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detection of apoptosis - (May/25/2011 )

Hi

I'm looking for a way to measure the apoptosis level of human cells inside bioreactors (except LDH level)
Like with ELISA or something else that will give me a value but not microscopy techniques

any ideas?

thanks

-fadlone-

fadlone on Wed May 25 14:32:24 2011 said:


Hi

I'm looking for a way to measure the apoptosis level of human cells inside bioreactors (except LDH level)
Like with ELISA or something else that will give me a value but not microscopy techniques

any ideas?

thanks



I'm not sure if this would be helpful, but have you considered Caspase Activity Assays? We have measured caspase 3 activity level in the past with this method.

-n.t.-

what about annexin V assay using flow?

-Sajjeev Jagannathan-