PCR_QUALITY CONTROL - (Dec/10/2014 )
Who to do my PCR quality control?
-laminemm-
If you mean a standard "endpoint" PCR you have several possibilities. Important ones are (which a bit depend on your experiment, too):
1. negative control (without template but water, to be sure that you're not amplifying unspecific DNAs, contaminations)
2. positive control (includes templates/mix which works surely)
3. Sequencing (to make sure that your target is really the sequence you are aiming at)
-hobglobin-
thanks you
-laminemm-