How long can IF slide be stored at -20℃？ - (Jan/23/2011 )
Hi, everyone!
I just finished double IF staining of mouse brain cryo-sections several days ago. Since I will take leave for about a month, I want to store those sections at -20℃ untill I come back to work. Now I just want to ask how long can those sections be stored at -20℃ without obvious loss of flurescent intensity?
I am quite sure the intensity of fluenrescent should be OK for 1-2 weeks stored at -20℃, but how about a month?
If you have any experience on this before, why not share it with me. Thank you so much for your kindly help! 
-Flint-
They should be fine for at least a month so long as they do not dry out. Seal the edges of the coverslips with nail polish to prevent drying out.
-bob1-
bob1 on Sun Jan 23 22:33:47 2011 said:
They should be fine for at least a month so long as they do not dry out. Seal the edges of the coverslips with nail polish to prevent drying out.
Thank you so much for quick reply and helpfull informaiton.
Good luck to you!
-Flint-