Cloning strat...Awful or ok? - I use a specific strategy would love some input into how good/bad (Jul/28/2009 )

On the point of plasmid extraction from cell culture; With a little practice, I believe the standard alkaline lysis method is only slightly slower that the column binding method. The standard alkaline lysis method is superior in the amount of plasmid DNA you can extract, and it is an fair bit cheaper.

On the subject of columns, it is possible to reuse / regenerate old DNA extraction columns. This would save some money.