help~ how concentrate protein solution in large-scale - (Sep/15/2009 )
Hi, everyone~
I've just finished my protein expression in 2L prep, because the proteins were presented in inclusion bodies, I added denaturing buffer which contained 6M Guanidine hydrachloride to solubilize the protein. The problem was I added way too much denaturing buffer, and this made the following purification step became very complicated. I tried to concentrate the solution using diafiltration method, but it did not work, and my supervisor said I might damage the membrane already... 
Can anyone give me any suggestions? All of them are appreciated~! Thank you in advance~
-dachshund-
dachshund on Sep 15 2009, 02:28 PM said:
Hi, everyone~
I've just finished my protein expression in 2L prep, because the proteins were presented in inclusion bodies, I added denaturing buffer which contained 6M Guanidine hydrachloride to solubilize the protein. The problem was I added way too much denaturing buffer, and this made the following purification step became very complicated. I tried to concentrate the solution using diafiltration method, but it did not work, and my supervisor said I might damage the membrane already...
Can anyone give me any suggestions? All of them are appreciated~! Thank you in advance~
I've just finished my protein expression in 2L prep, because the proteins were presented in inclusion bodies, I added denaturing buffer which contained 6M Guanidine hydrachloride to solubilize the protein. The problem was I added way too much denaturing buffer, and this made the following purification step became very complicated. I tried to concentrate the solution using diafiltration method, but it did not work, and my supervisor said I might damage the membrane already...
Can anyone give me any suggestions? All of them are appreciated~! Thank you in advance~
You are in 2 ltrs? Wow. Can you bind your protein to any chromatography column? If you have a lot of ammonium sulfate, you can pellet it and then dialyze the salts out.
-BIOKMST-
BIOKMST on Sep 18 2009, 06:14 AM said:
dachshund on Sep 15 2009, 02:28 PM said:
Hi, everyone~
I've just finished my protein expression in 2L prep, because the proteins were presented in inclusion bodies, I added denaturing buffer which contained 6M Guanidine hydrachloride to solubilize the protein. The problem was I added way too much denaturing buffer, and this made the following purification step became very complicated. I tried to concentrate the solution using diafiltration method, but it did not work, and my supervisor said I might damage the membrane already...
Can anyone give me any suggestions? All of them are appreciated~! Thank you in advance~
I've just finished my protein expression in 2L prep, because the proteins were presented in inclusion bodies, I added denaturing buffer which contained 6M Guanidine hydrachloride to solubilize the protein. The problem was I added way too much denaturing buffer, and this made the following purification step became very complicated. I tried to concentrate the solution using diafiltration method, but it did not work, and my supervisor said I might damage the membrane already...
Can anyone give me any suggestions? All of them are appreciated~! Thank you in advance~
You are in 2 ltrs? Wow. Can you bind your protein to any chromatography column? If you have a lot of ammonium sulfate, you can pellet it and then dialyze the salts out.
Yes, I did 2L prep to express my isotope labeled protein for NMR analysis. My protein is His-tagged, so yes, I can purify them using Ni coated IMAC column. However, due to large amount of very diluted protein solution, I ran the whole purification for 2 weeks.... My next step is to refold the protein and desalt the solution using TFF diafiltration system~
Thanks for the post~
-dachshund-