seperating cells and antibodies - Separating cells and antibodies using centrifugation (Oct/24/2009 )

Hi,

At what speed should I run a centrifuge to separate cells secreting antibodies. Is 1000g at 5 mins enough to separate cells from the antibodies

Please reply...Also if you can send me the link where this procedure is mentioned, it will be helpful

thank you

MS

-miraclestrain-

Thank you for the tips, i will work on it.

CellSpecific.com on Oct 26 2009, 03:00 PM said:

Pellet the cells by centrifugation (300xg for 5-10mins). Antibodies will be in the supernatant. Hope this helps.

-miraclestrain-