Basis/Protocols for Choosing concentrations in testing medicinal effect plant ex - (Jan/31/2012 )

I was conducting a study to test a medicinal effect of a specific plant..I did not see any protocols on what concentration should i used. I decided to use 25%,50%,75% and 100% of the extract but still i should have a basis for using it..Anyone can help me on this...thank you so much..

I guess you would like to use a compound at a concentration comparable to its affinity or better to its EC50 to its intended target protein.

it is really depend on the method of extraction is it a simple infusion or a super critical extraction method? bellow you could find a useful guide:

http://www.unido.org/fileadmin/user_media/Publications/Pub_free/Extraction_technologies_for_medicinal_and_aromatic_plants.pdf

Cheers,
Akhshik.