Storage of gel extracted DNA before ligation - (Jan/15/2010 )

Hi everyone

I'm doing cloning and was wondering whether I can store the digested (with restriction enzymes) DNA before ligation. Does stored digested DNA inhibit ligation in any way? Any help please!

The Question on Jan 15 2010, 06:49 PM said:

yeah, i have tried doing that and it woks....although, i used up the digested product within a week of preparation..............i dunno if its advisable to store for longer than that!

my friends told me keeping at -20 before ligation should be OK

Curtis on Jan 15 2010, 02:01 PM said:

Thanks a lot Veteran and Curtis.

I'll store it at -20 degree today and continue on Monday.
Thaks a lot again.