Grafting - (Oct/07/2011 )
Hello!
I have a very simple question: Does somebody know if it is possible to graft a herbaceous species (such as Nicotiana) onto a woody species (such as Prunus)? It would not need to be an ever-lasting graft, it is just to transmit a virus from Nicotiana into Prunus, because the typical method of mechanical inoculation is not working and I would like to try other approaches.
If you have some other idea, I would really like to hear it!
Thank you very much and have a nice weekend!
No idea if it works, but I know that some plant viruses are transferred by "particle bombardment", i.e. mechanically by "shooting" the DNA e.g. with high air pressure into plant material. With a google search you'll find lot's of papers and protocols.
Good luck.
Thanks Hobglobin, but in this case, it is an RNA virus, and even though I have built a cDNA infective clone and I have shot the trees, it seems that it is very difficult for the virus to replicate in Prunus (and very easy to replicate in Nicotiana). That's why I was thinking of grafing.
I think I will try anyway. After all, it's a cheap method and I am quite curious!
Thanks again for the reply! 
Even if the grafting works, its still possible the infection wont work.
It depends on the Plasmodesmata, if they block the enterence of the virus then you are still in trouble.µ
Are you sure that the specific virus you use is capable of infecting that tree ?
OA17 on Fri Oct 7 14:56:52 2011 said:
Hello!
I have a very simple question: Does somebody know if it is possible to graft a herbaceous species (such as Nicotiana) onto a woody species (such as Prunus)? It would not need to be an ever-lasting graft, it is just to transmit a virus from Nicotiana into Prunus, because the typical method of mechanical inoculation is not working and I would like to try other approaches.
If you have some other idea, I would really like to hear it!
Thank you very much and have a nice weekend!
I almost forgot:
http://www.tandfonline.com/doi/abs/10.1080/07060669609500600
Check it out.
Grafting between different families/species is hard, but it can be done.
Thank you very much Pito!
I will take a look, for sure!
Hey Pito, I have searched for this paper, but it has no free access. I can't find the e-mail of the authors to ask them for a free copy or some information. Are you able to download it? In case you can, would it be a big incovenience for you to send it to me?
Thanks again!
OA17 on Thu Nov 17 13:50:55 2011 said:
Hey Pito, I have searched for this paper, but it has no free access. I can't find the e-mail of the authors to ask them for a free copy or some information. Are you able to download it? In case you can, would it be a big incovenience for you to send it to me?
Thanks again!
Here it is:
http://hotfile.com/dl/135302103/4534247/paper.pdf.html
Thanks a lot!!!! I hope it works!
I hadn't heard of grafting between different species, but if it's published I suppose it's possible. there are many different kinds of grafting. probably one that could work is a bud grafting, but then meristematic tissue is normally virus-free. I guess you'll have to try different grafting methods. good luck!