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peptide markers for native PAGE - (Nov/04/2010 )

Does anyone know of any peptide markers for native PAGE ~4kDa? I found some low range markers but they seem to be for SDS PAGE.

-donny-

you can use the peptide marker kit from ge healthcare. resuspend in water or suitable buffer instead of the sds-urea loading buffer recommended in the instructions that come with them.

-mdfenko-

Thanks for the suggestion. I saw that one too but I wasn't too sure if it would work for native PAGE since it was intended for SDS PAGE. I know some SDS-PAGE protein markers cannot be used for native PAGE, possibly due to dissociation of oligomers. Would such complications apply to the said peptide marker eg. difference in conformation?

-donny-

i haven't seen any problem using them in a native gel. you can confirm that by running 2d (native/sds) page on the markers.

most sds-page markers can't be used in native page because they have already been denatured with sds.

-mdfenko-