How to raise polyclonal antibodies against whole bacteria live &/ or inactiv - (Jan/12/2013 )

can you please suggest protocol for raising polyclonal antibodies against whole bacteria live &/ or inactivated.

Thanks

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In which Organism will you perform your experiment? Hamster, Mouse, Rabbit etc.? Can be tricky with polyconal, am I wrong?

-Pangea-

Rabbit. I'm not familiar with protocol in hamster, mouse, but I guess more or less same principle- dose then booster at intervals.

-Inbox-

Thats just general information, but which bacteria you want to produce rabbit Ab. It will be take 3 month i guess in rabbit. Whole bacteria means just its capsule, isnt? You might look up the consistence of the capsule. It dosent matter if it Gram-negative or -positive, isnt? But i guess it will be unspecific Antibody. Do your Bacteria has specific marker molecules on his surface?

http://www.uniprot.org/keywords/875

http://ecvam.jrc.ec.europa.eu/publication/WorkshopReport35.pdf

-Pangea-

Thanks & sorry for late reply, little busy couldn't visit forum for some days. Its Salmonella typhimurium.

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