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negative control for msp pcr - (May/23/2011 )

We start a new methylation specific project. Any suggestion for the best negative control DNA (unmethylated DNA), for the methylation specific pcr? It suggested that we should use placental DNA. Any alternative choice?

-sofa-

we buy unmethylated dna from milipore.

-PostDocTrauma-

Placental DNA is good. You can also try DNA extracted from sperm or whole-genome-amplified DNA (Qiagen-kit).

-MoB-