reply: 6)
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Lactobacillus crude protein extraction? - (reply: 8)
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Problem of recombinant bacterial membrane anchored protein - Recombinant protein (reply: 2)
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Western Blot of Large Protein Help - (reply: 1)
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Fusion tagged protein and Western Blot problem - (reply: 3)
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Compatible detergents for proteomics - (reply: 4)
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Troubleshooting Protein Purification using FPLC - (reply: 1)
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how to make protein soluble? - protein solubility (reply: 4)
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Quantification of proteins in Western Bands - (reply: 2)
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protein yield is very low after purification - protein purification (reply: 9)
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his- & biotin- tag protein purfication - (reply: 2)
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How to remove protein to show an activity is protein based - (reply: 7)
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FZR1 PROTEIN EXPRESSION - (reply: 1)
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help with his tagged protein purification - precipitation problem (reply: 3)
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Overexpression plasmid is not expressing protein - (reply: 13)
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Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
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why phosphorylated protein relative to total protein? - (reply: 2)
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SDS-PAGE with 500 ug protein - (reply: 3)
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Heterologous protein extraction in yeast for enzymatic activity test - (reply: 5)
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His tag protein purification - (reply: 7)
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Dry protein gels without cracking - (reply: 5)
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Backgrounds persistence and higher than target protein in western blot - Expressed in BL21(DE3) (reply: 14)
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insoluble fusion protein - (reply: 1)
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Protein precipitating need help choosing suitable buffering system - (reply: 3)
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Total Cell protein - (reply: 1)
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ammonium precipitation of Proteins - (reply: 2)
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Expressing human protein in E.Coli - (reply: 12)
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Assay in Blood - sticky protein (reply: 13)
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dye protein ladder - (reply: 5)
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protocol to transfer protein on transfer membrane fluoride bind - please tell me how to transfer proteins from sds page (reply: 4)
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Gel gets squiggly bands for low molecular weight proteins - (This is driving me insane) (reply: 7)
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Naming system of IgG and Identification of IgG subclass - (reply: 5)
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How do you identify protein detected on western blot from a lysate???? - (reply: 2)
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how to express protein in soluble form - (reply: 1)
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Help with GST protein purification - (reply: 4)
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Antiobiotics for protein expression - (reply: 3)
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Number of proteins per cell? - One of THESE questions... ;) (reply: 1)
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IP no target protein in elution - (reply: 1)
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protein function prediction - (reply: 5)
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stabile transfection, all have high mRNA level, only ~ 50 % have protein - (reply: 1)
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Protein stability - (reply: 4)
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PROTEIN QUANTIFICATION - cell lysis buffer (reply: 2)
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Protein isolation in zebrafish - (reply: 1)
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how to extract proteins from HEK293 - I need your experience (reply: 3)
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Identification of purity of the protein - (reply: 2)
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ChIP - protein contamination of ChIP'ed material - (reply: 4)
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Biotinylation of protein in tris buffer - (reply: 2)
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Maltose Binding Protein Sequence - A sequence for the Maltose Binding Protein. (reply: 1)
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HELP!?!? with MEMBRANE PROTEIN extraction - Extraction of GPCRs from HEK293T for IB and IP (reply: 1)
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Protein kinase protocols - (reply: 2)
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Identification of Membrane Receptor protein, how ?? - I wanna use MS approach ! (reply: 4)
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Purification of His tagged protein - (reply: 2)
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BCA diluent and standard curve - (reply: 1)
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protein induction problem - (reply: 4)
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Loss of protein expression following double transformation - Protein expression post transformation (reply: 10)
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Protein purification. How to make function of recombinant protein? - (reply: 2)
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Is My Protein Pure or Not? - Protein purification, SDS-Page (reply: 19)
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Primary Mouse antibody on mouse tissue - How to use mouse antibody to do western on mouse protein (reply: 2)
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ChIP doesn't work. Please help! - Too much "normal rabbit IgG" bockround (reply: 8)
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Protein expression declines after overnight incubation - (reply: 4)
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Help! Cell homogenization and protein extraction for Western Blotting - (reply: 2)
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elimination of Protein interference from DNA - for RT-PCR (reply: 2)
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Fusion protein linker - (reply: 2)
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common reasons proteins show up at different sizes on blot - what are they? (reply: 1)
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Homogenization of articular cartilage prior to protein extraction - (reply: 1)
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Protein Phosphorylation - (reply: 2)
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Western for very small protein - (reply: 3)
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Protein streaking on Blue Native-PAGE - (reply: 9)
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basic questions about protein assays and cuvettes - (reply: 4)
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His-Tag protein purification:protein eluted with NaOH??? - (reply: 3)
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antibody production-how to differentiate epitope as foreign - (reply: 4)
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How to attain pure chains of one protein complex - (reply: 1)
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MINI Dialysis Units - below 2K MWCO? (reply: 1)
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Help for co-transfection of proteins and plasmid - (reply: 1)
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Problems with precipitation of proteins using TCA/aceton - (reply: 3)
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Fusion Protein - (reply: 3)
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Solubility of proteins - (reply: 1)
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Loss of protein upon concentration - (reply: 4)
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protein overexpression in HeLa cells - (reply: 1)
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His-tag protein purification - aggregation of protein (reply: 5)
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After inducing with IPTG for protein expression, my cell pellet after centrifuga - (reply: 1)
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CoIP using Ni NTA, my non-his-tagged protein bind to beads! - (reply: 1)
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High mRNA but no protein expression - (reply: 10)
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Protein Purification of an His-Tag Protein using a Ni Column - (reply: 1)
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Baculovirus Expert Needed (Occlusion bodies) - Can proteins become stuck in occlusion bodies in baculovirus? (reply: 6)
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Decrease in concentration after protein purification - (reply: 3)
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Circular Dichroism and disordered proteins - (reply: 1)
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2D-PAGE of G protein coupled receptor - Need advice on sample preparation - GPCR overexpressed in HEK293 cells (reply: 1)
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Bacterial Induction: Same concentration of protein samples? - (reply: 1)
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protein phosphorylation - methods for protein phosphorylation (reply: 9)
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Unable to get proper PCR amplification - PCR does not correlate protein expression (reply: 3)
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labeling a protein - (reply: 3)
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Co-IP of secreted proteins - (reply: 1)
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protein protein interaction discovery - (reply: 1)
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what is the best way to detect protein expression levels in plants - Also which company provides the best services on antibody production? (reply: 1)
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Problem in stable expression of target protein using lentiviral vector - (reply: 3)
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How to be good at prep? - Calling all protein experts (reply: 2)
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Protein Migration Problem [S.O.S.] - Moves in SDS-PAGE but not in Native (reply: 8)
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His-tagged protein purification - (reply: 3)
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143 kDa protein - (reply: 3)
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Do you get insoluble proteins also in whole cell lysate? - (reply: 3)
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protein purification - (reply: 3)
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Purification od insoluble protein (TIMP3) - (reply: 2)
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eluting gst fusion proteins - (reply: 1)
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How to pull down chromatin and identify protein associated with it? - (reply: 1)
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contamination in protein expression - I got a couple of questions (reply: 3)
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Protein extraction from perfusion fixed brain tissue - (reply: 5)
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Always expressing truncated protein - (reply: 3)
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Antibody production. - Discrimination between almost identical proteins. (reply: 1)
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secreted protein quantification - (reply: 2)
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protein sample degradation - (reply: 7)
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Major Protein Loss! Why? - (reply: 2)
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Protein in the nucleus vs in the cytoplasm - (reply: 1)
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Over Experssion of A Protein - (reply: 3)
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Homogenization for Plasma Membrane Protein Extraction - (reply: 1)
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problem in protein purification - (reply: 5)
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Protein Gel Resolution - Unable to see proteins with a molecular weight less then 14 kDa (reply: 4)
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Effect of DTT on the affinity of Protein A/G and immune complex - Effect of DTT on the affinity of Protein A/G and immune complex (reply: 2)
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protein purification - (reply: 5)
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Cloning the coding region of a protein - (reply: 4)
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Problem with protein purification By FPLC - (reply: 4)
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Molar ratios of IgM and IgG antibodies in in vitro assays - (reply: 3)
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cytoplasmic and nuclear extraction for BCA - (reply: 6)
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Problem in expressing AKAP scaffold proteins in mammalian cells - Expression of AKAP scaffold proteins (reply: 1)
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EGFP protein location problem fixing with PFA - (reply: 3)
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Isolating bacterial RNA and protein from the host tissue sample - (reply: 3)
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protein prep - about prptein preperation in diagnosis (reply: 1)
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Protein dephosphorylation with wich Phosphatase?... and what lysis buffer? - (reply: 1)
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loss of protein during concentration - (reply: 6)
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protein degradation - (reply: 3)
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Cell lysates - Protein precipitation? - (reply: 3)
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Protein precipitation with TCA? - Protocols, suggestions, ideas, experiences (reply: 2)
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His-tag proteins not eluted from Ni-Beads - (reply: 2)
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Is CNBr activated Sepharose good for binding IgG? - (reply: 3)
-
Western Blots on Membrane Proteins - (reply: 9)
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Samples for Western to detect phosphorylation of EGFR - (reply: 2)
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quantification of a protein in tissue - (reply: 3)
-
protein sequencing company - (reply: 2)
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Protein extraction from mouse prostate - (reply: 3)
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Help - proteins faster than the dye front - (reply: 8)
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fluorescent proteins expressed in nucleus - (reply: 6)
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IGg or F(ab´)2 Fragment of IgG - HRP-conjugated secondary antibody (reply: 1)
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A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
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Using FASTA Seq to Compare protein Seq. - (reply: 1)
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Protein SDS-PAGE thick bands / smear - (reply: 4)
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Denaturing protein before blotting - (reply: 1)
-
not completed transfer proteins on NT membrane - western blot (reply: 2)
-
trouble with protein expression of high AT rich gene - (reply: 1)
-
No protein lane have bands on Coomassie and western blot?! - (reply: 4)
-
Protein cleavage and neurodegeneration. - (reply: 1)
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Extraction protein from paraffin-embedded tissues - (reply: 1)
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column chromatography - elution buffer for proteins (reply: 2)
-
Lost protein after gel filtration - Gel Filtration (reply: 6)
-
protein size - (reply: 1)
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is my lost protein is bcoz of cell death or my treatment worked??? - (reply: 2)
-
New smeared bands for protein that previously ran fine - Smeared protein bands now but not the previous 50 times I've run same (reply: 1)
-
Weird effect of siRNA on protein in cells - (reply: 3)
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estimate size of protein - (reply: 2)
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Protein elution from GST-fusion protein complex - (reply: 3)
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Problems with Soluble and Insoluble Protein Fraction - (reply: 4)
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total cell protein vs membrane protein - (reply: 4)
-
amount of plasmid for transformation and fusion protein expression - (reply: 1)
-
gst fusion protein - (reply: 3)
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Biological process from protein list - (reply: 3)
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how to get pure protein - (reply: 1)
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Liposome + IgG sample in SDS-PAGE - (reply: 1)
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Different centrifugation duration, different protein?? - (reply: 1)
-
Protein tags - (reply: 6)
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What portion of protein can I get under this protocol? - (reply: 3)
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SDS-PAGE for proteins which have pI>7 - (reply: 1)
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looking for kinase inhibitor to study phosphatase - kinase inhibitor (Inhibit phosphorylation on transmembrane receptor) (reply: 4)
-
to get protein in cytosol from inclusion bodies - (reply: 4)
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How can I get protein in soluble fration - (reply: 2)
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Looking for precipitation method to reduce the volume of my protein complex elu - TCA precipitation seems to be hard to be resolved again (reply: 6)
-
How long does it takes to make a crystallography of a protein? - (reply: 1)
-
how to detect a knowen protein's receptor? - (reply: 1)
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Is IgG monoclonal antibody better then IgM? why? - why everyone prefer IgG? (reply: 3)
-
protein purification - removing salts, detergents, etc from a protein sample (reply: 6)
-
How much protein from a confluent 6 well plate? - (reply: 5)
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Protein expression - (reply: 8)
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Protein quantification - (reply: 2)
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immunochemistry - using cofocal to identify two protein localization (reply: 1)
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Protein samples-best way to store - Using Denator Stabilizor T1 or other instruments/methods (reply: 1)
-
Wet Transfer is not transferring 300kDa protein, why? - problems with wet transfer (reply: 11)
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Bands are lower than actual protein - (reply: 1)
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Protein lysate storage - (reply: 2)
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to bring protein in soluble fraction - my protein is cysteine rich and i am getting it in insoluble fraction. (reply: 1)
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Tricine SDS-PAGE for plant mitochondria - Membrane proteomics (reply: 1)
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Protein Purification by precipitation - (reply: 2)
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refolding proteins using dialysis - (reply: 1)
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protein refolding - (reply: 1)
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concentrating protein by method other than tff - (reply: 1)
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Protein TAG for affinity purification - (reply: 1)
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BCA Assay and SDS-PAGE Gels Do Not Agree - (reply: 2)
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Quantitating IP'd proteins - (reply: 1)
-
help with protein localization study using GFP - (reply: 6)
-
Protein shows KD but RNA level is higher - (reply: 4)
-
No protein spots in 2D gel - Silver staining! - (reply: 17)
-
Help!! BCA kit v.s Bio-Rad kit for protein assay - (reply: 1)
-
Wound healing (scratch) assay - extracellular matrix protein substrate (reply: 1)
-
protein crystallography services question - (reply: 1)
-
c1q ? Protein A? Or Protein G for immune complex detection? - immune complex detection (reply: 2)
-
Problem in expression of protein cloned in pcDNA3 - (reply: 1)
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Recombinant protein aggregation after purification - (reply: 2)
-
average total protein content per 293t cell - (reply: 2)
-
best and simple method to purify protein - (reply: 38)
-
Protein A Sandwich ELISA - Protein A orients Capture Ab-Antigen-primary Ab-detector Ab (reply: 1)
-
Localization of kanamycin resistance protein - (reply: 1)
-
Are proteases a problem in protein quantification assays? - (reply: 3)
-
why my protein precipitates? - (reply: 2)
-
serum housekeeping proteins for total protein normalization - (reply: 1)
-
serum housekeeping proteins for total protein normalization - (reply: 1)
-
protein concentration per well - (reply: 6)
-
inclusion body purification kit - GST tagged insoluble proteins (reply: 2)
-
Detection of phosphorylation site - (reply: 23)
-
help my proteins are disappearing from my PVDF membranes - SDS Page help (reply: 1)
-
Sites for protein sequence from DNA sequence - (reply: 2)
-
will high-speed centrifuge rupture protein G? - (reply: 1)
-
His-tag protein expression - (reply: 1)
-
Does Change pH could affect protein interaction - GST pul down (reply: 2)
-
Incorporation of stable isotope aminoacid in protein synthesis in vitro assay - (reply: 1)
-
protein extraction - ripa buffer vs urea buffer (reply: 1)
-
sucrose gradient for protein fractionation? - sucrose gradient (reply: 1)
-
problem about fused protein - problem about fused protein (reply: 2)
-
Diffused protein in IF stainning - (reply: 2)
-
IP my protein from culture supernatant - (reply: 2)
-
How to concentrate the protein - (reply: 4)
-
Western blot: problem with protein migration - trouble shooting......... please help! (reply: 10)
-
High pI proteins - High pI proteins (reply: 4)
-
protein overexpression - factors affecting overexpression (reply: 3)
-
NUCLEAR PROTEIN PREPARATION - (reply: 1)
-
High Gene low protein levels - (reply: 1)
-
Lost protein from blot? - (reply: 1)
-
293 vs 293TN cells - Which would be beter control in testing against expressed proteins (reply: 1)
-
Protein extraction buffer compatible with 2D PAGE - (reply: 4)
-
Protein Degradation after overexpression - (reply: 1)
-
Protein Extraction from Tissue/ Lysate storage - Several questions regarding lysis buffer usage and storage options (reply: 4)
-
e coli strain selection for protein expression - (reply: 2)
-
phosphorylation of primers using PNK - (reply: 1)
-
IgG SDS-PAGE - (reply: 1)
-
SDS-PAGE: Protein bands broaden as it migrates down the gel like a pyramid! - (reply: 2)
-
wet or semi-dry transfer for low MW proteins - (reply: 4)
-
Problems in upscaling protein expression - (reply: 2)
-
Where is the future of the proteomics? - (reply: 5)
-
problems with His tagged protein western blots - (reply: 3)
-
BSA Standard Curve Dilution (BCA Kit) - how to dilute the correct way? (reply: 6)
-
Protein aggregates SDS-PAGE - (reply: 5)
-
check of amount of proteins on filter after rouge ponceau| - (reply: 1)
-
dna loading dye for proteins - (reply: 2)
-
too many isoforms expressing proteins - (reply: 2)
-
protein optimization - (reply: 3)
-
Protein stabilization buffer needed - (reply: 1)
-
Freezing eluted His tag protein - (reply: 5)
-
molecular cloning troubleshooting - bacterial sequences instead of my protein ! (reply: 3)
-
Protein Markers - (reply: 1)
-
What if my expressed protein is toxic for the cells... - (reply: 1)
-
His-tag protein lost it's tag - (reply: 2)
-
AnionExchange problem: proteins do not bind - (reply: 12)
-
Dialysis into Lipid - (reply: 2)
-
How can I isolate ECM protein from a 6 well dish? - (reply: 4)
-
How much protein should be loaded to the column during the purificiation? - Is less more? ;) (reply: 1)
-
can someone list some proteasome inhibitors? - co-IP for ubiquitiantion protein (reply: 4)
-
GST fusion protein expression - Expression problems using pET41a vector (reply: 1)
-
Protein standard curves - (reply: 1)
-
Primer for Unkown Protein PCR - How to design primer for unknown protein (reply: 2)
-
Western Blot for low expressed protein - (reply: 4)
-
radiolabeled protein uptake - (reply: 2)
-
small proteins have run ahead of tracking dye - (reply: 1)
-
Generation of ChIP grade antibody - Purification of recombinant protein (reply: 3)
-
Precipitation of protein - (reply: 3)
-
in vitro phosphorylation - (reply: 5)
-
Meaning of Recombinant Protein - (reply: 3)
-
Measuring DNA, RNA, protein and cell wall synthesis - (reply: 1)
-
Equal Protein Quantity - Loading onto Well (reply: 6)
-
Desperate! Loss of basic proteins on 2D gels - (reply: 5)
-
additional proteins in eluted protein-protein purification of GST tag fusion pro - (reply: 1)
-
protein absorbance at 230nm??? - (reply: 1)
-
How to look at protein stability? - (reply: 3)
-
PEG Precipitation of IgM Antibodies - (reply: 2)
-
Fermentas prestained protein marker issue! - (reply: 7)
-
reprobing a membrane to detect a protein with the same molecular weight - (reply: 4)
-
Expression of fusion proteins - (reply: 1)
-
Help with protein extraction buffer problem!? - (reply: 1)
-
Purifying Protein Samples to Remove or Reduce SDS? - Need to remove or reduce SDS in protein extract so ELISA will work! (reply: 3)
-
Purification of Protein Extract for ELISA - (reply: 1)
-
Protein A-agarose or A-sepharose for immunoprecipitation? - (reply: 3)
-
High protease activity in protein extraction - A problem in western blot (reply: 2)
-
Number of proteins expressed in cell - (reply: 2)
-
Deep sequencing to find gene targets of protein? - (reply: 1)
-
Protein at low PH - Strange behavior of His-tagged protein at low PH (reply: 1)
-
How to store wet dialysis membranes once opened? - (reply: 4)
-
bands of protein seems like it did not move - (reply: 2)
-
Constitutive association between two proteins - (reply: 2)
-
Elute immunoprecipitated complex with protein G? - (reply: 1)
-
protein indentification - protein to be identified on sds page based on MW (reply: 2)
-
Help...purify and concentrate protein for IEF - (reply: 1)
-
Antibody for Fusion Protein - Antibody/ies against fusion protein (reply: 2)
-
Could a 30aa peptides(3×flag+6×his） be expressed by BL21 and be successfu - What is the minimum of the length of expressed protein? (reply: 1)
-
NP40 or Triton as membrane protein detergent - (reply: 2)
-
What should be the goal amount of protein to get from purification? - Trying to figure out how much culture I need to make (reply: 3)
-
Cloning -> expression -> i see multiple bands :( - overexpression of my 3x flag tagged protein shows 4 bands.. (reply: 5)
-
Introduction to x-ray crystallography of proteins - (reply: 1)
-
Problem in transfer high MW protein - (reply: 5)
-
precipitation of secreted protein from growth media - (reply: 1)
-
No IgG Signals in immunostaining - urgent (reply: 2)
-
how to design primer for my n-terminal sequence - hot to design primer for my protein (reply: 2)
-
dimerization or aggregation of my protein! - (reply: 3)
-
GST-tagged protein binds to column but is not eluted - (reply: 1)
-
IGg bands on SDS PAGE - (reply: 2)
-
Analyzing outer mitochondrial membrane proteins via WB - (reply: 6)
-
OKADAIC ACID for protein prep - (reply: 1)
-
urea-denatured protein precipitates after dialysis - (reply: 1)
-
Expressing 4 proteins in an operon - (reply: 6)
-
Protein Transfer Problems from SDS-PAGE Gels - Involves Coomassie Staining (reply: 15)
-
Protein concentration for crystallization - (reply: 1)
-
How can I decrease IgG background? - My IgG background is too high!!! (reply: 12)
-
TCA and protein percipitation? - (reply: 2)
-
Co-immunoprecipitation: how to increase amount of interacting protein? - (reply: 4)
-
Why does the molecular weight of my protein shift *up* after running it over a H - (reply: 4)
-
Protein precipitates upon dialysis - (reply: 5)
-
protein separation by filtration - (reply: 3)
-
What is the epitope of acetylated tubulin antibody? - (reply: 6)
-
Amount of chromatin for IgG control - URGENT! (reply: 1)
-
membrane protein aggregation - (reply: 1)
-
Loading Equal protein concentrations - Does it make a difference in simple overexpression checking? (reply: 4)
-
Problem with transferring proteins from nonreducing SDS-PAGE gel - Help please: proteins are not transferred :-( (reply: 7)
-
Protein Expression in stationary phase - (reply: 11)
-
Protein Smearing - (reply: 2)
-
after purification/storage/buffers... - How not to lose protein after purification (reply: 3)
-
His Tag Protein Purification - (reply: 6)
-
Non-deacetylable Protein? - Is this possible? (reply: 3)
-
Trouble about overexpression by lentiviral transduction - Why no protein of interest expressed!? (reply: 1)
-
Amylose bound protein elution - (reply: 1)
-
Protein elution from amylose resin - (reply: 1)
-
Problem: protein precipitates from cell lysate after defrosting - (reply: 4)
-
Does anyone use potassium acetate to precipitate protein? - or other acetate (reply: 1)
-
Overexpressed protein not detected - Oh, the frustration... (reply: 1)
-
Cellular proteins in medium - (reply: 1)
-
Western Blot Antibodies for inhibiting protein activity?? - (reply: 2)
-
Detecting phosphorylated Erk1/2 - (reply: 6)
-
save me with the western blot to detect phosphorylated Jak2 and STAT4 - (reply: 3)
-
yield vs concentration of protein - (reply: 3)
-
cys rich protein expression - (reply: 1)
-
insect protein expression - (reply: 1)
-
Coupling of protein to NHS activated HP column (GE healthcare)? - (reply: 5)
-
protein extraction from tissue, help - (reply: 1)
-
Protein extraction from tissue - (reply: 1)
-
Transport & storage of protein extracts - (reply: 1)
-
Protein bands on the bottom of the gel - (reply: 5)
-
problem of membrane protein FPLC - (reply: 1)
-
my western blots of membrane protein do not work - (reply: 9)
-
IgM quantification - Monoclonal Antibodies (reply: 1)
-
Protein Expression in SF9/hi5 cells - troubleshooting protein expression (reply: 1)
-
Protein isolation from skin - (reply: 2)
-
How to concentrate my protein? - (reply: 1)
-
small protein, transfer pH - some guidelines for conditions would be great! (reply: 3)
-
Organism specific protein?? - data from maldi-ms (reply: 6)
-
buffer for membrane protein - (reply: 1)
-
Protein cloned in pRK-5 Myc and pcDNA3.1 Myc-His Tag not showing up - I need help for a transient expression system (reply: 3)
-
expression of His-tagged protein in insect cells - (reply: 2)
-
Whole cell lysate including nucelar proteins? - (reply: 3)
-
Protogel Quick-Cast - Has anybody used this to make their protein gels? (reply: 1)
-
Protein concentration and - (reply: 3)
-
membrane protein purification - (reply: 6)
-
VEGF-HEK - Protocol for collecting secreted protein (reply: 1)
-
Protein expression failing - (reply: 1)
-
How does high salt extraction of nuclear proteins work - (reply: 9)
-
Nuclear Protein Extraction - Nuclear Protein Extraction (reply: 1)
-
Dimerization of protein - (reply: 1)
-
Phosphorylated primers - (reply: 5)
-
Isolation of protein crosslinked to plasmid - (reply: 3)
-
Big differences between BCA and Bradford? - Two assays give very different results (reply: 8)
-
Contamination in GST fusion protein purification - (reply: 2)
-
Problem with protein samples - No bands detected at all on gel (reply: 2)
-
Protein localization - Protein localization (reply: 1)
-
moles —SS— groups in a protein calculation - (reply: 3)
-
Purification of scretory protein from bacteria - (reply: 2)
-
express protein with many rare codons - (reply: 1)
-
About GFP- tagged proteins in western blot - (reply: 1)
-
GST-tagged proteins - (reply: 1)
-
WESTERN BLOT OF LARGE AND MINORITY PROTEIN - (reply: 1)
-
lentiviral expression - transefection, low titier, protein expression (reply: 1)
-
No protein bands in SDS-PAGE after sample clean-up steps! HELP! - (reply: 8)
-
Protein Sample Preparation - Western Bloting (reply: 1)
-
defatting of soybean powder for protein isolation - defatting of soybean powder for protein isolation (reply: 3)
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Protein precipitates during BrCN-sepharose coupling - (reply: 5)
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Dual cross-linking thoughts - Agents to help analyse proteins indirectly bound to DNA (reply: 16)
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Blasting Primers for RT-PCR - what's hypothetical proteins? My primers always match to these!! (reply: 2)
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Protein Standard stability (BCA assay) - (reply: 3)
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IHC staining of secreted proteins - (reply: 3)
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Dimeric form of a protein? - I want my monomer! (reply: 2)
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Ni-NTA his tag protein purification - (reply: 1)
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Protein Soluble Test - Inclusion Bodies + UREA test? (reply: 3)
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protein concentration factor - (reply: 3)
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I want to buy p53 protein - which company? - (reply: 1)
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Protein identification in 2D gel - (reply: 1)
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Double Labelling of Same Protein - (reply: 4)
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problem with lentiviral expression of protein - (reply: 2)
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For those having problems with running & transfer of high MW proteins - (reply: 1)
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Protein Size - (reply: 3)
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why the bands migrate lower in the gel EMSA? - as I increase protein concentration (reply: 1)
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cloning of non-coding region for protein expression - protein expression (reply: 1)
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Protein Purification from Qiagen RNeasy Kit - Has anybody done this? (reply: 3)
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Transfer basic protein to PVDF - (reply: 1)
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Protein expression - (reply: 4)
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Protein G vs Protein A for Rabbit Antibodies - (reply: 8)
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single amino acid mutation effects on protein function - (reply: 1)
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competition of thrombin and my protein on benzamidine beads - (reply: 5)
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how to extract protein from agarose? - (reply: 1)
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Protein expression with T7 promoter - (reply: 1)
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disappearing high molecular protein and marker,thanks - (reply: 1)
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I can't resolve 2 proteins (LC3-I and II) in Tricine gels - (reply: 5)
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Is an enzyme protein of 1100 aa too big to be expressed in E coli - (reply: 4)
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co-IP of chromatin binding protein - (reply: 2)
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protein in phosphate buffer precipitate out with acetonitrile - (reply: 1)
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Protein cellular location tool - (reply: 1)
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Western blotting for proteins in vesicles? - (reply: 2)
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Protein expression using invitrogen pSecTag2 - (reply: 2)
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Protein drag in IEF-WB - (reply: 3)
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His protein expression problem - (reply: 1)
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need help cloning cdna of a known protein - (reply: 1)
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protein modelling - (reply: 1)
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Help with Western Blotting of Keratin Proteins - (reply: 2)
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Modification of Bradford Assay with SDS - THe attempt to increase sensitivity for a protein with SDS (reply: 1)
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Mock IgG versus No Antibody - Negative Control - (reply: 7)
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His-tagged protein runs much faster than expected - 6His-Tagged CcpA protein runs around 26 kD whereas I expect to run 37 (reply: 7)
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protein going to inclusion bodies - protein going to inclusion bodies (reply: 1)
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removal of SDS from protein - (reply: 4)
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prepare sample protein for 2d from E.coli - (reply: 2)
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Protein won't transfer for Western Blot - (reply: 5)
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How topurify and measure stability of mAb IgM - (reply: 11)
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PCR a plasmid protein - (reply: 2)
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To find a protein's function which MW is 14kD. - (reply: 6)
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Over Expression of GFP tagged L-Asp/ Moesin Proteins - (reply: 5)
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protein oxidation - (reply: 1)
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Protein size and gel percentage - (reply: 7)
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how to explore protein interactions - (reply: 3)
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Adventitious chemical binding - Unwanted chemicals in protein solution (reply: 2)
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Protein precipitation by TCA acid + acetone - (reply: 4)
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Non Reducing SDS protein ladder?? - (reply: 4)
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biotinylation of proteins - (reply: 1)
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Protein Free Blocking Buffers - (reply: 1)
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More than one protein induced - (reply: 4)
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Protein aggregates - Monomeric fraction varies with wavelength (reply: 4)
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Protein aggregates - Monomeric fraction varies with wavelength (reply: 1)
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protein concentration - can be measured at 280nm (reply: 3)
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could FLAG-tagged protein be used in the Luc assay? - (reply: 1)
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Exctinction Co-efficient - glycosylated proteins may be tricky (reply: 3)
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Protein quantification from paraformaldehyde-fixed cells - (reply: 2)
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Out of expected protein size in SDS-PAGE - (reply: 10)
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Does biotinylated proteins will migrate diferently in an SDS than the actual pro - (reply: 7)
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Mock (IgG) Ct Values - (reply: 3)
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Storage of overexpressed protein - (reply: 4)
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HELP_Bradford and protein problems - Different protein concentrations (reply: 1)
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precipitation of proteins from stained cells - Co-IP failure (reply: 6)
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Protein present in Western, not ELISA - (reply: 10)
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problem in the GST fusion protein purification - (reply: 4)
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how to deal with protein precipitates during dialysis - (reply: 1)
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reconstitution of membrane proteins into liposomes - (reply: 1)
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Amount of inoculum? - for protein expression in E.coli (reply: 3)
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ideas for Fluorescent tagging of 7Kda bacterial protein. - (reply: 1)
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the sensitive amount of protein for western blotting - (reply: 3)
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Concentrating Protein - 8M Urea, Protein solution, concentrating (reply: 8)
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Mouse Fibroblasts - need to extract nuclar protein (reply: 4)
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Qubit Protein Quantitation Issues - Qubit protein quantitation (reply: 2)
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How to determine final amount of 20 ng protein per WB well - (reply: 8)
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Protein extraction from plant leaves, bioactivity - (reply: 1)
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High Molecular weight protein separation - (reply: 2)
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Secreted protein for loading control - (reply: 2)
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Protein Disappeareance - (reply: 1)
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Role of imidazole in lysis buffer - for obtaining recombinant his-tag protein (reply: 4)
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Immunodepletion of high abundance protein - (reply: 1)
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How to denaturate protein for ELISA - (reply: 1)
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2D gel - amount of protein (reply: 1)
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protein size change after western blot - (reply: 1)
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Coomassie blue before protein transfer - (reply: 9)
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protein expression (temperature issue) - trying to express soluble protein at lower temperature (reply: 17)
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epitope tagging of pcr products - (reply: 1)
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densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)
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His tag Protein Elution - Elution Buffer (reply: 2)
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net charge on protein - (reply: 1)
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Can GST-tag be separated from GST-fusion protein - (reply: 1)
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Protein analog - (reply: 1)
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Increase in protein size - Increase in protein size (reply: 5)
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How to analyze the ingredients of protein bands in native-PAGE gel? - help (reply: 3)
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Membrane protein isolation - (reply: 1)
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Expression of human protein in mice in-vivo - (reply: 3)
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Help with T7RNAP purification - Cell-free protein expression (reply: 3)
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step-wise dialysis? - (reply: 11)
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membrane protein extraction buffer - (reply: 1)
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Large protein transfer problem - (reply: 8)
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Measuring protein aggregation using Non-denaturing PAGE - (reply: 3)
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bacteria lysis - E coli lysis for recombinant protein extraction (reply: 1)
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Could I denature the proteins that were transferred on a membrane? - (reply: 3)
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Quantiying bands. - Determination of protein contens (reply: 2)
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Protein Sample Loading Buffer - (reply: 3)
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Ni-NTA beads and dialysis tubing - Purification using nickel beads (reply: 4)
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Tips for Western Blot of Low Molecular Weight Protein (HCV core protein) - (reply: 1)
-
shRNA shows knockdown in protein expression but no change in RT-PCR - (reply: 2)
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fusion protein construction - (reply: 1)
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gfp-tagged protein not expressing- help! - (reply: 3)
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pKa values of the protein - (reply: 1)
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Plasmids for protein expression in mammalian cells - (reply: 2)
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affinity purification and proteomics - (reply: 1)
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Native PAGE w. Western protein marker - (reply: 4)
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Concentrating Protein with centrifuge concentrator but 50% glycerol!!? - (reply: 2)
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Lyophilised protein - (reply: 3)
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Usage of EST data for non-model species in proteomics - (reply: 3)
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protein stuck - (reply: 3)
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mRNA versus protein expression. Why inverse correlation? - (reply: 3)
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A databse linking SNPs to functional consequences? - To the protein (reply: 2)
-
protein is degraded after purification - (reply: 2)
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Protein qauntitation with nanodrop - SDS and urea (reply: 1)
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Problem in protein expression - (reply: 2)
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24 well HeLa Cell Total Protein Concentration? - What would it be (reply: 2)
-
small protein blotting - (reply: 8)
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What proteins stick to ProteinG on preclearing? - (reply: 3)
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protein expression - Preparation of starter cultures (reply: 15)
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help~ how concentrate protein solution in large-scale - (reply: 2)
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Can my sample be going to the buffer instead of the membrane?? - 17kDa protein not showing in WB, transfer is fine though. (reply: 6)
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Problem with Protein elution using GST sepharose 4B - (reply: 1)
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Problem transferring HIV-1 tat protein to a PVDF membrane for western blot - (reply: 5)
-
how to isolate insoluble protein in bacterial induction? - (reply: 4)
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Protein Concentration assay (Bradford assay) - (reply: 2)
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protein digestion - (reply: 2)
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Cleaving Fusion Proteins - (reply: 1)
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In vivo monitoring leukemia growth - with green fluorescent protein or CFSE (reply: 3)
-
Acrylamide/Bis Solution for Small Protein (14 kDa) - Which Acrylamide/Bis Solution do you use for small proteins (reply: 3)
-
Working gene-coding plasmid does not produce transcript or protein - (reply: 2)
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Re-dissolving of precipitated protein?? - (reply: 1)
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Protein purification from Insect media - (reply: 1)
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sticky proteins in co-IP - (reply: 1)
-
Quality of aliquot for SDS-page in protein purification - When is pure pure enough? (reply: 1)
-
protein/DNA Sequence Alignment - (reply: 1)
-
Purifying a strange native protein - (reply: 6)
-
Protein G Sepharose beads preparation - (reply: 2)
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Ladder but no protein - (reply: 4)
-
Which test for cytokine production? - by PBMCs stimulated with proteins (reply: 6)
-
MIF protein secretion - Need help in knowing about MIF protein secretion (reply: 2)
-
Improving transient CHO protein expression - (reply: 2)
-
Changing dialysis buffer every half hour and then dialysis ON - protein precipitation prevention (reply: 1)
-
Expression of a GFP-tagged protein - (reply: 2)
-
BCA assay for reducing ends - (reply: 2)
-
BCA assay for reducing ends - (reply: 1)
-
FPLC and Protein Chromatography - Resins, FPLC and protein chromatography (reply: 11)
-
Checking For Protein Expression - (reply: 3)
-
How do I get rid of this contaminant? - His-tagged protein eluted with 300mM imidazole (reply: 13)
-
Detecting Patterns of phosphorylated protein - (reply: 1)
-
Is it possible? Harsh sonificiation conditions cause degradation of my protein - (reply: 3)
-
Low Thrombin Cleavage Efficiency Problem - I am trying to cleave a GST tagged protein(about 35 kDa + 26 kDa GST) (reply: 1)
-
insoluble protein fraction - (reply: 1)
-
Hyaluronic Acid Binding Protein 1 (HABP1) - I need some insight to prevent binding of this molecule to itself (reply: 2)
-
Newbie question: Stability of protein lystates - Freeze/thaw cycles affect phosphate groups? (reply: 2)
-
Throbin cleavage declines with older protein? - It used to work, now doesn't! (reply: 1)
-
extracting protein from trizol - (reply: 2)
-
Blue Native Gel - denaturing proteins after transfer? - (reply: 9)
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How much sonication power needed? - protein extraction from cells (reply: 2)
-
isolation of membrane associated proteins - (reply: 2)
-
Newbie question: how to determine protein concentration after IP? - (reply: 8)
-
protein extraction - from cell line in T25 flask (reply: 5)
-
Protein labeling calculation - (reply: 4)
-
Rabbit IgG purification - Rabbit IgG purification (reply: 5)
-
protein extraction using trizol - can we replace guanidine cholride with other denaturants? (reply: 1)
-
Determining a protein's antigenic property - (reply: 4)
-
Oligo annealing - phosphorylation necessary? - (reply: 4)
-
how to design a protein interaction experiment - HELP (reply: 4)
-
Desalting protein - (reply: 7)
-
Secretory protein? - How to determine if my protein is surely secreted? (reply: 2)
-
How to check the translation? - His-tagged protein expression (reply: 8)
-
protein complex and siRNA - (reply: 2)
-
protein precipitation with TCA for dna extraction? - (reply: 1)
-
G Protein Coupled Receptors - (reply: 3)
-
Protein structure discovery - (reply: 1)
-
TCA precipitation_why protein pellet disapears after centrifugation? - (reply: 2)
-
Extraction of protein from sample buffer (+DTT) - (reply: 1)
-
Protein solubilisation for iTRAQ protocol/general protein solubilisation - (reply: 2)
-
protein precipitation without phenol/chloroform for dna extraction? - (reply: 1)
-
protein aggregation problem - (reply: 3)
-
protein precipitates out during concentrating it - (reply: 2)
-
precipitation during dialysis - (reply: 5)
-
Protein glycosylation - How to determine? (reply: 2)
-
Protein stability in RIPA buffer at room temp - (reply: 3)
-
Protein synthesis inhibition - (reply: 4)
-
His tag protein purification - (reply: 3)
-
How to extract protein from SDS-PAGE gel? - (reply: 7)
-
how to put in evidence protein after IP - (reply: 2)
-
GST-fusion protein purification - How to purify it? (reply: 9)
-
protein extraction from primary cells - (reply: 1)
-
Extracting proteins from cells - (reply: 1)
-
EDTA as denaturating agent ?? - for proteins.. (reply: 5)
-
amount of proteins in urine - (reply: 15)
-
Help_Role of BSA for the protein reconstitution - (reply: 5)
-
BCA Protein Assay Problems - (reply: 1)
-
Protein lysis buffer - (reply: 4)
-
IP low protein and MS - (reply: 1)
-
protein degardation during gel filteration - (reply: 2)
-
Protein solubility improvements? - any methods? (reply: 5)
-
antibody production..does it matter if the protein is denatured or not? - my fragments are insoluble :( (reply: 3)
-
Protein Smear in SDS-PAGE after concentrated? - (reply: 1)
-
How to do Co-IP of Triton x-100 insoluble proteins (e.g. intermediate filament p - (reply: 1)
-
Immunolotting of actin protein coming at higher MW - (reply: 1)
-
Isolation of secreted proteins from culture media - (reply: 2)
-
proteins stuck at interface between stack and separating gel - (reply: 2)
-
Protein loading marker for glycoproteins - (reply: 2)
-
Trouble with protein expression in E.coli - No expression of ~ 9-11 kDa protein (reply: 2)
-
distinguish protein expression from high homology - (reply: 1)
-
Visualizing Small Peptides - Using SDS-PAGE to check for expression of small proteins (reply: 16)
-
Lost my proteins ! - (reply: 3)
-
Protein Removal from cell lysate - leaving RNA intact! (reply: 1)
-
Elution problens - My protein is not eluting in urea 8M buffer ... oh shitt!! (reply: 1)
-
Alternative methods to detect protein:protein interaction - (reply: 3)
-
protein overexpression question. - (reply: 4)
-
Protein Extraction from Human Tissue/Tumor Samples - (reply: 1)
-
Protein targetting - (reply: 1)
-
FCS and Protein assay - (reply: 3)
-
Simultaneous BLAST for several proteins - (reply: 4)
-
Problems in His-tag protein purification with TALON - (reply: 1)
-
protein quantification by bradford - (reply: 3)
-
protein extraction from tumor samples containing matrigel? - (reply: 2)
-
Extracting Proteins From Soil - (reply: 3)
-
Endogenous protein purification - (reply: 2)
-
strategy to isolate cDNA and express protein - (reply: 1)
-
Amount of protein loaded per well? - (reply: 4)
-
signal peptide search - How to find subcellular localization signals in a protein sequence (reply: 1)
-
Protein in and out of solution - (reply: 1)
-
Problems with my western blotting of LC3, autophagy protein... - (reply: 6)
-
taking protein out of membrane - (reply: 1)
-
His Tag protein - (reply: 4)
-
Contaminating proteins for Fluorescence polarization assay - (reply: 1)
-
how to dialysis against acetic acid - (reply: 1)
-
protein extraction after cell lysing - (reply: 2)
-
Protein extraction from rat aorta - (reply: 3)
-
Normal IgG background - (reply: 2)
-
Making Recombinant Proteins - (reply: 2)
-
sensitive protein quantification assay - BCA not helping (reply: 3)
-
sensitive protein quantification assay - (reply: 2)
-
GST- protein, batch purification - no protein after elution (reply: 3)
-
WB does not correspond to protein array - (reply: 1)
-
Nickel IMAC - How to avoid unwanted proteins? - (reply: 4)
-
Co-IP problem - Weird IgG band (reply: 1)
-
No protein expression - (reply: 3)
-
Purifying hydrogen bonded peripheral protein - (reply: 3)
-
How to elute this protein?! - (reply: 5)
-
Recombinant protein or peptide? - (reply: 1)
-
GST-tag protein purification - GST-tag protein purification (reply: 4)
-
strength of protein interaction for co Ip - (reply: 1)
-
Why were proteins favoured over DNA as the genetic material before 1940? - University Genetics Past EXAM Question (reply: 2)
-
Stability of Biotin labelled protein?? - (reply: 1)
-
Isolation DNA and protein from a single sample - (reply: 5)
-
How to purify a transmembrane protein from others proteins? - (reply: 1)
-
Protein structure - (reply: 1)
-
Dialysis against PBS of IgG/IgA - a bit confusing (reply: 3)
-
simultaneous extraction of nucleic acids and proteins - (reply: 3)
-
Protein tags - (reply: 2)
-
Could denatured proteins be stored at -20°C (or -80°C) until use (for SDS-PAGE)? - (reply: 3)
-
truncation of protein expression - (reply: 1)
-
SDS Page Help - using purified protein (reply: 3)
-
PROTEIN PRECIPITATION SOLUTION - (reply: 1)
-
protein precipitation - (reply: 3)
-
GST fusion protein purification - (reply: 1)
-
Integral protein question - (reply: 2)
-
storage of protein samples - (reply: 1)
-
unstable protein expression - (reply: 1)
-
DNA vs Protein Stains - Are there any that don't stain BOTH? (reply: 1)
-
splice variant encoding same protein - (reply: 1)
-
B-per GST fusion protein purification kit - (reply: 2)
-
Endogenously Biotinylated Proteins - (reply: 3)
-
protein binding site prediction - (reply: 2)
-
gradient to separate 1kDa proteins - (reply: 3)
-
Protein precipitates out so fast - (reply: 5)
-
Protein sticks to Ni beads - Protein doesn't elute from the column/batch etc (reply: 2)
-
Principles behind DNA RNA protein isolation and purification - (reply: 2)
-
Lysis Buffer for Total Protein Assay - (reply: 1)
-
Number of genes and number of proteins in humans - (reply: 1)
-
automated protein structure search - (reply: 4)
-
"Gentle" IgG elution - (reply: 1)
-
Insert phosphorylation upon Klenow? - (reply: 5)
-
Protein Conc determination - (reply: 2)
-
protein stability - (reply: 3)
-
pQE vector protein expression - (reply: 3)
-
Importance of Reducing Agent - Apparent protein size on gel (reply: 2)
-
Reconstitution of protein standard - (reply: 3)
-
Purifying protein with Neutravidin binding peptide tag - Protein purification (reply: 6)
-
protein size gradient gel - (reply: 10)
-
A story of Urea, dialysis and GST affinity purification... hopefully! - (reply: 1)
-
lyophilized protein now looks clear - (reply: 3)
-
Playing for keeps - Reusing remaining protein sample (reply: 3)
-
isoelectric point of protein - (reply: 1)
-
GST-Tagged protein - (reply: 4)
-
Effect of sonication on membrane anchored proteins - (reply: 1)
-
Methods to determine the oligomeric state of a protein - (reply: 1)
-
Problem concerning protein quantitation - (reply: 2)
-
Protein concentration - (reply: 4)
-
Getting rid of background protein binding on GST beads - (reply: 5)
-
denaturing proteins in gel - (reply: 1)
-
Protein modification search - (reply: 1)
-
Protein degradation - (reply: 2)
-
problems with detecting protein expression - protein expression in cell (reply: 4)
-
yeast two hybrid - are protein interactions species specific? (reply: 1)
-
Problem with protein precipitation - (reply: 1)
-
Software to create protein maps - (reply: 1)
-
Urea and protein storage - (reply: 6)
-
Who know anything about dialysis on protein extractions ? - (reply: 7)
-
Problem assessing protein concentration - Protein concentration (reply: 4)
-
Protein doesn't bind any column except Ni - (reply: 10)
-
Phosphoproteins staining - (reply: 2)
-
IgG control for immunopercipitation - (reply: 1)
-
quantification of Biotinylated protein ?? - (reply: 3)
-
Problems with large protein - (reply: 3)
-
protein concentration method - want really high yield (reply: 4)
-
protein structure prediction - (reply: 1)
-
help! Truncated protein cannot expressed in mammalian cells? - (reply: 6)
-
stabilization of purified protein solutions - (reply: 2)
-
Rcovery of proteins from gels - (reply: 2)
-
Protein much larger on Western blot than predicted - (reply: 12)
-
Showed a bigger and broad smear of protein!! - (reply: 4)
-
Proteins not running till the end of the gel - (reply: 2)
-
The maximum resolution obtained by crystallography? - Can we determine the primary structure of a protein by that? (reply: 3)
-
How to purify a protein (PI 8.0) using His tag? - (reply: 4)
-
mutation in secreted protein - (reply: 5)
-
Recombinant Protein migration - Doubts on protein migration (reply: 5)
-
How does the protein fixation on SDS gel work? - (reply: 3)
-
Why are my E. coli cells not pellet-able? - when expressing a chimeric recombinant protein (reply: 6)
-
SWAT for protein expression - problems with Poly Ala/GC Rich (reply: 1)
-
ChIP-CHIP IP vs IgG vs Unbound vs Input - (reply: 2)
-
Mammalian protein expression in E. coli - (reply: 1)
-
Immunoprecipitation - Multiple proteins from single lysate (reply: 2)
-
can beta-catenin be used as a house keeping protein? - (reply: 3)
-
WB for phosphorylated protein complex in rat intestinal tissue - (reply: 2)
-
Protein extraction from cell culture media - (reply: 9)
-
protein precipitation - (reply: 1)
-
brown pigment attached to the protein - (reply: 3)
-
Protein extraction from mouse organs - (reply: 6)
-
no expression of my protein - (reply: 1)
-
confirming nucleotide state of isolated G protein - (reply: 1)
-
Tagging a protein with a nucelar export signal - (reply: 2)
-
part of protein sequence missing - (reply: 4)
-
protein disapeared from gel! - (reply: 11)
-
Best storage temperature for samples - serum, protein, tissue, DNA, RNA - Archives (reply: 7)
-
Phosphoproteins - (reply: 1)
-
Mouse Urinary Protein Quantification - BCA possible (reply: 2)
-
How to define the domans in protein sequence - (reply: 3)
-
protein purification - (reply: 5)
-
protein concentration - how to determine the protein concentration very accurately?? (reply: 2)
-
IgM hybridoma protocol - Protocol targeted for isolation of IgM secreting hybridomas (reply: 5)
-
Slimy protein lysates - (reply: 3)
-
protein band size determination - any software available? (reply: 2)