Prepare p:C:I - for RNA extraction (Dec/12/2010 )
Hi,
How can I prepare: phenol: chloroform: iso_amyal alcohol --> 25:24:1, From 5:1 phenol:chloroform?
Thank you
Noor
You can't, if you really insist on the exact amounts. I suppose you could evaporate some chloroform and carefully weigh things to reduce the amount of chloroform before adding isoamyl alcohol. Or you could add a bit more phenol. But that would be stupid. Just add 2% isoamyl alcohol to get to 25:25:1 (well, not exactly, but close enough!). And, just why is this important. Usually phenol/chloroform will work just as well. Major point -- be careful working with this.
phage434 on Sun Dec 12 16:03:54 2010 said:
You can't, if you really insist on the exact amounts. I suppose you could evaporate some chloroform and carefully weigh things to reduce the amount of chloroform before adding isoamyl alcohol. Or you could add a bit more phenol. But that would be stupid. Just add 2% isoamyl alcohol to get to 25:25:1 (well, not exactly, but close enough!). And, just why is this important. Usually phenol/chloroform will work just as well. Major point -- be careful working with this.
Thank you for your suggestion.
noyara on Sun Dec 12 14:13:13 2010 said:
Hi,
How can I prepare: phenol: chloroform: iso_amyal alcohol --> 25:24:1, From 5:1 phenol:chloroform?
Thank you
Noor
add 3.8 chloroform and 0.2 isoamyl alcohol. that should give you the proper ratios.
mdfenko on Mon Dec 13 17:13:46 2010 said:
noyara on Sun Dec 12 14:13:13 2010 said:
Hi,
How can I prepare: phenol: chloroform: iso_amyal alcohol --> 25:24:1, From 5:1 phenol:chloroform?
Thank you
Noor
add 3.8 chloroform and 0.2 isoamyl alcohol. that should give you the proper ratios.
Hmm, I c, Thank you very much,
By the way, I have phenol crystal in the lab, but as I read in internet, not recommended to use crystal form, Is there any way to prepare stock solution from crystal form?
Many thanks in advance
see the cold spring harbor protocols page on phenol.