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Cell Related Discussions

1. Correlation between microorganism division rate and cell dimorphism - (reply: 1)
   
2. Help! Cell contamination ? - (reply: 1)
   
3. cell culture medium - (reply: 1)
   
4. Does the volume of buffer you resuspend a pellet in affect cell yield? - (reply: 1)
   
5. Inhibition of SARS-CoV-2 in Vero cell cultures by peptide-conjugated morpholino- - (reply: 8)
   
6. Generating a cell line using sleeping beauty transposase and a gene that contain - (reply: 1)
   
7. Adherent cells detach themselve from petri dish (problems in cell culture) - (reply: 6)
   
8. HELP: Validation of a polyclonal knock out (KO) cell line when you dont have a g - (reply: 6)
   
9. expressing bacteriophage in mammalian cells - (reply: 5)
   
10. cell free DNA (cfDNA) - (reply: 1)
    
11. Contamination of cell culture - Weird stringy thing in cell culture flasks - (reply: 2)
    
12. How to calculate cell viability percentage from the absorbance in LDH assay? - (reply: 2)
    
13. what is mean by Mock cell? - (reply: 1)
    
14. What is going on with these cells? - (reply: 3)
    
15. Can glucose-6-phosphate enter human cells? - (reply: 2)
    
16. Electrophoresis of proteins from cells - (reply: 9)
    
17. Issue about reusing cells - (reply: 1)
    
18. cell line authentication - (reply: 1)
    
19. MNFS60 cells are dying. - (reply: 2)
    
20. De-clumping cells - (reply: 1)
    
21. Cell Biology: Transcription - (reply: 2)
    
22. Why does cell division, as asexual reproduction, perpetuate life of unicellulars - (reply: 4)
    
23. Releasing cell associated virus - (reply: 14)
    
24. Two distinct populations observed on a homogenous cells - (reply: 1)
    
25. Help novice with question about cell concentration in batch culture - (reply: 3)
    
26. Cell counting - (reply: 12)
    
27. HeLa cells dangerous? - (reply: 4)
    
28. black spots are found in cells and medium - (reply: 1)
    
29. Unidentified geometric objects in cell culture - (reply: 3)
    
30. Cell Membranes as an active barrier - (reply: 1)
    
31. RNA degradation from cells embedded in gels - (reply: 4)
    
32. anti-HA immunofluorescence on HEK 293 cells - (reply: 5)
    
33. restoring melanocyte stem cells in gray hair - (reply: 10)
    
34. Normalise cell count for protein analysis - (reply: 3)
    
35. Blockage of cadherin bindings of MCF-10A cells without harming hydrogels and cal - (reply: 2)
    
36. 293T cells not able to attach well after passage - (reply: 1)
    
37. Decrease of total cells in a stirred bioreactor cultivation? - (reply: 1)
    
38. Reorganization of liquid nitrogen cell stocks - (reply: 1)
    
39. Should Lineage marker cocktail be used in cell lines to remove lineage specific - (reply: 1)
    
40. How to make normal cells cancerous - (reply: 1)
    
41. 293T cells unable to revive - (reply: 5)
    
42. Counting number of cells - (reply: 1)
    
43. What is the source of contamination in my cell culture? - (reply: 2)
    
44. Stem cell imaging without a need for fluorescence: win a microscope (deadline No - (reply: 1)
    
45. prepare competent cells from existing competent cells - (reply: 2)
    
46. slimy cell paste of recombinant e.coli - (reply: 3)
    
47. Cell Counting - (reply: 1)
    
48. Cell cycle - (reply: 2)
    
49. How long do you reckon a cell pellet can survive before it must be resuspended? - (reply: 1)
    
50. ¿must treatment be aplied 24h post seed the cells? - (reply: 1)
    
51. Question about transfecting siRNA into cells - (reply: 1)
    
52. Mammalian Cell Lysis - (reply: 1)
    
53. 293T cells for lentivirus production - (reply: 1)
    
54. Counting and seeding cells?]. - (reply: 1)
    
55. propagating commercial chemically competent cells - (reply: 2)
    
56. CRISPR-Cas9 / insert a gene in cell line genome - (reply: 1)
    
57. Beginner Cell and Tissue, and Molecular Biology methods reading list - (reply: 1)
    
58. how to present Michaelis-Menten equation in cell designer - (reply: 1)
    
59. TCDD (2,3,7,8-​Tetrachlorodibenzo-p-dioxin solution) cell line treatment - (reply: 1)
    
60. cell invasion assay - (reply: 1)
    
61. Help with the Red Blood Cell Lysis - (reply: 1)
    
62. gene expression in cultured cell lines - (reply: 1)
    
63. Splitting cells without centrifugation - (reply: 4)
    
64. Are HEK293 cells a positive control for wild type p53 expression? - (reply: 1)
    
65. Culturing LoVo cells - (reply: 1)
    
66. Black dots contaminant in stable cell lines - antibiotics - (reply: 1)
    
67. cell viability assay for caco-2 and pbmc - (reply: 1)
    
68. 3T3 L1 cell culture - (reply: 1)
    
69. Why my bacteria cells look weird in DAPI fluorescence? - (reply: 2)
    
70. B & T cell activation in autoimmune disorders - (reply: 1)
    
71. lentiviral transduction in BLCL cells - (reply: 3)
    
72. Synchronization of HEK293T cells for IP - (reply: 2)
    
73. Alternate cell line not giving same results as first - (reply: 1)
    
74. Cell volume and reciprocal cell volume? Whaaat? - (reply: 1)
    
75. How to detect specific-protein producing cells? - (reply: 8)
    
76. Who came up with 50-100 IU/mL Antibiotic working concentration in cell culture? - (reply: 2)
    
77. Help! Antibiotic and Serum Concentration in Cell Culture Media - (reply: 1)
    
78. Large-scale cell growth and protein expression - (reply: 1)
    
79. is cell signaling difficult? - (reply: 3)
    
80. What would be the shortest and optimal method of extracting human cells for PCR? - (reply: 2)
    
81. The adipose cell culturing - (reply: 2)
    
82. Recommendation of cell signaling inhibitors - (reply: 2)
    
83. Recovering cells from ancient sample - (reply: 3)
    
84. Microinjection of cells into the lumen of spheroids ? - (reply: 1)
    
85. Human cell membrane protein Western blot! - (reply: 2)
    
86. Cell Line confluence level - (reply: 1)
    
87. too much cell density can influence adhesion to culture plate - (reply: 1)
    
88. Shipment conditions for cultured cells - (reply: 3)
    
89. pIKK antibody for western blot (RAW 264.7 cells) - any recommendations? - (reply: 1)
    
90. E-Cadherin Expression in OAW-42 Cells - (reply: 1)
    
91. Why HepG2 cells looked like that? - (reply: 2)
    
92. Trouble transfecting HEK 293 T and HUH 7 cells - (reply: 1)
    
93. 3T3-L1 cell contamination - (reply: 3)
    
94. Will lipofection of DNA work in post mitotic cells - (reply: 1)
    
95. Cell death determination - (reply: 2)
    
96. Culture Tubes for CHO cells - (reply: 1)
    
97. SHSH5Y cells - (reply: 1)
    
98. Is the mycoplasma that infects cell culture dangerous to humans? - (reply: 1)
    
99. Distinguishing two populations of cells by TEM - (reply: 4)
    
100. transfection of suspended CHO cells - (reply: 2)
     
101. using IC50 to determine dosing of inhibitor in cell culture - (reply: 2)
     
102. Software for automated cell counting? - (reply: 2)
     
103. why is my cells dying in the centre of the wells after transfections - (reply: 1)
     
104. How to establish drug-resistant cell lines? - (reply: 1)
     
105. Are normal or immortalized human epithelial cells clonogenic? - (reply: 1)
     
106. BJAB cells: does anyone have a specific protocol for transfection? - (reply: 1)
     
107. Fibroblast Cell Culture Issue - Cells grouping together and cell clusters observ - (reply: 5)
     
108. pUC and pSC101 vectors in the same cell - (reply: 3)
     
109. Jurkat cell - normal receptor/gene expressivity - (reply: 1)
     
110. Can propidium iodide be used on WBC nuclei with cell membrane removed - (reply: 1)
     
111. Inactivating effects on immune cells caused by mutation in the gene encoding the - (reply: 2)
     
112. Is cultured cell reuse possible? - (reply: 1)
     
113. Count cell bactria using haemacytometer - (reply: 1)
     
114. Help with HUVEC cell transfection - (reply: 3)
     
115. Cell clumps or contamination? Please help! - (reply: 1)
     
116. How to clean up cell fragments before cell sorting - (reply: 1)
     
117. Is any other method to maintain without changing media, or store cell culture fo - (reply: 2)
     
118. MDA-kb2 cells - problem with luciferase expression - (reply: 6)
     
119. Cell lysis method to get "intact" nuclear protein complex for CO-IP? - (reply: 1)
     
120. Abnormality in PMA treated THP1 cells - (reply: 1)
     
121. Preferred assay for measuring cytotoxicity or cell proliferation - (reply: 2)
     
122. Cell lysis - (reply: 1)
     
123. Can foam created when resuspending a cell pellet cause cell demage - (reply: 1)
     
124. Distribution of cell receptors on the cell surface - (reply: 3)
     
125. MTT Cell Treatment - (reply: 1)
     
126. cells behaving differently - (reply: 4)
     
127. Cell lines behaving differently to drugs - (reply: 2)
     
128. How to achieve a uniform monolayer with HepG2 cells, seeded in 24 welll plate fo - (reply: 1)
     
129. Efficient lysis of E.coli cells - (reply: 4)
     
130. Alternative competent cells for QuikChange kit - (reply: 2)
     
131. unknown bodies in cell culture - (reply: 1)
     
132. Unbroken cell - (reply: 1)
     
133. how to confirm lentiviral integration in a target cell line - (reply: 1)
     
134. Invasion assay: analysis question: round cells - (reply: 2)
     
135. Measuring ammonia in cell lysates - (reply: 1)
     
136. Methanol oor PFA to fix cells for nuclear protien analysis by immunofluorescence - (reply: 4)
     
137. What happens when trypsin is added to cells? - (reply: 1)
     
138. Selective evolution of cells for cancer - (reply: 4)
     
139. cell line - (reply: 3)
     
140. I want know what companies are producing Biosimilar cell line - (reply: 1)
     
141. contamination in HepG2 cell culture - (reply: 1)
     
142. Which neuroblastoma cell lines grow the longest axons - (reply: 1)
     
143. Help with Question for Cell Bio Take Home Exam - (reply: 2)
     
144. leukemic cell lines 697 - (reply: 1)
     
145. where do you get cells needed for your research - (reply: 1)
     
146. PCR with mammalian cells as template - (reply: 1)
     
147. Dislodge cell in 96well plate - (reply: 1)
     
148. The right lentivirus plasmid for overexpression in human cells - (reply: 7)
     
149. cell counting - (reply: 2)
     
150. What do the glioma cell line prefixes LN and U stand for ? - (reply: 1)
     
151. colorectal cell culture - (reply: 2)
     
152. Easy to transfect primary cell line? - (reply: 1)
     
153. Sample prep of cells for FACS analysis and running WB - (reply: 4)
     
154. Functional states of the same cell versus Different cell types - (reply: 2)
     
155. How long after transfection or dox induction of stable cell line before performi - (reply: 1)
     
156. transfection of cells with two fluorescent reporters - (reply: 1)
     
157. Stably transfected cells start dying - (reply: 6)
     
158. U251 and U87 cells - (reply: 1)
     
159. is -40 freezer enough for cell freezing? - (reply: 1)
     
160. Experiment on nutrient surplus on Jurkat cell culture - (reply: 2)
     
161. Help with HeLa cell cultivation - (reply: 5)
     
162. skin lesion and low donor cell frequency in bone marrow transfer - (reply: 7)
     
163. cell division rates - (reply: 6)
     
164. Black dots-ghost cells or contamination? Please help - (reply: 6)
     
165. Where do you purchase T cell hybridomas - (reply: 5)
     
166. NK Cell Culture Selection and Expansion from PBMC - (reply: 2)
     
167. 3D Cell Culture Matrix made of jelly / gelatin - (reply: 2)
     
168. Cloning purified PCR product into cells - (reply: 5)
     
169. Help ID fluoescently-labelled cells in the gut very much appreciated! - (reply: 2)
     
170. Mention of Transgene Insertion into Cell Lines By Transfecting with cDNA - (reply: 2)
     
171. Refreeze thawed cells? - (reply: 3)
     
172. How can an animal cell obtain needed glucose when glucose is lacking in the diet - (reply: 2)
     
173. Suppose a microorganism invades a cell, causing damage to ATP synthase. How woul - (reply: 3)
     
174. How to capture live cells under microscope - (reply: 3)
     
175. Identification of cells and tissue - (reply: 2)
     
176. Research topic related to jurkat cell line - (reply: 3)
     
177. What DCH5a cells are? / pCAMBIA1301 isolation from DHC5a cells - (reply: 3)
     
178. When is it suitable for using NIH3T3 cells to validate cancer genes? - (reply: 1)
     
179. Assays from very few number of cells - (reply: 1)
     
180. What I have to do If my active compound showing toxicity in normal cells? - (reply: 1)
     
181. Passaging cells - (reply: 1)
     
182. Problem in mouse embryonic stem cell culture - (reply: 4)
     
183. Is there always a presence of activated T cells in a healthy host? - (reply: 1)
     
184. Bacterial contamination in cell culture - (reply: 3)
     
185. Cell culture contamination - (reply: 1)
     
186. Contamination or dead cells? - (reply: 1)
     
187. How to choose cell line - (reply: 4)
     
188. Urea effect on mice spleen cells - (reply: 3)
     
189. Urea effect on mice spleen cells - (reply: 1)
     
190. Trypan Blue if cells cultured in 96 well plate - (reply: 2)
     
191. Best cryovials for liquid nitrogen storage of cells? - (reply: 2)
     
192. Review articles on all types of mammalian cell culture - Any suggestion? - (reply: 1)
     
193. 2 types of cell lines for RNA extraction - (reply: 1)
     
194. live cell culture transport in 6 well plate - (reply: 6)
     
195. Stem Cell Culture Theory - (reply: 4)
     
196. Selectively arresting a subpopulation of biotinylated yeast cells. - (reply: 4)
     
197. cell viability with PI staining - (reply: 2)
     
198. Sonication of cells in ultrasound bath, without probe - (reply: 3)
     
199. cell fractionation and microscopy - (reply: 3)
     
200. Cell Cycle Analysis - crucial to keep cells cold after ethanol fixation, and aft - (reply: 1)
     
201. cell lysis problem - (reply: 2)
     
202. Made a mistake and fixed cells with 30% ethanol for cell cycle analysis. Can I s - (reply: 1)
     
203. Thawing cells - (reply: 2)
     
204. the same construct, two stable cell clones with different protein size - (reply: 2)
     
205. Determine DNA sequence expression in cell line - (reply: 1)
     
206. Harvesting cells - (reply: 3)
     
207. Cell counters - Beckman Coulter Z1 counter - (reply: 1)
     
208. Competent cells with Hanahan's method - (reply: 2)
     
209. problem with cell synchronization - (reply: 4)
     
210. can anyone tell me what kind of cell this is? - (reply: 3)
     
211. Transfection of SH-SY5Y cells - (reply: 4)
     
212. Protocol for isolating cells from Subcutaneous Tumors ? - (reply: 1)
     
213. Hybridoma cells contaminated with large round cells! - (reply: 1)
     
214. protocol for cell syncronization using colchicine - (reply: 2)
     
215. PBMC cell stimulation? - (reply: 1)
     
216. Caco-2 cells with unidentified contaminant - (reply: 2)
     
217. MIN6 Cell Culture Contamination Risk - (reply: 1)
     
218. Concentrating bacterial cells - (reply: 2)
     
219. Would preserved cells for flow cytometry make your life easier? - (reply: 1)
     
220. measure of cell size - (reply: 1)
     
221. Transfecting cells for cell cycle analysis, how to deal with cell density? - (reply: 1)
     
222. Splitting Cells that are shaped as small spheres - (reply: 2)
     
223. Cells counting problem. - (reply: 2)
     
224. Cell counts for Clonogenic and MTT assay - (reply: 3)
     
225. Do I need to use untreated TC flask for suspension cell culture - (reply: 5)
     
226. Cell cycle determination - (reply: 1)
     
227. After centrifuge in serum-free media, forming cell sheet, instead of pellet in t - (reply: 2)
     
228. Can cells be exposed to nanoparticles with ethanol as solvent??? - (reply: 2)
     
229. Question about growth media for A20 Cell Line - (reply: 1)
     
230. NSO cell culture - (reply: 1)
     
231. Some queries regarding A549 cell culture - (reply: 1)
     
232. Has anyone experience in isolating single-cell clones of CHO transfected cell li - (reply: 4)
     
233. how to transport stem cells in suspension? - (reply: 2)
     
234. animal cell culture - (reply: 1)
     
235. help with contamination of cell culture - (reply: 2)
     
236. Methanol concentration for cells? - (reply: 4)
     
237. What happened to my cell? (Colony formation) - (reply: 7)
     
238. problem of cell lysate - (reply: 3)
     
239. Cell lysis and DNA quantification - (reply: 1)
     
240. Best western loading control for comparing different cell lines - (reply: 2)
     
241. B cell line - cell colour change and visible cell death - (reply: 1)
     
242. Keeping apoptotic/detached cells for WB? - (reply: 3)
     
243. Effective inhibitor of cell proliferation - (reply: 4)
     
244. Lentivirus transfection using RAW 264.7 cells - (reply: 2)
     
245. Neural Stem Cells; does it mean adult or embryonic? - (reply: 1)
     
246. Cell culture contamination or not? - (reply: 1)
     
247. orthotopic mouse model of U251 cell line - (reply: 4)
     
248. How to release the bacterial cells from filter paper? - (reply: 4)
     
249. Cell adhesion/detachment - (reply: 1)
     
250. methylation observation in normal cell line - (reply: 1)
     
251. Stem cells created by dipping researchers into coffee - (reply: 2)
     
252. Freezing yeast cells - (reply: 4)
     
253. codon optimization for CHO cells - (reply: 2)
     
254. FITC conjugated phalloidin staining of cells on microcarrier beads - (reply: 1)
     
255. chk effect of cell line genes on normal dna - (reply: 1)
     
256. Would someone help me discuss what work can I do with laryngeal cancer cell hep2 - (reply: 12)
     
257. DAPI stainged dots only in cells transfected with Fugene6 - (reply: 5)
     
258. 293T cell transfection troubles - Lipofecamine 2000 - (reply: 3)
     
259. Can I make my own electrocompetent Stbl4 cells? - (reply: 2)
     
260. CHIP on frozen cell pellet - (reply: 1)
     
261. UV Light benefits and costs on Cells - (reply: 2)
     
262. Adapting a Drosophila cell culture to Zebrafish for subsequent MACS-sorting - (reply: 3)
     
263. mouse embryonic stem cell culture on gelatine coated plates - (reply: 1)
     
264. easiest cell lines to use - (reply: 4)
     
265. Jurkat cell - (reply: 4)
     
266. Mesenchymal stem cells Expressing BDNF - (reply: 5)
     
267. PI staining cell cycle analysis - (reply: 1)
     
268. Protein Purification from mammalian cells - (reply: 1)
     
269. How to identify CD8 T cell receptor for a particular antigen - (reply: 2)
     
270. How to inactivate H1N1 virus in Cell culture supernatant ? - (reply: 3)
     
271. HaCaT cell line - not responding to inflammatory stimuli such as LPS/TNF-alpha - (reply: 4)
     
272. Homogenous Cell Lysates - (reply: 1)
     
273. Does anyone irradiate their cells? - (reply: 3)
     
274. need help for transfection to synchronized cells - (reply: 4)
     
275. If MCF-7 cell line can expresses Her-2 gene? - (reply: 5)
     
276. What should I suspend my cells in prior to cytometry? - (reply: 2)
     
277. Checking for efficieny competent cells - (reply: 1)
     
278. Video on cell culture technique (broad brush) - (reply: 2)
     
279. Setting up two drug kill curve for selection of stable cells - (reply: 1)
     
280. No expression in stable transfected cell line - (reply: 2)
     
281. Protocol for flow cytometry on mononuclear cells from mouse blood - (reply: 1)
     
282. Cell line contamination? - (reply: 5)
     
283. Inducing apoptosis by puromycin selection: toxic to the selected cells? - (reply: 1)
     
284. Measuring cell proliferation from daily aliquots - (reply: 1)
     
285. Cell culture of MYC immortalised HDFa - need advice - (reply: 1)
     
286. Phoenix Amphoteric cell line to package lentivirus? - (reply: 6)
     
287. miR30 based shRNA in mouse cells - (reply: 3)
     
288. Cells are unhealthy - (reply: 2)
     
289. Harvesting cells - (reply: 12)
     
290. Dead cells after transfection - (reply: 9)
     
291. preparation of cells for FACS analysis after transfection - (reply: 4)
     
292. simultaneous knock down of two genes in a single cell line - (reply: 1)
     
293. CoIP from cell lysate mix - (reply: 5)
     
294. selective pressure on cells - (reply: 2)
     
295. Conjugation of Ab to magnetic beads to isolate T cells from Spleen - (reply: 2)
     
296. cell culture and biopsy - (reply: 1)
     
297. Tiny black dots inside cell vacuoles - (reply: 1)
     
298. I always have a cell depris floating in the supernatent of MDCK cells - (reply: 3)
     
299. Is Trifluoroacetic acid toxic for cells? - (reply: 5)
     
300. Treating suspension cells - (reply: 2)
     
301. H9c2 cells..please help - (reply: 2)
     
302. loading control issue in blot with H9c2 cell protein, pleeeeeasee help - (reply: 2)
     
303. How can i seed 2000 cell /well? (Using 200 microliter media and 96 plate, and as - (reply: 1)
     
304. Establishing human neural stem cells - (reply: 3)
     
305. Immunofluorescence Staining Problem of Mitotic Cells - (reply: 2)
     
306. Cell Adhesion and Motility on Fibronectin vs Collagen - (reply: 1)
     
307. FCS in Serum free medium for better Cell Adhesion? - (reply: 1)
     
308. Possible to sort cells from formalin? - (reply: 1)
     
309. 293s vs HEK/293 cells - (reply: 2)
     
310. Cell culture medium contamination? - (reply: 1)
     
311. What do sick 293t cells look like? - (reply: 3)
     
312. H2DCFDA and fixation of cells before flow cytometry analysis - (reply: 4)
     
313. Decreasing cell division - (reply: 1)
     
314. Can I use methanol fixation to fix cells before proteomic analysis? - (reply: 1)
     
315. Is it generally acceptable to ask another lab for cell line/plasmid/etc - (reply: 4)
     
316. Do S2 cells normally have small 1-2um objects floating in culture? - (reply: 1)
     
317. Problem in Cell Viewing - (reply: 3)
     
318. How to view the encapsulated cells under microscope? - (reply: 5)
     
319. Poor data quality using cultured cell in aCGH - (reply: 10)
     
320. Immunohistochemical estrogen/progesterone receptor staining for CELLS - (reply: 1)
     
321. Totipotent Stem Cell From Molecular Components - (reply: 5)
     
322. How to count clumpy epithelial cells? - (reply: 5)
     
323. Why cells are floating after incubation in EBSS?? - (reply: 1)
     
324. purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
     
325. Live cell sort DNA stain permeabilization - (reply: 1)
     
326. Problem in bacterial cell culture - (reply: 12)
     
327. Persistent contamination in cell culture - (reply: 9)
     
328. PE and FITC stain after fixing cells with 4% PFA - (reply: 3)
     
329. Adult stem cells - (reply: 3)
     
330. Centrifuge stopped working while my blood samples were incubated in Cell lysis s - (reply: 1)
     
331. Ways to Quickly Identify Antibiotic Resistance in Bacterial Cells (Non-tradition - (reply: 12)
     
332. freezing competent cells without dry ice or liquid N - (reply: 3)
     
333. Suspension cell line (RPMI) adhere to flask-WHY? - (reply: 1)
     
334. Rapamycin Concentration for Cell Culture - (reply: 1)
     
335. how to calculate cells and plate - (reply: 2)
     
336. yeast conatamination in primary cells - (reply: 4)
     
337. Maltodextrin will kill all the bacterial cells? - (reply: 5)
     
338. Keratinocyte Culture: Cells detach - (reply: 7)
     
339. Measure growth of plant cell culture - (reply: 1)
     
340. Cell Lysate Western Blot Little or No Signal - (reply: 7)
     
341. Cell line identity (HEK293-E) - (reply: 1)
     
342. How do I calculate the enery absorved by cells form a UV light - (reply: 3)
     
343. Immunocytochemistry - Washing away PC12 cells - (reply: 2)
     
344. Protein secretion wtih pFuse expression system in 293T cells - (reply: 2)
     
345. Total Protein in COS-7 Cells - (reply: 1)
     
346. protein extraction from cell nucleus - (reply: 2)
     
347. Which gauge for proper shear force on certain cell dimension - (reply: 1)
     
348. Mammalian Cell Lysis Buffer Triton-X100 Content - (reply: 6)
     
349. DNA from frozed cells??? - (reply: 2)
     
350. SH-SY5Y stable cell transfection and RA differentiation - (reply: 1)
     
351. Tumor cell analysis - (reply: 2)
     
352. What can I do with my MCF7 cells? - (reply: 1)
     
353. Evolution of Living Cells from Non-living Organic Matter - (reply: 3)
     
354. cell lines - (reply: 5)
     
355. M2 antibody background in Xenopus cell extracts - (reply: 6)
     
356. Asking for guidelines to start L6 cell line culturing - (reply: 1)
     
357. how to separate organelles from mammalian cells - (reply: 1)
     
358. Cell culture contamination ? - (reply: 5)
     
359. How to coat 96 well plate with poly-L-Lysine for cell culture use? - (reply: 2)
     
360. Is Protein A present on the Staphylococcus saprophyticus cell wall too? - (reply: 2)
     
361. Protein bandshift in western blot of cell lysate vs immunoprecipitation eluent - (reply: 4)
     
362. red dots in cell culture line? - (reply: 3)
     
363. Integrity of cell monolayer - (reply: 1)
     
364. problem with COV318 ovarian cancer cell line - (reply: 4)
     
365. Expansion of commercial competent bacterial cell lines? - (reply: 2)
     
366. atom replacement in human cells - (reply: 2)
     
367. What stage of growth are the cells I counted? - (reply: 4)
     
368. HeLa cell culture - (reply: 6)
     
369. Lambda Phosphatase Treatment of Cell Lysate - (reply: 1)
     
370. Problem in Determination of Total Bacterial Cells - (reply: 13)
     
371. Preparing a cell culture stock to plate out at 7x10^5 per well? - (reply: 1)
     
372. Cell culturing tips - (reply: 2)
     
373. SAOS.2 Cell detachment without using Trypsin. - (reply: 3)
     
374. Normal cells or contamination?! - (reply: 4)
     
375. H2DCFDA for MDCK cells - (reply: 2)
     
376. immortalized human neuronal cells - (reply: 4)
     
377. LNCaP Cell Clusters - (reply: 2)
     
378. Freezing viable cells in 96-well format? - (reply: 3)
     
379. question about sequential transfection several plasmids in 293T and COS7 cells - (reply: 1)
     
380. unidentified objects in my cells, no staining.. what are they? - (reply: 2)
     
381. Does fixation and permeabilization of cells affect their oxidative stress levels - (reply: 1)
     
382. HBE cell culture CRL-2079 MEDIA question - (reply: 1)
     
383. Cells dying in culture after 24 hours - (reply: 6)
     
384. SH-SY5Y cells: basics advice - (reply: 1)
     
385. Cell clumping: dead cells? - (reply: 6)
     
386. What is your favorite method for making chemically competent E.coli cells? - (reply: 7)
     
387. growing cometent cells - (reply: 1)
     
388. Where can I purchase HepG2.2.15 cell line - (reply: 1)
     
389. RAW 264.7 cell line - (reply: 1)
     
390. Mycoplasma infected cell line clean-up - (reply: 1)
     
391. Rheumatoid Factor (RF) producing cell line? - (reply: 1)
     
392. Weighing harvested cells - (reply: 1)
     
393. Temperature Variation on Cell Growth Experiment - (reply: 1)
     
394. Morphology of cultured cells - (reply: 5)
     
395. Growing GH teratocarcinoma cell line- help needed - (reply: 2)
     
396. Cells Detach After Transfection - (reply: 1)
     
397. How to determine the location of integrated DNA in stable cell lines - (reply: 2)
     
398. No reduction in protein expr after 2nd passage of transfected cells - (reply: 3)
     
399. When cell media becomes "used up", it acidifies - (reply: 3)
     
400. Cell cycle analysis for SubG1 fraction - (reply: 3)
     
401. Clone selection for scrambled shRNA transfected cells ? - (reply: 2)
     
402. Cytokines feeding cell culture - (reply: 1)
     
403. Experience with U-937 cell line - (reply: 3)
     
404. Cell culture contamination issue - unable to identify the contamination - (reply: 1)
     
405. cell confluency in IF - (reply: 2)
     
406. Protein purification for adherent mammalian cells - (reply: 2)
     
407. Antibody production in Expi293 cells - (reply: 2)
     
408. death of transfected cells after first passage in G418 medium - (reply: 1)
     
409. clonogenic assay for shRNA transfected cells - (reply: 3)
     
410. Cell lysis in chromosome conformation capture - (reply: 2)
     
411. Relationship between cell lines and cancer research (IHC) - (reply: 4)
     
412. Serum starvation - something similar for serum-free kept cells ? - (reply: 2)
     
413. Sodium bicarbonate addition to GMEM for BSR T7/5 cells - (reply: 2)
     
414. No proteins after HeLa cell lysis - (reply: 3)
     
415. How can i fix cultured cells on glass slides for insitu hybridization - (reply: 1)
     
416. Blocking a protein in cell culture through antibody - (reply: 10)
     
417. How to propagate Electro-competent DB3.1 cells - (reply: 6)
     
418. Number of cell - (reply: 5)
     
419. Difference between PFA, acetone and methanol in cell fixation - (reply: 1)
     
420. cell proliferation statistics methods - (reply: 4)
     
421. Losing cells during fixation - (reply: 2)
     
422. ovarian cancer cell line: phenotype disappears - (reply: 3)
     
423. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
     
424. HeLa Cells on Chin/in Nose? - (reply: 7)
     
425. Can centrioles be seen when the cell is not undergoing cell division? - (reply: 3)
     
426. How much ethanol (in micro g) has toxic effects on cells? - (reply: 1)
     
427. penicillin/streptomycin in cell culture - (reply: 3)
     
428. Recombinant expression of cytokines in mammalian cells without the propeptide re - (reply: 1)
     
429. Cell lysis for unknown reasons - (reply: 1)
     
430. Preparing cells for plating - (reply: 3)
     
431. ChIP with low cell number - (reply: 2)
     
432. Mammalian cell culture and G418 selection - (reply: 1)
     
433. Losing cells during intracellular staining method! - (reply: 1)
     
434. G418 mammalian cell selection question - (reply: 3)
     
435. DIFFERENCES BETWEEN GLIAL AND GLIOMA CELL? - (reply: 4)
     
436. Bought HEK293T cells from ATCC -- passage number question - (reply: 1)
     
437. Cell lines selected with G418 but no overexpression of GOI - (reply: 3)
     
438. cell pellet question - (reply: 2)
     
439. B cells cultures. Immunoglobuline production - (reply: 1)
     
440. ChIP problem - using cells as a negative control - (reply: 7)
     
441. Blood cell migration from mother to fetus - (reply: 1)
     
442. Qiagen mini prep cell fragment pellet - (reply: 2)
     
443. Cell death detection using adenosin kinase - (reply: 5)
     
444. THP-1 differentiation using PMA yielding black dots within cell structure - affe - (reply: 4)
     
445. Transfecting three plasmids into hES cells - (reply: 2)
     
446. Agglomeration of suspension cells (T-cells) - (reply: 1)
     
447. Selection of E.coli competent cells - (reply: 2)
     
448. Chelating Aluminum from cell culture - (reply: 5)
     
449. chemotherapeutic-resistant cell lines - (reply: 1)
     
450. infection assay-buffer that lyses mammalian cells but not bacteria - (reply: 1)
     
451. HEK293 vs. SH-SY5Y Cell lines? Which best for in vitro Alzheimer's Model? - (reply: 2)
     
452. Selection of needle size to homogenize cells for RNA extraction - (reply: 3)
     
453. Electroporate lentiviral vector for stable cell line - (reply: 2)
     
454. Place to buy (soluble) ligands for cell culture. - (reply: 3)
     
455. NIH 3T3 cells cross contamination - (reply: 1)
     
456. NIH 3T3 cells - (reply: 3)
     
457. Cells adhering to walls of conical tube? - (reply: 7)
     
458. PI cell cycle analysis and apoptosis - or would a different stain be better? - (reply: 2)
     
459. choosing the correct subculturing ratio - ovarian cancer cell lines - (reply: 1)
     
460. RAW 264.7 cell line - (reply: 4)
     
461. washing of attached bacterial cells - (reply: 3)
     
462. quick and non-expensive method of testing cross cell contamination? - (reply: 4)
     
463. Cell Debris Isolation From Blood - (reply: 3)
     
464. How to calculate drug amount to use in cell lines - (reply: 3)
     
465. Growing certain number of bacterial cells in LB - (reply: 1)
     
466. T cells or Lymphocytes? - (reply: 2)
     
467. Western Blot with fixed cells - (reply: 5)
     
468. Less than 10 GFP positive cells - (reply: 2)
     
469. Doubt: Cell plating for different cell culture plate formats - (reply: 3)
     
470. How to culture HK-2 cells - (reply: 6)
     
471. competent cells - (reply: 1)
     
472. Debris in cell culture - (reply: 2)
     
473. What housekeeper to use with HEK cells? - (reply: 3)
     
474. Housekeeper for HEK cells? - (reply: 1)
     
475. Type I Collagen Vs. Poly-L-lysine Vs. Gelatin Coated coverslips (NSC34 cells) - (reply: 1)
     
476. Cell-repellent coating / surfaces - Growing cells on beads - (reply: 2)
     
477. C3HT101/2 Cell Line not attaching to T25 flask- nightmare!!!! - (reply: 5)
     
478. Phorbol myristate acetate treatment of J774 cell line - (reply: 1)
     
479. Gene expression profiling with small cell number - (reply: 1)
     
480. Gene expression assay with small cell number - (reply: 3)
     
481. Spontaneous immortalization of mouse cells in vitro - (reply: 1)
     
482. Stem Cell Therapy comes of age? Discussion Topic - (reply: 1)
     
483. cell free DNA and maintaining stability - (reply: 3)
     
484. Freezing Cells For Use In Assays - (reply: 2)
     
485. INS-1E cells migration assay - (reply: 1)
     
486. Short-term storage of HEK293 cells before FACS - (reply: 3)
     
487. Sample storage for ELISA (cell culture supernatant) - (reply: 2)
     
488. Any tips on how to avoid lifting cells in 96well plates - (reply: 3)
     
489. Primary cell line - (reply: 5)
     
490. 2D Image Primary Cell line - (reply: 13)
     
491. will mRNA from different cell line lead to loss of expected product in RT PCR? - (reply: 2)
     
492. MCF-7 Clumping - (reply: 3)
     
493. Geneticin Resistant Cell Lines and Stable Transfection - (reply: 1)
     
494. To small amount of cells used for shRNA transfection - (reply: 1)
     
495. using cell line to generate standard curve - (reply: 6)
     
496. Autofluorescence in thick structure cluster of cells - (reply: 4)
     
497. Primary culture of fibroblast cells from rat/mouse - (reply: 4)
     
498. Serial dilution of cells - (reply: 3)
     
499. Cloning repetitive sequences in mammalian cells - (reply: 3)
     
500. Do cell signaling antibodies work with 1h RT incubation? - (reply: 2)
     
501. Plant Cells - what are they? - (reply: 1)
     
502. HS-5 and HS27A cells - (reply: 1)
     
503. MC-3T3 cells are not mineralizing... - (reply: 3)
     
504. HS-5 cell culture "question" - (reply: 1)
     
505. Problem with Kasumi-6 cell line - (reply: 1)
     
506. G418(Geneticin) is not killing my cells - (reply: 2)
     
507. Community feedback needed to clarify terms for a Cell Culture Ontology - (reply: 1)
     
508. Quantify total DNA in small cell lysate...? - (reply: 1)
     
509. Trouble validating in multiple cell types - (reply: 1)
     
510. Gating of dendritic cells - (reply: 7)
     
511. Please help me take a look at my cells to see whether is it contaminated - (reply: 3)
     
512. cell binding assay with nanoparticles? - (reply: 2)
     
513. Cell lines for clonal selection - (reply: 3)
     
514. Pelleting cells by gentle centrifugation for addition to media - (reply: 13)
     
515. SH-SY5Y Immortalized cell lines; researching neurotoxicity? - (reply: 3)
     
516. Pelleting bacterial cells - (reply: 1)
     
517. How viable is cell culture for a model? - (reply: 2)
     
518. Help with adherent cell culture - (reply: 7)
     
519. study of receptors in cell lines - (reply: 1)
     
520. Cell line nomenclature - suffixes ? - (reply: 3)
     
521. Maintainence of transformed cells - (reply: 5)
     
522. improving viability of pelleted cells - (reply: 2)
     
523. Buying Ins-1e cells - (reply: 1)
     
524. How do/should cell biologists calculate power of analysis? - (reply: 1)
     
525. comparing ADCC effect in 3 cell lines. - (reply: 1)
     
526. Help: Best way to disrupt blood cells to generate blood debris and gDNA to be us - (reply: 4)
     
527. Ric3 NAChR 7 expression in HEK cells - (reply: 1)
     
528. virus and cell surface receptor interaction - (reply: 4)
     
529. Music Video on Cell Division/Mitosis - (reply: 3)
     
530. How to select competent E. coli cells? - (reply: 7)
     
531. Host cell protein sequence search - (reply: 1)
     
532. qPCR from HEK cells transfected with Plasmid - (reply: 4)
     
533. (best cell lysis method for) protein expression analysis by western blot - (reply: 2)
     
534. Preparing supernatant from cell culture for western blot - (reply: 4)
     
535. Fast/easy way to measure cell size - (reply: 4)
     
536. Which is the best way to generate growth curves for cancer cell lines - (reply: 7)
     
537. Cell number issue - Nile Red staining - (reply: 3)
     
538. Music Video on Cell Division/Mitosis - (reply: 1)
     
539. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
     
540. Integrin B1 Staining in HT1080 cells - (reply: 1)
     
541. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
     
542. My cells won't pellet - any ideas? - (reply: 6)
     
543. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
     
544. EMSA: cell extract vs purified protein - (reply: 2)
     
545. Insect cells transfection reagent - (reply: 1)
     
546. Low Protein Content In Cell Lysate - (reply: 1)
     
547. Can you see GFP after lysis mammalian cells? - (reply: 5)
     
548. Centrifugation speeds for cells. - (reply: 8)
     
549. Cell recovery from ethanol fixation - (reply: 4)
     
550. Cryopreservation of MSC at lower cell density - (reply: 2)
     
551. C-terminal GST fusion problem in mammalian cell - (reply: 6)
     
552. Identify cell culture contamination - (reply: 2)
     
553. Adipose stem cell culture - (reply: 5)
     
554. Determine cell proliferation by viability assay ? - (reply: 6)
     
555. Apoptosis via cell to cell contact - (reply: 1)
     
556. Cells stop to grow - (reply: 6)
     
557. Pore formation in cells after adding amphotericin B in culture medium - (reply: 2)
     
558. Long Treatment, Rapidly Dividing Cells - (reply: 7)
     
559. Need help calculating cell number to plate - (reply: 1)
     
560. Injection of human cell culture conditioned media into nude mice - (reply: 1)
     
561. How long can I store cell lysis buffer at 4C for? - (reply: 1)
     
562. Heeeeeelp..cell cycle sample..is it possible to store it? - (reply: 2)
     
563. Subculture issue of primary cell - (reply: 6)
     
564. Differentiating mouse from human cells with PCR - (reply: 5)
     
565. primary valve interstitial cells transfection - (reply: 1)
     
566. Stem Cell Donation - (reply: 7)
     
567. isolating DNA from cells in suspension - (reply: 5)
     
568. Software for designing cell pathways - (reply: 1)
     
569. Tamoxifen citrate solubility for cell work?? - (reply: 1)
     
570. Centrifuged cells stick to wall of reaction tube - (reply: 12)
     
571. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
     
572. Changing Genomic Regions in Host Cell - (reply: 1)
     
573. How to transform adherent cells to suspension (CHO cells) - (reply: 2)
     
574. my competent cell grow on any agar plate - (reply: 7)
     
575. Bacterial Contamination in HuEmbryonic cell lines seen in SFM4 but not in DMEM + - (reply: 3)
     
576. Cell attachement occuring after 48 hours, not 24 hours. - (reply: 3)
     
577. Transfection of a non human gene into human cell line - (reply: 1)
     
578. Procedures to replace vial of cells and passage number. - (reply: 4)
     
579. Immunofluorescence on cells - can I keep the coverslips unstained in PFA? - (reply: 4)
     
580. p14ARF and MDM2 in transformed cells - (reply: 1)
     
581. RNA isolation with low number of cells (1*10^4 to 5*10^4) for RT-PCR - (reply: 3)
     
582. contamination of human cell lines with yeast - (reply: 3)
     
583. Generation of single cell suspensions from neural tissue - (reply: 2)
     
584. Is this contamination or cell death? - (reply: 7)
     
585. BaF3 cell culture in 96 well plates - (reply: 2)
     
586. THP-1 differentiated cells detach when washing with PBS (Mg and Ca free PBS) - (reply: 2)
     
587. unwanted cell lysis during LB+Amp incubation - (reply: 5)
     
588. Cells spontaneously detaching? - (reply: 1)
     
589. Immuno-staining for cell surface/membrane markers after permeabilisation - (reply: 4)
     
590. different type of media for cell culture - (reply: 1)
     
591. Influenza: cell and mechanism information? - (reply: 1)
     
592. Can CMV promoter start transcription in prokaryote cells - (reply: 1)
     
593. How to treat cells with TNFa - (reply: 2)
     
594. CLOUDINESS of RIN5F cell culture - (reply: 7)
     
595. U87MG cells not growing well - (reply: 3)
     
596. Concentrating low volume of cells - (reply: 8)
     
597. Preparing a conditioned medium from cancer cells - (reply: 3)
     
598. Presence of melanin in melanin containing cell line - (reply: 1)
     
599. Cancer cells culture - (reply: 1)
     
600. How to achieve uniform cell distribution in culture vessle - (reply: 6)
     
601. viability/integrity stain of fungal cells - (reply: 5)
     
602. Cell culture contamination - (reply: 3)
     
603. airway primary cells stop growing at passage 3 - (reply: 1)
     
604. cell culture neural cells easier? - (reply: 6)
     
605. Question - Corticosterone, cell culture - (reply: 1)
     
606. pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 30)
     
607. Where to obtain rat embryonic stem cells? - (reply: 3)
     
608. Stable cell line - (reply: 1)
     
609. PBMC cells handling - (reply: 1)
     
610. myc-tag antibody makes multiple bands, even in mock & parental cells - (reply: 1)
     
611. Unable to clone in E. coli cells - (reply: 3)
     
612. removing MHC class I from the cell surface - (reply: 3)
     
613. Osteosarcoma cell culture problem - (reply: 7)
     
614. rtPCR stem cell signalling array from quiagen- has anybody experience? - (reply: 1)
     
615. Thawed cells spent 4 hours extra in DMSO.. problem? - (reply: 4)
     
616. Thawed cells spent 4 hours extra in DMSO.. problem? - (reply: 1)
     
617. colon cancer cell lines vs normal colon epithelial cells - (reply: 1)
     
618. Cell count and seed, plz help... - (reply: 4)
     
619. In and out of cell Help 2 - (reply: 6)
     
620. In and out of cell Help - (reply: 2)
     
621. Primary culture of breast tumor cells - (reply: 1)
     
622. Strange Cell Morphology & Cell Debris Following Lentiviral Transduction - (reply: 2)
     
623. Cell numbers versus confluency - (reply: 2)
     
624. Flow cytometry in THP-1 cells - (reply: 1)
     
625. Competent bacterial cells - (reply: 2)
     
626. Could a change of FBS affect outcome of a cell proliferation assay? - (reply: 4)
     
627. How do they make passage limited cells? - (reply: 1)
     
628. transient transfection problem using ME180 cells - (reply: 1)
     
629. How to completely remove cells from surface - (reply: 3)
     
630. Hiperfect siRNA in HT-29 cells help - (reply: 2)
     
631. Isolation of BOTH cells AND serums from mouse blood - (reply: 5)
     
632. Transfection of pCI-neo plasmid into 3T3-L1 cells - (reply: 1)
     
633. Commercial source Stomach Smooth Muscle Cell lysate - (reply: 1)
     
634. DMEM or EMEM Does it matter? (HeLa Cells & Transfection) - (reply: 3)
     
635. cell line contaminated by something... - (reply: 2)
     
636. Flag Staining in U87 cells - (reply: 4)
     
637. protein is not expessing in HEK 293 cells - (reply: 2)
     
638. competent cells: CaCl2 autoclave vs filter sterilisation - (reply: 12)
     
639. HepG3 cell line origin - (reply: 3)
     
640. dilution of cell suspension - (reply: 4)
     
641. how to preserve spleen cells for long duration, for flowcytometry - (reply: 2)
     
642. DNA extraction for MCF-7 and HeLa - (reply: 4)
     
643. Increasing cell growth rate in plain M9 medium - (reply: 1)
     
644. Puromycin - shRNA - stable cell line for knock down - (reply: 4)
     
645. Reducing cell adhesion - (reply: 1)
     
646. The Hek 293T cell line looks like non adherent cells after to be sorted in FACS - (reply: 1)
     
647. Hygromycin selection of virally-transduced cell lines - (reply: 1)
     
648. Which cells express which genes? - (reply: 1)
     
649. IGF1 stimulation of THP1 cells - (reply: 2)
     
650. Cells detach during bodipy 493/503 staining - (reply: 1)
     
651. RNA isolation from small numbers of cells - (reply: 6)
     
652. Distinguish murine and human cells by surface staining? - (reply: 1)
     
653. starting HaCaT cell line. basic questions - (reply: 6)
     
654. culture media for pancreatic cancer stem cells - (reply: 2)
     
655. Lysis method for single human embryonic cell - (reply: 5)
     
656. In vitro transcription/translated protein to cells!!! - (reply: 1)
     
657. Cell plating - (reply: 2)
     
658. sample preparation from whole cell lysates for Edman degradation - (reply: 2)
     
659. colony formation assay in A2780 cells - (reply: 4)
     
660. Cell confluency, transfection, protein expression for 293T cells - (reply: 2)
     
661. Which is a better way to culture cells after thawing ? - (reply: 2)
     
662. Comet assay sperm cells - (reply: 5)
     
663. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
     
664. Live/Dead Staining of already Permeable Cells - (reply: 10)
     
665. cell synchronisation for fibroblasts (help choosing method/chemical) - (reply: 2)
     
666. Cell Therapies - (reply: 2)
     
667. SURE E. coli competent cells - (reply: 1)
     
668. Stable protein expression in Vero cells - (reply: 2)
     
669. HL60 cell line suppliers - (reply: 1)
     
670. Insoluble material floats over my SDS lysate of human cells after centrifugation - (reply: 7)
     
671. Cell Fixation for DNA flow cytometry in fibroblasts - (reply: 2)
     
672. Question about HeLa cell overconfluence - (reply: 3)
     
673. 293T cell contamination post-transfection - (reply: 1)
     
674. HEK293T cells are too fragile?? - (reply: 2)
     
675. number of splitting after defrozing cells - (reply: 2)
     
676. my human iPS cells have a lot of differentiation - (reply: 4)
     
677. Properly designing a drug treatment assay measuring cell cycle arrest - (reply: 2)
     
678. Detaching Breast Cancer cells (MCF-10A, HCC70) without trypsin- used for FACs - (reply: 4)
     
679. counting cells before adding them to 96 well plate - (reply: 7)
     
680. Cell Suspension - (reply: 2)
     
681. What will happen if the cell culture medium is suddenly changed to another one? - (reply: 5)
     
682. MDA-MB-231 cells undergoing senescence- help! - (reply: 2)
     
683. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
     
684. coating cover-slides before seeding cells for fluorescent microscopy - (reply: 4)
     
685. How to induce early apoptosis in cells without using chemicals or UV - (reply: 5)
     
686. choice of cell line for orthotopic xenograft implant - (reply: 3)
     
687. Compound concentration depending on cell number? - (reply: 4)
     
688. How to remove mycoplasma contamination in cell lines? - (reply: 5)
     
689. [Question] How to remove cell debris stuck on the attached cells in primary card - (reply: 2)
     
690. gene overexpression in cells: virus transduction or transfection following selec - (reply: 4)
     
691. Reagent used to adjust pH of cell culture media - (reply: 3)
     
692. Powder indomethacin in cell culture media?? - (reply: 2)
     
693. How many generation of HEK293 cell can undergo? - (reply: 1)
     
694. [Question] how to make primary-cultured cells evenly distributed? - (reply: 11)
     
695. small dots in cell culture - (reply: 2)
     
696. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
     
697. Clumpy competent cells - (reply: 3)
     
698. Preparation of bacterial cells for SEM and TEM - (reply: 2)
     
699. G418 advice in estab;lishing stable cell line - (reply: 1)
     
700. Electroporation of J774.2 murine macrophage cell line - (reply: 1)
     
701. About trypsinizing adherent cells in 96-well plates - (reply: 2)
     
702. Passaging cells - (reply: 12)
     
703. Preparing homemade XL1-blue cells in CCMB80 buffer - (reply: 1)
     
704. Understanding Cell Passaging - (reply: 5)
     
705. How to extract DNA from small number of cells - (reply: 2)
     
706. How to identify beta pancreatic cells in dish of heterogenous cells? - (reply: 12)
     
707. Cell Splitting Ratio Question - (reply: 5)
     
708. Sf9 cell suspension culture - (reply: 5)
     
709. competent cell formation - (reply: 1)
     
710. Help! Clontech Xfect is killing my T-Rex HeLa cells!!! Why! - (reply: 1)
     
711. How long are cells viable on ice - (reply: 2)
     
712. Discrepancy between tissue and cell culture - (reply: 1)
     
713. What is the density of a human skin cell? - (reply: 8)
     
714. Determining the % of GFP/CHERRY cells in co-mixed culture - (reply: 3)
     
715. Is the genomic DNA similar among different cell lines? - (reply: 4)
     
716. serumm free culture of adherent cho cells - (reply: 1)
     
717. detection of mesenchymal stem cells in cancer tissue sample - (reply: 1)
     
718. Can u suggest any myoepithelial cell isolation protocol with the help of reagent - (reply: 1)
     
719. cell line cultured in suspension - (reply: 1)
     
720. GFP cells in paraffin.....orange? - (reply: 1)
     
721. overexpression using pcDNA3.0 in stably knockdown cells - (reply: 3)
     
722. Cell staining for fluorometric assay - (reply: 5)
     
723. Would E. Coli cells be stable at room temp, if in media with glycerol? - (reply: 1)
     
724. Long-Term Culturing of SH-SY5Y Cells - (reply: 1)
     
725. N acetyl cysteine solution for cell culture - (reply: 3)
     
726. Aortic smooth muscle cell contamination - (reply: 1)
     
727. need recommendation for stable transduction of fibroblast cells - (reply: 1)
     
728. Sub G1 in cell cycle - (reply: 2)
     
729. DMSO induces cluster growing in LNCaP cells? - (reply: 1)
     
730. Cell cycle arrest - adherent cells going into suspension - (reply: 4)
     
731. Prevent adherent cells from adhering - (reply: 3)
     
732. INS-1E cells not adhere to the flask! - (reply: 1)
     
733. A549 cells are not attaching to flask - (reply: 2)
     
734. irradiated J774 cells - (reply: 1)
     
735. Cell lysis with proteinase k - (reply: 2)
     
736. One siRNA sequence, many cell lines - (reply: 1)
     
737. Freezing cell lysates for westerns - (reply: 2)
     
738. SDS-PAGE for cell culture medium - sample preparation - (reply: 1)
     
739. adherence to host cell question - (reply: 2)
     
740. New patch of sf9 cells not growing - (reply: 2)
     
741. contamination in yeast cell culture - (reply: 8)
     
742. Tissue culture contamination like bacteria inside the cells. What is this kind o - (reply: 1)
     
743. sf9 Insect cells questions - (reply: 3)
     
744. Kit to mesure cell death - (reply: 4)
     
745. An improve method for making Competent Cells without heat shock. How? - (reply: 2)
     
746. J774 and Flow cytometry....are they really big cells ? - (reply: 9)
     
747. immunostaining cells grown in matrigel - (reply: 1)
     
748. Cell concentration for flow cytometry - (reply: 2)
     
749. Transient Transfection: Adherent vs. Suspended/Floating Cells - (reply: 7)
     
750. Cells growing very slowly - (reply: 6)
     
751. SH-SY5Y cells do not grow - (reply: 1)
     
752. GH3 cells - (reply: 4)
     
753. The connection between LPS and PMA stimulation for maturation of ThP1 cells - (reply: 2)
     
754. Using normal competent cells in electroporation method? - (reply: 1)
     
755. Washing n2a cells out of 75 cm flask? - (reply: 1)
     
756. How to dissociate N2A cells after centrifugation? - (reply: 3)
     
757. Undefinable particles in cell culture flask - (reply: 2)
     
758. Help: transduction of primary blood cells (T): lentiviral vs. adenoviral vector - (reply: 1)
     
759. immunostaining of cells in culture - (reply: 1)
     
760. healthy stem cells vs cancer stem cells - (reply: 1)
     
761. values for mRNA molecules per cell - (reply: 1)
     
762. Help!! need to culture PC3M cell line. Any advice? - (reply: 5)
     
763. MCF10A Tet-ON stable cell line generation - (reply: 1)
     
764. Primary cell line - (reply: 2)
     
765. Neurosphere Assay with neuroblastoma cell lines - (reply: 1)
     
766. How to decontaminate adherent cells with gentamicin - (reply: 2)
     
767. Contamination in CHO cell ? - (reply: 3)
     
768. Thawing Hek 233t cells- but cells dying!! - (reply: 3)
     
769. How to purify RNA from a handful number of cells? - (reply: 5)
     
770. Check cell viablity after FACS - (reply: 2)
     
771. ISH on fluorescing cells - (reply: 1)
     
772. Please help, may i replenish Glutamine in cell culture medium? - (reply: 3)
     
773. Protein extraction from dead cells - (reply: 1)
     
774. Cells in suspension vs. plated - (reply: 2)
     
775. PI stain for cell viability~~Compensation? Suggestion needed~~^^ - (reply: 5)
     
776. Hepg2 cells growing on top of each other - (reply: 1)
     
777. urgent! calculating concentration of compound with different cell amounts - (reply: 3)
     
778. h9c2 cells culture - (reply: 5)
     
779. Does anyone have experience of subjecting cultured cells to mechanical stress? - (reply: 1)
     
780. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
     
781. cell population doubling - (reply: 1)
     
782. Seeding HeLa cells into 96 well plate for transfection - (reply: 2)
     
783. Cell line licence - (reply: 1)
     
784. adenoviral cre or lentiviral cre for mouse primary cells? - (reply: 2)
     
785. temperature tolerance of animal cells - (reply: 3)
     
786. only one plasmid expressed highly after co-tranfection into cells - (reply: 1)
     
787. pERK and pAKT in MDA-MB-231 cells by western blot! - (reply: 1)
     
788. Human insulin for cell culture? - (reply: 2)
     
789. violet laser (405nm) and cell cycle staining with DAPI - (reply: 1)
     
790. Competent cells question: JM101 vs DH5alpha - (reply: 2)
     
791. Trouble producing stable cell lines - (reply: 2)
     
792. Infecting cells with virus - (reply: 1)
     
793. Requesting basic protocols on cell sorting - (reply: 1)
     
794. lysing mammalian cells in sds - (reply: 3)
     
795. Is it possible to extract RNA from Cells frozen in 10% DMSO ? - (reply: 2)
     
796. Is it possible to assay the carbohydrate profile of a cell? - (reply: 2)
     
797. changing cell culture media - (reply: 1)
     
798. Preparing Palmitate:BSA solution for treatment of cells? - (reply: 7)
     
799. trypsinization of adherent cells - (reply: 9)
     
800. Mitochondria pelleted together with nuclei from 293 Cells using Sucrose gradient - (reply: 3)
     
801. Aberrant (apparently) nuclear immunostaining for VEGF in Glial cells - (reply: 2)
     
802. How to lysis cells for an RNA extraction - (reply: 1)
     
803. Problem with THP-1 cell line - (reply: 3)
     
804. normalization to protein content or cell number - (reply: 2)
     
805. detection of phosphorylation from cell pellet - (reply: 2)
     
806. are THP-1 Cells(Monocytes) or Macrophages resistant to Mycoplasma infection or - (reply: 4)
     
807. Recommended competent cell for transformation of large plasmids? - (reply: 4)
     
808. Cell culture shared with yeast? - (reply: 2)
     
809. E. coli cells refuse to grow! - (reply: 2)
     
810. how to fraction stem cell at different cell cycle stage - (reply: 2)
     
811. Making Nuclei From Mouse Cells - (reply: 1)
     
812. Different cell lines different protein stability - (reply: 1)
     
813. Neutrophil cell lines and plasma cell lines of choice - (reply: 1)
     
814. Immunofluorescence Staining of Cells - (reply: 1)
     
815. Nucleus fills the whole cell - (reply: 3)
     
816. Generating stable cell line with linear DNA - (reply: 3)
     
817. isolating cells from Cockroach - (reply: 2)
     
818. Question about suspension cells - (reply: 7)
     
819. Calu-3 cell line do not grow properly - (reply: 1)
     
820. Detach cells and stop stimulation simultaneously- How? - (reply: 4)
     
821. Counting and plating cells - (reply: 3)
     
822. cells die 4-6 days after thawing - (reply: 8)
     
823. Cell Envelope Proteins Smeared - (reply: 1)
     
824. Calculating the amount of the desired cells in the medium ? - (reply: 3)
     
825. long term storage of cells at -80 - (reply: 1)
     
826. Making BL21 competent cells - (reply: 2)
     
827. cell plating density - (reply: 2)
     
828. What kind of cell culture contamination is it? - (reply: 8)
     
829. Problem with preparing unicellular suspension from MCF7 cells - (reply: 3)
     
830. xenograft cells harvested from spleen of SCID/NOD mice - (reply: 1)
     
831. to treat cells with inhibitor - (reply: 2)
     
832. lysis buffer not lysing adherent cells? - (reply: 8)
     
833. Culturing cells on a slide - (reply: 5)
     
834. Culturing cells on a slide - (reply: 3)
     
835. protocol for immunostaining primery cells - (reply: 5)
     
836. How to wipe out fragment of dead cells from the suspension cells? - (reply: 1)
     
837. How to Dilute Cell Lysate for Luciferase Assay - (reply: 2)
     
838. What's the function of cholera toxin in cell culture medium? eg. MCF10A cell - (reply: 2)
     
839. Could the use of different FBS affact my cells - (reply: 5)
     
840. Analysis of high throughput screen for cell surface markers - (reply: 1)
     
841. Lymphoblastoid cell culture - (reply: 9)
     
842. SF9 Cells lose exponential growth, lack stationary phase - (reply: 3)
     
843. Don't know why my cells are unhealthy - (reply: 2)
     
844. Hybridoma cells not secreting antibody - (reply: 3)
     
845. Determining gene of interest from Cell culture lines - (reply: 2)
     
846. Three clones of a cell line? - (reply: 3)
     
847. Freezing cells in DMSO straight into minus 80? - (reply: 4)
     
848. MCF7 cells vitality - (reply: 3)
     
849. Ultrasonic bath for cell lysis - (reply: 3)
     
850. any methods to evaluate the attaching ability of suspension cells? - (reply: 6)
     
851. Vascular Smooth Muscle cell Marker - (reply: 3)
     
852. Foam in Cell Culture - (reply: 1)
     
853. Where can I find more info on how certain ionic solutions can affect cell biolog - (reply: 4)
     
854. SURE cells - (reply: 1)
     
855. DT40 Cell Synchronisation - (reply: 2)
     
856. Bio-Rad Cell counter - (reply: 3)
     
857. SKBR-3 cells dying - (reply: 3)
     
858. New cell culture user with no teacher or trainer - (reply: 5)
     
859. Help with some problems with Culturing Epithelial Cells - (reply: 5)
     
860. NIH3T3 cells are suddenly dying a few days after thawing and expanding - (reply: 1)
     
861. Stable cells survive with antibiotics but no exogenous expression detected with - (reply: 2)
     
862. What are these large multinucleated cells??? - (reply: 1)
     
863. Searching for a live cell staining method - (reply: 1)
     
864. WT BRAF/NRAS mouse Melanoma cells - (reply: 2)
     
865. HEK 293 cells Detachement - (reply: 8)
     
866. Cell culture in candle jar - (reply: 2)
     
867. centrifugation speed/cell prep for flow - (reply: 4)
     
868. Huge background with HT22 cells phophoERK antibody - (reply: 3)
     
869. Huge background with HT22 cells phophoERK antibody - (reply: 1)
     
870. Competency cells analysis - (reply: 1)
     
871. How many cells needed for co-IP and mass spec - (reply: 4)
     
872. Performing MTT assay in suspension cells (new in the subject) - (reply: 8)
     
873. cells die after transfection - (reply: 1)
     
874. Seeding cells on small metal screws - (reply: 1)
     
875. Cell lysate turbidity upon long storage - (reply: 3)
     
876. Host cells for transformation - (reply: 3)
     
877. Splitting or feeding cells for the weekend - (reply: 1)
     
878. Black moving particles in the cell Culture - (reply: 2)
     
879. cell culture - (reply: 1)
     
880. cells shrink, detach from each other when changing media - (reply: 3)
     
881. Newbie help: Seeding cells to 6-well plates - (reply: 2)
     
882. 3t3 cell tured black - (reply: 9)
     
883. calculating the number of cells to freeze down - (reply: 2)
     
884. Fixed cells, sticky cells? - (reply: 2)
     
885. What is cotransfection in molecular cell biology? - (reply: 1)
     
886. serum deprivation of cell lines - (reply: 1)
     
887. serum deprivation of cell lines - (reply: 1)
     
888. What to do Post-transfection of cells with siRNA? - (reply: 2)
     
889. Problem with HEK stable cell line - (reply: 1)
     
890. HEK 293 cell culture - (reply: 3)
     
891. HepG2 cells resistant to Trypsinization - Please help!! - (reply: 8)
     
892. difficulty in passaging HK-2 cells - (reply: 5)
     
893. difficulty in passaging HK-2 cells - (reply: 2)
     
894. qPCR in primary cells - housekeeping CT-values sample-specific bad - (reply: 1)
     
895. Expression of protein X varies in different w.b running (in one cell line, but - (reply: 3)
     
896. More cells in an antibiotic plate than in a plate without antibiotic???? - (reply: 2)
     
897. what are these cells?? - (reply: 1)
     
898. How to choose the right plasmids for certain types of cell? - (reply: 1)
     
899. Isolation of rat endothelial cells - (reply: 2)
     
900. protocol for making e.coli competent cells under low temperature - (reply: 1)
     
901. Looking for cell surface markers. - (reply: 3)
     
902. common nuclear marker for T cells - (reply: 1)
     
903. How is the cell protected from the hydrolase enzymes of the lysosomes? - (reply: 1)
     
904. Any reliable markers for mouse memoery T cells? - (reply: 1)
     
905. Do mouse endothelial cells express the alphaVbeta3 integrin? - (reply: 1)
     
906. Cell Culture debris or contamination? - (reply: 4)
     
907. primary cell isolation from CNS, how to remove myeline debris - (reply: 1)
     
908. Platinum-A cell trasnfection toruble - (reply: 1)
     
909. cell culture media refrigeration - (reply: 4)
     
910. abnormal epithelial cells in mouse mammary gland - (reply: 1)
     
911. cell DNA content of cells grown on different scaffolds - (reply: 2)
     
912. Cancer cell starvation - (reply: 1)
     
913. Why are HEK-293 cells difficult to perform clonal selction? - (reply: 4)
     
914. Housekeeping gene for COS-1 cells - (reply: 1)
     
915. applying cell culture protocol of protein extraction for rat intact tissue is co - (reply: 1)
     
916. Monocytes die after cell scraping - (reply: 2)
     
917. The water pan got dry in the TC incubator. Will my cells be OK? - (reply: 1)
     
918. Cell fractionation - (reply: 2)
     
919. Media formulations for MDA-MB-231, BT-474 cells and Caco-2 cells - (reply: 1)
     
920. Proper storage of isolated white blood cell prior to RNA isolation - (reply: 3)
     
921. Dropped cells into liquid nitrogen tank - (reply: 3)
     
922. 5-FOA media not letting ANY cells grow... help! - (reply: 1)
     
923. Small intestinal cell line for transport studies - (reply: 4)
     
924. help needed in determining volume of cells to be seeded - (reply: 6)
     
925. Dilution, Cell concentration & DNA content - (reply: 3)
     
926. competent cells - (reply: 2)
     
927. Cell lines & gene of interest - (reply: 1)
     
928. Software for selection genes related to concrete cell processes - (reply: 1)
     
929. Immunocytochemistry with HEK293 cells - (reply: 1)
     
930. How to determine apoptotic cell types in-vivo? - (reply: 1)
     
931. Isolating Genomic DNA from a small colony of cultured human cells - (reply: 1)
     
932. data analysis for single cell size and distribution - (reply: 1)
     
933. determining optimal cell count for MTT - (reply: 2)
     
934. Extracting DNA from FACS sorted cells - (reply: 3)
     
935. Storing frozen cells in -80C instead of liquid nitrogen - (reply: 7)
     
936. phosphate + freeze thaw vs RIPA buffer for cell lysis - (reply: 2)
     
937. DG44 CHO cells and Methotrexate - (reply: 1)
     
938. extract plasmids from transfected eukaryotic cells - (reply: 6)
     
939. Staining Cells in Suspension - (reply: 4)
     
940. cell culture confusion - (reply: 1)
     
941. a gene expressed in 293 cell lines but not Hela cells - (reply: 1)
     
942. chambered cell culture-slide for confocal microscopy - (reply: 3)
     
943. Nicotiana tabacum (BY-2) Suspension cells contaminated! Help! - (reply: 1)
     
944. Dead or Alive... -_- (MCF-7 cells) - (reply: 7)
     
945. how to wash cells after fixation for a live binding study - (reply: 1)
     
946. Evenly distribute 100ul of cells in a 48/96 well - (reply: 2)
     
947. Cell lysis before shearing - (reply: 5)
     
948. really low puromycin concentration for selection of cells - (reply: 6)
     
949. Single stain control for 293 cell line - (reply: 4)
     
950. loading dendritic cell with antigen - (reply: 1)
     
951. Cell Lysis and Western Blotting - (reply: 1)
     
952. problem with embryo carcinoma cell line , p19 , - (reply: 1)
     
953. In vitro induction of apoptosis in tumor cells by inactivated NDV and IAV - (reply: 1)
     
954. problem with embryo carcinoma cell line , p19 , - (reply: 1)
     
955. Recombinant protein from cell lysate - (reply: 4)
     
956. How to test if a compound is entering cells? - (reply: 3)
     
957. help with cell seeding - (reply: 2)
     
958. SURE or Stbl competent cells for retroviral vector? - (reply: 2)
     
959. RNA isolation from frozen cells - (reply: 2)
     
960. How do you coat coverslips before seeding cells? - (reply: 3)
     
961. Buy.Ask for buying some cell lines and antibodies - (reply: 3)
     
962. competentce cell, cloning - (reply: 2)
     
963. Flp-recombinase target site integration in 293 T-REx cells - (reply: 1)
     
964. Storage of transfected PC12 cells - (reply: 1)
     
965. cell adhension - (reply: 1)
     
966. Using Methanol For Cell Lysis - (reply: 1)
     
967. Adjusting pH of cell culture medium for ELISA - (reply: 1)
     
968. Help finding siRNA targets in MEF and HEK293 cells needed - (reply: 4)
     
969. Help needed with stable cell line creation - problems making a cell line expressing a viral protein (reply: 3)
     
970. Contamination of primary cells with fibroblast. - (reply: 1)
     
971. Help With Lysing HEK293 & Fibroblast Cells - (reply: 1)
     
972. problem with siRNA transfection of HEK cells - (reply: 5)
     
973. Splitting A549 cells - (reply: 9)
     
974. NMDA Transfection in 293 Cells - (reply: 1)
     
975. freezing adherant cells - (reply: 2)
     
976. freezing adherant cells - (reply: 2)
     
977. Total cell lysate from T cells - hard to lyze all the nuclei with RIPA (reply: 2)
     
978. How the heck does serum activate Akt?!? - Is serum starvation necessary in endothelial cells? (reply: 1)
     
979. cells stably expressing T7 RNA polymerase - (reply: 1)
     
980. Cell Fixation - Experience with cell fixation? (reply: 5)
     
981. picking up colonies and growing in the broth after transformaion - cloning with competent cells (reply: 1)
     
982. Is pCL-Eco suitable for packaging viruses that used to infect human cells? - (reply: 1)
     
983. Cause of Death for Cell Culture? - (reply: 5)
     
984. creat a SY5Y stable cell line - (reply: 1)
     
985. selecting drug-resistant colon cancer cells - (reply: 2)
     
986. Help, problems with expressing human proteins in E.coli - IPTG induced cells turn pink (reply: 1)
     
987. Helper T cell skewing - (reply: 3)
     
988. Optimum serum starvation period for NIH, CHO, COS7 cells - (reply: 4)
     
989. Help me brainstorm an explanation for this cell culture contamination - Cell Culture, contamination, NIH 3t3 (reply: 7)
     
990. competent cells stock - (reply: 3)
     
991. intracellular cytokine staining - re-stimulation of activated T cells (reply: 1)
     
992. DH5alpha cells - (reply: 4)
     
993. pheonix cells and &293A cells - (reply: 1)
     
994. could freezing/thawing make mammalian cells competent - could it change phenotype back? (reply: 6)
     
995. Protein purification from HEK 293 cells - (reply: 1)
     
996. Growing hybridoma cells in medium without serum - (reply: 2)
     
997. Cells sensitive after replacing media - what am I doing wrong? - (reply: 4)
     
998. Cell drawing tools? - (reply: 6)
     
999. How many cells in plant embryos ? - seed biology (reply: 1)
     
1000. cell count - (reply: 8)
      
1001. question on making chemical and electrocompetent cells - (reply: 2)
      
1002. COS7 cells giving me problems - (reply: 1)
      
1003. Where to get 5T series cells? - (reply: 1)
      
1004. cell number for colony formation - HEK293, NIH3T3 (reply: 1)
      
1005. Electroporation/electrotransfection mammalian cells - (reply: 7)
      
1006. Cell debris here, there, everywhere!? - (reply: 2)
      
1007. Cell debris here, there, everywhere!? - (reply: 1)
      
1008. Mini prep of frozen bacteria cells - (reply: 2)
      
1009. Help! Turned on UV light. Did I kill my cells? - (reply: 2)
      
1010. growing competent cells - (reply: 1)
      
1011. Freezing density for NIH 3T3 cells - (reply: 3)
      
1012. Stable Cell Line generation - (reply: 3)
      
1013. making more competent cells - (reply: 2)
      
1014. CellTracker dye in necrotic cells? - (reply: 1)
      
1015. The case of the punky Sf9 cells - (reply: 2)
      
1016. sticky perm cells (tween 20)- can I prevent it? - (reply: 2)
      
1017. Preparation of mammalian cell lysis - Preparation of mammalian cell lysis for Western blotting (reply: 7)
      
1018. collecting cells HT1080 - (reply: 4)
      
1019. Expired media - How long after the expiration data is cell culture media good? (reply: 2)
      
1020. Help: problem with MDCK cells - (reply: 2)
      
1021. bacterial Cell free culture supernatants - Can any one help me out to prepare a cell free culture supernatant (reply: 3)
      
1022. Oh my cell culture problems never cease!!! - (reply: 4)
      
1023. WEHI-3BD cell culturing - Advice on culturing. (reply: 5)
      
1024. cell homogenization - (reply: 13)
      
1025. cell count - (reply: 3)
      
1026. cell lysis - confocal slides (reply: 4)
      
1027. HeLa cell anatomy question - (reply: 3)
      
1028. Need cell lines wich express separately TLR 2, 4 and 5 - (reply: 1)
      
1029. MSC in culture - stop growing? - Mesenchymal stem cells in culture randomly stop expanding (reply: 2)
      
1030. THP-1 cell line - (reply: 3)
      
1031. CaMKII alpha promoter and cell tranfection - (reply: 1)
      
1032. Adaptation of mammalian cells to serum free mode - (reply: 2)
      
1033. Live cell Imaging medium - (reply: 1)
      
1034. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
      
1035. MDA-MB-231 cell transfection - (reply: 5)
      
1036. Looking for protocol to do biotinlynation on each single protein in cell lysate - (reply: 1)
      
1037. Break Cell Clumps - prepare single cells for injections (reply: 1)
      
1038. Stem Cells Sphere - stem cells (reply: 2)
      
1039. Request for cell lines? - (reply: 1)
      
1040. Is 2% pen strep too much for cell culture? - will my cells die a painful death? (reply: 3)
      
1041. MTT problems with Huh7 cell line, plzzzzzzz help - MTT color problem (reply: 5)
      
1042. Calculating cell doubling time - (reply: 1)
      
1043. SPONTAEOUS DEATH OF CELLS in culture - (reply: 8)
      
1044. Infected cells that are dying after puromycin selection - (reply: 1)
      
1045. contamination issue in cell culture - (reply: 1)
      
1046. Why do cells need to be washed in PBS before biotinylation? - (reply: 3)
      
1047. AAD positive means late apoptosis? - About cells that are simultaneously positive for annexin V and AAD (reply: 2)
      
1048. Purification of recombinant proteins from insect cells - (reply: 2)
      
1049. Cell cycle evaluation in cells with more than one nuclei - (reply: 8)
      
1050. Help! Transfection newbie with a dumb question - Trying to transfect adherent cells (reply: 1)
      
1051. Tissue vs cell line lysate - Improve sample quality? - (reply: 4)
      
1052. Growth curve for PC3 cell line - (reply: 1)
      
1053. Agrobacterium EHA101 competent cells - (reply: 1)
      
1054. THP-1 cell line - (reply: 2)
      
1055. Effective DCFDA Protocol for Non-Adherent THP-1 Cells? - (reply: 1)
      
1056. Stripping Buffers that won't kill my cells? - Recipe for 0.1 M Tris-Acetate 2.0 M NaCl pH 7.7 (reply: 3)
      
1057. best freezing method for electrocompetent cells - (reply: 9)
      
1058. cell seeding calculation - (reply: 8)
      
1059. Improved Stem Cell Proliferation - Would interested in improving proliferation of adult stem cells? (reply: 5)
      
1060. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
      
1061. Yeast contamination in cell culture - (reply: 8)
      
1062. Problems with Scrambled Control - Scrambled controls are inhibiting cell growth more than test siRNA (reply: 2)
      
1063. Remove cells from dish and fix them in slides - (reply: 3)
      
1064. Help- lysing cells with triton x-100 - (reply: 1)
      
1065. E.coli cells lyse in minimal media - (reply: 3)
      
1066. How stable are suspension cells in room temperature? - (reply: 2)
      
1067. Help with HELA and HEK293 cell seeding for transfection - (reply: 4)
      
1068. Transfection in insect cell lines?? - (reply: 1)
      
1069. PCR genomic DNA using cell lysate - (reply: 1)
      
1070. HELA Cells Capable of producing whole, infective HPV particles? - (reply: 3)
      
1071. Distilled water for Electrocompetent Cell Preparation - (reply: 6)
      
1072. Should I fix cells digested from tissues? - (reply: 4)
      
1073. STR cell line identification - looking for cell line identification method (reply: 1)
      
1074. Establishing Sub-cutaneous Xenograft Cancer Model - How many millions of cells to inject for subcutaneous models (reply: 3)
      
1075. Rat Dental Pulp Stem Cell Culture - (reply: 1)
      
1076. NK cell analysis using CD16PE and CD56PE - (reply: 2)
      
1077. My neuroblastoma are floating!! please help - Both SK-NSH and SH-SY5Y are adherent cells, but are only floating now (reply: 3)
      
1078. cell suspencion and medium - (reply: 5)
      
1079. One Shot TOP10 Cells - (reply: 6)
      
1080. Help on BmN4 cell culture - How to maintain BmN4 cells? (reply: 1)
      
1081. Compare ChIP to cell lysate - (reply: 1)
      
1082. Compare ChIP to cell lysate - (reply: 1)
      
1083. Testing cytoxixity on healthy human cells - (reply: 2)
      
1084. what cell line to use - to demonstrate ubiquitination (reply: 1)
      
1085. GFP cells intracellular staining - (reply: 1)
      
1086. Xenograph - how to maintain cell viability? - (reply: 1)
      
1087. Western blot of apoptotic cells - (reply: 2)
      
1088. cell sycle and proliferation - (reply: 3)
      
1089. help with cell dilution - (reply: 2)
      
1090. Difficult Cell Proliferation Experiment - (reply: 2)
      
1091. Cell dilution cloning - Cells cloning (reply: 2)
      
1092. Which cells to use ? - Chang liver or normal breast cells ? (reply: 1)
      
1093. HTC116 cells detaching !!!! - (reply: 2)
      
1094. Revival of kasumi-1 cells - (reply: 3)
      
1095. Growth conditions for E. coli DB3.1 cells - (reply: 1)
      
1096. weird & curious problem with CHO cell culture - (reply: 3)
      
1097. HaCat do not grow after using cell scraper - (reply: 5)
      
1098. MTT assay problems (Cells just come off after incubation) - Cells just come off after incubation in 37 degree (reply: 1)
      
1099. Cell Lysates from a Western Blog - (reply: 2)
      
1100. Loss of YFP signal in transduced T cells - (reply: 7)
      
1101. Overexpression of mouse constructs in human cell lines - some questions (reply: 6)
      
1102. Freezing cell pellets and minimizing lysis - (reply: 2)
      
1103. Additional peaks in cell cycle assay - Can 37°C incubation cause clumping of fixed cells? (reply: 6)
      
1104. Total Cell protein - (reply: 1)
      
1105. Which wash buffer (to collect cell) is prefered for 2DE? - (reply: 3)
      
1106. cell culture media - glutamine! - (reply: 2)
      
1107. How many cells do you have in a confluent T 75 flask? - I have 1/2 million (reply: 2)
      
1108. can i use trypsin to collect cell sample for 2d gel analysis? - (reply: 3)
      
1109. Premature cell lysis - (reply: 2)
      
1110. urgent!!! weird Growth in cell line - (reply: 8)
      
1111. Shipping of blood cells over long distance - (reply: 1)
      
1112. refrozen cell pellet - (reply: 1)
      
1113. Drug vehicle giving toxicity in cell culture - With increse in vehicle..... toxicity decrese (reply: 4)
      
1114. contamination in cell line...... - (reply: 4)
      
1115. Neuronal Cell Culture Woes - Not even making it out of the gate! (reply: 7)
      
1116. Number of proteins per cell? - One of THESE questions... ;) (reply: 1)
      
1117. MTT with cells growhing in suspension, not adherent - (reply: 3)
      
1118. Cell ELISA - No differences between cells expressing and not, the receptor (reply: 2)
      
1119. growth curve for attached cells - (reply: 1)
      
1120. cells dont attach hard enough to 96 well - (reply: 3)
      
1121. CTLL-2 culture - preventing cell clumping - (reply: 2)
      
1122. Yet another contamination question - SF9 cells (reply: 11)
      
1123. Problems with transfection in human cells - (reply: 3)
      
1124. HepG2 cells culture conditions - (reply: 2)
      
1125. Should I use fully supplymented medium or low serum medium when treat INS1 cells - (reply: 4)
      
1126. T cell proliferation problem - (reply: 3)
      
1127. How to Handle Cells Shipped Internationally in Dry Ice? - (reply: 2)
      
1128. Differences between cells in different species - (reply: 5)
      
1129. Will pervious PBS concentration in medium have a long lasting effect on cell gro - (reply: 2)
      
1130. isotype control for GFP expressing cells? - (reply: 2)
      
1131. Trouble with growth of transformed cells - (reply: 4)
      
1132. nuclear extraction of blood cells - (reply: 2)
      
1133. Problems about detection of apoptotic cells using Annexin V/PI staining - (reply: 2)
      
1134. ELISA with undiluted cell lysate samples - (reply: 1)
      
1135. How can you expand cells if you have one dish of them? - (reply: 1)
      
1136. Androgen independent LNCaP cells - (reply: 2)
      
1137. What is the proper way to infect cell culture with virus? - (reply: 5)
      
1138. Micro-RNA profiling for differentiating cells - (reply: 1)
      
1139. Growing insect cells with other cell lines - (reply: 2)
      
1140. Candida albicans cells lysis - method (reply: 1)
      
1141. Cell cycle analysis after viral transduction - Shift of peak (reply: 1)
      
1142. Advise for Whole Cell Stain - hESC-differentiated Trophoblast (reply: 1)
      
1143. 293 T cells - (reply: 3)
      
1144. Mouse Bone Marrow Isolation - How to increase yield of BM-derived cells? (reply: 1)
      
1145. BL-21 Gold (DE3) cells and further strains - (reply: 2)
      
1146. Cell Cycle Analysis flow cytometry - How to measure desynchronisation rate of cells (reply: 1)
      
1147. Simple & straightforward question - Buffering agent for CHO cell medium (reply: 1)
      
1148. Help! Cell homogenization and protein extraction for Western Blotting - (reply: 2)
      
1149. Fixing cells before staining of surface markers - (reply: 1)
      
1150. Black dots in cell culture - (reply: 7)
      
1151. cell counting - (reply: 5)
      
1152. Optimization for cell viability assay (MTT assay) - (reply: 1)
      
1153. Anyone thought about reprogram fibroblast to liver cells? - (reply: 2)
      
1154. cell confluence in cell cultures - myoblast c2c12 in particular (reply: 2)
      
1155. cell line adherence - cells adhering to flask very tightly after transfectiono (reply: 1)
      
1156. Cell labelling - How can I label 2 cell populations? (no transgenes) (reply: 6)
      
1157. How long does it take for cells to reattach? - From balls to pancakes. (reply: 3)
      
1158. Dot plot cell cycle analysis-strange dots - (reply: 4)
      
1159. TERT transformation of fibroblast cells - Immortilising fibroblast cells. Which companies available? (reply: 1)
      
1160. Apoptosis vs Viable Cells vs Necrosis (Annexin/PI Staining) - Which quadrants to sum up? (reply: 4)
      
1161. help Splashed Raji cells on skin/face/hand - (reply: 2)
      
1162. Help! ES Lenti infection efficiency=nightmare - trouble getting over 40 % cells infected (reply: 8)
      
1163. Staining corneal endothelial cells for mechanical damage with methylen blue - (reply: 1)
      
1164. CO2 level for cell culture - (reply: 6)
      
1165. suspension cells for confocal - (reply: 1)
      
1166. RNAi is somatic and germline cells - (reply: 3)
      
1167. Luciferase and cell responsiveness - (reply: 3)
      
1168. adult cell culture - (reply: 1)
      
1169. protein overexpression in HeLa cells - (reply: 1)
      
1170. Insulin supplements to cell culture media - (reply: 1)
      
1171. After inducing with IPTG for protein expression, my cell pellet after centrifuga - (reply: 1)
      
1172. RNA/cell accumulation of difficult-to-obtain tissue - (reply: 1)
      
1173. Bacteria to Cell Culture - How many bacteria should I add to cell culture (reply: 2)
      
1174. topic and cell culture - (reply: 2)
      
1175. Running a western with cell media in gels? - (reply: 11)
      
1176. Starting cells for making one's own competent cells - (reply: 7)
      
1177. cell loss during antibody staining for flow cytometry - FACS staining protocol (reply: 5)
      
1178. Persistent stable cell line (Bos23) Contamination... - (reply: 2)
      
1179. Hematopoietic cells separation - How to get high purity and viability hematopoietic cell populations? (reply: 2)
      
1180. Transfection of large plasmid - How to get a 10kb plasmid into HEK293 cells (reply: 3)
      
1181. min6 cells immunofourescence staining - staining and culture problem (reply: 4)
      
1182. 2D-PAGE of G protein coupled receptor - Need advice on sample preparation - GPCR overexpressed in HEK293 cells (reply: 1)
      
1183. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
      
1184. Rabbit cells to grow bacteria in before immunization of rabbit for antibody prod - (reply: 2)
      
1185. rat ES cells - (reply: 2)
      
1186. Neuronal Cell Culture - Media Changes - How to change media half and half (reply: 4)
      
1187. establishment of primary cell line from biopsy - (reply: 2)
      
1188. Cell culture fasting - (reply: 1)
      
1189. mutated HeLa cells? - from cycloheximide? (reply: 1)
      
1190. Do you get insoluble proteins also in whole cell lysate? - (reply: 3)
      
1191. qPCR for transformed cells - (reply: 1)
      
1192. solution to a weird problem - pH - Adjusting pH in cell culture media (reply: 1)
      
1193. A solution to a weird problem - pH - Adjusting pH in cell culture media (reply: 6)
      
1194. descrepancy between total cell lysate and cell fractionation - (reply: 1)
      
1195. Disruption of cells using mild detergent - (reply: 2)
      
1196. Eruption of mammalian cells using detergent - (reply: 1)
      
1197. lenti virus transfected cell dies... - (reply: 4)
      
1198. Extremely Weird Issue - CHO Cell Culture (reply: 2)
      
1199. Cell Die.. Need help - (reply: 1)
      
1200. Cell scraping ruining my life - (reply: 6)
      
1201. Death of cells in the control. - (reply: 1)
      
1202. cell culture clarification - I need help please (reply: 2)
      
1203. Serum concentration in treatment of cells for MTT assay - (reply: 1)
      
1204. Minimum number of cells needed for cytometry? - (reply: 3)
      
1205. Beta-Lactam in CHO Cells - (reply: 1)
      
1206. problems with 293T cells after medium removal!!! - (reply: 6)
      
1207. B cell subset for hybridoma generation - Which B cell subset is used to make hybridomas (reply: 2)
      
1208. Propagating ccdB survival cells - (reply: 6)
      
1209. Western difficulty in primary neuron cells. - (reply: 1)
      
1210. DOubt - Competent cell (reply: 2)
      
1211. Differentiation of CD11b cells into macrophages.Help please - (reply: 1)
      
1212. Primary keratinocyte isolation- cell loss - (reply: 1)
      
1213. cells attach but don't flatten out - MDA-231 (reply: 8)
      
1214. Problem Generating Stable HeLa Cells - (reply: 1)
      
1215. Retroviral transduction of T cells - (reply: 4)
      
1216. Looking for "biocell" from the forum archives. - Cell culture contamination problems.... (reply: 1)
      
1217. p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells - p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells (reply: 9)
      
1218. Multiple nuclei?? - cell morphology - FaDu (reply: 8)
      
1219. Viral RNA is relatively stable than mRNA from cells? - (reply: 1)
      
1220. how to compensate the cell death during transfection by electroporation - (reply: 2)
      
1221. MCF-7 CULTURING - (reply: 9)
      
1222. H-ras transformed RK3E/tv-a cells - (reply: 1)
      
1223. HEK001 Cell Line and Papillomavirus - Does HEK001 cell line produce a functional papillomavirus (reply: 4)
      
1224. 2 questions: myeloma cell lines and mice MABs - (reply: 5)
      
1225. I'm getting fried-egg looking cells... What should I do?? - (reply: 8)
      
1226. Empty bubbles in the 3T3-L1 cells - (reply: 3)
      
1227. Exposing Cell Lines to Drugs Metabolized by Liver Microsomes - (reply: 1)
      
1228. UV safety in cell culture hood - (reply: 3)
      
1229. Where can I find info on the physical appearance of TB cell wall structures? - I am an artist wanting to depict the cell wall with absolute accuracy. (reply: 7)
      
1230. intracelluar staining without killing cells - (reply: 3)
      
1231. Info about A7r5 cell culture - (reply: 1)
      
1232. Continuation? Kombucha Tea and MCF-7 - (reply: 5)
      
1233. Molecular Cell Biology - Trafficking and function of a membrane enzyme (reply: 1)
      
1234. Cell lysates - Protein precipitation? - (reply: 3)
      
1235. Glucose in epithelial cell - (reply: 1)
      
1236. cell lysate for pulldown - (reply: 3)
      
1237. cell culture media - (reply: 3)
      
1238. Competent cell storage for long term use - (reply: 2)
      
1239. My RIPA cell lysis buffer - Western blot (reply: 3)
      
1240. Cell Uptake Study - (reply: 1)
      
1241. what will happen if my cell culture have exposed to unfiltered air for a few sec - (reply: 2)
      
1242. aspects of RAW264.7 cells - (reply: 5)
      
1243. anti his antibody for western blotting - high background in cell lysate western blotting (reply: 3)
      
1244. Conditioned Medium - Primary skeletal muscle derived cells conditioned medium (reply: 3)
      
1245. Lentivirus-based Knockdown for miRNAs? - for hard-to-transfect cells (reply: 10)
      
1246. Hoechst Stain does it stain dead cells - (reply: 3)
      
1247. Help for cell preparation for FACS... - (reply: 1)
      
1248. Cell Lysis Buffer - (reply: 6)
      
1249. HeLa cells and HPV - (reply: 2)
      
1250. Resolving cell growth problems using growth additives - way to resolve urgent cell growth problems (reply: 3)
      
1251. Low transformation efficiency - Rosetta-gami™ 2(DE3)pLysS Competent Cells (reply: 8)
      
1252. problem with MACS cell isolation - (reply: 5)
      
1253. cell culture split ratios. - (reply: 2)
      
1254. MDA-MB-231 cell culture issue - after thawing first passage is fine, next goes bad (reply: 7)
      
1255. blood red cells - (reply: 1)
      
1256. Attention-seeking cell cultures - reduce subculture ratio? - QM7 cells do the darndest things (reply: 2)
      
1257. NFKb signalling in cell line ??? - (reply: 2)
      
1258. kit fpr cell membrane preparation? - (reply: 2)
      
1259. Retrovirus Production - I am not getting any virus from my packaging cells! (reply: 4)
      
1260. How to find NFKb signalling is active in a cell line??? - (reply: 2)
      
1261. Fixing YFP cells - (reply: 2)
      
1262. MTT Assay: cell proliferation or cell viability??? - (reply: 4)
      
1263. Rescue RAW cell culture - RAW cells past confluent point... can they be rescued? (reply: 4)
      
1264. cell cycle arrest by colcemid - (reply: 2)
      
1265. is my lost protein is bcoz of cell death or my treatment worked??? - (reply: 2)
      
1266. CD19 Expression at various stages of plasma cell differentation - (reply: 1)
      
1267. PROLIFERATION ASSAY IN MICROTITER PLATES WITHOUT A SEMIAUTOMATIC CELL HARVESTER - (reply: 1)
      
1268. THP cells? what does THP stands for?????????? - (reply: 1)
      
1269. Immunioprecipitation from Jurkat cells?? - (reply: 1)
      
1270. Weird effect of siRNA on protein in cells - (reply: 3)
      
1271. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
      
1272. Apoptosis induction - for vero and mcf-7 cells (reply: 5)
      
1273. Need pH 6 Cell culture media - (reply: 1)
      
1274. Are my cells undergoing epigenetic changes? - (reply: 2)
      
1275. establishment of stable cell line using hek293t - (reply: 2)
      
1276. Cell pellet frozen -80 then -20 - (reply: 4)
      
1277. total cell protein vs membrane protein - (reply: 4)
      
1278. tranfection of shRNA into mammalian cells is causing the cells to die - (reply: 4)
      
1279. transfection efficiency - i am looking for a cell line that its transfection efficiency is high (reply: 4)
      
1280. Fixing the cells for FACS - Paraformaldehyde or Formaldehyde (reply: 2)
      
1281. Problem with control samples in cell IC - (reply: 3)
      
1282. Recommendation for cell cycle analysis software needed - (reply: 1)
      
1283. Tips on fermenting DH5alpha and MG1655 - e.coli cells never leave lag phase during fermentation (reply: 1)
      
1284. Cell culture contamination question - what the hell are these things?? - (reply: 3)
      
1285. 'Stringy' contamination of cells? - Weird stringy thing in cell culture flasks (reply: 3)
      
1286. Modified PBS buffer for red blood cells washing - [urgent, need opinions] (reply: 2)
      
1287. Immunofluorescence using dead cells? - (reply: 1)
      
1288. floating THP-1 cells - medium change advice - (reply: 2)
      
1289. my cells love infection, any thoughts on antibiotics? - (reply: 3)
      
1290. Culture Media for stem cells - (reply: 1)
      
1291. Trypsinization or Scraping for adherent cells in ChIP assay? - (reply: 8)
      
1292. Anyone heard of cells spontaneously fluorescing red?? - (reply: 4)
      
1293. Freezing cells - How long can I keep cells at -80C? (reply: 6)
      
1294. Help regarding cell line - (reply: 1)
      
1295. organizing cell lines - (reply: 1)
      
1296. Cell plating density - (reply: 3)
      
1297. Murine antibodies - A panel of antibodies for murine blood cells (reply: 2)
      
1298. transfecting cells with mature miRNA or precursor miRNAs? - (reply: 5)
      
1299. MDA-MB-231 cells question - (reply: 3)
      
1300. thawed cells wont adhere - pc3 and LNcaP (reply: 2)
      
1301. DiFi Cell line (colon carcinome) - Where can I get it???? (reply: 1)
      
1302. HaCaT cells - (reply: 2)
      
1303. Why does my media turn alkaline not acidic? - MCF-7 in EMEM (reply: 4)
      
1304. lncap and MCF-7become ethanol sensitive? - are lncap cells and MCF-7 cells ethanol-sensitive? (reply: 2)
      
1305. Dying endometrial stromal cells - (reply: 1)
      
1306. Help me - Are my cells infected with something or could this be unsusual morphol - (reply: 5)
      
1307. how to dissociate cells for cell migration assay - (reply: 3)
      
1308. soft agar assay - cells in the top agar cells adhere to the plates unexpectedly. (reply: 4)
      
1309. RNA extraction - how many cells do I need at least?? (reply: 4)
      
1310. RNA extraction protocol for small amounts of cells - (reply: 2)
      
1311. about estrogen receptor beta negtive cell lines - can anyone give me some suggestion about ER beta negtive cell lines? (reply: 1)
      
1312. Problems when harvested cells with SDS loading buffer - extremely sticky & formed clumps (reply: 4)
      
1313. strange morphology of sp2/0 cells after fusion!!! Please help! - morphology switch from round to triangular!!!! (reply: 2)
      
1314. fabrablast primery cell line problem - how to detach cell well (reply: 1)
      
1315. what solvent is better compatible for most cell lines? - DMSO vs methanol (reply: 3)
      
1316. Cell growth rate & O2 consumption - (reply: 2)
      
1317. I did not change the media the day after transfecting 293T cells. Are they going - (reply: 1)
      
1318. Cells stick to dish in Embryoid Bodies - (reply: 1)
      
1319. Changing the media after transfection of 293T cells - I did not change the media the day after transfecting 293T cells. (reply: 7)
      
1320. Non Mouse / Human Cell Line that is easily transfected - (reply: 2)
      
1321. how to plate cells precisely - (reply: 1)
      
1322. RNA extraction from stained cells - (reply: 2)
      
1323. Methods to assess cell viability - (reply: 1)
      
1324. suspension cell transient transfection - (reply: 10)
      
1325. Adherent cells washing off in 96 well -MTT - (reply: 4)
      
1326. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
      
1327. plasmids - plasmids for stable cell line construction (reply: 2)
      
1328. abnormal nuclear morphology after treating shRNA - confocal, shRNA, nuclear morphology, cell division (reply: 2)
      
1329. average total protein content per 293t cell - (reply: 2)
      
1330. T cells preparation for FACS - (reply: 1)
      
1331. counting cells - (reply: 3)
      
1332. Cell OD and spec problems - (reply: 1)
      
1333. cell confluency & effect on cells...?? - (reply: 4)
      
1334. Fixing cells for flow cytometry - alternative to 1% paraformaldehyde (reply: 3)
      
1335. Washing Red Blood Cells - Tips on washing red blood cells (reply: 8)
      
1336. MEF Cell marker - (reply: 1)
      
1337. (CELL CULTURE) Serious advice needed regarding my career..? - (reply: 2)
      
1338. Change of colour in cell culture media...? - (reply: 2)
      
1339. cell count - how to calculate (reply: 2)
      
1340. generating cell line knockouts - (reply: 3)
      
1341. Cancer Stem Cells?? - (reply: 10)
      
1342. Some kind of contamination? - mouse transduced cell line (reply: 7)
      
1343. Cell stimulation of transcription - (reply: 5)
      
1344. Mouse blood density/osmolarity of blood cells for separation - (reply: 5)
      
1345. suitable cell lysis buffer - (reply: 1)
      
1346. Toluidine blue staining for cell culture - (reply: 1)
      
1347. cell culture - 3D cell culture (reply: 1)
      
1348. Aspergillus in cell culture room - HELP!!! how hardcore to decontami - Cell culture, contamination (reply: 6)
      
1349. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
      
1350. MEF culture contamination? - Contaminated cell culture? (reply: 2)
      
1351. very simple question about hemocytometer cell counting - (reply: 1)
      
1352. nucleic acid extraction from silenced cells - (reply: 6)
      
1353. direct lysis of cell pellet - How to get rid of DNA? (reply: 3)
      
1354. In Vitro Drug Exposure Cytotoxicity Testing and Cell Confluency - (reply: 8)
      
1355. Dendritic cells - Protocol of stimulation of dendritic cells (reply: 3)
      
1356. giant cells in HEK293 cell line - (reply: 10)
      
1357. Plant cell transfection - (reply: 1)
      
1358. stable cell line - selection after retroviral infection (reply: 1)
      
1359. Storing cell lines long-term at -70C - (reply: 4)
      
1360. Commercially available primary mouse endothelial cell line? - (reply: 2)
      
1361. Frozen cells in -80 - (reply: 1)
      
1362. Plant or Fungal Cell Dissociation Technique? - (reply: 3)
      
1363. how can i bring cells onto a slide? - ...for microscopical observation! (reply: 7)
      
1364. cell density to be seeded? - cytotoxicity studies (reply: 1)
      
1365. Lysis of the cells - (reply: 1)
      
1366. Cells do not lyse in SDS buffer - (reply: 1)
      
1367. Storage of Fixed Cells for Immunofluorescence staining - (reply: 1)
      
1368. lysis buffer for lymphoblast cells - (reply: 1)
      
1369. problem with cell cycle analysis - (reply: 3)
      
1370. THP-1 Cells differentiation - THP-1 cells differentiated using IFN-gamma/LPS (reply: 7)
      
1371. Single cell suspension from solid tumor - (reply: 3)
      
1372. cell aggregation? - (reply: 1)
      
1373. T-Cell receptor - Is it a single type on a T cell or multiple types? (reply: 1)
      
1374. cells weak adhesion - detachment (reply: 4)
      
1375. how we count the passages in cell cultures? - (reply: 5)
      
1376. resolving cell growth problems - (reply: 2)
      
1377. clumping of cells - (reply: 7)
      
1378. stability of cell suspension - (reply: 1)
      
1379. cell adhesion in serum free condition - (reply: 1)
      
1380. effects of cell confluency - (reply: 1)
      
1381. Processing cell samples for low abundance proteins - direct lysis with SDS-PAGE loading buffer (reply: 2)
      
1382. Good Cell line for Histone 3 and 4 - Positive Control for Basic Staining and WBs (reply: 1)
      
1383. polarized cells - (reply: 4)
      
1384. PCNA (proliferating cell nuclear antigen) - is it an adequate marker of proliferating cells? (reply: 2)
      
1385. cell adhesion assay - using collagen (reply: 1)
      
1386. Problem clariflying/filtering insect cell lysate - (reply: 2)
      
1387. cell culture urgent!! - (reply: 2)
      
1388. Moving S2 cells from one plate to another - (reply: 1)
      
1389. Cell culture medium instead of Nucleofector solution?? - (reply: 3)
      
1390. Nucleofection of ES cells, medium instead of nucleofector solution? - (reply: 1)
      
1391. mononuclear cells medium - I am stuck with the concentration of 2-mercaptoethanol (reply: 1)
      
1392. Accidental thawing of frozen cells - (reply: 3)
      
1393. how to start with a new cell line ? - how to start with a new cell line ? (reply: 1)
      
1394. HaCaT cells die after trypsinization - (reply: 1)
      
1395. Transporting some bacteria cells - transport bacteria cells pellet (reply: 2)
      
1396. GFP and Luciferase Cell Line - (reply: 2)
      
1397. Transfecting primary cells with plasmid - (reply: 1)
      
1398. transfecting and selecting EL4 cells - (reply: 1)
      
1399. Induce Osteoblast lineage from C2C12 myoblast cells - (reply: 1)
      
1400. Lentivirus production with LNCX vector - Do I have to use Packaging cell lines? (reply: 3)
      
1401. Issue on mycoplasma detection and treatment in cell culture - (reply: 3)
      
1402. Transfection into eukaryotic cells - (reply: 1)
      
1403. Fixing cells with PFA - (reply: 1)
      
1404. A549 cells not doubling - has anyone been through this with other cell lines? (reply: 4)
      
1405. Immunofluorescence in HEK cells issues - (reply: 1)
      
1406. cell sorting - losing cells? - (reply: 3)
      
1407. What if my expressed protein is toxic for the cells... - (reply: 1)
      
1408. competent cells - difference between electroporation and chemical comp. cells (reply: 1)
      
1409. Possible contamination of leukemic cell culture - debris, growth inhibition, cell aggregates (reply: 3)
      
1410. human cancer cell line splashed into my eyes - (reply: 3)
      
1411. Saving tissue and single cell suspension - FACS, single cell suspension (reply: 1)
      
1412. BL21(DE3)LysS competent cells - (reply: 1)
      
1413. ATP turnover in neuron - ATP hydrolysis rates in neuronal cells (reply: 2)
      
1414. Adhering CHO cell culture - cells don't adhere! (reply: 2)
      
1415. do e.coli cells get ruptured during high spin - (reply: 1)
      
1416. Please Help with COS-1 cells^^ - (reply: 1)
      
1417. IPTG Induction and cell lysis issues - pET 21b system (reply: 1)
      
1418. cell counting - to get an impression when my cells are in the log phase (reply: 1)
      
1419. Whole cell lysis of fibroblasts - (reply: 1)
      
1420. Whole cell lysis of fibroblasts - (reply: 1)
      
1421. test of cytotoxic effect of compounds - why cells are pretreated with actinomycin C ?? (reply: 1)
      
1422. Proliferation Assay - Should cells be in the logarithmic phase ? (reply: 3)
      
1423. cell density and growth - (reply: 5)
      
1424. Laser capture microdissection - capturing single cells (reply: 2)
      
1425. MCF-7 in cytotoxicity assay - (reply: 2)
      
1426. when cells have to be synchronized?? - (reply: 2)
      
1427. cells in RNAlater - the title say`s it all :) (reply: 4)
      
1428. Cytotoxicity assay for adherent cells exposed to particles - (reply: 1)
      
1429. Murine Cell Lines - (reply: 2)
      
1430. Cell Proliferation: To adhere or not to adhere (before treatment), that is the q - (reply: 2)
      
1431. why are some cell lines more popular than others?? - (reply: 4)
      
1432. NK cell staining - (reply: 4)
      
1433. cell culture media - with or w/o L-glutamine? (reply: 2)
      
1434. Measuring DNA, RNA, protein and cell wall synthesis - (reply: 1)
      
1435. pro-apoptotic effect of compounds on cancer cell lines - which cell line to test the non-toxic effect? (reply: 3)
      
1436. Question in Generating double stable cell line - (reply: 3)
      
1437. activity of sex-chromosome in somatic cells - (reply: 5)
      
1438. CaCl2 for making competent cells - (reply: 3)
      
1439. Calcium Phosphate Transfection for CV1 CELLS - (reply: 2)
      
1440. ICC Cell Adherence Issue - (reply: 1)
      
1441. gentamycin resistance in human cencer cell culture - please suggest the alternative antibiotic (reply: 2)
      
1442. siRNA control (scramble seq) causing cell death - what to try next? (reply: 2)
      
1443. How to normalize my samples before I transfect my cells - (reply: 6)
      
1444. Isolation of intestinal epithelial cells - Isolation of intestinal epithelial cells (reply: 2)
      
1445. Number of proteins expressed in cell - (reply: 2)
      
1446. Do HEK 293 cells contain functional E1A/E1B? - (reply: 2)
      
1447. Sodium Pyruvate - What is it's use in (NIH 3T3) cell culture? (reply: 4)
      
1448. Calcium phosphate method vs lipofectamine - troublemaker neuroblastoma cells (reply: 7)
      
1449. Which cell lines are best for transient transgene expression? - (reply: 3)
      
1450. Alzheimer's cell model - (reply: 7)
      
1451. Partial cell subculture? - (reply: 2)
      
1452. No difference between transfected and untransfected cells - Using eGFP siRNA on GFPHEK293 cells usign flurimeter (reply: 5)
      
1453. MCF-7 passage number - (reply: 1)
      
1454. Whole Kidney primary cell culture - (reply: 1)
      
1455. dh5 alpha competent cells? - (reply: 2)
      
1456. which is the best lysis buffer for whole cell extracts? - (reply: 1)
      
1457. Please help me to understand- cell counting - (reply: 1)
      
1458. Preparing competent cells under laminar flow? - or on the bench sufficient? (reply: 5)
      
1459. Cell count - Please help - (reply: 11)
      
1460. What is DME medium for Hybridoma cell culture - is DME medium and DMEM medium are the same for hybridoma culture?? (reply: 1)
      
1461. Cell Count - (reply: 1)
      
1462. Generating stable cell line - (reply: 2)
      
1463. Harvesting cells off of collagen gels - (reply: 1)
      
1464. cells can't be lysed after crosslinking - (reply: 4)
      
1465. How many cells to seed? - simple question :) (reply: 5)
      
1466. Is there a problem by adding HCL to the cell culture medium in order to manipula - (reply: 1)
      
1467. mouse macrophage cell line - (reply: 5)
      
1468. Problem clarifying lysate from Hi5 insect cells - (reply: 1)
      
1469. cell line information - require (reply: 1)
      
1470. Urgent problem - Media change = cells die in 48h (reply: 3)
      
1471. recombineering for ES cell targeting - retrieval problems (reply: 1)
      
1472. Rat monocyte cell line - (reply: 1)
      
1473. How often do you thaw new cells? - passage number or time? (reply: 7)
      
1474. Does high speed centrifugation destroy viable cells? - (reply: 2)
      
1475. Cell culture - (reply: 7)
      
1476. Problems when plating neuroblastoma cells - (reply: 3)
      
1477. Is pFLAG expression toxic? - FLAG seems to kill my HeLa cells. This can't be right (reply: 1)
      
1478. Cell Doubling Time - (reply: 3)
      
1479. Dimethyl Sulfate (DMS) as carrier in cell cultures - (reply: 1)
      
1480. Amount of fixation buffer - is it dependent on the number of cells? (reply: 2)
      
1481. synchronized cells or non-synchronized cells - (reply: 3)
      
1482. pre-prepararation of competent cells - (reply: 3)
      
1483. ROS accumulation - ROS reactive oxygen species accumulation in cells (reply: 3)
      
1484. Bacterial invasion assay with human cells - (reply: 2)
      
1485. Measuring gene expression across cell lines - (reply: 2)
      
1486. How to make an isotonic solution for h9c2 cells - (reply: 2)
      
1487. migration < invasion??? - More cells migrating through matrigel than through control inserts (reply: 1)
      
1488. Zeocin of G418. Which is the better selection agent for CHO cells? - (reply: 1)
      
1489. Immunofluorescence with transfected cells - (reply: 3)
      
1490. Cloning Problem (cells grow but no plasmid isolated) - (reply: 13)
      
1491. Problem: protein precipitates from cell lysate after defrosting - (reply: 4)
      
1492. Contamination in cell cultures - (reply: 9)
      
1493. DMSO killing more cells at lower concentrations - (reply: 1)
      
1494. frozen competent cells - how to prepare competent cells from frozen bacteria? (reply: 3)
      
1495. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
      
1496. lysing cells on ice - (reply: 2)
      
1497. Fibroblast cell culture - (reply: 1)
      
1498. Cardiac stem cells - (reply: 3)
      
1499. How to visualize tumor cells in mice with out sacrificing the animals - (reply: 3)
      
1500. cell count and dilution - (reply: 1)
      
1501. z competent cells - (reply: 2)
      
1502. immunofluorescence staining of sperm cells - (reply: 2)
      
1503. Cell Culture procedure(manual, protocole, what ever ;) - (reply: 1)
      
1504. colony formation assay - how many cells to seed (reply: 2)
      
1505. Primaria/gelatin plates and gravity-defying fixed cells - (reply: 2)
      
1506. Contaminations in cells cultured in suspension - (reply: 2)
      
1507. suspension cell transient transfection - (reply: 2)
      
1508. What is this ? - Cell culture contaminant (reply: 2)
      
1509. cell cryopreservation after siRNA transfection - (reply: 1)
      
1510. Cell Digestion Protocol - (reply: 1)
      
1511. spin hek cell lysate after adding laemmeli buffer? - (reply: 1)
      
1512. cell growth - (reply: 4)
      
1513. primary cells could not attch to regular polystyrene flasks but immortalised cel - does anybody know what could be happening (reply: 1)
      
1514. How to culture cells after sorting? - (reply: 3)
      
1515. How to kill nerve cells in a living hydrozoans? - Trying to procude partial/complete nerveless jellyfish (reply: 3)
      
1516. lifespan of primary cells in vitro - how long can different cell types survive (reply: 3)
      
1517. measuring apoptosis - reduced cell proliferation due to apoptosis (reply: 1)
      
1518. T cell differentiation protocols - Th1/Th2/Treg/Th17 (reply: 4)
      
1519. Mitochondria extraction from human liver cells - (reply: 1)
      
1520. lipofectamine and PEI transfection of hela cells - (reply: 2)
      
1521. What can I do whit my Cancer Stem Cells?(I know, its a big question..) - (reply: 7)
      
1522. Wash L929 Cells with DPBS (with or w/out CA MG?) - DPBS with or W/out CA,MG? (reply: 2)
      
1523. Especially tenacious cells -- what to do? - (reply: 9)
      
1524. MDCK cell vascuolation - I got my MDCK cell growing in the T-75 flask (reply: 5)
      
1525. Culturing HeLa Cells - (reply: 2)
      
1526. expression of His-tagged protein in insect cells - (reply: 2)
      
1527. How to isolate vascular smooth muscle cell? - (reply: 1)
      
1528. Whole cell lysate including nucelar proteins? - (reply: 3)
      
1529. Colony PCR with Eukaryotic Cells? - Any Experiences? (reply: 4)
      
1530. HELP with cell lysis for DNA assay - Something is floating in my lysate solution!!! (reply: 1)
      
1531. culturing MCF-7 cellls - clumping and maintaining healthy cells (reply: 3)
      
1532. Cell culture media - require information on media (reply: 3)
      
1533. how to measure ROS in cell wiht H2DCFH - (reply: 4)
      
1534. VEGF in cell nucleus - (reply: 2)
      
1535. cell treatment rules - (reply: 4)
      
1536. method to seed cells in hyrogels - cell seed in hydrogels (reply: 1)
      
1537. transfecting human mesynchemal stem cells - (reply: 5)
      
1538. cell schock - (reply: 2)
      
1539. Flow cytometry of RFP and CFP expressing yeast cells - (reply: 1)
      
1540. Can you store cell pellets for future nuclear extraction? - (reply: 5)
      
1541. using cell lysate to run native gel? - (reply: 1)
      
1542. Cell Suspension from Frozen Tissue? - (reply: 6)
      
1543. looking for stromal cell marker - (reply: 1)
      
1544. 'Black dots' in cell culture - (reply: 2)
      
1545. ChIP in WT and KO cells - how to analyze - (reply: 1)
      
1546. Mouse T cell line - (reply: 1)
      
1547. Making competent cell from freeze-thawed stock - (reply: 2)
      
1548. Invasion assay using matrigel- cell clumps - (reply: 1)
      
1549. producing cell lines - why viruses ?? (reply: 4)
      
1550. What color should the cell pellets be after sonication and centrifugation? - (reply: 1)
      
1551. Please help--possible bacteria issue--cholesterol + red blood cells involved - (reply: 1)
      
1552. Cell Lysis for DNA extraction - Not every sample is successful (reply: 5)
      
1553. How to detach an antibody from cell surface - (reply: 4)
      
1554. Water for electrocompetent cells - (reply: 2)
      
1555. Gp 293 Luc cells - (reply: 1)
      
1556. how to check cell viability - (reply: 1)
      
1557. Starter culture dilution (preparation of competent cells) - I don't know how much should I dilute my starter culture... (reply: 3)
      
1558. cell fixation - (reply: 1)
      
1559. cell detachment in IHC - can I incubate cells with hoechst, heparin and PFA at the same time? (reply: 1)
      
1560. cell immobilization for confocal - (reply: 1)
      
1561. problem with 293T cell co-transfection - (reply: 5)
      
1562. Transfection and WB - Transfected cell line shows incorrect bands on WB (reply: 2)
      
1563. Cell Death after formaldehyde treatment - Immunofluorescence (reply: 5)
      
1564. Cell Densitometry - (reply: 1)
      
1565. My cells died after changing media... - (reply: 6)
      
1566. Help for an Organic Chemist - Question about bacteria cell wall permeability. (reply: 6)
      
1567. H9C2 cells - trouble when culturing H9C2 cells (reply: 4)
      
1568. Contamination of cell ?? - (reply: 2)
      
1569. Stem cells, primary culture - Literature - What are some good papers to read to get started in this area? (reply: 3)
      
1570. nonspecific band in transfected c-abl cells - nonspecific band in transfected c-abl cells (reply: 1)
      
1571. Passaging Cells - (reply: 4)
      
1572. Modifying collagen stiffness / rigidity in 3d cell culture - (reply: 1)
      
1573. Mysterious cell death - need Hela Help! - (reply: 1)
      
1574. NSC-34 Cells sloughing off at media change - (reply: 4)
      
1575. Chosing the right competent cells - (reply: 1)
      
1576. Cell proliferation assay for LNCaP cells - (reply: 4)
      
1577. liquid nitrogen in cell culture - (reply: 2)
      
1578. differential cell counting in mixed cultures - (reply: 1)
      
1579. Questions about animal cell cultue Please answer - (reply: 1)
      
1580. immunofluorescence for HUVEC - how to do immunofluorescence for cancer cells (reply: 3)
      
1581. membrane type of cell culture filtration - (reply: 3)
      
1582. Critical column of media above cells for access of oxygen - (reply: 1)
      
1583. EDTA bacterial cell lysate - (reply: 2)
      
1584. Is MTS Assay suitable for Adherent cells? - Confused about MTT Assay and MTS assay.. (reply: 1)
      
1585. membrane damaged = cell death? - (reply: 2)
      
1586. 293 cells help! - (reply: 1)
      
1587. stable cell line - (reply: 2)
      
1588. Color of cryopreserved cell line - (reply: 1)
      
1589. Established (tumor) cell line - or not...? - (reply: 3)
      
1590. Culturing cell in absence of any growth factors - Cell culture minus growth factors (reply: 1)
      
1591. proliferation assay for small cell population - (reply: 2)
      
1592. Cell fixation with glutaraldehyde - (reply: 3)
      
1593. Yeast contamination in cell culture - hood to blame? - (reply: 6)
      
1594. Comparing cell # or DNA content - of pelleted cells (reply: 1)
      
1595. bicarbonate in cell culture: powder v. liquid? - (reply: 3)
      
1596. cell adhesion assay - collagen (reply: 1)
      
1597. To break cell clumps - suspension culture related (reply: 4)
      
1598. Problems for MTT in SH-SY5Y cell line - (reply: 1)
      
1599. cell stainer size - (reply: 2)
      
1600. FUNGI SILENCING - PROTOCOL TO TRANSFORM FUNGI CELLS (reply: 2)
      
1601. Difficulties with the transfection of cells CHO DG44 - (reply: 9)
      
1602. contamination in establishing stable cell lines - (reply: 1)
      
1603. How much cell medium for 1 week of culturing? - (reply: 1)
      
1604. p53 +/- matched-pair tumor cell lines? - (reply: 2)
      
1605. Tranfection cell density and overgrowth - (reply: 3)
      
1606. shRNA selection in suspension cell culture - (reply: 6)
      
1607. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
      
1608. Ethanol/methanol/dmso for cell culture - (reply: 1)
      
1609. stored cytokines from supernatant cell culture - stored cytokines from supernatant cell culture (reply: 3)
      
1610. Rapid Cell Count (in vivo) - (reply: 5)
      
1611. cell migration assay (corning transwell) - (reply: 2)
      
1612. cell culture in 2 stack flask - (reply: 1)
      
1613. Invitogen cell countess - (reply: 5)
      
1614. Hypoxia Treatment For Cell Culture - (reply: 4)
      
1615. Picking strain for e coli competent cells - (reply: 6)
      
1616. cell death - can anybody tell me what process this looks like (reply: 3)
      
1617. Liver cancer cell transfection via lipofectamine - Liver cancer cell transfection method (reply: 3)
      
1618. Marker do discriminate cell cycle phases - G2 phase / Mitosis (reply: 3)
      
1619. hematopoietic cell lines - (reply: 3)
      
1620. Cos7 Cells to produce retrovirus - (reply: 2)
      
1621. how to make nonadhensive cells attach to coverslips without affecting cellular s - (reply: 1)
      
1622. Constructing puromycin kill curve - How to count the number of adherent cells without detaching them? (reply: 3)
      
1623. stable cell line generation - (reply: 5)
      
1624. histological staining for plant cell wall components - (reply: 1)
      
1625. My adherent cells are detaching - (reply: 2)
      
1626. Cell culture media - DMEM and DMEM/F-12 (reply: 1)
      
1627. Cell Cycle analysis of proliferating lymphocytes - (reply: 1)
      
1628. Problems with HEK293 cells in 96 well plate format - 293 cells do not adhere well to the plate (reply: 1)
      
1629. Cell Synchronization - (reply: 1)
      
1630. Slow cell culture growth? - (reply: 3)
      
1631. Protein quantification from paraformaldehyde-fixed cells - (reply: 2)
      
1632. cell lysis buffer - (reply: 1)
      
1633. cell aging - cell culture (reply: 2)
      
1634. cell concentrations - (reply: 4)
      
1635. Floating cells...pls help - (reply: 6)
      
1636. Cell migration assay with 3 cell types - (reply: 1)
      
1637. Whole Genome Methylation Analysis Feasibility - Methylation analysis on DNA from rare cell populations (reply: 3)
      
1638. How many B cells do I need to get a 1mg of protien - (reply: 2)
      
1639. Do histology staningings work in cell culture? - (reply: 2)
      
1640. Stimulating/Drugging Mammilian Cells - What is the proper way to add drugs or stimulants to a cell culture? (reply: 3)
      
1641. T cell transduction - (reply: 3)
      
1642. compound that block DNA replication in human cell - (reply: 1)
      
1643. Tranfection of HL 60 cells - (reply: 1)
      
1644. mouse liver cell line? - (reply: 1)
      
1645. Failed Transformation of Tuner (DE3) cells - (reply: 1)
      
1646. Cells suddenly died after changing the medium... why? - (reply: 4)
      
1647. type of rat hepatic cell for toxicology study - seeking opinion on what type of cell to be used (reply: 1)
      
1648. Food colouring agar plates - Do you think it will affect cells? (reply: 11)
      
1649. CHO Cell contamination - (reply: 6)
      
1650. solve MCF-7 culture trouble - (reply: 4)
      
1651. MDCK cells vacuolation - Vacuolation of MDCK cells while staining (reply: 4)
      
1652. seperating cells and antibodies - Separating cells and antibodies using centrifugation (reply: 1)
      
1653. Neuronal precursor cells - (reply: 1)
      
1654. Sticky cells - (reply: 2)
      
1655. Having problem establishing stable cell line...... - (reply: 5)
      
1656. Transferring mamallian cell colonies for selection - (reply: 2)
      
1657. Antibody titration and Cell number - (reply: 1)
      
1658. Cell Invasion Assay - possible to analyze with flow cytometry? (reply: 5)
      
1659. Cell culture contamination - "outsider I.D." (reply: 2)
      
1660. Alternative media for PC-12 cells - (reply: 2)
      
1661. viability of adherent cells - (reply: 3)
      
1662. How long can viably frozen cells stay good in -80 degree? - (reply: 5)
      
1663. Trypsin the cell killer - How much death does trypsin actually cause (reply: 2)
      
1664. How to grow cell in the iron-deficient environment? - 4T1 cell. (reply: 1)
      
1665. HeLa cells and BCL 2 expression - (reply: 1)
      
1666. B104 cells - (reply: 2)
      
1667. SH-SYS5 cell culture for dummies... - (reply: 4)
      
1668. collagen staining in cell culture - (reply: 5)
      
1669. Hematopoietic lineage depletion kit - Hematopoietic stem cell enrichment kits (reply: 4)
      
1670. changing cell shape - (reply: 2)
      
1671. how to change cell shape? - (reply: 3)
      
1672. About T-antigen and tumor cell lines - (reply: 2)
      
1673. Sub-culture of N2a cells - (reply: 1)
      
1674. Haematopoietic stem cells - (reply: 5)
      
1675. CHO cell line - To use or not to use - - incubator ran out of CO2 (reply: 1)
      
1676. proteomics - minimum cell number (reply: 1)
      
1677. Standard Curve for OD Turbidity vs Cell density - (reply: 6)
      
1678. Cells to Ct RNA isolation kit (Ambion) - (reply: 1)
      
1679. Plasmids for protein expression in mammalian cells - (reply: 2)
      
1680. cell lysis after induction - (reply: 1)
      
1681. competent cell preparation? - (reply: 1)
      
1682. One Shot® ccdB Survival™-T1R Chemically Competent Cells: Gateway cloning???? - problems using competent cells for destination vectors in gateway clon (reply: 5)
      
1683. Dendritic cell infection - (reply: 2)
      
1684. Competent Cells - (reply: 5)
      
1685. looking for bovine skeletal muscle cells - (reply: 5)
      
1686. my cell line doesnt change the colour of the media - even when cells are 100 % confluent (reply: 4)
      
1687. Dead Dendritic cells ... please help - (reply: 1)
      
1688. Cell concentration Question - (reply: 3)
      
1689. cho cell line adoptation to serum free medium - (reply: 1)
      
1690. Problems with viability of Jurkat's cells - (reply: 3)
      
1691. THP-1 cell surface markers for Flow Cytometry - Need a robust marker for undifferentiated THP-1 cells (reply: 1)
      
1692. 24 well HeLa Cell Total Protein Concentration? - What would it be (reply: 2)
      
1693. Gene expressed in lenti-packaging cells, but not in lenti-infected cells - (reply: 2)
      
1694. Culturing U2OS cells - (reply: 1)
      
1695. staph cell count - (reply: 5)
      
1696. What kind of speed for HepG2 cells. - (reply: 2)
      
1697. Serum free culture media 293T cells? - (reply: 1)
      
1698. Embryoid bodies - How to differentiate mouse ES cells quickly and efficiently (reply: 8)
      
1699. cell count - is it right? (reply: 3)
      
1700. Haemocytometer cell count - Coverslips (reply: 4)
      
1701. Growing two cell types in the same flask?? - (reply: 1)
      
1702. Cell isolation - (reply: 1)
      
1703. trouble to grow cells on top of coverslip - (reply: 6)
      
1704. about measuring luciferase activity in living cells - (reply: 9)
      
1705. Culture conditions for cell treatments - (reply: 4)
      
1706. preadipocyte 3T3-L1 cells question? - (reply: 1)
      
1707. SH-SY5Y cell culture - Suspended cells (reply: 4)
      
1708. Magnetic-activated cell sorting ? - (reply: 4)
      
1709. mRNA level in transgene cell line or knocking down cell line - (reply: 3)
      
1710. How to increase IF signal in acetone/methanol fixed cells? - (reply: 5)
      
1711. Macrophage cell culture - Strange particles persist in an otherwise healty looking MF culture (reply: 5)
      
1712. sheep red blood cell rosetting protocol - (reply: 1)
      
1713. Tranformation cell harvesting - (reply: 5)
      
1714. self-renewal Vs proliferation - stem cells (reply: 3)
      
1715. Plyse cell and DH5a - (reply: 1)
      
1716. Brain tumour tissue enzymatic digestion for culturing cells - (reply: 2)
      
1717. INS-1 cell line - (reply: 1)
      
1718. Central cell seeding - (reply: 4)
      
1719. please help with Freezing primary cells - (reply: 1)
      
1720. COMPETENT CELL PREPARATION - (reply: 6)
      
1721. MCF-7 cells dying after siRNA transfection + estrogen - (reply: 1)
      
1722. suggest cells expressing low levels of adhesion molecules - (reply: 1)
      
1723. Cell Culture and Pipette Tips - (reply: 4)
      
1724. Flow cytometry CD14 staining very dim - Bone marrow cells CD14 staining for flow cytometry (reply: 3)
      
1725. Cell counting issues - the ring of death on my flasks (reply: 5)
      
1726. Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
      
1727. Which cell line culture has endogenous Wnt1 expression? - (reply: 2)
      
1728. RNA Isolation - RNA isolation from human cell lines (reply: 2)
      
1729. Cell Fixation - methanol fixation of cells (reply: 3)
      
1730. How to retrieve my human blood immune cells from liquid nitrogen without killing - (reply: 3)
      
1731. stem cells and EB (Embryoid bodies) - (reply: 1)
      
1732. Immunohistchemistry of red blood cells - (reply: 1)
      
1733. cell cryosection - (reply: 5)
      
1734. Shifting of entire cell cycle to the left - (reply: 3)
      
1735. freezing suspended cells - (reply: 7)
      
1736. Cell Brakeage...how will I know - I need the mitochondria. (reply: 1)
      
1737. How many cells... - ...gives you how much RNA? (reply: 2)
      
1738. Cell culture - Culture medium (reply: 1)
      
1739. Anybody ever use whole blood plasma serum stimulate cells? - (reply: 2)
      
1740. How can I separate my cell line from feeder cells - (reply: 3)
      
1741. ChIP-seq: Minimum number of cells - (reply: 3)
      
1742. CHO or VERO? - Cell culture (reply: 2)
      
1743. Media for squamous cells - (reply: 3)
      
1744. how to trace single cell movement throughout gastrulation of zebrafish - (reply: 4)
      
1745. Oxygen diffusion through cell culture medium - (reply: 2)
      
1746. Request for Mono Mac-1 cell line - (reply: 1)
      
1747. help: bovine Intestinal Epithelial Cell Culture protocol - (reply: 4)
      
1748. How much sonication power needed? - protein extraction from cells (reply: 2)
      
1749. protein extraction - from cell line in T25 flask (reply: 5)
      
1750. Getting cells from flasks - (reply: 5)
      
1751. transformation efficiency decreased - storage conditions of competent cells (reply: 7)
      
1752. Fixation of immune cells - "how to" protocol needed (reply: 2)
      
1753. cell cycle analysis by FACS - (reply: 2)
      
1754. Variable expression of antigen in various subcellular locations - Any ideas why? - ICC Staining on Glioma Cells (reply: 2)
      
1755. cell cycle analysis PI staining shift - (reply: 1)
      
1756. Lipofectamine transfections once working now dying - Optimised transfection in HEKs starting to cause cell death (reply: 6)
      
1757. Problem with stable overexpression cell lines - (reply: 3)
      
1758. seeking help in stable cell line generation - (reply: 3)
      
1759. SDS, Cell numbers, dilution - (reply: 18)
      
1760. Help with Hep G2 cells - (reply: 1)
      
1761. Floating cells in adherent VERO culture - contamination? (reply: 2)
      
1762. RAW264.7 cells+nitric oxide determination - (reply: 13)
      
1763. BL 21 cells - (reply: 4)
      
1764. Mutated MDCK cells - protocol for mutating MDCK cells (reply: 1)
      
1765. Preparing mutated MDCK cells - method of mutation of MDCK cells (reply: 1)
      
1766. Cell pellet pesuspension without Vortexing or pippeting - seeking advice - (reply: 2)
      
1767. western blot problem: cell culture VS mouse tissue - (reply: 4)
      
1768. How long are cells viable in PBS? - (to make trypan blue exclusion assay less tedious) (reply: 3)
      
1769. Detachment of very adherent cells for flow cytometry - (reply: 7)
      
1770. THP-1-Blue-CD14 cells - (reply: 4)
      
1771. MCF-7's not responding to estrogen - (reply: 5)
      
1772. are cells prone to swelling in PBS after sorting - (reply: 3)
      
1773. Is it Possible - Is it possible RNAi cross-reaction with normal cells molecules?? (reply: 1)
      
1774. suspension cell culture - optimum cell number in plates (reply: 2)
      
1775. Whole tissue starting product - not cultured cells - (reply: 2)
      
1776. DNA extraction fron HSV-1 infected Vero cell - (reply: 2)
      
1777. How fast do DH5-alpha cells with plasmid insert lyse after defreezing? - (reply: 1)
      
1778. Overnight SURE cell culture in LB-carbenecillin - (reply: 4)
      
1779. culturing COS-7 cells - (reply: 5)
      
1780. Would transfected plasmids bind histones in HeLa cells? - h3k4 demethylase LSD1 (reply: 3)
      
1781. Loss of cell adherence after transfection - The first passage after selection process cells lost adherence (reply: 2)
      
1782. how will i be able to culture tumor cells? - (reply: 3)
      
1783. General cell lysis question - whole cell lysates need to be centrifuged? (reply: 2)
      
1784. Rnase for cell cycle - (reply: 5)
      
1785. Transfection Issue - Are MCF-7 & MDA-MB-231 cells generally difficult to transfect? (reply: 2)
      
1786. cell loss upon centrifugatino - (reply: 10)
      
1787. cell lines vs tissue expression - (reply: 1)
      
1788. Is molecular biology grade DMSO good enough for cell stock? - (reply: 2)
      
1789. Clonogenic assay with agressive cell line - (reply: 4)
      
1790. e coli cell lysis - heterologous gene expression in e coli (reply: 3)
      
1791. Number of cells in 6 wells-plate well - (reply: 1)
      
1792. protein extraction from primary cells - (reply: 1)
      
1793. Extracting proteins from cells - (reply: 1)
      
1794. Minimal amounts of whole cell extracts for Co-IP - (reply: 1)
      
1795. strange dots in cell culture flask?? - U87 cell line culture has strange white dots (reply: 6)
      
1796. cell floating in 96-well plate - (reply: 1)
      
1797. Inducing Adherence in Suspension Cells - (reply: 1)
      
1798. bacterial contamination in cell culture - (reply: 3)
      
1799. THP-1 cell culture - (reply: 1)
      
1800. H9c2 cell contamination? - (reply: 4)
      
1801. Recovery plasmids from dead bacteria - How can i rescue my plasmid from dead cells??? (reply: 4)
      
1802. Inducible expression in human cell line - suggestions for a good system? (reply: 4)
      
1803. transfection in floating cells - (reply: 5)
      
1804. immortal or stable cells - primary human cells to immortal cell line? (reply: 3)
      
1805. SDS-PAGE Cell lysis problems - (reply: 3)
      
1806. Storage of invitrogen top10 cells - (reply: 11)
      
1807. oxidative stress - how to measure oxidative stress in cells (reply: 2)
      
1808. Cells clots forming during preparation mouse splenocytes - (reply: 4)
      
1809. Transwell Cell Migration/Invasion Assay - (reply: 4)
      
1810. neuronal nitric oxide synthase/cell line - (reply: 3)
      
1811. HL 60 cells passage - (reply: 1)
      
1812. Establishing a new cell line - (reply: 1)
      
1813. Isolating Natural Killer cells from whole blood - (reply: 2)
      
1814. Centrifugation of epithelial cells - (reply: 2)
      
1815. cell engineering exam - (reply: 5)
      
1816. Cell detachment: alternative to trypsine/EDTA? - (reply: 3)
      
1817. Cell supplier - (reply: 2)
      
1818. Adherent cells versus suspension cells - how can i convert adherent cell culture to suspension culture (reply: 6)
      
1819. Mir34a positive control cells - (reply: 4)
      
1820. Medium for MDCK cells overexpressing MRP-1 - (reply: 1)
      
1821. lentiviral transdcution of hematopoëtic stem cells - (reply: 2)
      
1822. FACS positive cells in negative controls help - (reply: 1)
      
1823. Protein Removal from cell lysate - leaving RNA intact! (reply: 1)
      
1824. How to separate fungus cells from human lung tissues - (reply: 7)
      
1825. Growing of TOP10 chemically competent cells - (reply: 4)
      
1826. Stable Cell Selection with Neomycin - (reply: 4)
      
1827. endogenous miRNA of cos-7 cell - (reply: 2)
      
1828. Qustion about MDA-MB-157 cells culture - (reply: 4)
      
1829. Folic acid solution for cell culture - (reply: 2)
      
1830. Strange dots around the cell+Photo - (reply: 6)
      
1831. FACS of GFP expressing cells from mice brains - (reply: 1)
      
1832. cells can't attach in the flasks - (reply: 4)
      
1833. Differential expression of 3 adenoviruses in different cell lines. Why? - (reply: 3)
      
1834. Cell fractionation and isolation of nuclei - (reply: 1)
      
1835. Methanol toxicity in cell culture - (reply: 15)
      
1836. Freezing cells after fix & perm and also a BrdU question - (reply: 4)
      
1837. Freezing cells, quick question - (reply: 1)
      
1838. Best place to buy cells - (reply: 2)
      
1839. help on generation of stable cell lines with pBabe vector - pBabe-puro, phoenix Ampho cells (reply: 7)
      
1840. DMSO usage in cell culture - (reply: 9)
      
1841. stopping brief cell treatments - (reply: 2)
      
1842. Phenol red in media - What metabolic products of cells are acidic? (reply: 2)
      
1843. Cell culture container - (reply: 2)
      
1844. cell lysis method - (reply: 1)
      
1845. Dendritic cell immunology - activated naive cells? (reply: 9)
      
1846. cell contamination???? - (reply: 1)
      
1847. counting number of cells - (reply: 6)
      
1848. Stacking of RAW 264.7 cells - (reply: 1)
      
1849. retroviral generation of stable cell line - cells just don't seem to be growing (reply: 3)
      
1850. Lentiviral transduction of B cells - (reply: 7)
      
1851. Lentivirus transduction of spleen cells - (reply: 2)
      
1852. trouble with establishing a stable cell line - (reply: 3)
      
1853. Cell culture conditions and antigen expression - (reply: 1)
      
1854. Phoenix packaging cell and diphtheria toxin - (reply: 3)
      
1855. Gene expression in cell line - (reply: 1)
      
1856. isoflurane and FACS analysis of peripheral blood? - my cells look terrible everytime I use isoflurane to anaesthetize (reply: 5)
      
1857. Growing Up Cells - (reply: 3)
      
1858. protein extraction after cell lysing - (reply: 2)
      
1859. cell line for neurodevelopment - (reply: 2)
      
1860. Difficulty in doing hemocytometer cell count - (reply: 4)
      
1861. No RNA from cell pellet using Qiagen MiniKit!!??!! - (reply: 5)
      
1862. Method for lysing cells for fractionation - (reply: 4)
      
1863. Neuronal cell culture - why use neonatal brains? - (reply: 1)
      
1864. Detect gene in stable cell line, - (reply: 4)
      
1865. staining cells - (reply: 3)
      
1866. pIRESneo for mammalian cells transfection - (reply: 4)
      
1867. Protocol for staining live cells - Staining live cells for Flow (reply: 2)
      
1868. H9C2 - cell line - Need clarification (reply: 1)
      
1869. Cell plating calculation !! - Help needed with Cell plating calculation !! (reply: 2)
      
1870. Cell lines to study epigenetics - (reply: 2)
      
1871. How to transfect two plasmids in a cell - (reply: 9)
      
1872. Cells dying before i can get them to in vitro assay - (reply: 1)
      
1873. Strange bands in western of RPE-1 cells - (reply: 1)
      
1874. Semisolid Medium For HL 60 cells - (reply: 4)
      
1875. Cell culture contamination (do not know what it was) - (reply: 1)
      
1876. questions about cell lysis - (reply: 7)
      
1877. Gateway Cloning Problem - Lawn of E. Coli Cells - (reply: 4)
      
1878. How to homogenize cell without disruption of nuclei? - (reply: 2)
      
1879. Harvesting cells for flow cytometry - (reply: 10)
      
1880. culture C6/36 insect cell lines - (reply: 1)
      
1881. seeding cell densities - seeding cells at same density (reply: 1)
      
1882. cell culture best practices - virus immortalized and non-immortalized cells (reply: 1)
      
1883. Culturing QT6 cell line - Ideal culture conditions (reply: 2)
      
1884. Calculating MOI for luciferase-transducted cells. - Would the sensitivity of a fluorescent microscope be enough to see the (reply: 3)
      
1885. Picking epithelial, endothelial and neural cell lines - Please suggest best models (reply: 2)
      
1886. How to fix suspencion cells for DAPI detecting under the microscope? - (reply: 6)
      
1887. primary lung epithelial cells - (reply: 1)
      
1888. Generating double stable cell line - (reply: 3)
      
1889. HACAT cells - very low cell viability after thawing cells (reply: 18)
      
1890. cell line for luciferase assay - How to choose the best cell line? (reply: 2)
      
1891. My cells suddenly die !!! - Please help me ! why is this ? (reply: 11)
      
1892. database for mutations in cancer cell lines? - (reply: 2)
      
1893. Sonication Problems, Branson 450, LNCaP cells - (reply: 4)
      
1894. ? Definition of Passage for suspension cells - (reply: 1)
      
1895. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
      
1896. different staining intensity - fixed permeabilized cells and non-fixed cells (reply: 2)
      
1897. Chip problem: antibody or cell number?? - (reply: 6)
      
1898. stable cell line - (reply: 1)
      
1899. microbial cell viability - any recommendations - assays (reply: 1)
      
1900. SF9 cells not adhering - (reply: 10)
      
1901. HeLa cells - Growth of thawed HeLa cells (reply: 7)
      
1902. Dendritic Cells and Flow Cytometry - (reply: 11)
      
1903. direct cell lysate - (reply: 8)
      
1904. How long can I wait after seeing HeLa cells till transfection - will it affect my Efficiency (reply: 6)
      
1905. Culturing/Mounting of polarised cells for confocal microscopy - (reply: 1)
      
1906. Intestinal cell culture: Protocol required - (reply: 1)
      
1907. Carbon di oxide free cell culture - (reply: 1)
      
1908. surface staining of THP-1 cell - (reply: 2)
      
1909. How to fix GFP expressing cell to preserve GFP signal in immunofluorescent stain - (reply: 3)
      
1910. Cell Wall Lysis For Dummies - (reply: 4)
      
1911. Tissue & Cell Fixation solutions - (reply: 3)
      
1912. MSP: No PCR product for one cell line but yes for the other cell lines - (reply: 3)
      
1913. Treating cells with chemicals and how to dilute into medium - (reply: 5)
      
1914. T lymphocyte ChIPs are not working... - while other cells are! *grumble grumble* (reply: 6)
      
1915. Using Real Time PCR for Cell Viability - Any tips? (reply: 6)
      
1916. Has anyone used COS-7 cells for retina? - (reply: 1)
      
1917. How to perform a growth curve? - i need to compare 3 cell lines for growth (reply: 2)
      
1918. siRNA concentration in divinding-growing cells - (reply: 2)
      
1919. siRNA transfection of RS4-11 and MV-4-11 Cells - (reply: 1)
      
1920. cell culture guideline from abcam - (reply: 5)
      
1921. Whole cell PCR - DNA purification without using proteinase K? (reply: 8)
      
1922. problems with detecting protein expression - protein expression in cell (reply: 4)
      
1923. freeze PBMC and cell sorting? - (reply: 1)
      
1924. Control vehicle treatment in cells - (reply: 2)
      
1925. Cancer cell culture exploded onto my face - (reply: 8)
      
1926. FACS analysis of GFP expressing cells - Fix or not - (reply: 10)
      
1927. HELP URGENT! FLUID MOSAIC / CELL MEMBRANE - (reply: 2)
      
1928. Sticky Insect cell lysate blocked Ni_NTA column - (reply: 2)
      
1929. Heterogeneous methylation in a same cell line - (reply: 1)
      
1930. siRNA cell function examples - (reply: 1)
      
1931. Good comprehensive resource for maintaining immortal cell lines? - (reply: 1)
      
1932. DH5a comp cells - (reply: 5)
      
1933. Trypsinized cells after fixation - (reply: 3)
      
1934. Detaching Hela cells - (reply: 3)
      
1935. help! Truncated protein cannot expressed in mammalian cells? - (reply: 6)
      
1936. cell cycle softawe - (reply: 3)
      
1937. How to maintain cells at a specific apoptosis stage - (reply: 12)
      
1938. Which cell line produce cytokine? - (reply: 13)
      
1939. Cell Viability - (reply: 1)
      
1940. Shipping of cryopreseved cell - (reply: 15)
      
1941. To what extent competent cells be thawed before transformation? - (reply: 4)
      
1942. Lipofection transfection of HEK293 cells unsuccessful - (reply: 10)
      
1943. Storing HEK293 cells at -80C - (reply: 5)
      
1944. How to control cloned cDNA expression level in cells? - (reply: 8)
      
1945. RNA isolation following apoptotic cell labeling - (reply: 3)
      
1946. Why are my E. coli cells not pellet-able? - when expressing a chimeric recombinant protein (reply: 6)
      
1947. Is there an optimal incubation time for cell lysis? - (reply: 7)
      
1948. The way to count adherent cells in 96 well - (reply: 3)
      
1949. lysing cells in LSB - (reply: 2)
      
1950. Using NIH3T3 cells of look at MAPK activation by Ras - (reply: 1)
      
1951. Staining for neural crest derived cells?? - (reply: 2)
      
1952. Dendritic_ cells - (reply: 3)
      
1953. problem to stain just one cell line in coculture - (reply: 4)
      
1954. Culturing stromal cells - (reply: 2)
      
1955. A549 cells - (reply: 4)
      
1956. Chikungunya Virus Culture - Chikungunya Virus culture in mammalian cells form serum sample of huma (reply: 2)
      
1957. Protein extraction from cell culture media - (reply: 9)
      
1958. Bovine cell line for transfection... - Transfection friendly cell line for localization studies... (reply: 1)
      
1959. Has anyone had experience with GM2163 (dam-) cells? - the enzyme BclI is blocked by dam (reply: 1)
      
1960. yeast gene deletion - Could I delete an essential gene from haploid cells? (reply: 8)
      
1961. can we prepare Copmetent cell by calicum cloride with 0.09 OD - Cp cells with 0.09 OD (reply: 3)
      
1962. electroporation of HL60 cells - (reply: 1)
      
1963. Intestinal epithelial cell culture - (reply: 4)
      
1964. SF9 cell transfection using lipofectamine?? - and is CMV active in this cells? (reply: 3)
      
1965. Easiest cell lysis protocol - no interest in preserving proteines (reply: 4)
      
1966. passage number for cell line - (reply: 7)
      
1967. MOCK INFECTED CELLS - (reply: 4)
      
1968. cuboidal cells - (reply: 1)
      
1969. Cell count using a hemocytometer - (reply: 4)
      
1970. Buffers that are good for cell culture. - need buffering capacity around pH 5-7 (reply: 3)
      
1971. what is mock cell line? - (reply: 4)
      
1972. 293Flp cell transfection - Passaging cells 4-6 hours after lipofectamine trasnsfection (reply: 3)
      
1973. How to seed the cells evenly in 6 wells plate - (reply: 13)
      
1974. knockout mouse with increase cell size - (reply: 3)
      
1975. Does UV cause DNA fragmentation? - how long do I need to put cells under UV to see fragmentation? (reply: 12)
      
1976. Paper on energy requirement of cells - (reply: 2)
      
1977. galactose induction of yeast cells - (reply: 2)
      
1978. making competent E coli DH5a cells - (reply: 6)
      
1979. How could you explain these results? - cancer cells are weird (reply: 1)
      
1980. strange ES cells contamination - please help!!! (reply: 1)
      
1981. Hek 293 cell line with tetra/doxycycline induction - how to lower expression level? (reply: 2)
      
1982. How to culture PC3 cell line - (reply: 3)
      

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