Antibody for Fusion Protein - Antibody/ies against fusion protein (Apr/23/2010 )
Hi everyone, it may well be obvious to someone, but, when I want to target fusion proteins, do I need to search for TWO antibodies against both parts of the fusion?
For example, I have wt of protein X and Y, and I have one copy of fusion between them X`-Y`. How can I validate the quantity of both wt and fusion form?
Finally, I want to use commercially available antibodies and don`t want to raise my own.
Thanks for any advice.
best
-slav-
slav on Apr 23 2010, 11:46 AM said:
Hi everyone, it may well be obvious to someone, but, when I want to target fusion proteins, do I need to search for TWO antibodies against both parts of the fusion?
For example, I have wt of protein X and Y, and I have one copy of fusion between them X`-Y`. How can I validate the quantity of both wt and fusion form?
Finally, I want to use commercially available antibodies and don`t want to raise my own.
Thanks for any advice.
best
For example, I have wt of protein X and Y, and I have one copy of fusion between them X`-Y`. How can I validate the quantity of both wt and fusion form?
Finally, I want to use commercially available antibodies and don`t want to raise my own.
Thanks for any advice.
best
what immunological method will be used, preferentially ELISA or immunoblot?; you need two antibodies for semi-quantification; more precise is MS/MS
-Inmost sun-
I want to do Western blot, should be most suitable.
MS/MS? What do you mean? Mass spectometry?
-slav-