|
|
Electrophoresis Related Discussions
-
Finding the mass of digested DNA by electrophoresis - (reply: 1)
-
Pregnant - using Ethidium Bromide & UV for gel extraction - (reply: 1)
-
What casues dark zone in middle of DNA band on agarose gel? - (reply: 7)
-
Electrophoresis of proteins from cells - (reply: 9)
-
Problems for DNA agarose gel visualization with a gel documentation system - (reply: 9)
-
RNA degradation from cells embedded in gels - (reply: 4)
-
SDS-PAGE/2D gel and western blot for multiple proteins for multiple sample - (reply: 1)
-
Hae III preparation for agarose gel electrophoresis - (reply: 2)
-
SDS PAGE electrophoresis - (reply: 1)
-
Why do I get two bands on the gel of a digested PCR product after purification w - (reply: 1)
-
Weird agarose melting at standard conditions - just at the wells?? - (reply: 1)
-
How to add prepare protein sample for loading into the gel? - (reply: 3)
-
Problem with RNA bands on RNA bleach gel - (reply: 1)
-
Need help with my SDS-PAGE gels - (reply: 9)
-
Agarose beads IP troubles - (reply: 7)
-
Very low yields for >10kb fragment gel extractions - (reply: 2)
-
protein purification from agarose beads using immunoprecipation kit - (reply: 2)
-
Dye does not separe in acrylamide gel - (reply: 2)
-
Why is the smearing becoming different in the polyacrylamide gel than the agaros - (reply: 2)
-
5X, 6X sds gel loading buffer - (reply: 4)
-
SDS PAGE gel length effect on resolution - (reply: 1)
-
Need to run gel after miniprep before digest? - (reply: 1)
-
lower part of the gel was not stained - (reply: 7)
-
Gel Electrophoresis: Gel Poured With Water - (reply: 2)
-
UV-Imaging Chambers: Oversaturation from many gel lanes? - (reply: 1)
-
Use of fluorescent stain on IEF gels - (reply: 1)
-
doublets on 2D gel - (reply: 4)
-
layer in between resolving and stacking gel - (reply: 1)
-
continual problems with 2d gel electrophoresis - (reply: 3)
-
PCR bands on gel - (reply: 5)
-
RNA agarose gel blurry fuzzy. RNase contaminaton? - (reply: 2)
-
Electrophoresis problem: Frowning bands and smeary ladder - (reply: 1)
-
Insanely difficult time trying to cast large FACE gel for PROTEAN XI system - (reply: 1)
-
Agarose Gel consistency issues - (reply: 2)
-
Bubbles in western blot gel "AFTER" running gel - (reply: 2)
-
Using SDS for DNA electrophoresis - (reply: 3)
-
Migration of circular DNA vs circular/crosslinked peptide in gels - (reply: 2)
-
Solubilizing agarose in soft agar assay for absorbance reading - (reply: 1)
-
Protein not migrating through gel - (reply: 9)
-
RNA electrophoresis - (reply: 2)
-
Gel Purification: When is it safe to pause? - (reply: 2)
-
How to extract Biotinylated DNA from low melting agarose without use of phenol e - (reply: 1)
-
Agarose gel problems - shiy spots on gel - (reply: 1)
-
visualization of small peptide on tris-ricine sds page gel - (reply: 2)
-
Deformed bands in gel - (reply: 8)
-
Proper agarose for bacteria colony? - (reply: 4)
-
Transfer buffer for tris tricine gel - (reply: 12)
-
Protein stuck in the origin of resolving gel - (reply: 12)
-
detection of small peptide by the SDS-PAGE gel - (reply: 1)
-
Agarose Gel Electrophoresis - (reply: 1)
-
How to determine gene knock-out from Agarose gel bands - (reply: 1)
-
Question on Agarose Plate Assay Protocol - (reply: 1)
-
What would be the adequate %gel/voltage/running time conditions for a ca. 320 kD - (reply: 3)
-
Mystery of SDS gel, working with the protein GB1 (T2Q mutation) - (reply: 3)
-
Colour of PCR gel band best for ImageJ analysis - (reply: 2)
-
Syber green gel staining after electrophoresis - (reply: 4)
-
Agarose gel for determining RNA quality (image attached) - (reply: 3)
-
Visualization of DNA:RNA hybrid on TBE-UREA GEL - (reply: 8)
-
Gel doubling banding and resolution problem - (reply: 6)
-
PCR DNA bands too large on agarose gel - (reply: 1)
-
2D gel analysis software - (reply: 1)
-
All bands, including ladder, appear as double bands in EtBr stained gel - (reply: 6)
-
RNA gel problem (melted bands) - (reply: 2)
-
Layer of water above separating gel mixes into gel - (reply: 2)
-
quiaquick gel extraction troubleshooting - (reply: 1)
-
Gel filtration of COX-1 by aspirin - (reply: 1)
-
Capillary electrophoresis separation - (reply: 2)
-
Staining SDS-PAGE gel with EtBr? - (reply: 6)
-
Smeary bands in gel electrophoresis - (reply: 3)
-
DNA SDS-PAGE electrophoresis - (reply: 6)
-
Problems with SgrAI digest, ligation, killer cut and weird bands on gel - (reply: 4)
-
Help on native PAGE gel and its western blot result - (reply: 5)
-
agarose electrophoresis gel has 500 bp bright smear after mini prep - (reply: 1)
-
Band did not migrate on gel electrophoresis (SDS) - (reply: 3)
-
Help!!! Agarose Gel - (reply: 2)
-
DNA shows on Nanodrop but not on electrophoresis - (reply: 2)
-
RNA Gel 3 Sharp bands? - (reply: 1)
-
Gel Bands - (reply: 1)
-
Agarose gel electrophoresis uneven migration - (reply: 8)
-
Gel Electrophoresis and RNA - (reply: 1)
-
several bands in non-reducing SDS-page gel - protein without cysteines - (reply: 2)
-
Vortexing agarose beads - (reply: 3)
-
DIY PCR Cleanup/Gel extraction and miniprep solutions - (reply: 2)
-
Problem with 15% SDS page gel during electrophoresis - (reply: 7)
-
Gel Extraction Column [Orange Stain during first spin] - (reply: 2)
-
haeting RNA before electrophoresis - (reply: 1)
-
PCR Products not run well in the GEL - (reply: 1)
-
Gel for my ligation - (reply: 1)
-
first time running DNA polyacrylamide TBE gel - (reply: 4)
-
DNA gel elctrophoresis problem - (reply: 3)
-
Relative humidity in the electrophoresis lab - (reply: 3)
-
Ladder and proteins do not migrate into stacking gel - (reply: 5)
-
Transforming dna from agarose gel - (reply: 3)
-
page electrophoresis - (reply: 2)
-
Low A260/230 after gel extraction - (reply: 1)
-
Disparity between nanodrop and gel - (reply: 2)
-
RNA agarose gel electrophoresis came up empty - (reply: 10)
-
Sequencing gels equipment query - (reply: 2)
-
Measuring pcr fragments in a gel - (reply: 1)
-
gel electrophoresis - (reply: 4)
-
Top of agarose gel blank? - (reply: 3)
-
Viral Proteins Are Not Migrating Down the Gel - (reply: 10)
-
EtBr decontamination of gel doc system - (reply: 2)
-
Electrophoresis Imaging - (reply: 1)
-
Electrophoresis after PCR : too many bands - (reply: 6)
-
DNA virus purification from agarose failed! - (reply: 7)
-
Ligation failure? I ran some on a gel - (reply: 6)
-
Why does my gel have streaks instead of bands? - (reply: 2)
-
Can I use an EtBr stained PAGE gel for northern blot? - (reply: 2)
-
what do you think is the problem with this polyacrylamide gel? - (reply: 2)
-
How to improve yield in DNA-purification from agars electrophoresis? - (reply: 3)
-
Electrophoresis samples and volumes - (reply: 1)
-
Native PAGE problem - not entering gel enough - (reply: 9)
-
PCR with no bands showing in 1.1 % gel electrophoresis - (reply: 4)
-
Problems with wells on SDS PAGE gels - (reply: 3)
-
Checking RNA integrity by Denaturing Gel - (reply: 2)
-
Gel Electrophoresis: Help needed - (reply: 15)
-
Altering gel voltage and run time. - (reply: 9)
-
BrdU incorporated DNA- move slower during electrophoresis? - (reply: 2)
-
3D printing of (horizontal, agarose) gel trays, and other lab equipment - (reply: 6)
-
DNA Gel purification column issues/mistakes? What's your process? - (reply: 6)
-
polyacrylamide gel with sybr green staining - (reply: 1)
-
Weirdest thing with DNA dye and agarose gel - (reply: 4)
-
PAGE loading dye vs agarose loading dye - (reply: 1)
-
Is NuPAGE Bis-Tris gel for native or denaturing protein? - (reply: 1)
-
Need help with dCAPS pcr, seeing huge bands on gel - (reply: 1)
-
Smeary bands on agarose gel (also the ladder) - (reply: 2)
-
manual 2D electrophoresis - (reply: 8)
-
Is there any alternative to Formamide in RNA Gel Electrophoresis? - (reply: 1)
-
Sudden problems with Separating Proteins using SDS Gel - (reply: 4)
-
Multiple Bands after gel extraction - (reply: 4)
-
PCR Purification or Gel Extraction for Southern Blot - (reply: 5)
-
UV analysis of gel - (reply: 2)
-
DNA extraction from the gel - low yield - (reply: 2)
-
Plasmid digestion and gel purification using HindIII - (reply: 3)
-
agarose sterilization - (reply: 3)
-
Agarose bead size in affinity chromatography - (reply: 2)
-
Dotted bands on my DNA agarose electrophoresis gel. - (reply: 2)
-
Perculiar contamination of agarose gel combs? - (reply: 3)
-
MS-compatible methods for blocking agarose beads - (reply: 1)
-
2D gel analysis software - (reply: 1)
-
Gel purification (DNA) - (reply: 1)
-
Running Plasmid DNA on Agarose Gel (Banding Pattern) - (reply: 1)
-
Running undigested plasmids on gel - (reply: 2)
-
Diffuse rRNA bands on RNA denaturing (formaldehyde) gel - (reply: 5)
-
Coomassie gel after semi-dry transfer - normal like this? - (reply: 7)
-
Very alkaline immobilized pH gradients for two-dimensional electrophoresis of ri - (reply: 1)
-
Gradient Gel Electrophoresis - (reply: 3)
-
SDS-PAGE resolving gel not completely set - (reply: 4)
-
Problem with SDS-PAGE gel - (reply: 13)
-
gel shrinkage ??? - (reply: 2)
-
Wavy Lines in Gel - (reply: 2)
-
Protein extraction for 2D electrophoresis - (reply: 2)
-
Casein gel zymography - (reply: 3)
-
interpreting RNA agarose gel results? - (reply: 1)
-
smear in gel electrophoresis after PCR - (reply: 2)
-
No DNA after gel extraction - (reply: 9)
-
"Wavy" bands in SDS-PAGE gel - (reply: 5)
-
LMW proteins not transfer of gel to membrane - (reply: 1)
-
purify protein from native gel - (reply: 1)
-
Weird gel banding for apoptosis - (reply: 3)
-
Undefined bands in Gel - (reply: 9)
-
Electrophoresis - (reply: 1)
-
Stain in electrophoresis is going from + to - - (reply: 3)
-
agarose gel electrophoresis of RNA - (reply: 1)
-
EtBr in agarose gel and running buffer - (reply: 3)
-
Native gel electrophoresis: Can it tell if a protein is forming dimer? - (reply: 1)
-
trouble in his-tagged protein binding with Ni-NTA agarose - (reply: 3)
-
Agarose gel bands gone (for gel extraction) - (reply: 2)
-
About Gel supershift - (reply: 1)
-
siRNA Agarose gel electrophoresis - Protocol - (reply: 1)
-
Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
-
cDNA test on 1% agarose gel - (reply: 3)
-
Plasmid Extraction from P. Aeruginosa smears in Gel! - (reply: 2)
-
Membrane proteins on 2D gel electrophoresis..... - (reply: 1)
-
Help with bad electrophoresis run. - (reply: 3)
-
Quantify DNA in gel electrophoresis bands - (reply: 7)
-
How to make gradient gel (10-20%), how to control the speed of flow? - (reply: 4)
-
Low yield PCR product after gel purification - (reply: 8)
-
RNA gel electrophoresis for integrity check - denaturing or non-denaturing?? - (reply: 6)
-
Straight bands on DNA gel - (reply: 3)
-
DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
-
Electrophoresis combs - Mini-plus vs Midi - (reply: 7)
-
10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
-
reason of vertical streaks on gels - (reply: 1)
-
Reused glass bottles to make ethidium bromide gels - (reply: 1)
-
Just built a gel chamber, blue LED transilluminator, ect.. selling - (reply: 2)
-
PCR product running on agarose gel - (reply: 32)
-
Purification of RNA binding proteins by avidin agarose beads - (reply: 2)
-
Blue fluorescent color of agarose gel in UV - (reply: 7)
-
gel shift assay by chemilumniscence. - (reply: 1)
-
Electrophoresis problem , please help pals ! - (reply: 8)
-
Western blot question- High % Gel/Low MW Protein - (reply: 7)
-
too much DNA on agarose gel - (reply: 10)
-
polyacrylamide RNA gel - (reply: 4)
-
isoelctric gels hard to stain? - (reply: 2)
-
protein sample floating out of the tricine gel - (reply: 3)
-
Protein Purification from Polyacrylamide Gels - (reply: 3)
-
bands position in agarose gel - (reply: 4)
-
Protein degradation on 2D gels - (reply: 2)
-
SDS PAGE gel storing - (reply: 3)
-
White bands on Cy3 gels - (reply: 1)
-
Annealed oligos vs. non annealed. Electrophoresis pattern. - (reply: 4)
-
mini printer for printing gel images to insert into lab book - (reply: 14)
-
SDS PAGE gels - (reply: 5)
-
Premixing gel solutions - (reply: 2)
-
Odd Bands on Gel - (reply: 3)
-
gel extraction problems with the new kit from Macherey&Nagel - (reply: 5)
-
qPCR: band in gel, but super high Cq? - (reply: 10)
-
How to avoid gel breakage after electrophoresis - (reply: 5)
-
A good UV transilluminator (electrophoresis) - (reply: 4)
-
Agarose gell electrophoresis - (reply: 1)
-
Please look at my gel picture, poor EthBr staining - (reply: 2)
-
Good loading control for native gels? - (reply: 1)
-
Pattern/cloud appearing on agarose gel - (reply: 15)
-
no product on gel after restriction digestion - (reply: 3)
-
Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
-
TAU gel electrophoresis of histones - (reply: 4)
-
Problems with Electrophoresis of RNA - (reply: 3)
-
Problems with having to run the gels for so long - (reply: 14)
-
Ethidium Bromide Agarose Gel Hazard - (reply: 6)
-
Single Plasmid Gel Band - (reply: 1)
-
Buffer in Gel - (reply: 6)
-
how do GST-tags affect gel filtration results? - (reply: 5)
-
problems with recovery of dna from gel and ligation - (reply: 5)
-
Shelf Life of Tricine-SDS-PAGE gel - (reply: 1)
-
Help with PCR and Gel Electrophoresis. Not getting any bands - (reply: 8)
-
QIAquick Gel Extraction - (reply: 2)
-
agarose gel electrophoresis - (reply: 5)
-
Uneven bands denaturing agarose - (reply: 2)
-
Acid urea gel electrophoresis (AUGE) - (reply: 7)
-
storing the loaded gel before running it - (reply: 7)
-
What gel percent and Voltage is needed to separate 2680bp and 2600bp DNA nicely - (reply: 7)
-
Smear on agarose gel from QIAGEN extracted plant samples - (reply: 1)
-
E gels and the E gel opener - (reply: 1)
-
SYBR gold post-stain makes blurry gels - (reply: 1)
-
Dyes for RNA in Urea-polyacrylamide gel - (reply: 1)
-
Coomassie stain forzen and thawed gel? - (reply: 1)
-
PCR band moves lower than its size on agarose gel - (reply: 3)
-
IEF gel detaching during run - (reply: 7)
-
Agarose gel electrophoresis of RNA - (reply: 5)
-
What causes surface fluorescence on agarose gels? - (reply: 6)
-
Problems with PAGE gels - (reply: 1)
-
oligo continually appears less on gel compared to other same load - (reply: 2)
-
can coommassie stained gel be western blot - (reply: 5)
-
carpet in gels for PCR products - (reply: 2)
-
What % native urea gel do I use for Fab and F(ab')2? - (reply: 2)
-
protein extraction from GEL - (reply: 5)
-
After PCR, One Band on 1.2% Agarose but 2 bands on 8M Urea, 8% PAGE - (reply: 1)
-
Odd gel run following PCR - (reply: 28)
-
Problem with visibility on third comb in gels - (reply: 4)
-
how much time for prepared gel to keep bfore loading? - (reply: 5)
-
Transfer of proteins from gel to PVDF for short period - (reply: 3)
-
Product help: Inexpensive Agarose Gel - (reply: 1)
-
what is your DNA concentration after gel extraction? - (reply: 5)
-
staining artifacts in polyacrylamide gel ?? - (reply: 2)
-
Vertical Vs horizontal electrophoresis - (reply: 3)
-
RNase h electrophoresis - (reply: 1)
-
RNA degradation problem, only one band on the gel - (reply: 3)
-
rtPCR working but the elongated product wont gel! - (reply: 1)
-
Using 4-year old precast gels - (reply: 4)
-
Why do supposedly sharp bands sometimes appear unclear in agarose gel? - (reply: 1)
-
Using out-dated equipment to image agarose gel samples-help - (reply: 1)
-
Second round of PCR after gel extraction fails miserably - (reply: 3)
-
Best gel percentage to separate 24 and 36 kDa proteins - (reply: 3)
-
No bands appearing in DNA gel electrophoresis - (reply: 1)
-
What does percentage mean when dealing with stacking and running gel for SDS pag - (reply: 6)
-
What percentage should I use for a stacking gel? - (reply: 1)
-
What percentage gel should I make? - (reply: 1)
-
White big cloud in agar gel electrophoresis - (reply: 13)
-
Signal stuck on top of seperating gel - (reply: 1)
-
SDS-PAGE gel and running buffer - (reply: 3)
-
After I run my pcr on agarose the DNA is still in the well - (reply: 3)
-
Agarose Gel - (reply: 1)
-
Denaturing agent Acrylamide-bisacruylamide gel - (reply: 6)
-
What's the difference between megnatic beads and agarose beads to IP His-tag - (reply: 3)
-
Vertical agarose gel electrophoresis for DNA - (reply: 1)
-
Running Primers on Agarose Gel - (reply: 6)
-
strange bands shape agarose gel - (reply: 2)
-
Estimating size of PCR products in a agarose gel - (reply: 4)
-
Ladders not resolving on agarose gel - (reply: 6)
-
IEF migration to middle of gel, no separation - (reply: 2)
-
how to dissolve Polyacrylamide gel? - (reply: 8)
-
2-D electrophoresis of sperm membrane extracts - (reply: 9)
-
Can we use denatured DNA standards on RNA formamide gels - (reply: 2)
-
Home-made SDS-PAGE gels - acrylamide +/- bis? - (reply: 1)
-
purification of digested vector by agarose eletrophoresis problem - (reply: 4)
-
Running multiple gels/blots at the same time (>5 blots) - (reply: 6)
-
restaining of coommassie destained gel - (reply: 1)
-
agarose gel electrophoresis of plasmid extraction - (reply: 12)
-
how to make nondenaturing gradient gel - (reply: 2)
-
Pulsed Field Gel Electrophoresis - (reply: 4)
-
Sphere staining protocol (agarose) - (reply: 3)
-
How to interpret this agarose gel? - (reply: 6)
-
Ligation gel picture interpretation - (reply: 4)
-
Is there an optimum % agarose or... "more is better"? - (reply: 2)
-
Western gels running super slow! - (reply: 3)
-
How to use the OWL Gel Casting System? - (reply: 2)
-
RNA electrophoresis - (reply: 4)
-
DNA ladder running faster than samples during agarose gel electrophoresis - (reply: 1)
-
Troubles with casting Gradient Gels - (reply: 2)
-
Polyclonal antibody production using SDS-PAGE gel fragment - (reply: 3)
-
total RNA Extraction gel control - (reply: 1)
-
which gel concentration can separate the 7.5 kDa protein? - (reply: 6)
-
PAGE gel recipes - (reply: 1)
-
SB buffer and agarose: Fragments less than 100 bp - (reply: 1)
-
Can proteins with sizes smaller than 60 kda be seen as bands in a 6% gel western - (reply: 3)
-
Protein of 160 kda is present on 6% gel but gapdh housekeeping protein is not. - (reply: 6)
-
PEG 8000 precipitation/ gel electrophoresis - (reply: 5)
-
Software to construct a cladogram from a gel - (reply: 2)
-
Empty agarose gel after electrophoresis - (reply: 1)
-
Bright bands of PCR products stick in gel wells - (reply: 1)
-
DNA Electrophoresis and SB (sodium borate) Buffer - (reply: 3)
-
Low A260/A280 ratio after gel purification using Promega PCR Clean-up System - (reply: 2)
-
polyacrylamide gel problem....slipping - (reply: 5)
-
Anoyone knows a good reliable gel documentation system? - (reply: 4)
-
how to seperate double-digestion products on agarose gel - (reply: 14)
-
Samples leaking out of wells in gel - (reply: 2)
-
Didint get any band during gel electrophoresis even though DNA was there - (reply: 5)
-
syto9 for DNA gel electrophoresis - (reply: 1)
-
I can't see anything in my gel. I need help - (reply: 1)
-
Help with DNA acrylamide gel - leaking and errors during gel running - (reply: 4)
-
Bradford reagent for staining gels? - (reply: 1)
-
wrong size in agarose gel, right size in sequencing - (reply: 7)
-
1D analysis electrophoresis software for automated labelling - (reply: 3)
-
Weird DNA electrophoresis result - (reply: 4)
-
DNA migrate differently in agarose vs PAGE gel - (reply: 4)
-
RNA protein complex interaction in 4-12% NU PAGE gradient gel - RNA PROTEIN bEHAVIOR IN GRADIENT GELS (reply: 1)
-
clear bands on agarose gel but problems in melting curve after qPCR - (reply: 2)
-
poor seal between stacking a seperating gels - (reply: 4)
-
discarding acrylamide gels?? - (reply: 2)
-
Agarose - (reply: 7)
-
IEF technique utilizing Multiphor II Electrophoresis System - I am totally a newbie (reply: 9)
-
Can Current affect to the samples in the edge/periphery of the gel? - (reply: 1)
-
Problem with protein resolving in electrophoresis - (reply: 7)
-
RNA degradation during electrophoresis?? - (reply: 2)
-
gel question - (reply: 3)
-
electrophoresis rflp help - (reply: 1)
-
Stacking gel for DNA PAGE - (reply: 2)
-
Heavy Post-translational modifications - Multiple bands on gel (reply: 2)
-
Bioanalyzer and RNA gel - (reply: 2)
-
EMSA + WB - Transferring 6% native gel (reply: 1)
-
SDS Page heat up and "fry" the gel and protein (Western Blot) - (reply: 2)
-
storing SDS-PAGE gels for W.Blot - (reply: 7)
-
acrylamide gel for low molecular weight protein - (reply: 2)
-
how many samples should i prepare? - about 2 dimensional electrophoresis (reply: 2)
-
The Best Gel Extraction Kits - The Best Gel Extraction Kits (reply: 9)
-
agarose gel has melten - (reply: 4)
-
Dna after gel extraction, single or double stranded? - (reply: 1)
-
Checking RNA on gel after DIG labeling - (reply: 1)
-
Gels 'blobby' - (reply: 2)
-
is it degraded RNA? - I dont get a clear 3-band after electrophoresis (reply: 6)
-
Gel filtration chromatography - for fractionation of lysate (reply: 4)
-
no band observed for bacterial genomic DNA during agarose gel electrophoresis - (reply: 9)
-
Extraction of 12 bp DNA from polyacrylamide gel - (reply: 2)
-
Guanidine thiocynate contamination in gel extraction - Precipitation vs column (reply: 1)
-
SDS-PAGE gel wont separate small proteins - (reply: 5)
-
SDS Acrylamide Gel Equipement - Need to purchase best and cheapest new equipment (reply: 3)
-
PCR Gel nonspecific band - (reply: 13)
-
Single-digested vector shifts downward on gel - (reply: 1)
-
Funny DNA bands on Agarose gels - (reply: 6)
-
leaving gel in buffer ! - (reply: 3)
-
where are they on SDS-PAGE gel of Co-IPed complex??? - (reply: 1)
-
2d gel technique - (reply: 2)
-
Gel Extraction using buffer QG then phenol/chl - Can I? (reply: 1)
-
Tricine-SDS gels blotting problems - (reply: 4)
-
native PAGE gel - (reply: 2)
-
Method to dissolve agarose gel? - (reply: 2)
-
Problem loading DNA / agarose gel - (reply: 14)
-
Which buffer do you use for gel electrophoresis? - (reply: 9)
-
Agarose Gel Disposal - (reply: 13)
-
Problem Viewing bands in Agarose Gel - (reply: 6)
-
Agarose gel loading sample pops out - Sample pops out when loading gel (reply: 3)
-
agarose gel electrophoresis - (reply: 3)
-
plasmid isolation - sth lights up in the well of agarose gel (reply: 2)
-
No bands on agarose gel after restriction digestion. - (reply: 10)
-
Gel filtration Chromatography - (reply: 1)
-
Gel Electrophoresis Smearing Problems - Molecular biology always a mystery! (reply: 4)
-
Stacking Gel Polymerization - (reply: 4)
-
not "that" 2D gel - (reply: 2)
-
Extra band on gel after miniprep - (reply: 6)
-
3 distinct bands of gDNA on gel - (reply: 2)
-
Dry protein gels without cracking - (reply: 5)
-
power supply electrophoresis - (reply: 11)
-
Fat bands on 15% SDS-PAGE gel - (reply: 10)
-
can i use trypsin to collect cell sample for 2d gel analysis? - (reply: 3)
-
resolving gel pourcentage - (reply: 3)
-
Total RNA only produce 1 band in gel - (reply: 2)
-
Gel gets squiggly bands for low molecular weight proteins - (This is driving me insane) (reply: 7)
-
How to modify electrophoresis chamber - (reply: 3)
-
NuPAGE Gel get smaller after staining o/n - (reply: 1)
-
RNA in native gel - (reply: 3)
-
gel documentation system broken - any ideas how to fix? (reply: 2)
-
How to reduce electrophoresis times - Impatient me needs faster results (reply: 16)
-
question about plating cells - gel or no gel? (reply: 2)
-
DNA, Gel - Possible reasons for DNA Gel Smear (reply: 12)
-
TBE/Agarose Gel Concentrations - (reply: 3)
-
Agarose gel to separate btween 4800bp and 4850bp - (reply: 3)
-
Electrophoretic Mobility Shift Assay - no free probes shown in samples (reply: 3)
-
Denaturing gel electrophoresis for qPCR - (reply: 3)
-
uncut plasmid dna vs linearized plasmid gel - (reply: 1)
-
Lithium electrophoresis buffer - Why does it not work? (reply: 2)
-
Run agarose/formaldehyde gel for 5 mins before loading samples? Why? - (reply: 1)
-
sds page - hot hot hot - buffer too hot - gel burnt (reply: 6)
-
PCR, using gel extracted band as a template - (reply: 4)
-
Which gel concentration - (reply: 4)
-
Glyoxylated RNA and SYBR Safe - Running glyoxylated RNA on a SYBR safe agarose gel - NO BANDS (reply: 1)
-
Combining forward and reverse primer gives different size on gel - (reply: 2)
-
Agarose Gel Electrophoreses - What makes it fluoresce? - (reply: 3)
-
TBE Acrylamide/Formamide gels running slowly - (reply: 4)
-
microinjection of zebrafish - the agarose plate - where to buy the mould for the grooves (reply: 1)
-
Blue Native PAGE - Problems with westerns of Blue Native Gels (reply: 3)
-
making gel electrophoresis - (reply: 1)
-
Running a western with cell media in gels? - (reply: 11)
-
Gel electrophoresis - (reply: 4)
-
running problems SDS-PAGE gel (zymography) - (reply: 4)
-
Agarose gel electrophoreses for PCR products ? - (reply: 4)
-
Better images of agarose gel - My bands are not clear when i take a image for processing (reply: 3)
-
Estimation of DNA Quantity on an Agarose Gel - (reply: 5)
-
Gel electrophoresis - Sudden untidy bands despite changing buffer, low yields from gel purif (reply: 4)
-
Electrophoresis: sometimes mA=0 - (reply: 3)
-
low melt agarose - (reply: 1)
-
Agarose Gel electrophoresis - (reply: 2)
-
Problems with qiaquick gel purification kit - cloning (reply: 10)
-
Protein Gel Resolution - Unable to see proteins with a molecular weight less then 14 kDa (reply: 4)
-
strange problem in PAGE gel - (reply: 12)
-
Anomalous asymmetrical gel - (reply: 1)
-
Noisy fluorescent gels - (reply: 1)
-
Anyone know the margin of error for PCR amplicon size visualized on an agarose g - Margin of Error (reply: 2)
-
got smear on the PCR gel !!! - (reply: 4)
-
MaestroGen UltraSlim LED illuminator - for DNA gel (reply: 1)
-
Gel Electrophoresis Question - (reply: 12)
-
Backwards electrophoresis electrodes - Can you dig it? (reply: 1)
-
resolving and stucking gel solutions - (reply: 2)
-
SDS PAGE - Bubble inside the Gel (reply: 5)
-
serum separation: transparent gel prevents serum collection! - (reply: 2)
-
phage DNA - DNA wont migrate on agarose gel (reply: 3)
-
checking sonication using chelex and agarose gel - (reply: 10)
-
Alkaline Lysis Mini prep smeared on gel - (reply: 3)
-
Gel Extracting a 15kb Band? - Quick and Easy method (reply: 3)
-
uncut vector contamination in gel purified band - (reply: 2)
-
Agarose gel electrophresis - (reply: 3)
-
Genomic DNA electrophoresis? - (reply: 1)
-
how to determine gel percentage? - cleaved caspase 3 (reply: 1)
-
polyacrylamide gel storage - (reply: 3)
-
RNA degradation in collagen gels - (reply: 1)
-
making gel in advance - to save time (reply: 7)
-
Agarose gel bands smear/ drag problem - (reply: 4)
-
About Smear on Agarose GEL electrophresis of miniprep purified plasmid - (reply: 7)
-
gel does not polymerize - (reply: 12)
-
Gel Storage for PAGE - Advance casting of polyacrylamide gels (reply: 2)
-
How many volts/milliamps to run gel at? - (reply: 2)
-
EMSA gels - (reply: 2)
-
6.7%Polyacrylamide/50% Urea gel - (reply: 1)
-
Migration Distance on a Gel - (reply: 2)
-
Varied resolution in the same gel - (reply: 2)
-
Large DNA extraction from an agarose gel - (reply: 8)
-
Strange SDS gel after Western Blotting - (reply: 1)
-
Lost protein after gel filtration - Gel Filtration (reply: 6)
-
Electrophoresis problems - (reply: 6)
-
how to do gel documentation - (reply: 2)
-
Irregularities in width of samples running on SDS-PAGE gel - (reply: 3)
-
Does agarose go bad? - (reply: 14)
-
genomic DNA extraction - why there appears a smear on the gel photo (reply: 11)
-
Problem- wierd 2D gel image - (reply: 5)
-
Agarose gel electrophoresis question - (reply: 1)
-
H3 in tris tricine gels - (reply: 2)
-
Microcentrifuge Gel Filtration Question - (reply: 4)
-
SB Buffer and Page Electrophoresis - (reply: 3)
-
What causes this offset band on RIPA gel? - (reply: 4)
-
Running real time pcr product on gel?.. - (reply: 4)
-
DNA stuck in gel well - some samples are stuck in the agarose-gel well and don't migrate (reply: 4)
-
DNA quantification after gel purification - (reply: 6)
-
Please help me to choose right Gel imager!!! - (reply: 2)
-
Denaturing formaldehyde agarose gel for RNA - Once formaldehyde is added, I see no bands (reply: 8)
-
Breast Feeding and Ethiduim Bromide - Can you make agarose gels? (reply: 10)
-
Western blot problems - No or vey little transfer, expanding gel in transfer (reply: 5)
-
PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
-
Problem with gel extraction of DNA. - (reply: 1)
-
GHOST in Electrophoresis - (reply: 18)
-
Akrylamide Gels - (reply: 2)
-
gel filtration.. - (reply: 1)
-
BCA Assay and SDS-PAGE Gels Do Not Agree - (reply: 2)
-
Making gels in advance - (reply: 2)
-
No protein spots in 2D gel - Silver staining! - (reply: 17)
-
agarose gel electrophoresis - fluorescence which will not migrate from well and does not disappear a (reply: 5)
-
Probelm with gel filtration - (reply: 4)
-
3% NuSieve gel electrophoresis - Why 1kb ladder doesn't move well? (reply: 3)
-
Maxiprep using alkaline lysis, very high OD, but low intensity on gel - (reply: 2)
-
electrophoresis/ difference between expected size and result/ what's the alt - How to confirm the band means the expected DNA? Sequencing&what else? (reply: 5)
-
Agarose / SDS Page - (reply: 2)
-
GEL SMEAR - (reply: 4)
-
The end of the stacking gel? - EZ-Run, PRIME gel, NEXT GEL, etc. (reply: 1)
-
Horns like appearance on SDS-PAGE Silver stained Gel - (reply: 7)
-
problem about DNA electrophoresis - the DNAs become two layers in the gel (reply: 4)
-
No SDS in gels - (reply: 12)
-
densidometric analysis with scans - no gels available!!! (reply: 4)
-
SDS-PAGE: Protein bands broaden as it migrates down the gel like a pyramid! - (reply: 2)
-
Smearing on ategarose gel of real time pcr product??? - (reply: 1)
-
Extraction of DNA smaller than 100 bp from Agarose Gel - (reply: 6)
-
agarose gel of ligated DNA doesn't show any bands - (reply: 2)
-
How to view 75 bp DNA in gel - (reply: 4)
-
gel documentation sysytem - (reply: 7)
-
Gel Running Crooked?? Please help.. - (reply: 7)
-
peptide extraction tricine gel - (reply: 1)
-
the ChIP agarose gel results - Should the input always be more than the IP one? (reply: 2)
-
Can I leave DNA in agarose gel piece overnight at 4oC before extraction? - Can I leave DNA in agarose gel piece overnight at 4oC before extractio (reply: 2)
-
DNA damage in gel - (reply: 8)
-
Software for labeling gel picture - (reply: 1)
-
Can i use agarose gel to seperate 80bp - (reply: 2)
-
Bands gradually weaken and disappear after putting the gel on the UV transllumin - (reply: 3)
-
Re-using unloaded agarose gel - (reply: 6)
-
PCR product appear two close band in my gel - (reply: 15)
-
Agarose Gel Electrophoresis Problems - DNA bands appear to be weird on the gel (reply: 3)
-
Destaining a coomassie sttained gel - (reply: 14)
-
5% agarose - (reply: 4)
-
Too much failure in electrophoresis, am I doing the gel correctly? - (reply: 10)
-
Desperate! Loss of basic proteins on 2D gels - (reply: 5)
-
iam not sure from my plasmid isolation result - plasmid gel analysis (reply: 2)
-
Sodium borate buffer gel problems - (reply: 5)
-
help with gel shift assay - (reply: 2)
-
Run PCR amplifications in agarose gels - (reply: 3)
-
Aggregated samples at the top of SDS-PAGE gel - (reply: 3)
-
Tips for visualizing very faint bands in agarose gels? - (The PCR ain't going to get better, so the gel must!) (reply: 15)
-
Silver stained 2-d gel BLOB!!! - (reply: 1)
-
Stacking gel recipe for Acidic Native Gel? - (reply: 1)
-
Problems with small peptide electrophoresis - (reply: 2)
-
EMSA gel problem - (reply: 2)
-
Harvesting cells off of collagen gels - (reply: 1)
-
Polymerase Chain Reaction (PCR) - Anylyzing my PCR gel (reply: 6)
-
Simple gel question - (reply: 7)
-
DNA agarose gel electrophoresis - sum1 can teach me interpret this gel image.. (reply: 3)
-
2 d gel electrophoresis consumable - any brand? (reply: 1)
-
Pulse field gel electrophoresis - (reply: 2)
-
PCR bands on gel electrophoresis - (reply: 5)
-
to keep sds-pa gels - (reply: 2)
-
hey guys,what do you think the Anti-Flag M2 agarose beads is made of? - I want to make some homemade agarose beads,which haven't been crosslin (reply: 3)
-
Hey guys,what do you think the Anti-Flag M2 agarose beads is made of? - Well,actually I want to make some homemade agarose beads,which haven't (reply: 5)
-
Smearing on gel in PCR products - (reply: 5)
-
Protein bands on the bottom of the gel - (reply: 5)
-
Increasing Separation of Bands on SDS PAGE gel - Increasing Separation of Bands on SDS PAGE gel (reply: 2)
-
spot removal from 2D gel and proteomics - help! (reply: 1)
-
Tube gel IEF problems - Polymerization... (reply: 2)
-
Pipette tips for gel electrophoresis? - (reply: 4)
-
Bands in a miRNA gel - (reply: 1)
-
problems with qiagen gel extraction kit - (reply: 7)
-
How to remove urea from PAA gel? - RNA silver staining (reply: 1)
-
Gel Electrophoresis band problems - (reply: 3)
-
Protogel Quick-Cast - Has anybody used this to make their protein gels? (reply: 1)
-
RNA gel to test DIG labeled RNA probe - (reply: 1)
-
wacky RNA gel - (reply: 1)
-
Inseparable insert in gel purification of the vector - (reply: 3)
-
Problem with protein samples - No bands detected at all on gel (reply: 2)
-
NuPAGE bis-tris gel transfer buffer question - (reply: 1)
-
question about bigdye sequencing reaction & capillary electrophoresis - (reply: 3)
-
using cell lysate to run native gel? - (reply: 1)
-
Why do gel electrophoresis buffers need an electrolyte? - (reply: 4)
-
Gel loading dye : sample ratio?? - have to follow strictly? (reply: 6)
-
Gel electrophoresis problem - (reply: 7)
-
dna smear in electrophoresis - (reply: 6)
-
LB and SB electrophoresis media - (reply: 1)
-
Electrophoresis gel boxes and geldoc system - (reply: 2)
-
3T3 cells - Collagen Gel (reply: 1)
-
Storage of gel extracted DNA before ligation - (reply: 3)
-
DENATURING AGAROSE GEL FOR RNA - (reply: 1)
-
Not sure of what % to use for gels - (reply: 6)
-
Protein identification in 2D gel - (reply: 1)
-
why the bands migrate lower in the gel EMSA? - as I increase protein concentration (reply: 1)
-
Making a 3% agarose gel. - (reply: 4)
-
no separation of MW marker on gel - (reply: 1)
-
Expired pre-cast gels - (reply: 4)
-
PCR troubleshooting - two band after agrose electophoresis (reply: 4)
-
Viewing Gels - (reply: 3)
-
how to extract protein from agarose? - (reply: 1)
-
I can't resolve 2 proteins (LC3-I and II) in Tricine gels - (reply: 5)
-
SDS-PAGE gel staining problem - (reply: 1)
-
Gel loading dye recipe - trying to DIY Qiagen gel loading dye (reply: 4)
-
Separating PCR product on agarose gel with similar sizes - (reply: 5)
-
Interpreting a gel - (reply: 3)
-
Interpreting a Plasmid Profile gel photo - (reply: 3)
-
Agarose Gel Electrophoresis - How to prove two bands are same size? - (reply: 3)
-
Protein size and gel percentage - (reply: 7)
-
My acrylimide gel rips. - I need help keeping it frmo ripping all the time (reply: 3)
-
Does purifying PCR probes for EMSA from EtBr gel interfere with binding? - (reply: 1)
-
RNA gel electrohoresis - (reply: 1)
-
DNA supercoiling on agarose gel -> reference needed - (reply: 1)
-
DNA Ladder Issues - DNA Ladder Agarose Gel (reply: 5)
-
SYBR melting curve vs RT-PCR gel - (reply: 1)
-
why gel is not running in straight line?? - (reply: 13)
-
EXtraction of DNA bands from acrylamide gel - (reply: 4)
-
Problems in runnging Native gel - Problems in runnging Native gel (reply: 4)
-
qPCR - no signal detected but plenty of product on gel - (reply: 3)
-
Heat inactivation/Gel purification or both? - Normally I purify from gel. Should I inactivate the enzymes? (reply: 2)
-
Another band after plasmid purificaiton - ran the plasmid DNA on a gel (reply: 2)
-
casting agarose gel troubleshooting - smeared bands (reply: 8)
-
densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)
-
How to analyze the ingredients of protein bands in native-PAGE gel? - help (reply: 3)
-
Pulse Field Gel Electrophoresis - smear at lower molecular weight bands (reply: 6)
-
the bromophenol blue band is skew during agarose electrophoresis - (reply: 1)
-
RNA not visible on gel :( - (reply: 3)
-
how better is 7Cm 2D gel electrophoresis for separation - is anyone doing that? (reply: 1)
-
Using DNA in gel extract kept at -20ºC - (reply: 2)
-
Help! Plasmid disappearing after mini-prep. - A260 shows good quality DNA, but nothing appears on gel. (reply: 3)
-
no front in sds-electrophoresis - no front is formed on my gel - why? (reply: 1)
-
UV-transparent gel tray - Gel electrophoresis (reply: 3)
-
Get rid of particles on gel surface after SYPRO-Ruby staining - (reply: 1)
-
Fast protocol for RNA agarose gel electrophoresis - (reply: 1)
-
What gel thickness and numebr of combs do you use? - and why?? (reply: 1)
-
Uneven leveling in resolving gel - SDS-PAGE resolving gel woes (reply: 6)
-
Multiplex PCR - Multiplex PCR and gel electrophoresis (reply: 1)
-
want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
-
Gel electrophoresis and purifying dna - rnase treatment or not? (reply: 6)
-
Blue Native Gel - denaturing proteins after transfer? - (reply: 9)
-
[vote] The best Gel extraction kit - which brand do you think is the best ? (reply: 11)
-
Blue Juice loading dye & Gel extraction kit - HELP :( (reply: 6)
-
denature gel shrink, help - (reply: 5)
-
Agarose gel with EtBr exposed to light over night. - (reply: 3)
-
help-how to extract fluo-labeled DNA from agarose gel - (reply: 1)
-
strange agarose gel - (reply: 9)
-
cloudy gel - (reply: 2)
-
How much RNA is needed to see rRNA on a gel? - (reply: 3)
-
Gel shift (emsa) with 35S label? - (reply: 5)
-
A260/230 readings post gel extraction - (reply: 19)
-
pancreas RNA gel run consult - (reply: 1)
-
Agarose: DNA/dye mixture doesn't stay in the gel? - Thanks for any and all help received! =] (reply: 12)
-
cracking gel problem - (reply: 1)
-
bubbles between gel and glass plate - (reply: 9)
-
How to extract protein from SDS-PAGE gel? - (reply: 7)
-
i touch agrose gel which contain ethidium bromide - (reply: 6)
-
Most strange sds page gel ever!!!!! - (reply: 5)
-
Gel Purification problem..Help~! - (reply: 7)
-
migration rates of loading dye in sodium borate gel - (reply: 1)
-
how to solve fungus problem on SDS PAGE gel - (reply: 4)
-
PCR product stays on agarose gel well !! - I need help, what is it happening? (reply: 11)
-
dna not running for electrophoresis - (reply: 9)
-
sonication in ChIP / nonReverse - nonReverse gel run check ok or not (reply: 1)
-
bad bad Agarose gel - (reply: 9)
-
uneven on gel electroforesis - uneven on gel electroforesis (reply: 4)
-
protein degardation during gel filteration - (reply: 2)
-
abnormal band in agarose gel - Urgent help required (reply: 3)
-
Running Polyacrylamide gel in acidic conditions - Adicid denaturing PAGE (with or without SD?) (reply: 1)
-
proteins stuck at interface between stack and separating gel - (reply: 2)
-
smear on gel after tca precipitation - (reply: 3)
-
Fragmented DNA doesn't appear as bands on agarose gel - (reply: 5)
-
Gel extraction help.... desperate! - (reply: 6)
-
Problem with DNA extraction from 1% agarose Gel - No DNA using Qiagen Qiaek 2 and Spin column kits (reply: 9)
-
problem with SDS-PAGE - Sample stuck in the wells of the gel (reply: 5)
-
volume of DNA required (PCr product ) in agarose gel - (reply: 11)
-
separation of dna fragments in agarose gel electrophoresis - (reply: 7)
-
SDS gel - (reply: 2)
-
problems with DNase1 footprinting - DNA won't enter a denaturing urea polyacrylamide gel (reply: 2)
-
Gel shift with large DNA fragments (>1 kb) - Can I still do EMSA? (reply: 1)
-
prearing gel in advance - (reply: 6)
-
need help with agarose gel electrophoresis and restriction enzyme - (reply: 7)
-
PCR and gel purification problem - did I screw this up (reply: 4)
-
DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
-
no band in gel - gel electrophoresis (reply: 1)
-
Agarose gel electrophoresis troubleshooting - (reply: 37)
-
Gel Analysis Software - which to use (reply: 3)
-
elution of dna from gel - (reply: 5)
-
Agarose gel problem.. plz help - (reply: 11)
-
Question about DNA electrophoresis - (reply: 1)
-
Agarose gel electrophoresis problems - (reply: 7)
-
PFGE gel - Can I reuse pulsed field certified gel for PFGE? (reply: 2)
-
no 18S band on RNA agarose gel - (reply: 1)
-
Reamplification after agarose gel extraction - problems with reamplification of a band after purification form gel (reply: 4)
-
Freedom Rocker - Save yourself from babysitting your gels! - Save yourself from babysitting your gels! (reply: 2)
-
gel extraction protocol for small amount of starting material - (reply: 3)
-
types of gel stains - (reply: 7)
-
5' RACE products appearance on 1.5% agarose gel - (reply: 8)
-
Gel mix, acrylamide/bis, Ammonium persulfate, etc disposal - (reply: 1)
-
Running gel after RT-PCR - (reply: 5)
-
Heating RNA samples for electrophoresis - (reply: 3)
-
Importance of Reducing Agent - Apparent protein size on gel (reply: 2)
-
small PCR products on agarose gel? - (reply: 8)
-
Gel Electrophoresis Interpretation - (reply: 3)
-
protein size gradient gel - (reply: 10)
-
Tris Glycine gel running settings - (reply: 4)
-
Problem with "smiling effect" on SDS-PAGE gel - (reply: 11)
-
Cast Bis Tris Gel with fibers in the wells - (reply: 7)
-
a doubt about PCR gel purification - (reply: 4)
-
Best lysis buffer for IEF/ 2D gel? - (reply: 2)
-
funny patterns on a gel - (reply: 4)
-
low melting point agarose - (reply: 9)
-
denaturing proteins in gel - (reply: 1)
-
Problem with DNA purification from agarose gel - (reply: 3)
-
Agarose gel cutting - (reply: 8)
-
Any good software for 2D gel analysis - (reply: 1)
-
No band in the gel after ligation - (reply: 6)
-
RNA appearance on 1.5% gel after DNase I digestion - (reply: 2)
-
miRNA Northern Blot gel - (reply: 4)
-
Rcovery of proteins from gels - (reply: 2)
-
Too cold for agarose beads to survive? - Antibody Affinity Purification (reply: 5)
-
Problem with the transformation - I could find the insert in plasmid when I did Gel electrophoresis (reply: 1)
-
Proteins not running till the end of the gel - (reply: 2)
-
How does the protein fixation on SDS gel work? - (reply: 3)
-
PCR-based Site-directed mutagenesis - clear bend on the gel but no colony on the plate (reply: 7)
-
Problem with Colony PCR - Problem with gel for colony PCR (reply: 3)
-
Gel purification of vector DNA - low yield - (reply: 26)
-
gel purification or ligation problem - (reply: 3)
-
gel shift in western blot - (reply: 3)
-
Problem with running SDS page gel - (reply: 6)
-
RNA electrophoresis - (reply: 3)
-
electrophoresis problem - (reply: 1)
-
Which Electrophoresis Gel documentation system you like - (reply: 5)
-
agarose gels - (reply: 1)
-
Different dyes for gel electrophoresis - (reply: 9)
-
Is immunoblotting possible after the gel has been silver stained - (reply: 2)
-
protein disapeared from gel! - (reply: 11)
-
Loading samples into gel: How much was it? - (reply: 9)
-
strange double band in electrophoretic pattern - (reply: 2)
-
2d gel analysis software??? - (reply: 6)
-
Gel Electrophoresis.... - ....some samples running the wrong direction (reply: 5)