It's not hard to revive cells from frozen stock it just you can modify your procedure a little bit you will centifuge your cells at 1000rpm and for 2-3 minute...and then you will be able to get viable cells and you can store for longer period.
I go through the uses of biotechnology, biotechnology uses and get all my answers so i also suggest you to go through this....it is really helpful.

Regards
Rupam

Hi,
I have a problem with my Hek 293 T cells. After thawing they do not adhere to the flask. I checked if they are viable and everythig is ok.
Could be that they were too confluent when I froze them? I hit the flask for detaching the cells not to leave the too long in trypsin. is this a problem? How long can I wait untill they detach?
Thanks,

Emilia