dna left on thermocycler - (Aug/12/2013 )
Will anything happen to DNA I left at 30C, on with a heated lid of 105c? I forgot to add in the mastermix and placed the DNA onto the thermocycler by itself. Will anything have happened to the DNA?
-molbio1234-
For short periods, little or nothing. For day long periods, there might be some degradation if there are DNAses present, since the Mg++ and heat are there. (Actually, perhaps the Mg++ is not there, without the master mix. If your DNA was in TE, then nothing will happen.)
-phage434-
Indeed, for short periods almost nothing will happen.
-rosinha123-