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DNA loading dye contents - (Sep/04/2014 )

I recently bought some new enzymes from NEB and they included a purple loading dye. According to the fact sheet (https://www.neb.com/products/b7024-gel-loading-dye-purple-6x), this dye runs at the same weight as bromophenol blue, but doesn't have a UV shadow. Does anyone know what this dye is for a homemade recipe?

-labstud4-

I don't know what their dye is, but orange-G works well in this application, and runs completely ahead of the DNA, so there is no shadowing issue.

-phage434-

Here is some information and recipes on some more or less common dyes and their running speeds: link

I guess NEB tries to give not any information on the (new?) dyes to avoid self-made loading buffers (and therefore to sell more) wink.png

-hobglobin-

hobglobin on Thu Sep 4 18:10:04 2014 said:

Here is some information and recipes on some more or less common dyes and their running speeds: link

I guess NEB tries to give not any information on the (new?) dyes to avoid self-made loading buffers (and therefore to sell more) wink.png

Which is a bummer because I've used their samples of this and liked it a lot more than the homemade Ficoll dye we use =\

-Bio-Lad-