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Methylation Related Discussions
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Bisulfite conversion, PCR end of the rope - (reply: 3)
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inconsistent and low reproducibility of bisulfite sequencing - (reply: 3)
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Problems with the study of promoters methylation - (reply: 1)
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Overcoming methylation of bacterial DNA which inhibits transformation and genome - (reply: 6)
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methylation specifc primers thermal cycle conditions - (reply: 4)
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Epigenetics Workshop - DNA Methylation Data Analysis in Leipzig, Germany (15-17 - (reply: 1)
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% Input or % methylation better for MeDIP-qPCR? - (reply: 1)
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Designing a simple assay for global DNA methylation - (reply: 6)
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why not working msp primers - (reply: 4)
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MSP Primer design - (reply: 5)
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Plasmid Methylation - (reply: 3)
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data analysis for direct sequencing BSP - (reply: 3)
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No sequencing of DNA methylation - (reply: 6)
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Best technique to analyse methylation level of CpG island - (reply: 3)
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Bisulfite modified DNA- expiration? - (reply: 1)
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KIR gene promoters for MSP primers design - (reply: 2)
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Identifying CpG islands on a promoter... - (reply: 1)
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Bisulfite conversion is complete except for the CpG sites in my non-methylated c - (reply: 5)
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methylation observation in normal cell line - (reply: 1)
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PCR of bisulfite converted DNA is now producing a smear? Previously produced a s - (reply: 2)
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bisulfite sequencing - (reply: 3)
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DNA Global Methylation with Epigentek kit-having trouble - (reply: 1)
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Nested BSP Primer Design - (reply: 6)
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Setting up bisulfite sequencing in lab - (reply: 26)
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Bisulfite/sequencing-based Methylation Analysi - (reply: 14)
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fixation and methylation - (reply: 2)
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Methylation status analysis of non CpG sites. - (reply: 6)
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Preparing overlapping bisufite sequences for methylation analysis - (reply: 2)
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AZA treatment induces the genes but bisulfite sequencing does not reveal de-meth - (reply: 11)
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How to qualitatively measure DNA methylation of gene promoter - (reply: 1)
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primer checking & restriction enzyme based methylation specific polymerase c - (reply: 8)
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does DNA methylation outside promoter region affect gene Transcription/expressio - (reply: 1)
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Determining CpG methylation and effect on gene expression - (reply: 1)
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Troubleshooting methylation primers for Bio-Rad PCR - (reply: 3)
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Bisulfite Sequencing and PCR Troubleshooting - (reply: 2)
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Methylation studies - (reply: 2)
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Sigma Imprint DNA methylation quantification assay kit - (reply: 3)
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Analysing methylation of plant promoter and enchancer - (reply: 9)
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Need Methylation MSP protocol - (reply: 3)
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designing a methylation study - (reply: 5)
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Understanding methylation for beginners - (reply: 5)
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what is the effectors of MSP? - (reply: 3)
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Issue with Xba1 and Dam methylation - (reply: 7)
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How to create a fully methylated control? - (reply: 4)
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Invitrogen Methyl Miner Tips - (reply: 2)
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DNA Methylation and genomic variation - (reply: 2)
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Primer designing for Methylation - (reply: 6)
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Bisulfite PCR and cloning - (reply: 5)
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BSP PCR primer design explained - (reply: 11)
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Bisulfite sequencing PCR worked - (reply: 5)
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Quality of Bisulfite Converted DNA - (reply: 2)
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CPG adjuvant - (reply: 1)
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How to know if observed methylation is true or a consequence of incomplete or fa - (reply: 2)
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Qiagen Epitect Bisulfite Kit - (reply: 3)
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Porportion methylation of bisulfite DNA changes with time? - (reply: 4)
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BSP testing for differences in methylation pattern by use of melt curve before s - (reply: 1)
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Q regarding primer mix for MSP!! - (reply: 4)
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suitable DNA extraction and sample freezing for MSP - (reply: 1)
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BSP Primers with M13 Tails - (reply: 1)
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cytosine methylation stability - (reply: 1)
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About Methylated & Non-methylated pUC19 DNA Set - (reply: 1)
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Bisulfite PCR Question - (reply: 2)
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Bisulfite PCR doesn't work and the primer seems abnormal - (reply: 6)
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Bisulfite Primers- "Top" and "Bottom" Strand - (reply: 5)
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no DNA fragment pull down in the first CpG island (MBD-seq) - (reply: 2)
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designing methylation specific primers - (reply: 3)
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Effect of varying DNA concentration levels in methylation analysis - (reply: 2)
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Different primer optimalization for nested vs direct MSP? - (reply: 3)
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Bisulfite conversion conundrum - (reply: 8)
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Help! No bands after BSP! - (reply: 3)
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MSP primers- finding the product length - (reply: 4)
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Can we use degenerate bases in BSP primers? - (reply: 1)
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Can anyone help with my Bisulfite PCR? - (reply: 2)
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Plasmid clone in BSP - (reply: 1)
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Sequencing of the PCR production in BSP - (reply: 1)
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Taq polymerase for MSP - (reply: 1)
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RUNX3 Methylation specific PCR not working-please help - (reply: 8)
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Spin columns with bisulfite conversion - (reply: 1)
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Problem of Direct bisulfite sequencing - (reply: 1)
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Bisulfite cloning... reading material - (reply: 1)
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DNA in methylation analysis - (reply: 3)
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MSP Primers not working - (reply: 6)
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plotting DNA methylation profile vs TSS - (reply: 1)
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Methylation sensitive enzymes - (reply: 1)
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problems with Bisulfite PCR - (reply: 1)
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In search of universal and reliable controls for methylation analyses - (reply: 1)
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PLATINUM TAQ FOR MSP? - (reply: 2)
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Checking my own Methylated Control for MSP - (reply: 1)
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Direct sequencing - BSP - (reply: 1)
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methylation analysis with MS-HRM (primer design) - (reply: 1)
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Methyl Binding Protein-1 (MBD1) / Immunofluorescence - (reply: 2)
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DNA methylation of a promoter region in different age groups - (reply: 2)
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statistical analysis or quantitative analysis method for bisulfite sequencing r - (reply: 4)
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Advice on optimising bisulfite PCR sought - (reply: 2)
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checking msp primers - (reply: 2)
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"CpG methylation: Blocked by some combinations of overlapping" - (reply: 1)
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To isolate DNA, RNA and Protein from same culture flask for methylation study? - (reply: 2)
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Bisulfite treatment of DNA follow by Sequencing - (reply: 2)
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MSP U primers very difficult to amplify... - (reply: 4)
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Direct sequencing PCR product - for bisulfite sequencing (reply: 3)
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effect of DNA isolation and storage on methylation - (reply: 1)
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which is the best plasmid vector for cloning BSP products? - (reply: 2)
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Are my MSP primers good? - MSP primers design by Methyl Primer Express v1.0 (reply: 2)
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Measuring methylation on X chromosome of female subjects - How can I overcome artifacts from X linked inactivation in females? (reply: 3)
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High throughput methylation mapping - Infinium or other? - (reply: 3)
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Methods of Methylation detection not requiring bisulfite treatmetn - (reply: 2)
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negative control for msp pcr - (reply: 2)
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How to blast methylation specific and unspecific primers - (reply: 1)
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Problems designing primers for BSP - (reply: 2)
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How is bisulfite converted DNA unstable over time? - (reply: 4)
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Putative tumor suppressor promoter heavily methylated yet upregulated by DNA dam - (reply: 3)
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problems with MS-HRM - late detection of PCR signal - high resolution melting, methylation (reply: 2)
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RNA carrier when using not so good DNA for bisulfite conversion - (reply: 3)
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Absence of CpG islands - (reply: 1)
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MSP - (reply: 10)
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How rapid are DNA methylation changes? - (reply: 1)
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Methylation study-the most opted study - (reply: 3)
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Drop down during bisulfite sequencing - (reply: 2)
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Standardization of HRM for methylation analysis - (reply: 4)
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Question: Start with genomic DNA or mRNA for methylation analysis - (reply: 4)
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Bisulfite sequencing primer design query (probably simple) - (reply: 4)
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Inter assay variation in global methylation Elisa - (reply: 2)
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designing MSP primers (dimers NOOOOOOOO!) - (reply: 1)
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Are my bisulfite treated sequences methylated or not?? - (reply: 2)
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DNA and histone methylation help - (reply: 1)
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Can every gene be methylated? - (reply: 1)
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Help about BSP question. - (reply: 2)
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Amplification of bisulfite converted gDNA for sequencing - (reply: 1)
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pcr / primer theory in bisulfite sequencing - (reply: 4)
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DNA yields after Bisulfite Treatment - How can I optimize when I have low yield? (reply: 16)
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Problem with Qiagen EpiTect Bisulfite Kit - (reply: 2)
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Feel really stupid for asking this ...Bisulfite Sequencing, help please! - (reply: 10)
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Bisulfite seqencing - Bisulfite seqencing gets unacceptable seqence (reply: 2)
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PCR amplification before bisulfite conversion - (reply: 6)
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Methylation and luciferase reporter query - (reply: 4)
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Bisulfite conversion kits - (reply: 6)
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Digestion problem XbaI, methylation sensitive site - (reply: 3)
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SNP and DNA methylation - (reply: 1)
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Bisulfite sequencing is giving problem - (reply: 7)
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methylation specific PCR - Primers - (reply: 1)
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Tips for MSP ... - (reply: 4)
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Standarization of HRM for methylation - (reply: 4)
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Whole Genome Amplification of bisulfite treated DNA - (reply: 7)
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no methylation vs full methylation - (reply: 3)
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Bisulfite sequencing - (reply: 3)
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Finding bisulfite PCR primer sequence - (reply: 3)
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No amplification after bisulfite treatment - (reply: 4)
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problems when use qPCR to quantify bisulfite modified DNA - (reply: 4)
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Primer Design MSP, BSP, MS-HRM - (reply: 3)
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Bisulfite sequencing not working! - (reply: 4)
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MSP - unwanted amplification - (reply: 5)
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Smears in Bisulfite seq. PCR - (reply: 3)
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Multiple bands after bisulfite PCR - (reply: 7)
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Supposedly etablished MSP not working - E-Cadherin promotor (reply: 9)
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tough problems on methylation study - (reply: 4)
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How to reduce the bisulfite PCR bias? - (reply: 4)
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Regarding methylation and luciferase assay - Regarding methylation and luciferase assay (reply: 4)
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Global methylation assay - kit? - (reply: 7)
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Help with data from directly sequencing BSP product... - (reply: 2)
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BSP primers design, help please - (reply: 2)
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Bisulfite treatment - (reply: 7)
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Bisulfite sequencing PCR not working - (reply: 5)
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Bisulfite Conversion Efficiency - (reply: 1)
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Best method for validating DNA methylation array data - (reply: 1)
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promoter methylation data analysis - (reply: 3)
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bisulfite sequencing cloning problem (methylation of DNa) - bisulfite sequencing cloning problem (methylation of DNa) (reply: 3)
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MSP primer troubles - (reply: 3)
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Gene expression of methylated gene - (reply: 4)
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Measuring global methylation using real time PCR - (reply: 2)
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primer for bisulfite sequencing in a known unmethylated region - is it safe? - (reply: 2)
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free bisulfite conversion kit - (reply: 2)
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are plasmids used for lab work methylated - (reply: 1)
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Zymo EZ Methylation Gold DNA bisulphite treatment - No DNA present after treatment (reply: 5)
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Methylation analysis in vitro - (reply: 3)
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dam methylated DNA in dam - straights - (reply: 1)
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How to define CpG islands and what to do if they aren't found? - (reply: 3)
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All cytosines are methylated!! - (reply: 4)
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Polymerases voor bisulfite sequencing - any fancy new enzymes? (reply: 4)
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multiplex methylation analysis (bs conversion based) - (reply: 1)
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Methylation specific DMSO PCR - (reply: 1)
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Inconsistent MSP results - (reply: 7)
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"Quick and Dirty" methylation status estimates - Restriction profiling of DNAnmethylation (reply: 4)
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Comparison of Bisulfite conversion kits - (reply: 7)
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Site Directed methylation - (reply: 1)
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Amount of positive control for bisulfite treatment - (reply: 1)
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Bisulfite Sequencing PCR help! - BSP is Failing like Gangbusters. Please help! (reply: 6)
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genome methylation analysis/database - analyze all promoters for CpG islands (reply: 1)
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Problem cloning bisulfite PCR BSP product - (reply: 6)
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DNA methylation analysis - direct sequencing gives CT peaks - (reply: 1)
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Whole Genome Methylation Analysis Feasibility - Methylation analysis on DNA from rare cell populations (reply: 3)
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Methylation Questions - Some basic questions about methylation (reply: 2)
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heredity of DNA methylation - (reply: 2)
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global DNA methylation quiagen kit - (reply: 14)
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Measuring global methylation by QPCR - (reply: 1)
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Manual reverse primer design for bisulfite traeted DNA - (reply: 4)
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sequencing clones after BSP : need miniprep ?? - (reply: 1)
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DNA methylated PCR - (reply: 2)
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Product length for BSP - (reply: 4)
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Bisulfite vs metabisulfite - (reply: 3)
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BSP PCR for help - no bands of tissue amplification (reply: 3)
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Methylation specific PCR - Methylation specific PCR - problem with controls.... (reply: 2)
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weak bands for methylated DNA - (reply: 4)
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How to present bisulfite seq data - with CpGviewer (reply: 7)
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BS PCR..please help - DNA methylation analysis (reply: 15)
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BSP PCR standardization...help - (reply: 8)
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DNA Enrichment for Methylation Analysis - (reply: 8)
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Perplexing Bisulfite Sequencing Problem - (reply: 3)
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Help me! How to design nested BSP primers? - (reply: 4)
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length of bisulfite sequencing reads on a ABI - my reads are not so long!!! (reply: 2)
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CpG Complementary Symmetry - (reply: 2)
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Strange results from bisulfite sequencing - (reply: 2)
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Integrity of treated DNA - Is quality of bisulfite treated DNA poor? (reply: 1)
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5-AZA, Reversing DNA methylation (epigenetic reactivation experiments) - (reply: 3)
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Sybre green taq man PCR assay for quantitative methylation detection - (reply: 2)
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Imcomplete bisulfite treatment - (reply: 2)
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Bisulfite Sequencing PCR - (reply: 4)
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Restriction Enzyme Digest of Genomic DNA - Problem with RE-digest + PCR in CpG island assay (reply: 1)
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Is gDNA digestion before bisulfite conversion important? - (reply: 5)
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Bisulfite PCR Inconsistent, often smears - (reply: 4)
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Direct sequencing of BSP product - (reply: 3)
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Preparing Methyl Cellulose - (reply: 4)
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BSP primers - (reply: 2)
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Anybody knows a good histone methylation inhibitor? - (reply: 2)
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MSP Score - (reply: 1)
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DNA methylation prediction in plant DNA - (reply: 2)
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methylation erased by pcr: how and why - why does PCR erase methylation information? (reply: 7)
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Questions regarding bisulfite treatment of DNA - (reply: 2)
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Bisulfite genomic sequencing PCR - PCR conditions (reply: 7)
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comparison of bisulfite sequencing and base specific cleavage coupled with Mass - (reply: 5)
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How stable is methylated DNA? - (reply: 4)
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Suitable sample for promoter methylation screening - (reply: 4)
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Analysis of direct bisulfite sequencing data - (reply: 9)
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MethylCode Bisulfite Conversion Kit from Invitrogen - Used this kit? (reply: 4)
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EZ DNA Methylation kit - (reply: 1)
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polymerase for BSP - (reply: 2)
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Designing and analyzing BSP - (reply: 1)
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BSP,MSP primer design? - (reply: 1)
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BISULFITE CONVERTION ?? TROUBLE SHOOTING - (reply: 4)
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MSP: No PCR product for one cell line but yes for the other cell lines - (reply: 3)
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GAPDH methylated or not - (reply: 2)
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How adjacent or remote CpG affects expression? - (reply: 5)
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positive control for BSP? - (reply: 12)
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Heterogeneous methylation in a same cell line - (reply: 1)
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Confirm DNA enrichment of methylation array - (reply: 2)
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modifications in bisulfite treatment - (reply: 6)
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MSP problem - (reply: 7)
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western for methylated histone - (reply: 7)
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How to design prime detecting plant methylation? - (reply: 3)
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Negative control problem in BSP - (reply: 1)
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positive control for MSP - (reply: 6)
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Generating primers for MSP - (reply: 1)
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Zymo CpG Methylase (M.SssI) Kit - Alternative Distributor to NEB (reply: 1)
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MSP SENSITIVITY - (reply: 1)
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methylation by MSSS1(NEB )for reporter gene analysis - (reply: 2)
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Does phenol extraction have effect on DNA methylation? - (reply: 4)
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Effect of freezing on DNA methylation? - (reply: 6)