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Ethidium precipitation in CsCl Maxiprep - (Dec/02/2012 )

Hi Guys,

Anyone know what might cause the ethidium to precipitate to the bottom of the ultracentrifuge tube during a CsCl maxiprep?

It's happened repeatedly over the last few weeks, with several different cultures; there are small/no bands visible as well, despite having the expected size pellet during all of the preceding steps.

It's very strange and frustrating, any insight would be much appreciated.

Thanks!

-BChic-

Could be bound to short degraded DNA? (just an idea, don't have any real answer...)

-bob1-