meaning of Absorbance units full scale - (Mar/23/2010 )

Hi people... can anyone throw light on the exact meaning of Absorbance units full scale!!! (AUFS)
is it just a unit of measure or it has some meaning to it??!!!!

Thanx!!

AUFS is a measure of the attenuation of UV through the sample - basically, if you multiply the reading you get from your sample by the AUFS, it will give you the true reading (I think, it may be the divide rather than multiply).

bob1 on Mar 24 2010, 05:01 AM said:

Thanx bob but still something seems missing here. May be i shud reframe the questions... well more like is there any difference between an OD at 214 nm in the HPLC which is measured in AU (Absorbance units) and AUFS? Are they both the same or will some kind of a factor that u are talking about plays a role here??!!!!

Thanx!!

aufs is the range of the spec or detector.

mdfenko on Mar 25 2010, 01:26 AM said:

then what does AUFS@280 nm mean??!!!!

I found this:
AUFS or ABSORBANCE UNITS FULL SCALE: refers to the attenuation of the UV detector for a particular run. A reading of 0.1 AUFS means that a 100% reading on the data system display corresponds to an absorbance of the column effluent equal to 0.1 absorbance unit. In some cases, 0.001 AUFS will be expressed as "1 milliAbsorbance Units Full Scale" (1 mAUFS).

http://www.lcresources.com/resources/getstart/a.htm

okay tat means i guess tat the Au and AUFS are essentially the same... its just a more scientific way to telling it!!!!

thanx people!!!