agarose gel electrophoresis - (Sep/06/2012 )
I forgot to add ethidium bromide in the molten agrose however i added the ethidium bromide to the running buffer...Will i be able to visualise the band, if not how caqn I rescue it. kindly let me know..
with regards
yes, it should be fine.
bob1 on Thu Sep 6 08:19:13 2012 said:
yes, it should be fine.
thanks i added 5µl of ethidium bromide to the running buffer but i am tensed wheether i will be able to see it since i have to cut the band for the ligation...are you sure it will be fine.. thanks for your reply..
I used to visualize my bands after migration with an immersion of the gel in a solution with a high concentration of ethidium bromide for about 30 minutes. That works fine! In the running gel, probably would be fine but I think it depends also on the concentration of your sample. So, what is the result?
metionina on Thu Sep 6 10:20:12 2012 said:
I used to visualize my bands after migration with an immersion of the gel in a solution with a high concentration of ethidium bromide for about 30 minutes. That works fine! In the running gel, probably would be fine but I think it depends also on the concentration of your sample. So, what is the result?
It worked perfectly fine
It should do... lots and lots of people do this around the world. Actually, all you need to do is add the EtBr to the +ve electrode end, it migrates in the opposite direction to DNA so will be drawn into the gel when running.