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Cloning Related Discussions
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TA cloning for sars cov 2 nucleocapsid protein. - (reply: 4)
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Cloning a zebrafish gene? - (reply: 1)
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Designing Crispr guides and cloning into appropriate vectors - (reply: 1)
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Cloning protein of interest into lentiviral vector - help - I have no experience - (reply: 20)
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Trouble with PCR after cloning - (reply: 1)
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Troubleshooting with restriction enzymes during cloning - (reply: 5)
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Favorite polymerase for general cloning? - (reply: 3)
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restriction enzyme pcr cloning - (reply: 1)
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polb cloned in pET not expressed - (reply: 2)
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Restriction Digest with pGEMT for TA cloning - (reply: 19)
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Cloning of big inserts - (reply: 2)
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TA cloning..Urgent Help!! - (reply: 4)
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Wrong or No fragment in Directional Cloning Vector - (reply: 8)
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Subcloning issue - (reply: 2)
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pGEM T Easy subcloning - (reply: 1)
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Target gene cloning - (reply: 4)
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Cloning issue with bacteria growing in primary media but not in secondary - (reply: 3)
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Problem with possible leaky expression in cloning - (reply: 4)
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Luciferase assay cloning - (reply: 2)
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Problem with restriction digestion of cloned PCR product - (reply: 5)
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SLIC Cloning Failure - (reply: 2)
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Cloning - Miniprep plasmid contaminated by gDNA? - (reply: 1)
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Polymerase mixture for blunt ended fragments - (reply: 2)
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Cloned gene in pET system not being expressed - (reply: 10)
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Problem with subcloning genescript synthesized genes - (reply: 2)
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Loss fluorescence when clone a fluorescent sensor into a differenet vector - (reply: 1)
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Problem with double digest cloning - (reply: 3)
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How good is your cloning efficiency? - (reply: 2)
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Cloning several metabolic enzymes to screen for inhibition, how long? - (reply: 1)
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Cloning a 17 kb contruct - (reply: 2)
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Cloning of an unknown gene - (reply: 4)
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Cloning of an unknown gene - (reply: 3)
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cloning problems using in-fusion kit - (reply: 5)
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Cloning - Ligation problem - (reply: 3)
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How to clone a 2A peptide between two ORFs? - (reply: 2)
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TA Cloning - (reply: 11)
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Troubleshooting with cloning - (reply: 2)
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Suitable ratio of vector and insert in cloning - (reply: 5)
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Cloning a big insert in a small vector - (reply: 2)
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Cloning purified PCR product into cells - (reply: 5)
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Gateway cloning not working. - (reply: 7)
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Insert direction in TA cloning - (reply: 2)
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Problem with stable clone - (reply: 2)
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Should I proceed with clone selection? - (reply: 2)
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Can I loose the protein of interest from a stable clone? - (reply: 2)
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Cloning Woes: Dealing with Viral ITRs - (reply: 1)
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Difficult cloning/ligation/transformation blunt and sticky - (reply: 1)
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Sticky and blunt end ligation in single reaction? - (reply: 2)
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How can we expand molecular cloning further? - (reply: 3)
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Molecular cloning using an ezh2 insert - (reply: 2)
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Subtle change in insert size after cloning - (reply: 6)
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lactic acid bacteria - cloning - (reply: 2)
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Can't isolate plasmid from cloned E.coli - (reply: 4)
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cloning issues - (reply: 3)
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Is important the direction of IRES-GFP in cloning? - (reply: 1)
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Cloning inserts: forward and reverse orientation - (reply: 4)
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What are the error-rates of the TA cloning Kits? - (reply: 8)
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What sorts of cloning strategies are available for creating inducible knockouts - (reply: 2)
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Can mutations be created during cloning? - (reply: 7)
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What is the full name for TA cloning - (reply: 4)
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cloning problem - (reply: 2)
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Cloning of 9mer nucleotide (3 amino acid) sequence! - (reply: 3)
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Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
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Cloning GFP into pCMV-Tag2B vector - (reply: 1)
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Cloning an antimicrobial peptide that may kill the host - (reply: 1)
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primers for cloning - (reply: 1)
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Cloning contamination - (reply: 3)
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How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
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Problem shrna cloning, bad sequencing results - (reply: 1)
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Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
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pcDNA 3.1 Directional TOPO clone issues - (reply: 4)
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Blunt end cloning - (reply: 12)
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Molecular cloning - (reply: 5)
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Nuclease free water for cloning - (reply: 8)
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Directional cloning 2bps overhang - (reply: 3)
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filling the digested vector and insert for blunt end cloning - (reply: 2)
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OVA cDNA clone? - (reply: 2)
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HELP!! Problem with cloning (self-ligated vector) (trying since 4 months - (reply: 4)
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Purpose of PolyA in cloning - (reply: 1)
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Cloning into pVIVO2 vector - (reply: 8)
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Cloning worked, but the expression vector is weird - (reply: 6)
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How to clone a gene whose genome has not been sequenced yet? - (reply: 4)
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Ubiquitin promoter cloning - (reply: 1)
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Cloning two genes in the same plasmid with one promotor - (reply: 4)
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Is this counter-productive cloning or not? - (reply: 8)
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Failed cloning for 1month - TOPO - (reply: 7)
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A modified method for blunt end DNA cloning!!!!!!! - (reply: 2)
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Which method for DNA purification b4 cloning - (reply: 4)
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Designing cloning primes (restriction site and kozak sequence) - (reply: 1)
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Failed TOPO cloning - what happened? - (reply: 9)
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TOPO cloning after Phusion-PCR - (reply: 1)
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Gene cloning with unknown sequence - (reply: 6)
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Failed cloning - different readings - (reply: 10)
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Ideas wanted-How to clone 600bp gene with two 5' 55base overhangs? - (reply: 1)
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Cloning pDsRed-Monomer-N1 - (reply: 1)
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Annexin V clone - (reply: 2)
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Please comment on my Cloning Plan - (reply: 1)
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Cloning problem still not solved - (reply: 2)
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Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
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cloning pEGFP-N1 - (reply: 1)
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Cloning beginner and clueless! - (reply: 1)
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Cloning Problems - (reply: 2)
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Different ways to ensure the successful cloning? - (reply: 2)
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pcDNA 3.1 Directional TOPO clone issues - (reply: 18)
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Sticky and blunt ligation issue - (reply: 6)
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cloning - (reply: 7)
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Help~ Incorrect constructs in cloning - (reply: 3)
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Blunt end is ligating to sticky end? - (reply: 2)
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Failure to BP Clone. Troubleshooting advice? Switch to TOPO TA cloning? - (reply: 2)
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Cannot clone 2kb insert into pENTR D TOPO cloning vector - (reply: 9)
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Sticky to Blunt end (Hypothetical) - (reply: 2)
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Do genes always need to be cloned in the "right direction"? - (reply: 3)
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No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
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Cloning and expression problem - (reply: 5)
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Clone selection for scrambled shRNA transfected cells ? - (reply: 2)
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Design PCR primers for cloning 3 copies of same insert - (reply: 2)
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cloning help; high salt level in vector - (reply: 4)
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problem with gateway cloning: missing part of insert - (reply: 3)
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Type of polymerases compatible for TA cloning - (reply: 7)
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Molecular Cloning Sambrook - (reply: 1)
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golden gate cloning - (reply: 3)
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types of dna cloning methods - (reply: 2)
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excision of fragment during cloning - (reply: 4)
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Unusual cloning issue... anyone else?? - (reply: 4)
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Sequencing of the cloned plasmid - (reply: 5)
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Cloning synthesised genes - (reply: 1)
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4 way cloning into TA Vector - (reply: 10)
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Neeed Help , cloning Confirmation ?! - (reply: 5)
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Puzzling Cloning Problem With a Specific Vector - (reply: 1)
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Newbie to smaI blunt end cloning! - (reply: 1)
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What part of the promoter to clone - (reply: 2)
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gene cloning, where to start? - (reply: 1)
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TA Cloning of ds cDNA - (reply: 6)
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Very difficult cloning project - how to clone something that is not compatible w - (reply: 4)
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Cloning cDNA construct into E.Coli. - (reply: 4)
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Cloning large transgenes - (reply: 2)
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In-fusion HD cloning - (reply: 5)
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Few colonies frm sticky end cloning - (reply: 3)
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Problem with cloning - (reply: 7)
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Too many negative clones using Directional TOPO Cloning - (reply: 5)
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Designing cloning primers for DNMT - (reply: 2)
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Cloning advice - (reply: 3)
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classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
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Negative control antibodies (Anti-mouse C5 Clone BB5.1) - (reply: 1)
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Cloning large fragments - (reply: 9)
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Blunt cloning - No insert, despite lots of colonies - (reply: 3)
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Cloning Mitochondrial Targeting Sequence - (reply: 4)
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problem in cloning - (reply: 2)
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TA cloning: ligation problem or toxic construct? - (reply: 4)
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Designing PCR Primers for cloning - (reply: 17)
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Including ATG in cloning? - (reply: 1)
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gene cloning - (reply: 1)
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Cloning repetitive sequences in mammalian cells - (reply: 3)
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Truncated gene Cloning - (reply: 1)
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Mutation in cloning - (reply: 6)
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Understand the basics of molecular cloning - (reply: 6)
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TA Cloning of alpha-1,3 glucanase: No blue colonies - (reply: 5)
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what is the ORFeome clone? - (reply: 2)
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rescue cloning of a transposon mutant - (reply: 2)
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problem in cloning PCR primer design with restriction site - (reply: 4)
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Cloning at 6 kb gene - (reply: 3)
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Vector insert ratio in cloning - (reply: 3)
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polymerase to use for cloning - (reply: 4)
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Oligo insert cloning - (reply: 3)
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Poor expression in my cloned vector (pMSCV-PIG) - (reply: 1)
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ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
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Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
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Single RE site cloning - (reply: 3)
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Bisulfite PCR and cloning - (reply: 5)
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Cloning not working, all steps checked, clueless - (reply: 1)
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Cloning problem, sticky - blunt ends ligation fail . - (reply: 6)
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Cloned but not expressing! - (reply: 8)
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Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
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Problem with ligation using pJET and pGEM for clone library - (reply: 11)
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I have a 96 tandem array repeat clone but need half of them in new clone - (reply: 4)
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TOPO TA CLONING - LIGATION STEP - (reply: 9)
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pEGFP C-1/N-1 Cloning - (reply: 11)
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Low yield of putative clone compared to empty vector in DH5alpha using pGEMTeasy - (reply: 3)
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Problem with cloning - what is wrong? - (reply: 6)
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cloning: band at different site than desired after RE digestion - (reply: 11)
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No activity of expressed protein obtained from pET32a cloned gene - (reply: 6)
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cloning pcr insert into plasmid - (reply: 2)
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TOPO TA CLONING - MOLAR RATIO - (reply: 2)
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molecular cloning - (reply: 7)
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Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
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Another cloning hell....no transformants - (reply: 1)
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TOPO TA Cloning Troubleshooting - (reply: 1)
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TOPO TA Cloning - (reply: 1)
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No insert in subcloning - (reply: 3)
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Unable to clone in E. coli cells - (reply: 3)
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cloning into pPICZalphaA fails - (reply: 4)
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molecular cloning failure - (reply: 2)
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cloning antisense promoter in pGL3Basic - (reply: 2)
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Help - Troubleshooting cloning into pET28 - all screens positive, but sequencing - (reply: 2)
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Explanation of "In-Frame" cloning? - (reply: 6)
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Cloning Problem! I need help - (reply: 3)
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Help designing primers for use in In-fushion cloning - (reply: 4)
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Repeat of desired mutation in cloning product - (reply: 1)
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cloning a gene in to a plasmid - (reply: 5)
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recognition sites and cloning? - (reply: 2)
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question about PCR and cloning - (reply: 1)
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cloning from cDNA but got a much shorter PCR product - (reply: 4)
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Cloning & Expression Problem in HEK-293Ts - (reply: 6)
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Making a qPCR standard amplicon from cloning, why? - (reply: 4)
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help ,blunt end ligation - (reply: 4)
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can NOT subclone from pgemT-EZ into pmir-Report *help?* - (reply: 4)
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Subcloning - RE digest and ligation and transformation - (reply: 5)
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Making DNA Linkers for cloning purposes - (reply: 1)
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cloning short genomic DNA fragments - (reply: 1)
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How to clone insert with one sticky and one blunt end into vector? - (reply: 1)
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cloning to pET28a - (reply: 1)
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HMK-Tagged PCR Cloning Problem... - (reply: 4)
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CDS CLONING QUERY - (reply: 5)
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unable to amplify an infectious retrovirus clone - (reply: 4)
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Is the Kozak sequence really needed for cloning to express the gene? - (reply: 3)
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Cloning partial CDS - (reply: 5)
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Cloning problem into pGEMT-easy - (reply: 2)
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Cloning problem! - (reply: 8)
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Plasmid clone in BSP - (reply: 1)
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molecular cloning - (reply: 3)
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cloning without phosphorylation - (reply: 7)
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Cloning genetic sequences of proteins. - (reply: 1)
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should I do blunt end my pcr product before ligation - (reply: 2)
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Help with CGL gene cloning problems - (reply: 1)
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PUAST sub - cloning problem - (reply: 3)
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Cloning Problem - Ligation or Transformation - (reply: 4)
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Bisulfite cloning... reading material - (reply: 1)
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cloning into Pgem t easy - (reply: 1)
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I can´t obtain the amount of DNA required for ligation of blunt ends - (reply: 2)
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cloning of a long gene using cDNA - (reply: 1)
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Isolation of a Gene of Interest for Subcloning - (reply: 8)
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Cloning into inducible vector but no expression - (reply: 1)
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weird problem in cloning!! - (reply: 4)
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Cloning HELP- Possible Primer issues - (reply: 6)
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Strain requirements for routine M13 phagemid cloning? - (reply: 1)
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Cloning in pETDuet-1 - (reply: 3)
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PCR cloning...help! - (reply: 5)
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Problems with blunt end ligation - (reply: 3)
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cloning problem - (reply: 2)
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problem in cloning involving partial digestion - (reply: 1)
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Design primers for expression cloning - (reply: 11)
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which software to draw the scheme of cloning strategy - (reply: 5)
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Blunt end ligation insert:vector ratio - (reply: 1)
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Has anyone ever had a large insert when subcloning? Mine is 4.3kb? - (reply: 2)
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Impact of primer sequence on TA cloning - (reply: 3)
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cloning an AT rich gene - (reply: 3)
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Cloning two genes in the same plasmid - (reply: 4)
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Problem: Cloning DNA shuffled band - (reply: 2)
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Transformation /Cloning Problem - (reply: 3)
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a robust multi-gene cloning method - (reply: 2)
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strange subcloning problem - (reply: 9)
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Cloned sequence is not predicted size by western blot - (reply: 1)
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good tutorials/protocols/books on gene cloning - (reply: 2)
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Cloning problem using pET32a vector - (reply: 3)
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Fixing SNPs after cloning - (reply: 5)
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emty vector cloning - (reply: 3)
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Double digest after cloning doesn't give gene - (reply: 6)
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Blunt end PCR product ligation - (reply: 1)
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strange cloning problem - (reply: 9)
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need help with subcloning !! - (reply: 2)
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How to do cloning with NcoI RE in pACYCDuet vector - (reply: 1)
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cloning with nested pcr - (reply: 3)
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cloning blues! - (reply: 12)
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Gateway cloning vector compatibility - (reply: 2)
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Cloning of IL8-promoter - (reply: 3)
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Cloning promoter (overlap with another gene!) - (reply: 3)
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Cloning a promoter - (reply: 2)
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Cloning two fragments into a vector - (reply: 3)
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How do I insert a DNA sequence into a multiple cloning site and add restriction - (reply: 1)
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Problem with Blunt end ligation - (reply: 4)
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Cloning from a pGEMT Easy vector - (reply: 3)
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cloning 2 PCR products into pGEM-T easy vector - (reply: 1)
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cloning problem - (reply: 9)
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Cloning genes into plant vector - (reply: 1)
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Cloning from RNA template: Second strand cDNA synthesis - (reply: 4)
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Blunt ending (T4) + BAP + Ligation in the same buffer?? - (reply: 2)
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Cloning App Released from Life Technologies - (reply: 2)
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gateway cloning: is BP-cloning "reversible" by LR-reaction? - (reply: 2)
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RT-PCR primer design for full length cDNA cloning - (reply: 6)
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Blunt end cloning of 4Kb product - (reply: 3)
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competentce cell, cloning - (reply: 2)
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sequential cloning of multiple PCR products - how to do? (reply: 3)
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Cloning large gene - (reply: 3)
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Glycosilated peptide's cDNA cloning - (reply: 3)
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pWM91 cloning problem - (reply: 4)
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Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
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which is the best plasmid vector for cloning BSP products? - (reply: 2)
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Is a 3.5 kb fragemnt too big to be cloned ? - (reply: 8)
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Cloning into lentiviral vector - (reply: 11)
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cloning failure help plzzz - cloning (reply: 1)
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expreesing cloned vector - having problems with texpreesing cloned gene in vector. (reply: 6)
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PCR cloning problem - could not get colony (reply: 4)
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Cloning Difficulty - Problems with digestion/ligation (reply: 3)
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Lactic Acid Bacteria - Challenges in working with LAB culturing, cloning and plasmid (reply: 7)
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Difference between PCR/cloning DNA in plasmids - (reply: 2)
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Cloning and expressing a mystery 71KDa protein - (reply: 9)
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Insert magically shortening??? - Cloning issues (reply: 2)
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Subcloning Problem - (reply: 3)
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Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
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cloning problem... - (reply: 5)
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cDNA clone - (reply: 1)
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miRNA cloning - (reply: 2)
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PCR: Cloning Primers - (reply: 8)
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Problem cloning wild type E. coli gene... Possible recombination event in DH5alp - (reply: 4)
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Cloning does not work - (reply: 4)
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Help needed with subcloning problem - (reply: 6)
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Mouse huntingtin clone - (reply: 1)
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BLUNT CLONING With EcoNI - (reply: 1)
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Designing a multiple cloning site - (reply: 5)
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blunt and sticky end clonning temperature - (reply: 2)
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Most common mistakes - What mistakes are made during cloning? (reply: 7)
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Cloning direction / orientation? - (reply: 2)
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Is it possible to blunt only either the 5' or 3' end? - (reply: 1)
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optimal size for 3'UTR cloning - miRNA and 3'UTR cloning (reply: 2)
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problem with cloning p16ink4a into pEGFP-C2 - (reply: 1)
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Cloning of ITS region - (reply: 6)
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SmaI blunt end ligation problem... - (reply: 3)
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Cell dilution cloning - Cells cloning (reply: 2)
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Why shouldn't you clone using expression bacteria - (reply: 6)
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metylated cloning vector - restriction digest of the insert (reply: 2)
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Secretion cloning vectors - (reply: 1)
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Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
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HELP! cloning problem with pET22b using NdeI and XhoI sites - (reply: 4)
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TOPO TA Cloning of WGA products? - Is this possible? (reply: 2)
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cloning of race products - (reply: 1)
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Advice on amount of starting template for subcloning - (reply: 3)
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Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
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cloning a 34kb insert - please suggest good vectors (reply: 1)
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Cloning issue - (reply: 3)
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Comment of this cloning strategy - (reply: 3)
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Cloning Problem: HSP90AA1 - Cloning Problem: HSP90AA1 (reply: 1)
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cloning a large insert - (reply: 5)
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Transposase contamination in cloning - (reply: 7)
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Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
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Cloning of a gene containing 31 exon - (reply: 1)
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cloning help - (reply: 3)
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Blunt end ligation problems - Could the ligation of sticky adaptors help? (reply: 2)
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weird bands after minipreps - molecular cloning (reply: 2)
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purification of PCR product for cloning in a vector - (reply: 3)
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Cloning a foreign gene into E-coli - (reply: 1)
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subcloning into bacterial expression vector - (reply: 1)
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cloning into F1 ori of expression vector??? - (reply: 1)
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Where to buy pBluescript-H1 plasmid? - Is it commercially available or do I need to clone it? (reply: 2)
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Digestion of clinical plasmids for posterior cloning - Digestion of clinical plasmids for posterior cloning (reply: 2)
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Cloning a Promoter - (reply: 3)
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Amplification of 3.4 kb product from RNA for cloning - (reply: 5)
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glycerol stock preparation of recombinant clone - (reply: 2)
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Cloning - (reply: 1)
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Anyone make their own PCR cloning vector? - (reply: 5)
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Antibody gene cloning - (reply: 3)
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Which cloning kit should I use - (reply: 2)
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Topo cloning or pGEM-T easy ? - (reply: 3)
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Cloning the coding region of a protein - (reply: 4)
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Unsuccessful cloning with one sticky end & one blunt end - Low transformation efficiency & colonies do not contain insert (reply: 8)
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ta cloning problem - (reply: 2)
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Help: Cloning a 60KB fragment - (reply: 3)
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Cloning Problems/ Help - (reply: 2)
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clone GENE RT-PCT PRIMERS - cloning genes (reply: 1)
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Cloning strategy for a membrane protein - did I do anything wrongly? (reply: 2)
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Molecular Cloning od Recombinant DNA - (reply: 3)
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A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
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obtain a full length cDNA clone WITH RT-PCR - Cloning (reply: 2)
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Dna cloning using NdeI/BamHI using pET11 vector - (reply: 4)
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TOPO cloning problem - (reply: 4)
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Cloning long fragment...got lots of colonies with vectors that have no inserts.. - (reply: 9)
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How to delete a domain in a gene? - cloned plasmid (reply: 12)
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Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
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EcoRI cloning giving only inverted inserts!!?? - (reply: 3)
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cloning more than 1 gene into vector if no IRES available - Xenopus IRES? if not, what to do? (reply: 2)
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cloning very long fragments into vectors using T/A cloning method, - (reply: 2)
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Basics of cloning - HELP! - I'm new to this and need some help! (reply: 7)
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Cloning into a pGEX lacking of one RE consensus - cloning (reply: 1)
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Cloning before sequencing- is it necessary??? - (reply: 14)
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Ligation problem: clones survive selection but nothing in there? - ligation, cloning (reply: 24)
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colony PCR for subcloning gene - (reply: 1)
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Where can I get vector suitable for Ligation Indepdependent Cloning - (reply: 1)
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cloning of a 20 kb insert - (reply: 2)
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cloning of an operon - (reply: 2)
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Cloning problem - (reply: 2)
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Need advice on cloning very small insert into a vector - (reply: 3)
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A cloning nightmare - (reply: 8)
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Cloning problems i.e. Ligation - (reply: 2)
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plasmid incompatibilities with certain sequences - trying to clone a repitious sequence into a pUC based vector (reply: 1)
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cloning problem - (reply: 5)
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How to clone DNA (already cloned in a vector) in to another vector - please help (reply: 20)
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Blunt end filling - Blunt end filling (reply: 1)
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No luck with blunt and sticky ligations, need hlep - Prepare for a thorough read (reply: 10)
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TOPO Cloning - (reply: 5)
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blunt ligation - (reply: 4)
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DNA Sequencing / Cloning ? - (reply: 5)
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fermentas clonejet pcr cloning kit - want opinion (reply: 1)
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Cloning <100 bp fragments into a vector - (reply: 7)
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Cloning <100 bp fragments into a plasmid - (reply: 7)
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HpaI blunt end cloning - (reply: 1)
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New to cloning -- strangely "mature" colonies after ON growth. Any idea - (reply: 1)
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Problem in expression of protein cloned in pcDNA3 - (reply: 1)
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Cloning in pCR2.1 plasmid - (reply: 7)
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Cloning a 3.5Kb fragment for sequencing - P-gem T-easy. (reply: 2)
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Advice on Cloning Strategy - (reply: 4)
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Need help with 7-way ligation. - Help make 7-way reliable cloning strategy! (reply: 1)
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Is it possible to ligate blunt and sticky ends at the same time? - newbie mistake... Please help!! (reply: 11)
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Ligation of Blunt PCR Product - (reply: 1)
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Cloning Help!! - It is getting very annoying... (reply: 13)
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sequence errors created during cloning - (reply: 6)
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PCR & Cloning - (reply: 6)
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Help cloning large insert into large vector - (reply: 7)
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blunt end ligation problem - Self ligation and truncate (reply: 1)
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PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
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Cloning: Randomly recombined Plasmids? - (reply: 6)
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AvrII??? Friend or Foe? - molecular cloning; AvrII; digestion; resistance (reply: 1)
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Cloning problems - (reply: 7)
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promoter cloning PCR problem - what was wrong for my PCR set-up (reply: 3)
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cloned plasmid expression less than original plasmid - after transfection (reply: 4)
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Blunt end ligation - Cant get ligation to work (reply: 4)
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blunt ligation - (reply: 1)
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please help!!cloning problems.. - (reply: 4)
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Cloning - PCR and Cloning problem (reply: 3)
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problem with cloning of dsred plz help - vector cut seems fine, still religation oocurs (reply: 13)
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cloning of GroEL - (reply: 3)
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Problems with High Background with Gateway Cloning - (reply: 1)
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blunt end ligation - how to solve the blunt end ligation?? (reply: 5)
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Removal of 5´ overhangs to form blunt ends - (reply: 1)
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problem with pcr cloning from mouse cDNA - (reply: 2)
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QUESTION about cloning - (reply: 5)
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molecular cloning troubleshooting - bacterial sequences instead of my protein ! (reply: 3)
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strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
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problems in cloning, need help - problems with plasmid stability in bacteria (reply: 4)
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degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
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cloning a PCR product. Taq pol or High Fidelity pol? - (reply: 6)
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CLONING PROBLEM!!! - no DNA after ligation!!!!! (reply: 6)
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TOPO TA cloning advice - (reply: 4)
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Have you ever re-use cloning vector from plasmid? - Wondering if it is possible to make cloning vector by remove insert (reply: 1)
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I have cloning problems with pGEM-T Easy - (reply: 5)
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blunt end cloning - (reply: 4)
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How can multiple cloning sites (MCS) NOT disrupt lacZ gene? - (reply: 2)
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expression cloning in TOPO TA and pET vectors - Very high and unusual non specific amplification in colony PCR (reply: 1)
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How to retrieve precursor miRNA sequence with extra bases for cloning - (reply: 5)
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question about blunt ligating Taq-amplified PCR product - (reply: 2)
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blunt-end / 3´A overhang cloning - (reply: 1)
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bisulfite sequencing cloning problem (methylation of DNa) - bisulfite sequencing cloning problem (methylation of DNa) (reply: 3)
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3x flag tagged cloning -> western -> multiple bands but not non-specific - Please help me!! (reply: 1)
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Cloning -> expression -> i see multiple bands :( - overexpression of my 3x flag tagged protein shows 4 bands.. (reply: 5)
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cloning - (reply: 6)
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Can't seem to do a simple cloning - (reply: 2)
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Cloning into pIBV5-His (not working) - (reply: 1)
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Cloning from cDNA - the wrong gene?! - (reply: 2)
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TA cloning and C-terminal tag - does this work!!! - (reply: 1)
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circular or linear pDNA for stable clone generation? - (reply: 1)
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TOPO TA cloning problem - (reply: 12)
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cloning problem - (reply: 2)
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Curious How they do the vector - regarding pTZ57R cloning vector (reply: 2)
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serious problem in cloning - (reply: 2)
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Cloning Problem (cells grow but no plasmid isolated) - (reply: 13)
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Cloning using BamH1 and HindIII - (reply: 8)
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minimum amounts of insert and vector for ligation - to have a successful cloning? (reply: 7)
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TA cloning necessary? - (reply: 2)
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Protein cloned in pRK-5 Myc and pcDNA3.1 Myc-His Tag not showing up - I need help for a transient expression system (reply: 3)
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cloning - how to clone a gene having stop codons (reply: 4)
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Cloning advice needed - (reply: 4)
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pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
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subcloning problem - inframe fusion (reply: 1)
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ta cloning of pfu amplified fragment - (reply: 9)
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Double digestion and cloning - (reply: 2)
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cloning of long fragment - (reply: 1)
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AAARGHHH, cloning driving me insane, HELP PLEASE! - UPDATE on same 5' and 3' ends (reply: 2)
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cloning of cellulose synthase gene - (reply: 1)
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problem in ligation and cloning - (reply: 1)
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cloning of small double strande DNA - I NEED HELP (reply: 3)
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Please Help: human Sox2, Oct 4 promoter region cloning - (reply: 2)
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concentration for cloning - (reply: 1)
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cDNA clone - (reply: 1)
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cloning of non-coding region for protein expression - protein expression (reply: 1)
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Insert Sequence Problem in Cloning - (reply: 2)
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insert sequence in clone - (reply: 6)
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Help: incompatible RE site subcloning - (reply: 1)
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need help cloning cdna of a known protein - (reply: 1)
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cloning in pET 30 B - (reply: 1)
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SacI enzyme trouble - Is this a good enzyme for cloning? (reply: 2)
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Trouble with subcloning - (reply: 6)
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about pre-miRNA cloned into lentivirus vectors - (reply: 4)
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Cloning Problem - (reply: 3)
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cloning large insert in pGEM-T easy vector - Does TA cloning work for big inserts? (reply: 2)
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What is the type of replicon from pGC blue plasmid? - Cloning (reply: 2)
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problem with cloning PCR - can't amply the full-length cDNA with PCR (reply: 6)
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Promoter Cloning for Luciferase Assay - (reply: 3)
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having the wrong vector in my clone - (reply: 3)
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cloning protocol - (reply: 1)
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Problem cloning bisulfite PCR BSP product - (reply: 6)
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cloning problems in C. elegans expression plasmid - Trying to use Fire vector pPD49.83 (reply: 1)
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problem with my clone - (reply: 3)
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Cloning and Expression Method Survey - Get some ideas from you all (reply: 1)
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cloning in Bacteroides - (reply: 9)
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Cloning woes - Running out of ideas! - (reply: 7)
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Insert problems with TA cloning - (reply: 1)
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Unstable clone? - (reply: 1)
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Problems facing in cloning with pBI121 vector - (reply: 1)
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Cloning into pGem - Problems with 5kb insert (reply: 3)
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PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
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Free trial for BxSeqTools: Online Sequence Analysis and Cloning software - free trial for BxSeqTools: Online Sequence Analysis and Cloning (reply: 1)
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Failed TA cloning with Fusion PCR product! - I need help with TA cloning of an Overlap PCR product (reply: 1)
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lentiviral system: cloning strategy - (reply: 2)
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Difficulties cloning by PCR - (reply: 5)
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problem with subcloning, please help! - (reply: 8)
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Analyse mutant clone - (reply: 7)
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Cloning by multiple oligo ligation - (reply: 2)
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anyone working currently on gateway cloning???? - (reply: 1)
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One Shot® ccdB Survival™-T1R Chemically Competent Cells: Gateway cloning???? - problems using competent cells for destination vectors in gateway clon (reply: 5)
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Cheap cloning options - any ideas????? (reply: 2)
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Subcloning - (reply: 3)
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ds-oligos cloning problems - 90bp oligo and 12kb plasmid (reply: 2)
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Subcloning into pMIR-REPORT-luc - (reply: 7)
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Cloning big fragment into smaller vector - (reply: 3)
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template for PCR for cloning purposes, miniprep or maxiprep? - (reply: 3)
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Difficulty cloning vector with multiple Lox sites - (reply: 2)
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Transcription of double blunt end Clone - (reply: 2)
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Cloning big plasmids / triple ligation - hellpppppppp (reply: 7)
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Gateway cloning - (reply: 8)
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T A cloning problems - (reply: 3)
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Have anyone ever to use miRNASelect™ pEGP-miR Cloning and Expression Vector (CEL - (reply: 1)
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Blunt-end cloning problem - (reply: 6)
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problem in gene cloning - (reply: 4)
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In-fusion advantage PCR cloning kit - --- failure after many many months-- (reply: 13)
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can't make the cloning work - (reply: 3)
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Cloning a whole gene in pBAD (any comments pls) - (reply: 4)
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cloning help - (reply: 9)
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Ligation/transformation/plates problems - molecular cloning problems (reply: 14)
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gateway cloning - (reply: 3)
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Purifying and cloning 69nt product - (reply: 5)
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Cloning strat...Awful or ok? - I use a specific strategy would love some input into how good/bad (reply: 10)
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InsTA Clone t/a cloning blues( actually whites) - (reply: 2)
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A gateway cloning expression vector question - (reply: 1)
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Promoter/gene cloning question - (reply: 7)
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File format for plasmids in Vector NTI, Clone Manager etc - I'd like a compatible format with no/minimal data loss (reply: 3)
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insert is missing after TA cloning - TA cloning (reply: 6)
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cloning problem - molecular cloning (reply: 1)
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Cloning problems - 16Kb and 1.7Kb cloning problems (reply: 5)
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Subloning problems - 1.7kb and 10kb cloning problems (reply: 3)
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wrong bands after T-vector cloning - (reply: 3)
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Help with cloning - (reply: 6)
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Help for T-A cloning - (reply: 15)
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No colony for oligo cloning - (reply: 9)
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blunt end ligation problem - (reply: 2)
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blunt ligation - (reply: 3)
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pENTR TOPO cloning - (reply: 1)
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yet one more cloning problem - (reply: 7)
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cloning miRNA library - (reply: 4)
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olligo annealing for shRNA cloning - (reply: 20)
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cloning issue - (reply: 1)
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Cloning dilemma - (reply: 3)
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How to Clone a 2.3 Kb Gene with PCR - (reply: 2)
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Cloning Problems! Need Help! - (reply: 3)
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in frame problem after 3 months of cloning! - can I stil use my construct (reply: 5)
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Cloning done....sequence of the insert for the expression vector - (reply: 1)
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cloning problem - (reply: 3)
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Cloning Conundrum - (reply: 7)
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TOPO cloning problem - (reply: 15)
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Please Help with Lentiviral Cloning - (reply: 1)
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Help with cloning!. - Cloning problems (reply: 20)
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Cloning PCR fragment - (reply: 1)
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molecular cloning - (reply: 2)
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DNA cloning - (reply: 4)
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Who can explain this? Weird cloning problem.... - (reply: 6)
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Help cloning!!! - As usual cloning is not working! (reply: 11)
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pSuper cloning problems - (reply: 1)
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Gateway Cloning Problem - Lawn of E. Coli Cells - (reply: 4)
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Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
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Receptor discovery/cloning - (reply: 1)
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Please anybody can explain the cloning related to plant vector construction - Plant vector construction (reply: 5)
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New Technologies Real time PCR, cloning, microarray, sequencing - (reply: 3)
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annoying PCR cloning problem (season 2) - (reply: 5)
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Cloning Issues with Rest Digest/Ligation/Transformation - (reply: 2)
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an annoying cloning - (reply: 9)
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Controls for TOPO cloning? - (reply: 1)
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Please help Cloning issues with smaI and pUNK1 vector - (reply: 2)
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Problems cloning Hairpin vector - (reply: 3)
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different digestion pattern for same clone - (reply: 4)
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cloning trouble - (reply: 15)
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cDNA cloning problem - (reply: 1)
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How Gateway cloning system works? - (reply: 1)
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ds-oligo cloning/ligation issues - (reply: 18)
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Cloning multiple miR30 based shRNAs into a Lenti - (reply: 2)
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vector purification (for cloning) - I used Qiagen kit and got really low yields! (reply: 7)
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Help with cloning a plasmid that contains repeats! - (reply: 2)
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questions about TOPO cloning - (reply: 7)
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Subcloning into pBluescript - (reply: 6)
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How to control cloned cDNA expression level in cells? - (reply: 8)
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Cloning a labeled PCR product - (reply: 1)
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PCR and cloning - (reply: 14)
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Cloning trouble - (reply: 5)
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Problem cloning oligo into double digested vector - (reply: 9)
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cloning 1052bp fragment - no colonies (reply: 11)
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cloning problems - empty vector (reply: 3)
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Cloning purified PCR products eluted in Qiagen's EB - Concentrate a purified PCR product eluted with EB? (reply: 11)
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cDNA cloning & expression - use ORF or full-length cDNA? - (reply: 5)