A question about Gelanalyzer - (Jan/08/2015 )
Hi,
I have just used Gelanalyer to analyze SDS gels. I have two values that I don't know what they are and how they work. They are Cal. Volume and MW (see attached image).
Can you help me explain them. Thanks very much :)
mw is the calculated molecular weight of the band (based on rf and calibration standards).
cal. volume means calibrated volume.
you should download the manual (this is the url for the pdf).
I read, but I didn't understand how it work
Did you calibrate the molecular weights of your marker lane?
Hic5Prostate on Sat Jan 10 19:19:11 2015 said:
Did you calibrate the molecular weights of your marker lane?
My target lane is 30kDa. I have info of LWM lane
trungnghiatn on Sat Jan 10 09:11:41 2015 said:
I read, but I didn't understand how it work
you may need someone onsite to walk you through it. it's difficult to show and explain without knowing with what, specifically, you are having trouble.
trungnghiatn on Mon Jan 12 05:45:05 2015 said:
Hic5Prostate on Sat Jan 10 19:19:11 2015 said:
Did you calibrate the molecular weights of your marker lane?
My target lane is 30kDa. I have info of LWM lane
if you assign mw values to lane 1 (i assume that it is your standard) then the mws should be evaluated for the other lanes
mdfenko on Mon Jan 12 12:48:43 2015 said:
trungnghiatn on Mon Jan 12 05:45:05 2015 said:
Hic5Prostate on Sat Jan 10 19:19:11 2015 said:
Did you calibrate the molecular weights of your marker lane?
My target lane is 30kDa. I have info of LWM lane
if you assign mw values to lane 1 (i assume that it is your standard) then the mws should be evaluated for the other lanes
So, the other picks of other lanes can be figured out from MW values of LWM lane and each pick Rf,
trungnghiatn on Thu Jan 15 21:22:03 2015 said:
mdfenko on Mon Jan 12 12:48:43 2015 said:
trungnghiatn on Mon Jan 12 05:45:05 2015 said:
Hic5Prostate on Sat Jan 10 19:19:11 2015 said:
Did you calibrate the molecular weights of your marker lane?
My target lane is 30kDa. I have info of LWM lane
if you assign mw values to lane 1 (i assume that it is your standard) then the mws should be evaluated for the other lanes
So, the other picks of other lanes can be figured out from MW values of LWM lane and each pick Rf,
that's the principal. not sure with this software but with some you can average sets of standards within the gel to get more reliable results.