rRNA - (Apr/11/2011 )

Dear All,
Good day,
I wander why rRNA used as internal control, like in this article:

(Molecular cloning and characterization of two cDNAs encoding 1-deoxy-D-xylulose 5-phosphate reductoisomerase from Hevea brasiliensis)

you can see this :

(All amplifications were normalized with an 18S rRNA gene)

Why rRNA?

Thank you in advance
Noor

I suppose the 18rRNA was used as a control?
rRNA "DNA" is highly conserved (not a lot of mutations).
Maybe this is why?

(I didnt read the paper, so maybe its not why and they had a more specific) reason?

pito on Mon Apr 11 12:58:15 2011 said:

Thank you Pito for your answering,
I just need a general answer, (not specific for this article), Normally why rRNA used as a control?
And what do you mean by : (rRNA "DNA" )? Is it mean that rRNA have the same sequence for whole genomic DNA?

Thank you very much in advance

noyara on Mon Apr 11 16:00:33 2011 said:

with rRNA "DNA" ,I only wanted to say that you have to see the rRNA as DNA...oh well, never mind, thats confusing).

rRNA is very stable, it has almost no mutations so it can be used as a standard. For example it is also used in de identification of micro-organisms because its very stable.
DNA has many mutations, while rRNA not, so thats why it can be used as a standard.
(also because the start and end of the rRNA is the same, so there are universal primers, check : http://en.wikipedia.org/wiki/18S_ribosomal_RNA , this explains it a bit).


I am not sure this is the case in your paper.