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Membrane Related Discussions
  1. PVDF Membrane Floating in Reprobe Solution . . . - (reply: 5)
  2. Semipermeable membranes - (reply: 5)
  3. Semipermeable membranes - (reply: 7)
  4. Cell Membranes as an active barrier - (reply: 1)
  5. Percentage of Outer Membrane Protein - (reply: 2)
  6. Outer membrane protein extraction - (reply: 1)
  7. Outer membrane protein extraction - (reply: 5)
  8. Express two different membrane proteins on the same promoter - (reply: 1)
  9. Storing membrane after antibody incubation - (reply: 2)
  10. Can I incubate two membranes in one container? - (reply: 2)
  11. Human cell membrane protein Western blot! - (reply: 2)
  12. Can propidium iodide be used on WBC nuclei with cell membrane removed - (reply: 1)
  13. Blocking and Washing membrane - (reply: 3)
  14. Does ultrasonic probe sonication break up mitochondria or nuclear membrane? - (reply: 2)
  15. Does TritonX-100 lysis buffer break up mitochondrial membrane? - (reply: 2)
  16. Does Ultrasonic probe sonication break either mitochondrial or nucler membrane? - (reply: 3)
  17. I stored my PVDF membrane at 4C ? - (reply: 2)
  18. Is it possible to "over-block" a membrane? - (reply: 5)
  19. storing membrane - (reply: 2)
  20. Methods to quantify human GLUT4 translocation to plamsa membrane - (reply: 1)
  21. Choosing MWCO For Dialysis Membrane - (reply: 3)
  22. fluorescent tag on membrane protein - (reply: 2)
  23. membrane protein expression - (reply: 2)
  24. Incomplete Blotting and staining of NC membrane - (reply: 7)
  25. genomic DNA extraction on silica-based membrane - (reply: 1)
  26. Breaking nuclear membrane with needle is sufficient (no sonicator nor dounce)? - (reply: 2)
  27. What is the membrane made of in DNA purification kits - (reply: 3)
  28. Problems with western membrane stripping - (reply: 2)
  29. PVDF membrane long-term storage after transfer - (reply: 3)
  30. LMW proteins not transfer of gel to membrane - (reply: 1)
  31. Biotinylated RNA on Nylon Membrane - (reply: 2)
  32. Membrane protein - (reply: 1)
  33. Undergrad "activated" a NS membrane with 100% MeOH... Any hope? - (reply: 5)
  34. Mix of Milk, BSA, Casein for membrane blocking - (reply: 3)
  35. Membrane protein extraction - (reply: 2)
  36. Membrane Protein Extraction - (reply: 2)
  37. membrane proteomics - (reply: 1)
  38. Membrane proteins on 2D gel electrophoresis..... - (reply: 1)
  39. Membrane vs. Cytosol Fractionation - (reply: 2)
  40. Stain nitrocellulose membrane with coomassie stain? - (reply: 3)
  41. Ponceau red problem: membrane turned red - (reply: 3)
  42. Principle of silica membrane binding of nucleic acid - (reply: 6)
  43. membrane proteins ultrafiltration - (reply: 2)
  44. Membrane protein concentration - (reply: 1)
  45. Protein concentration of a labelled membrane protein - (reply: 2)
  46. The pitfalls of Membrane Preps - (reply: 1)
  47. Membrane Protein SDS PAGE and WB - (reply: 1)
  48. SDS solubilisation, Membrane Proteins and Micelle Formation - (reply: 2)
  49. Immuno-staining for cell surface/membrane markers after permeabilisation - (reply: 4)
  50. Stripping the membrane for LiCor - (reply: 1)
  51. Mass-spectrometry of membrane proteins - (reply: 1)
  52. Western blot membrane imaging - (reply: 7)
  53. Storage of Membrane before addition of primary antibody - (reply: 3)
  54. mass spectrometry and tryptic digestion on membrane proteins - (reply: 5)
  55. PVDF membrane silver staining protocol - (reply: 5)
  56. Why can't we detect multiple protein in a single membrane using western blot - (reply: 2)
  57. Reprobing a PVDF membrane which has gone dry - (reply: 1)
  58. Secondary Ab is M.I.A. ... how long can I leave my membrane in wash buffer? - (reply: 1)
  59. Ponceau on PVDF membranes affecting immunoprecipitation antibody staining?? - (reply: 3)
  60. problems with housekeeping antibodies when re-probing membranes - (reply: 3)
  61. How to store a membrane? - (reply: 1)
  62. membrane protein expression - (reply: 1)
  63. RNA transfer to nitrocellulose membrane (Northern blot) - (reply: 2)
  64. 2-D electrophoresis of sperm membrane extracts - (reply: 9)
  65. Smeared transfer on nitrocellulose membrane - (reply: 2)
  66. Help on using different antibodies in the same membrane - (reply: 8)
  67. Stripping buffer for nitrocellulose membrane - (reply: 1)
  68. BN-PAGE of purified membrane associated protein - (reply: 5)
  69. Re-using membranes with ghost bands - (reply: 2)
  70. Dot Blot - Protein not adhering to membrane - (reply: 1)
  71. Membrane proteins - (reply: 6)
  72. Transfer not occurring on right hand side of membrane correctly - (reply: 3)
  73. Areas of membrane bleached - (reply: 1)
  74. Dot-blot with immobilized ABs on membrane for protein detection - (reply: 8)
  75. membrane filters for chromatography buffers - (reply: 1)
  76. ELISA plate coating with plasma membrane vesicles - (reply: 1)
  77. HELP!!! Non-specific binding on my membrane - (reply: 7)
  78. Help! Half membrane exposed perfectly. Half membrane is cloudy - (reply: 2)
  79. Imaging western blot membrane - (reply: 2)
  80. Problem of recombinant bacterial membrane anchored protein - Recombinant protein (reply: 2)
  81. Membrane solubilisation - (reply: 2)
  82. protocol to transfer protein on transfer membrane fluoride bind - please tell me how to transfer proteins from sds page (reply: 4)
  83. What causes RNA/DNA to unbind from silica membrane? - (reply: 1)
  84. Nitrocellulose membrane storage after transfer - (reply: 6)
  85. HELP!?!? with MEMBRANE PROTEIN extraction - Extraction of GPCRs from HEK293T for IB and IP (reply: 1)
  86. Identification of Membrane Receptor protein, how ?? - I wanna use MS approach ! (reply: 4)
  87. Membrane fluidity by flow cytometry - (reply: 3)
  88. Having trouble with stripping PVDF membrane during WesterDot blot - (reply: 1)
  89. PVDF membrane in direct contact with kodak film - PVDF membrane accidentally touching kodak membrane (reply: 1)
  90. Filming membrane (Western Blot & ECL) - (reply: 1)
  91. Homogenization for Plasma Membrane Protein Extraction - (reply: 1)
  92. Molecular Cell Biology - Trafficking and function of a membrane enzyme (reply: 1)
  93. Dry or not PVDF membrane after transfer - (reply: 2)
  94. Western Blots on Membrane Proteins - (reply: 9)
  95. Cloning strategy for a membrane protein - did I do anything wrongly? (reply: 2)
  96. kit fpr cell membrane preparation? - (reply: 2)
  97. total cell protein vs membrane protein - (reply: 4)
  98. No signal on dried PVDF membranes after MeOH rehydration/stripping/reprobing - (reply: 3)
  99. PVDF membrane dried after transfer (Western) - (reply: 6)
  100. Which side of the membrane should face up during incubation steps? - Does it matter? (reply: 4)
  101. reprobing a membrane to detect a protein with the same molecular weight - (reply: 4)
  102. How to store wet dialysis membranes once opened? - (reply: 4)
  103. Cellulose Membranes - Problem with my membrane (reply: 3)
  104. Re-use membrane in Western Blot - (reply: 3)
  105. NP40 or Triton as membrane protein detergent - (reply: 2)
  106. Analyzing outer mitochondrial membrane proteins via WB - (reply: 6)
  107. Brown PVDF membrane after rehydration - (reply: 5)
  108. membrane protein aggregation - (reply: 1)
  109. problem of membrane protein FPLC - (reply: 1)
  110. my western blots of membrane protein do not work - (reply: 9)
  111. Membrane Solubilize with CHAPSO - Insoluble fraction (reply: 1)
  112. buffer for membrane protein - (reply: 1)
  113. membrane protein purification - (reply: 6)
  114. membrane type of cell culture filtration - (reply: 3)
  115. membrane damaged = cell death? - (reply: 2)
  116. Bands visible in membrane but not in the film - ?? (reply: 10)
  117. Permeabilize Eukaryotic Membranes - (reply: 11)
  118. MEthanol in NC membrane - (reply: 7)
  119. reconstitution of membrane proteins into liposomes - (reply: 1)
  120. Membrane dried after secondary antibody - can I still develop color? (reply: 6)
  121. Membrane protein isolation - (reply: 1)
  122. membrane protein extraction buffer - (reply: 1)
  123. Could I denature the proteins that were transferred on a membrane? - (reply: 3)
  124. I need help with the membrane fatty acid determination - (reply: 9)
  125. Can my sample be going to the buffer instead of the membrane?? - 17kDa protein not showing in WB, transfer is fine though. (reply: 6)
  126. Problem transferring HIV-1 tat protein to a PVDF membrane for western blot - (reply: 5)
  127. Mitochondrial membrane potential gain - (reply: 1)
  128. Mitochondrial membrane potential - (reply: 3)
  129. Outer membrane preparation protocol. - (reply: 1)
  130. my membranes are blue! - silly mistake, what to do? (reply: 1)
  131. isolation of membrane associated proteins - (reply: 2)
  132. Beginner Question: Membrane position during Blocking and washing? - (reply: 1)
  133. How to store membrane with primary antibody? - (reply: 5)
  134. taking protein out of membrane - (reply: 1)
  135. Big white spots on PVDF membrane - (reply: 1)
  136. nylon membranes bleaching - (reply: 2)
  137. Cutting membrane after transfer - (reply: 27)
  138. Effect of sonication on membrane anchored proteins - (reply: 1)
  139. resuspend membrane pellet - (reply: 2)
  140. resuspend membrane pellet - (reply: 3)
  141. HELP URGENT! FLUID MOSAIC / CELL MEMBRANE - (reply: 2)
  142. Detergent concentration for nuclear membrane dissolving - (reply: 2)