wrongly used HRP-conjugated antibody in IP - what kind of result can I get (Jun/28/2010 )

I did IP and got no band but heavy and light chains, and samples include a group of positive control. does that mean the antibody didnt work?
I might have wrongly used HRP-conjugated antibody instead of non-conjugated one. But if so, I think protein G would not bind this antibody anymore and there should be no band at all. Am I right?
Thanks.

I did IP and got no band but heavy and light chains, and samples include a group of positive control. does that mean the antibody didnt work?
I might have wrongly used HRP-conjugated antibody instead of non-conjugated one. But if so, I think protein G would not bind this antibody anymore and there should be no band at all. Am I right?
Thanks.

Full binding of protein G may be hindered by HRP for steric reason; additionally, unspecific binding of proteins to HRP may increase unspecific precipitations