2d gel technique - (May/09/2011 )

hi, any ideas that to make sure the technique of IEF and SDS-PAGE are on the right side?
any standard or sample is recommended?

thank you

Mimosa, tenho... No Bio-rad has a solution for you: http://www.bio-rad.com/LifeScience/pdf/Bulletin_3118.pdf Check out the end of the document.

hi thank for your suggestion
any ideas about the IEF?
if the sample take longer time for focusing, what action should i take?