Data to publish. - (Mar/01/2009 )
dvddecarvalho on Mar 1 2009, 07:14 AM said:
Hey guys,
I am new on epigenetic field and I have a question.
I am working with expression of a particular gene of liver differentiation.
I found (by RT-PCR and qRT-PCR) that its expression begins during the liver differentiation and keeps steady in adult hepatocytes.
I also found (by BSP) that the DNA methylation profile of its promoter changes during differentiaton, were the CpG islands becomes severely unmethylated.
I friend of mine of epigenetic field told me that I can publish this work with only this data. The history would be something like: Expression of gene xx during liver differentiation in humans is followed by changes in the methylation profile of CpG islands on its promoter.
Do you guys think I can publish this work with such results??
Which kind of controls of BSP must I add to this work??
Your opinion would be very apreciated!
Thanks,
Dvd
I am new on epigenetic field and I have a question.
I am working with expression of a particular gene of liver differentiation.
I found (by RT-PCR and qRT-PCR) that its expression begins during the liver differentiation and keeps steady in adult hepatocytes.
I also found (by BSP) that the DNA methylation profile of its promoter changes during differentiaton, were the CpG islands becomes severely unmethylated.
I friend of mine of epigenetic field told me that I can publish this work with only this data. The history would be something like: Expression of gene xx during liver differentiation in humans is followed by changes in the methylation profile of CpG islands on its promoter.
Do you guys think I can publish this work with such results??
Which kind of controls of BSP must I add to this work??
Your opinion would be very apreciated!
Thanks,
Dvd
Have you tried In-situ PCR of methylated promoter? There are several binucleated cells in the adult liver an it isn't clear what's goes on with those cells. You may find something interesting-clarifing.
-Feelcontraire-