Propodium Iodide postive and Annexin V negative - (May/07/2010 )
Hi, I am using Annexin V and propodium iodide to test the apoptosis in THP-1 cells.
There are some cells Propodium Iodide postive and Annexin V negative. Does anybody see something like this?
I believe my AnnexinV is sufficient.
-goldfinger-
goldfinger on May 7 2010, 07:09 PM said:
Hi, I am using Annexin V and propodium iodide to test the apoptosis in THP-1 cells.
There are some cells Propodium Iodide postive and Annexin V negative. Does anybody see something like this?
I believe my AnnexinV is sufficient.
There are some cells Propodium Iodide postive and Annexin V negative. Does anybody see something like this?
I believe my AnnexinV is sufficient.
PI positive cells and annexin V negative cells are supposed to be necrotic cells (unless you damaged the membranes of these cells during preparation of the samples).
-theo22-