2D Image Primary Cell line - (Apr/20/2013 )

the only thing that I'm suspected about my sample is that I'm using primary cell line from trachea origin in the human lung. it is epithelial cell and most probably produces strong mucous. When I'm centrifuging my cells it appears as a strong clump which is hard to re-suspend. I assume that the mucous is making this problem in strip running and focusing. I'm not sure it is right or not. If the mucous is the problem I don't know how to treat my sample.

can you wash the mucous away from the cells before you lyse them?

I m washing it with PBS but I think it may can not remove it.

you may need to add a little non-ionic detergent (not enough to disrupt the cell membrane).