Ethidium precipitation in CsCl Maxiprep - (Dec/02/2012 )
Hi Guys,
Anyone know what might cause the ethidium to precipitate to the bottom of the ultracentrifuge tube during a CsCl maxiprep?
It's happened repeatedly over the last few weeks, with several different cultures; there are small/no bands visible as well, despite having the expected size pellet during all of the preceding steps.
It's very strange and frustrating, any insight would be much appreciated.
Thanks!
-BChic-
Could be bound to short degraded DNA? (just an idea, don't have any real answer...)
-bob1-