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Cell culture Related Discussions

1. Correlation between microorganism division rate and cell dimorphism - (reply: 1)
   
2. Help! Cell contamination ? - (reply: 1)
   
3. cell culture medium - (reply: 1)
   
4. Does the volume of buffer you resuspend a pellet in affect cell yield? - (reply: 1)
   
5. Inhibition of SARS-CoV-2 in Vero cell cultures by peptide-conjugated morpholino- - (reply: 8)
   
6. Generating a cell line using sleeping beauty transposase and a gene that contain - (reply: 1)
   
7. Adherent cells detach themselve from petri dish (problems in cell culture) - (reply: 6)
   
8. HELP: Validation of a polyclonal knock out (KO) cell line when you dont have a g - (reply: 6)
   
9. expressing bacteriophage in mammalian cells - (reply: 5)
   
10. cell free DNA (cfDNA) - (reply: 1)
    
11. Contamination of cell culture - Weird stringy thing in cell culture flasks - (reply: 2)
    
12. How to calculate cell viability percentage from the absorbance in LDH assay? - (reply: 2)
    
13. trypan blue assay - (reply: 2)
    
14. what is mean by Mock cell? - (reply: 1)
    
15. What is going on with these cells? - (reply: 3)
    
16. Can glucose-6-phosphate enter human cells? - (reply: 2)
    
17. Electrophoresis of proteins from cells - (reply: 9)
    
18. Issue about reusing cells - (reply: 1)
    
19. cell line authentication - (reply: 1)
    
20. MNFS60 cells are dying. - (reply: 2)
    
21. De-clumping cells - (reply: 1)
    
22. Cell Biology: Transcription - (reply: 2)
    
23. Why does cell division, as asexual reproduction, perpetuate life of unicellulars - (reply: 4)
    
24. Releasing cell associated virus - (reply: 14)
    
25. Two distinct populations observed on a homogenous cells - (reply: 1)
    
26. Help novice with question about cell concentration in batch culture - (reply: 3)
    
27. Cell counting - (reply: 12)
    
28. HeLa cells dangerous? - (reply: 4)
    
29. black spots are found in cells and medium - (reply: 1)
    
30. Unidentified geometric objects in cell culture - (reply: 3)
    
31. Cell Membranes as an active barrier - (reply: 1)
    
32. RNA degradation from cells embedded in gels - (reply: 4)
    
33. anti-HA immunofluorescence on HEK 293 cells - (reply: 5)
    
34. restoring melanocyte stem cells in gray hair - (reply: 10)
    
35. Normalise cell count for protein analysis - (reply: 3)
    
36. Blockage of cadherin bindings of MCF-10A cells without harming hydrogels and cal - (reply: 2)
    
37. 293T cells not able to attach well after passage - (reply: 1)
    
38. Decrease of total cells in a stirred bioreactor cultivation? - (reply: 1)
    
39. Reorganization of liquid nitrogen cell stocks - (reply: 1)
    
40. Should Lineage marker cocktail be used in cell lines to remove lineage specific - (reply: 1)
    
41. How to make normal cells cancerous - (reply: 1)
    
42. 293T cells unable to revive - (reply: 5)
    
43. Counting number of cells - (reply: 1)
    
44. What is the source of contamination in my cell culture? - (reply: 2)
    
45. Stem cell imaging without a need for fluorescence: win a microscope (deadline No - (reply: 1)
    
46. prepare competent cells from existing competent cells - (reply: 2)
    
47. slimy cell paste of recombinant e.coli - (reply: 3)
    
48. Cell Counting - (reply: 1)
    
49. Cell cycle - (reply: 2)
    
50. How long do you reckon a cell pellet can survive before it must be resuspended? - (reply: 1)
    
51. ¿must treatment be aplied 24h post seed the cells? - (reply: 1)
    
52. Question about transfecting siRNA into cells - (reply: 1)
    
53. Mammalian Cell Lysis - (reply: 1)
    
54. 293T cells for lentivirus production - (reply: 1)
    
55. Counting and seeding cells?]. - (reply: 1)
    
56. propagating commercial chemically competent cells - (reply: 2)
    
57. CRISPR-Cas9 / insert a gene in cell line genome - (reply: 1)
    
58. Beginner Cell and Tissue, and Molecular Biology methods reading list - (reply: 1)
    
59. how to present Michaelis-Menten equation in cell designer - (reply: 1)
    
60. TCDD (2,3,7,8-​Tetrachlorodibenzo-p-dioxin solution) cell line treatment - (reply: 1)
    
61. cell invasion assay - (reply: 1)
    
62. Help with the Red Blood Cell Lysis - (reply: 1)
    
63. gene expression in cultured cell lines - (reply: 1)
    
64. Splitting cells without centrifugation - (reply: 4)
    
65. Are HEK293 cells a positive control for wild type p53 expression? - (reply: 1)
    
66. Culturing LoVo cells - (reply: 1)
    
67. Black dots contaminant in stable cell lines - antibiotics - (reply: 1)
    
68. cell viability assay for caco-2 and pbmc - (reply: 1)
    
69. Subculturing colonies to agar - (reply: 2)
    
70. 3T3 L1 cell culture - (reply: 1)
    
71. Why my bacteria cells look weird in DAPI fluorescence? - (reply: 2)
    
72. B & T cell activation in autoimmune disorders - (reply: 1)
    
73. lentiviral transduction in BLCL cells - (reply: 3)
    
74. Synchronization of HEK293T cells for IP - (reply: 2)
    
75. Alternate cell line not giving same results as first - (reply: 1)
    
76. Cell volume and reciprocal cell volume? Whaaat? - (reply: 1)
    
77. How to detect specific-protein producing cells? - (reply: 8)
    
78. Who came up with 50-100 IU/mL Antibiotic working concentration in cell culture? - (reply: 2)
    
79. Help! Antibiotic and Serum Concentration in Cell Culture Media - (reply: 1)
    
80. Large-scale cell growth and protein expression - (reply: 1)
    
81. is cell signaling difficult? - (reply: 3)
    
82. What would be the shortest and optimal method of extracting human cells for PCR? - (reply: 2)
    
83. The adipose cell culturing - (reply: 2)
    
84. Recommendation of cell signaling inhibitors - (reply: 2)
    
85. Recovering cells from ancient sample - (reply: 3)
    
86. Microinjection of cells into the lumen of spheroids ? - (reply: 1)
    
87. Human cell membrane protein Western blot! - (reply: 2)
    
88. Cell Line confluence level - (reply: 1)
    
89. too much cell density can influence adhesion to culture plate - (reply: 1)
    
90. Shipment conditions for cultured cells - (reply: 3)
    
91. pIKK antibody for western blot (RAW 264.7 cells) - any recommendations? - (reply: 1)
    
92. E-Cadherin Expression in OAW-42 Cells - (reply: 1)
    
93. Why HepG2 cells looked like that? - (reply: 2)
    
94. Trouble transfecting HEK 293 T and HUH 7 cells - (reply: 1)
    
95. 3T3-L1 cell contamination - (reply: 3)
    
96. Will lipofection of DNA work in post mitotic cells - (reply: 1)
    
97. Cell death determination - (reply: 2)
    
98. Culture Tubes for CHO cells - (reply: 1)
    
99. SHSH5Y cells - (reply: 1)
    
100. Is the mycoplasma that infects cell culture dangerous to humans? - (reply: 1)
     
101. Distinguishing two populations of cells by TEM - (reply: 4)
     
102. transfection of suspended CHO cells - (reply: 2)
     
103. using IC50 to determine dosing of inhibitor in cell culture - (reply: 2)
     
104. Software for automated cell counting? - (reply: 2)
     
105. why is my cells dying in the centre of the wells after transfections - (reply: 1)
     
106. How to establish drug-resistant cell lines? - (reply: 1)
     
107. Are normal or immortalized human epithelial cells clonogenic? - (reply: 1)
     
108. BJAB cells: does anyone have a specific protocol for transfection? - (reply: 1)
     
109. Fibroblast Cell Culture Issue - Cells grouping together and cell clusters observ - (reply: 5)
     
110. pUC and pSC101 vectors in the same cell - (reply: 3)
     
111. Jurkat cell - normal receptor/gene expressivity - (reply: 1)
     
112. Can propidium iodide be used on WBC nuclei with cell membrane removed - (reply: 1)
     
113. Inactivating effects on immune cells caused by mutation in the gene encoding the - (reply: 2)
     
114. Is cultured cell reuse possible? - (reply: 1)
     
115. Count cell bactria using haemacytometer - (reply: 1)
     
116. Help with HUVEC cell transfection - (reply: 3)
     
117. Cell clumps or contamination? Please help! - (reply: 1)
     
118. How to clean up cell fragments before cell sorting - (reply: 1)
     
119. Is any other method to maintain without changing media, or store cell culture fo - (reply: 2)
     
120. MDA-kb2 cells - problem with luciferase expression - (reply: 6)
     
121. Cell lysis method to get "intact" nuclear protein complex for CO-IP? - (reply: 1)
     
122. Abnormality in PMA treated THP1 cells - (reply: 1)
     
123. Preferred assay for measuring cytotoxicity or cell proliferation - (reply: 2)
     
124. Cell lysis - (reply: 1)
     
125. Can foam created when resuspending a cell pellet cause cell demage - (reply: 1)
     
126. Distribution of cell receptors on the cell surface - (reply: 3)
     
127. MTT Cell Treatment - (reply: 1)
     
128. cells behaving differently - (reply: 4)
     
129. Cell lines behaving differently to drugs - (reply: 2)
     
130. How to achieve a uniform monolayer with HepG2 cells, seeded in 24 welll plate fo - (reply: 1)
     
131. Efficient lysis of E.coli cells - (reply: 4)
     
132. Alternative competent cells for QuikChange kit - (reply: 2)
     
133. unknown bodies in cell culture - (reply: 1)
     
134. Unbroken cell - (reply: 1)
     
135. how to confirm lentiviral integration in a target cell line - (reply: 1)
     
136. Invasion assay: analysis question: round cells - (reply: 2)
     
137. Measuring ammonia in cell lysates - (reply: 1)
     
138. Methanol oor PFA to fix cells for nuclear protien analysis by immunofluorescence - (reply: 4)
     
139. What happens when trypsin is added to cells? - (reply: 1)
     
140. Selective evolution of cells for cancer - (reply: 4)
     
141. cell line - (reply: 3)
     
142. I want know what companies are producing Biosimilar cell line - (reply: 1)
     
143. contamination in HepG2 cell culture - (reply: 1)
     
144. Which neuroblastoma cell lines grow the longest axons - (reply: 1)
     
145. Help with Question for Cell Bio Take Home Exam - (reply: 2)
     
146. leukemic cell lines 697 - (reply: 1)
     
147. where do you get cells needed for your research - (reply: 1)
     
148. PCR with mammalian cells as template - (reply: 1)
     
149. Dislodge cell in 96well plate - (reply: 1)
     
150. The right lentivirus plasmid for overexpression in human cells - (reply: 7)
     
151. cell counting - (reply: 2)
     
152. What do the glioma cell line prefixes LN and U stand for ? - (reply: 1)
     
153. Trypan Blue Staining - (reply: 1)
     
154. colorectal cell culture - (reply: 2)
     
155. Easy to transfect primary cell line? - (reply: 1)
     
156. Sample prep of cells for FACS analysis and running WB - (reply: 4)
     
157. Functional states of the same cell versus Different cell types - (reply: 2)
     
158. How long after transfection or dox induction of stable cell line before performi - (reply: 1)
     
159. transfection of cells with two fluorescent reporters - (reply: 1)
     
160. Stably transfected cells start dying - (reply: 6)
     
161. U251 and U87 cells - (reply: 1)
     
162. is -40 freezer enough for cell freezing? - (reply: 1)
     
163. Experiment on nutrient surplus on Jurkat cell culture - (reply: 2)
     
164. Help with HeLa cell cultivation - (reply: 5)
     
165. skin lesion and low donor cell frequency in bone marrow transfer - (reply: 7)
     
166. cell division rates - (reply: 6)
     
167. Black dots-ghost cells or contamination? Please help - (reply: 6)
     
168. Where do you purchase T cell hybridomas - (reply: 5)
     
169. NK Cell Culture Selection and Expansion from PBMC - (reply: 2)
     
170. 3D Cell Culture Matrix made of jelly / gelatin - (reply: 2)
     
171. Cloning purified PCR product into cells - (reply: 5)
     
172. Help ID fluoescently-labelled cells in the gut very much appreciated! - (reply: 2)
     
173. Mention of Transgene Insertion into Cell Lines By Transfecting with cDNA - (reply: 2)
     
174. Refreeze thawed cells? - (reply: 3)
     
175. How can an animal cell obtain needed glucose when glucose is lacking in the diet - (reply: 2)
     
176. Suppose a microorganism invades a cell, causing damage to ATP synthase. How woul - (reply: 3)
     
177. How to capture live cells under microscope - (reply: 3)
     
178. Identification of cells and tissue - (reply: 2)
     
179. Research topic related to jurkat cell line - (reply: 3)
     
180. What DCH5a cells are? / pCAMBIA1301 isolation from DHC5a cells - (reply: 3)
     
181. When is it suitable for using NIH3T3 cells to validate cancer genes? - (reply: 1)
     
182. Assays from very few number of cells - (reply: 1)
     
183. What I have to do If my active compound showing toxicity in normal cells? - (reply: 1)
     
184. Passaging cells - (reply: 1)
     
185. Problem in mouse embryonic stem cell culture - (reply: 4)
     
186. Is there always a presence of activated T cells in a healthy host? - (reply: 1)
     
187. Bacterial contamination in cell culture - (reply: 3)
     
188. Cell culture contamination - (reply: 1)
     
189. Contamination or dead cells? - (reply: 1)
     
190. How to choose cell line - (reply: 4)
     
191. Urea effect on mice spleen cells - (reply: 3)
     
192. Urea effect on mice spleen cells - (reply: 1)
     
193. Trypan Blue if cells cultured in 96 well plate - (reply: 2)
     
194. Best cryovials for liquid nitrogen storage of cells? - (reply: 2)
     
195. Review articles on all types of mammalian cell culture - Any suggestion? - (reply: 1)
     
196. 2 types of cell lines for RNA extraction - (reply: 1)
     
197. live cell culture transport in 6 well plate - (reply: 6)
     
198. Stem Cell Culture Theory - (reply: 4)
     
199. Selectively arresting a subpopulation of biotinylated yeast cells. - (reply: 4)
     
200. cell viability with PI staining - (reply: 2)
     
201. trypsin digestion of 2D-PAGE protein spot - (reply: 2)
     
202. Sonication of cells in ultrasound bath, without probe - (reply: 3)
     
203. cell fractionation and microscopy - (reply: 3)
     
204. Cell Cycle Analysis - crucial to keep cells cold after ethanol fixation, and aft - (reply: 1)
     
205. cell lysis problem - (reply: 2)
     
206. Made a mistake and fixed cells with 30% ethanol for cell cycle analysis. Can I s - (reply: 1)
     
207. Thawing cells - (reply: 2)
     
208. the same construct, two stable cell clones with different protein size - (reply: 2)
     
209. Determine DNA sequence expression in cell line - (reply: 1)
     
210. Harvesting cells - (reply: 3)
     
211. Cell counters - Beckman Coulter Z1 counter - (reply: 1)
     
212. Competent cells with Hanahan's method - (reply: 2)
     
213. problem with cell synchronization - (reply: 4)
     
214. can anyone tell me what kind of cell this is? - (reply: 3)
     
215. Transfection of SH-SY5Y cells - (reply: 4)
     
216. Protocol for isolating cells from Subcutaneous Tumors ? - (reply: 1)
     
217. Hybridoma cells contaminated with large round cells! - (reply: 1)
     
218. protocol for cell syncronization using colchicine - (reply: 2)
     
219. PBMC cell stimulation? - (reply: 1)
     
220. Caco-2 cells with unidentified contaminant - (reply: 2)
     
221. MIN6 Cell Culture Contamination Risk - (reply: 1)
     
222. Concentrating bacterial cells - (reply: 2)
     
223. Would preserved cells for flow cytometry make your life easier? - (reply: 1)
     
224. measure of cell size - (reply: 1)
     
225. Transfecting cells for cell cycle analysis, how to deal with cell density? - (reply: 1)
     
226. Splitting Cells that are shaped as small spheres - (reply: 2)
     
227. Cells counting problem. - (reply: 2)
     
228. Cell counts for Clonogenic and MTT assay - (reply: 3)
     
229. Do I need to use untreated TC flask for suspension cell culture - (reply: 5)
     
230. Cell cycle determination - (reply: 1)
     
231. After centrifuge in serum-free media, forming cell sheet, instead of pellet in t - (reply: 2)
     
232. Can cells be exposed to nanoparticles with ethanol as solvent??? - (reply: 2)
     
233. Question about growth media for A20 Cell Line - (reply: 1)
     
234. NSO cell culture - (reply: 1)
     
235. Some queries regarding A549 cell culture - (reply: 1)
     
236. Has anyone experience in isolating single-cell clones of CHO transfected cell li - (reply: 4)
     
237. how to transport stem cells in suspension? - (reply: 2)
     
238. animal cell culture - (reply: 1)
     
239. help with contamination of cell culture - (reply: 2)
     
240. Methanol concentration for cells? - (reply: 4)
     
241. What happened to my cell? (Colony formation) - (reply: 7)
     
242. problem of cell lysate - (reply: 3)
     
243. Cell lysis and DNA quantification - (reply: 1)
     
244. Best western loading control for comparing different cell lines - (reply: 2)
     
245. B cell line - cell colour change and visible cell death - (reply: 1)
     
246. Keeping apoptotic/detached cells for WB? - (reply: 3)
     
247. Effective inhibitor of cell proliferation - (reply: 4)
     
248. Lentivirus transfection using RAW 264.7 cells - (reply: 2)
     
249. Neural Stem Cells; does it mean adult or embryonic? - (reply: 1)
     
250. Cell culture contamination or not? - (reply: 1)
     
251. orthotopic mouse model of U251 cell line - (reply: 4)
     
252. How to release the bacterial cells from filter paper? - (reply: 4)
     
253. Cell adhesion/detachment - (reply: 1)
     
254. methylation observation in normal cell line - (reply: 1)
     
255. Stem cells created by dipping researchers into coffee - (reply: 2)
     
256. Freezing yeast cells - (reply: 4)
     
257. codon optimization for CHO cells - (reply: 2)
     
258. FITC conjugated phalloidin staining of cells on microcarrier beads - (reply: 1)
     
259. chk effect of cell line genes on normal dna - (reply: 1)
     
260. Would someone help me discuss what work can I do with laryngeal cancer cell hep2 - (reply: 12)
     
261. DAPI stainged dots only in cells transfected with Fugene6 - (reply: 5)
     
262. 293T cell transfection troubles - Lipofecamine 2000 - (reply: 3)
     
263. Can I make my own electrocompetent Stbl4 cells? - (reply: 2)
     
264. CHIP on frozen cell pellet - (reply: 1)
     
265. UV Light benefits and costs on Cells - (reply: 2)
     
266. Adapting a Drosophila cell culture to Zebrafish for subsequent MACS-sorting - (reply: 3)
     
267. mouse embryonic stem cell culture on gelatine coated plates - (reply: 1)
     
268. easiest cell lines to use - (reply: 4)
     
269. Jurkat cell - (reply: 4)
     
270. Mesenchymal stem cells Expressing BDNF - (reply: 5)
     
271. PI staining cell cycle analysis - (reply: 1)
     
272. Protein Purification from mammalian cells - (reply: 1)
     
273. How to identify CD8 T cell receptor for a particular antigen - (reply: 2)
     
274. How to inactivate H1N1 virus in Cell culture supernatant ? - (reply: 3)
     
275. HaCaT cell line - not responding to inflammatory stimuli such as LPS/TNF-alpha - (reply: 4)
     
276. Homogenous Cell Lysates - (reply: 1)
     
277. Does anyone irradiate their cells? - (reply: 3)
     
278. need help for transfection to synchronized cells - (reply: 4)
     
279. If MCF-7 cell line can expresses Her-2 gene? - (reply: 5)
     
280. What should I suspend my cells in prior to cytometry? - (reply: 2)
     
281. Checking for efficieny competent cells - (reply: 1)
     
282. Video on cell culture technique (broad brush) - (reply: 2)
     
283. Setting up two drug kill curve for selection of stable cells - (reply: 1)
     
284. No expression in stable transfected cell line - (reply: 2)
     
285. Protocol for flow cytometry on mononuclear cells from mouse blood - (reply: 1)
     
286. Cell line contamination? - (reply: 5)
     
287. Inducing apoptosis by puromycin selection: toxic to the selected cells? - (reply: 1)
     
288. Measuring cell proliferation from daily aliquots - (reply: 1)
     
289. Cell culture of MYC immortalised HDFa - need advice - (reply: 1)
     
290. Phoenix Amphoteric cell line to package lentivirus? - (reply: 6)
     
291. miR30 based shRNA in mouse cells - (reply: 3)
     
292. Cells are unhealthy - (reply: 2)
     
293. Harvesting cells - (reply: 12)
     
294. Dead cells after transfection - (reply: 9)
     
295. preparation of cells for FACS analysis after transfection - (reply: 4)
     
296. simultaneous knock down of two genes in a single cell line - (reply: 1)
     
297. CoIP from cell lysate mix - (reply: 5)
     
298. selective pressure on cells - (reply: 2)
     
299. Conjugation of Ab to magnetic beads to isolate T cells from Spleen - (reply: 2)
     
300. cell culture and biopsy - (reply: 1)
     
301. Tiny black dots inside cell vacuoles - (reply: 1)
     
302. I always have a cell depris floating in the supernatent of MDCK cells - (reply: 3)
     
303. Is Trifluoroacetic acid toxic for cells? - (reply: 5)
     
304. mycoplasma testing - (reply: 1)
     
305. Treating suspension cells - (reply: 2)
     
306. H9c2 cells..please help - (reply: 2)
     
307. loading control issue in blot with H9c2 cell protein, pleeeeeasee help - (reply: 2)
     
308. How can i seed 2000 cell /well? (Using 200 microliter media and 96 plate, and as - (reply: 1)
     
309. Establishing human neural stem cells - (reply: 3)
     
310. Immunofluorescence Staining Problem of Mitotic Cells - (reply: 2)
     
311. Cell Adhesion and Motility on Fibronectin vs Collagen - (reply: 1)
     
312. FCS in Serum free medium for better Cell Adhesion? - (reply: 1)
     
313. Possible to sort cells from formalin? - (reply: 1)
     
314. 293s vs HEK/293 cells - (reply: 2)
     
315. Cell culture medium contamination? - (reply: 1)
     
316. What do sick 293t cells look like? - (reply: 3)
     
317. H2DCFDA and fixation of cells before flow cytometry analysis - (reply: 4)
     
318. Decreasing cell division - (reply: 1)
     
319. Can I use methanol fixation to fix cells before proteomic analysis? - (reply: 1)
     
320. Is it generally acceptable to ask another lab for cell line/plasmid/etc - (reply: 4)
     
321. Do S2 cells normally have small 1-2um objects floating in culture? - (reply: 1)
     
322. Problem in Cell Viewing - (reply: 3)
     
323. How to view the encapsulated cells under microscope? - (reply: 5)
     
324. Poor data quality using cultured cell in aCGH - (reply: 10)
     
325. Immunohistochemical estrogen/progesterone receptor staining for CELLS - (reply: 1)
     
326. Totipotent Stem Cell From Molecular Components - (reply: 5)
     
327. How to count clumpy epithelial cells? - (reply: 5)
     
328. Why cells are floating after incubation in EBSS?? - (reply: 1)
     
329. purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
     
330. Live cell sort DNA stain permeabilization - (reply: 1)
     
331. Problem in bacterial cell culture - (reply: 12)
     
332. Persistent contamination in cell culture - (reply: 9)
     
333. PE and FITC stain after fixing cells with 4% PFA - (reply: 3)
     
334. Adult stem cells - (reply: 3)
     
335. Centrifuge stopped working while my blood samples were incubated in Cell lysis s - (reply: 1)
     
336. Ways to Quickly Identify Antibiotic Resistance in Bacterial Cells (Non-tradition - (reply: 12)
     
337. freezing competent cells without dry ice or liquid N - (reply: 3)
     
338. Suspension cell line (RPMI) adhere to flask-WHY? - (reply: 1)
     
339. Rapamycin Concentration for Cell Culture - (reply: 1)
     
340. how to calculate cells and plate - (reply: 2)
     
341. yeast conatamination in primary cells - (reply: 4)
     
342. Maltodextrin will kill all the bacterial cells? - (reply: 5)
     
343. Keratinocyte Culture: Cells detach - (reply: 7)
     
344. Measure growth of plant cell culture - (reply: 1)
     
345. Cell Lysate Western Blot Little or No Signal - (reply: 7)
     
346. Cell line identity (HEK293-E) - (reply: 1)
     
347. How do I calculate the enery absorved by cells form a UV light - (reply: 3)
     
348. Immunocytochemistry - Washing away PC12 cells - (reply: 2)
     
349. Protein secretion wtih pFuse expression system in 293T cells - (reply: 2)
     
350. Total Protein in COS-7 Cells - (reply: 1)
     
351. protein extraction from cell nucleus - (reply: 2)
     
352. Which gauge for proper shear force on certain cell dimension - (reply: 1)
     
353. Mammalian Cell Lysis Buffer Triton-X100 Content - (reply: 6)
     
354. DNA from frozed cells??? - (reply: 2)
     
355. SH-SY5Y stable cell transfection and RA differentiation - (reply: 1)
     
356. Tumor cell analysis - (reply: 2)
     
357. What can I do with my MCF7 cells? - (reply: 1)
     
358. Evolution of Living Cells from Non-living Organic Matter - (reply: 3)
     
359. cell lines - (reply: 5)
     
360. M2 antibody background in Xenopus cell extracts - (reply: 6)
     
361. Asking for guidelines to start L6 cell line culturing - (reply: 1)
     
362. how to separate organelles from mammalian cells - (reply: 1)
     
363. Cell culture contamination ? - (reply: 5)
     
364. How to coat 96 well plate with poly-L-Lysine for cell culture use? - (reply: 2)
     
365. Can serum free medium inactivate trypsin? - (reply: 1)
     
366. Is Protein A present on the Staphylococcus saprophyticus cell wall too? - (reply: 2)
     
367. Protein bandshift in western blot of cell lysate vs immunoprecipitation eluent - (reply: 4)
     
368. red dots in cell culture line? - (reply: 3)
     
369. Integrity of cell monolayer - (reply: 1)
     
370. problem with COV318 ovarian cancer cell line - (reply: 4)
     
371. Expansion of commercial competent bacterial cell lines? - (reply: 2)
     
372. atom replacement in human cells - (reply: 2)
     
373. What stage of growth are the cells I counted? - (reply: 4)
     
374. HeLa cell culture - (reply: 6)
     
375. Lambda Phosphatase Treatment of Cell Lysate - (reply: 1)
     
376. Problem in Determination of Total Bacterial Cells - (reply: 13)
     
377. Preparing a cell culture stock to plate out at 7x10^5 per well? - (reply: 1)
     
378. Cell culturing tips - (reply: 2)
     
379. SAOS.2 Cell detachment without using Trypsin. - (reply: 3)
     
380. Normal cells or contamination?! - (reply: 4)
     
381. H2DCFDA for MDCK cells - (reply: 2)
     
382. immortalized human neuronal cells - (reply: 4)
     
383. LNCaP Cell Clusters - (reply: 2)
     
384. Freezing viable cells in 96-well format? - (reply: 3)
     
385. question about sequential transfection several plasmids in 293T and COS7 cells - (reply: 1)
     
386. unidentified objects in my cells, no staining.. what are they? - (reply: 2)
     
387. Does fixation and permeabilization of cells affect their oxidative stress levels - (reply: 1)
     
388. HBE cell culture CRL-2079 MEDIA question - (reply: 1)
     
389. Cells dying in culture after 24 hours - (reply: 6)
     
390. SH-SY5Y cells: basics advice - (reply: 1)
     
391. Cell clumping: dead cells? - (reply: 6)
     
392. What is your favorite method for making chemically competent E.coli cells? - (reply: 7)
     
393. growing cometent cells - (reply: 1)
     
394. Where can I purchase HepG2.2.15 cell line - (reply: 1)
     
395. Mycoplasma detection with ATCC kit - (reply: 1)
     
396. RAW 264.7 cell line - (reply: 1)
     
397. Mycoplasma infected cell line clean-up - (reply: 1)
     
398. Rheumatoid Factor (RF) producing cell line? - (reply: 1)
     
399. Weighing harvested cells - (reply: 1)
     
400. Temperature Variation on Cell Growth Experiment - (reply: 1)
     
401. Morphology of cultured cells - (reply: 5)
     
402. Growing GH teratocarcinoma cell line- help needed - (reply: 2)
     
403. Cells Detach After Transfection - (reply: 1)
     
404. How to determine the location of integrated DNA in stable cell lines - (reply: 2)
     
405. No reduction in protein expr after 2nd passage of transfected cells - (reply: 3)
     
406. When cell media becomes "used up", it acidifies - (reply: 3)
     
407. Cell cycle analysis for SubG1 fraction - (reply: 3)
     
408. Clone selection for scrambled shRNA transfected cells ? - (reply: 2)
     
409. Cytokines feeding cell culture - (reply: 1)
     
410. Experience with U-937 cell line - (reply: 3)
     
411. Cell culture contamination issue - unable to identify the contamination - (reply: 1)
     
412. cell confluency in IF - (reply: 2)
     
413. Protein purification for adherent mammalian cells - (reply: 2)
     
414. Antibody production in Expi293 cells - (reply: 2)
     
415. death of transfected cells after first passage in G418 medium - (reply: 1)
     
416. clonogenic assay for shRNA transfected cells - (reply: 3)
     
417. Cell lysis in chromosome conformation capture - (reply: 2)
     
418. Relationship between cell lines and cancer research (IHC) - (reply: 4)
     
419. Serum starvation - something similar for serum-free kept cells ? - (reply: 2)
     
420. Sodium bicarbonate addition to GMEM for BSR T7/5 cells - (reply: 2)
     
421. No proteins after HeLa cell lysis - (reply: 3)
     
422. How can i fix cultured cells on glass slides for insitu hybridization - (reply: 1)
     
423. Blocking a protein in cell culture through antibody - (reply: 10)
     
424. How to propagate Electro-competent DB3.1 cells - (reply: 6)
     
425. Number of cell - (reply: 5)
     
426. Difference between PFA, acetone and methanol in cell fixation - (reply: 1)
     
427. cell proliferation statistics methods - (reply: 4)
     
428. Losing cells during fixation - (reply: 2)
     
429. ovarian cancer cell line: phenotype disappears - (reply: 3)
     
430. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
     
431. HeLa Cells on Chin/in Nose? - (reply: 7)
     
432. Can centrioles be seen when the cell is not undergoing cell division? - (reply: 3)
     
433. How much ethanol (in micro g) has toxic effects on cells? - (reply: 1)
     
434. penicillin/streptomycin in cell culture - (reply: 3)
     
435. Recombinant expression of cytokines in mammalian cells without the propeptide re - (reply: 1)
     
436. Cell lysis for unknown reasons - (reply: 1)
     
437. Preparing cells for plating - (reply: 3)
     
438. ChIP with low cell number - (reply: 2)
     
439. Mammalian cell culture and G418 selection - (reply: 1)
     
440. Losing cells during intracellular staining method! - (reply: 1)
     
441. G418 mammalian cell selection question - (reply: 3)
     
442. DIFFERENCES BETWEEN GLIAL AND GLIOMA CELL? - (reply: 4)
     
443. Bought HEK293T cells from ATCC -- passage number question - (reply: 1)
     
444. Cell lines selected with G418 but no overexpression of GOI - (reply: 3)
     
445. cell pellet question - (reply: 2)
     
446. B cells cultures. Immunoglobuline production - (reply: 1)
     
447. ChIP problem - using cells as a negative control - (reply: 7)
     
448. Blood cell migration from mother to fetus - (reply: 1)
     
449. Qiagen mini prep cell fragment pellet - (reply: 2)
     
450. Cell death detection using adenosin kinase - (reply: 5)
     
451. THP-1 differentiation using PMA yielding black dots within cell structure - affe - (reply: 4)
     
452. Transfecting three plasmids into hES cells - (reply: 2)
     
453. Agglomeration of suspension cells (T-cells) - (reply: 1)
     
454. Selection of E.coli competent cells - (reply: 2)
     
455. Chelating Aluminum from cell culture - (reply: 5)
     
456. chemotherapeutic-resistant cell lines - (reply: 1)
     
457. infection assay-buffer that lyses mammalian cells but not bacteria - (reply: 1)
     
458. HEK293 vs. SH-SY5Y Cell lines? Which best for in vitro Alzheimer's Model? - (reply: 2)
     
459. Selection of needle size to homogenize cells for RNA extraction - (reply: 3)
     
460. Electroporate lentiviral vector for stable cell line - (reply: 2)
     
461. Place to buy (soluble) ligands for cell culture. - (reply: 3)
     
462. NIH 3T3 cells cross contamination - (reply: 1)
     
463. NIH 3T3 cells - (reply: 3)
     
464. Cells adhering to walls of conical tube? - (reply: 7)
     
465. PI cell cycle analysis and apoptosis - or would a different stain be better? - (reply: 2)
     
466. choosing the correct subculturing ratio - ovarian cancer cell lines - (reply: 1)
     
467. RAW 264.7 cell line - (reply: 4)
     
468. washing of attached bacterial cells - (reply: 3)
     
469. quick and non-expensive method of testing cross cell contamination? - (reply: 4)
     
470. Cell Debris Isolation From Blood - (reply: 3)
     
471. How to calculate drug amount to use in cell lines - (reply: 3)
     
472. Growing certain number of bacterial cells in LB - (reply: 1)
     
473. T cells or Lymphocytes? - (reply: 2)
     
474. Western Blot with fixed cells - (reply: 5)
     
475. Less than 10 GFP positive cells - (reply: 2)
     
476. Doubt: Cell plating for different cell culture plate formats - (reply: 3)
     
477. How to culture HK-2 cells - (reply: 6)
     
478. competent cells - (reply: 1)
     
479. Debris in cell culture - (reply: 2)
     
480. What housekeeper to use with HEK cells? - (reply: 3)
     
481. Housekeeper for HEK cells? - (reply: 1)
     
482. Type I Collagen Vs. Poly-L-lysine Vs. Gelatin Coated coverslips (NSC34 cells) - (reply: 1)
     
483. Cell-repellent coating / surfaces - Growing cells on beads - (reply: 2)
     
484. C3HT101/2 Cell Line not attaching to T25 flask- nightmare!!!! - (reply: 5)
     
485. Phorbol myristate acetate treatment of J774 cell line - (reply: 1)
     
486. Gene expression profiling with small cell number - (reply: 1)
     
487. Gene expression assay with small cell number - (reply: 3)
     
488. Spontaneous immortalization of mouse cells in vitro - (reply: 1)
     
489. Stem Cell Therapy comes of age? Discussion Topic - (reply: 1)
     
490. cell free DNA and maintaining stability - (reply: 3)
     
491. Freezing Cells For Use In Assays - (reply: 2)
     
492. INS-1E cells migration assay - (reply: 1)
     
493. Short-term storage of HEK293 cells before FACS - (reply: 3)
     
494. Sample storage for ELISA (cell culture supernatant) - (reply: 2)
     
495. Any tips on how to avoid lifting cells in 96well plates - (reply: 3)
     
496. Primary cell line - (reply: 5)
     
497. 2D Image Primary Cell line - (reply: 13)
     
498. will mRNA from different cell line lead to loss of expected product in RT PCR? - (reply: 2)
     
499. Geneticin Resistant Cell Lines and Stable Transfection - (reply: 1)
     
500. To small amount of cells used for shRNA transfection - (reply: 1)
     
501. using cell line to generate standard curve - (reply: 6)
     
502. Autofluorescence in thick structure cluster of cells - (reply: 4)
     
503. Primary culture of fibroblast cells from rat/mouse - (reply: 4)
     
504. Serial dilution of cells - (reply: 3)
     
505. Cloning repetitive sequences in mammalian cells - (reply: 3)
     
506. Do cell signaling antibodies work with 1h RT incubation? - (reply: 2)
     
507. Plant Cells - what are they? - (reply: 1)
     
508. HS-5 and HS27A cells - (reply: 1)
     
509. MC-3T3 cells are not mineralizing... - (reply: 3)
     
510. HS-5 cell culture "question" - (reply: 1)
     
511. Problem with Kasumi-6 cell line - (reply: 1)
     
512. G418(Geneticin) is not killing my cells - (reply: 2)
     
513. Community feedback needed to clarify terms for a Cell Culture Ontology - (reply: 1)
     
514. Quantify total DNA in small cell lysate...? - (reply: 1)
     
515. Trouble validating in multiple cell types - (reply: 1)
     
516. Gating of dendritic cells - (reply: 7)
     
517. Please help me take a look at my cells to see whether is it contaminated - (reply: 3)
     
518. cell binding assay with nanoparticles? - (reply: 2)
     
519. Cell lines for clonal selection - (reply: 3)
     
520. Pelleting cells by gentle centrifugation for addition to media - (reply: 13)
     
521. SH-SY5Y Immortalized cell lines; researching neurotoxicity? - (reply: 3)
     
522. Pelleting bacterial cells - (reply: 1)
     
523. How viable is cell culture for a model? - (reply: 2)
     
524. Help with adherent cell culture - (reply: 7)
     
525. study of receptors in cell lines - (reply: 1)
     
526. Cell line nomenclature - suffixes ? - (reply: 3)
     
527. Maintainence of transformed cells - (reply: 5)
     
528. improving viability of pelleted cells - (reply: 2)
     
529. Buying Ins-1e cells - (reply: 1)
     
530. How do/should cell biologists calculate power of analysis? - (reply: 1)
     
531. Mycoplasma on normal hands? - (reply: 1)
     
532. comparing ADCC effect in 3 cell lines. - (reply: 1)
     
533. Help: Best way to disrupt blood cells to generate blood debris and gDNA to be us - (reply: 4)
     
534. Ric3 NAChR 7 expression in HEK cells - (reply: 1)
     
535. Trypan blue - (reply: 6)
     
536. virus and cell surface receptor interaction - (reply: 4)
     
537. Music Video on Cell Division/Mitosis - (reply: 3)
     
538. How to select competent E. coli cells? - (reply: 7)
     
539. Host cell protein sequence search - (reply: 1)
     
540. qPCR from HEK cells transfected with Plasmid - (reply: 4)
     
541. (best cell lysis method for) protein expression analysis by western blot - (reply: 2)
     
542. Preparing supernatant from cell culture for western blot - (reply: 4)
     
543. Fast/easy way to measure cell size - (reply: 4)
     
544. Which is the best way to generate growth curves for cancer cell lines - (reply: 7)
     
545. Cell number issue - Nile Red staining - (reply: 3)
     
546. Music Video on Cell Division/Mitosis - (reply: 1)
     
547. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
     
548. Integrin B1 Staining in HT1080 cells - (reply: 1)
     
549. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
     
550. My cells won't pellet - any ideas? - (reply: 6)
     
551. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
     
552. EMSA: cell extract vs purified protein - (reply: 2)
     
553. Insect cells transfection reagent - (reply: 1)
     
554. Low Protein Content In Cell Lysate - (reply: 1)
     
555. Can you see GFP after lysis mammalian cells? - (reply: 5)
     
556. Centrifugation speeds for cells. - (reply: 8)
     
557. Cell recovery from ethanol fixation - (reply: 4)
     
558. Cryopreservation of MSC at lower cell density - (reply: 2)
     
559. C-terminal GST fusion problem in mammalian cell - (reply: 6)
     
560. Identify cell culture contamination - (reply: 2)
     
561. Adipose stem cell culture - (reply: 5)
     
562. Determine cell proliferation by viability assay ? - (reply: 6)
     
563. Apoptosis via cell to cell contact - (reply: 1)
     
564. Cells stop to grow - (reply: 6)
     
565. Pore formation in cells after adding amphotericin B in culture medium - (reply: 2)
     
566. Long Treatment, Rapidly Dividing Cells - (reply: 7)
     
567. Need help calculating cell number to plate - (reply: 1)
     
568. Injection of human cell culture conditioned media into nude mice - (reply: 1)
     
569. How long can I store cell lysis buffer at 4C for? - (reply: 1)
     
570. Heeeeeelp..cell cycle sample..is it possible to store it? - (reply: 2)
     
571. Subculture issue of primary cell - (reply: 6)
     
572. Differentiating mouse from human cells with PCR - (reply: 5)
     
573. primary valve interstitial cells transfection - (reply: 1)
     
574. What to do about mycoplasma? - (reply: 9)
     
575. Stem Cell Donation - (reply: 7)
     
576. isolating DNA from cells in suspension - (reply: 5)
     
577. Software for designing cell pathways - (reply: 1)
     
578. Tamoxifen citrate solubility for cell work?? - (reply: 1)
     
579. Centrifuged cells stick to wall of reaction tube - (reply: 12)
     
580. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
     
581. Mycoplasma Test Kit - (reply: 1)
     
582. Changing Genomic Regions in Host Cell - (reply: 1)
     
583. TRYPSIN - (reply: 4)
     
584. How to transform adherent cells to suspension (CHO cells) - (reply: 2)
     
585. my competent cell grow on any agar plate - (reply: 7)
     
586. Bacterial Contamination in HuEmbryonic cell lines seen in SFM4 but not in DMEM + - (reply: 3)
     
587. Cell attachement occuring after 48 hours, not 24 hours. - (reply: 3)
     
588. Transfection of a non human gene into human cell line - (reply: 1)
     
589. Procedures to replace vial of cells and passage number. - (reply: 4)
     
590. Immunofluorescence on cells - can I keep the coverslips unstained in PFA? - (reply: 4)
     
591. p14ARF and MDM2 in transformed cells - (reply: 1)
     
592. RNA isolation with low number of cells (1*10^4 to 5*10^4) for RT-PCR - (reply: 3)
     
593. contamination of human cell lines with yeast - (reply: 3)
     
594. Generation of single cell suspensions from neural tissue - (reply: 2)
     
595. Is this contamination or cell death? - (reply: 7)
     
596. BaF3 cell culture in 96 well plates - (reply: 2)
     
597. THP-1 differentiated cells detach when washing with PBS (Mg and Ca free PBS) - (reply: 2)
     
598. unwanted cell lysis during LB+Amp incubation - (reply: 5)
     
599. Cells spontaneously detaching? - (reply: 1)
     
600. Immuno-staining for cell surface/membrane markers after permeabilisation - (reply: 4)
     
601. different type of media for cell culture - (reply: 1)
     
602. Influenza: cell and mechanism information? - (reply: 1)
     
603. Can CMV promoter start transcription in prokaryote cells - (reply: 1)
     
604. How to treat cells with TNFa - (reply: 2)
     
605. CLOUDINESS of RIN5F cell culture - (reply: 7)
     
606. U87MG cells not growing well - (reply: 3)
     
607. Concentrating low volume of cells - (reply: 8)
     
608. Preparing a conditioned medium from cancer cells - (reply: 3)
     
609. Presence of melanin in melanin containing cell line - (reply: 1)
     
610. Cancer cells culture - (reply: 1)
     
611. How to achieve uniform cell distribution in culture vessle - (reply: 6)
     
612. viability/integrity stain of fungal cells - (reply: 5)
     
613. Cell culture contamination - (reply: 3)
     
614. airway primary cells stop growing at passage 3 - (reply: 1)
     
615. cell culture neural cells easier? - (reply: 6)
     
616. Question - Corticosterone, cell culture - (reply: 1)
     
617. pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 30)
     
618. Where to obtain rat embryonic stem cells? - (reply: 3)
     
619. Stable cell line - (reply: 1)
     
620. PBMC cells handling - (reply: 1)
     
621. myc-tag antibody makes multiple bands, even in mock & parental cells - (reply: 1)
     
622. Unable to clone in E. coli cells - (reply: 3)
     
623. removing MHC class I from the cell surface - (reply: 3)
     
624. Osteosarcoma cell culture problem - (reply: 7)
     
625. rtPCR stem cell signalling array from quiagen- has anybody experience? - (reply: 1)
     
626. Thawed cells spent 4 hours extra in DMSO.. problem? - (reply: 4)
     
627. Thawed cells spent 4 hours extra in DMSO.. problem? - (reply: 1)
     
628. colon cancer cell lines vs normal colon epithelial cells - (reply: 1)
     
629. Cell count and seed, plz help... - (reply: 4)
     
630. In and out of cell Help 2 - (reply: 6)
     
631. In and out of cell Help - (reply: 2)
     
632. Primary culture of breast tumor cells - (reply: 1)
     
633. Strange Cell Morphology & Cell Debris Following Lentiviral Transduction - (reply: 2)
     
634. Cell numbers versus confluency - (reply: 2)
     
635. Flow cytometry in THP-1 cells - (reply: 1)
     
636. Competent bacterial cells - (reply: 2)
     
637. Could a change of FBS affect outcome of a cell proliferation assay? - (reply: 4)
     
638. How do they make passage limited cells? - (reply: 1)
     
639. transient transfection problem using ME180 cells - (reply: 1)
     
640. How to completely remove cells from surface - (reply: 3)
     
641. Hiperfect siRNA in HT-29 cells help - (reply: 2)
     
642. Isolation of BOTH cells AND serums from mouse blood - (reply: 5)
     
643. Transfection of pCI-neo plasmid into 3T3-L1 cells - (reply: 1)
     
644. Commercial source Stomach Smooth Muscle Cell lysate - (reply: 1)
     
645. DMEM or EMEM Does it matter? (HeLa Cells & Transfection) - (reply: 3)
     
646. cell line contaminated by something... - (reply: 2)
     
647. Flag Staining in U87 cells - (reply: 4)
     
648. protein is not expessing in HEK 293 cells - (reply: 2)
     
649. competent cells: CaCl2 autoclave vs filter sterilisation - (reply: 12)
     
650. HepG3 cell line origin - (reply: 3)
     
651. dilution of cell suspension - (reply: 4)
     
652. how to preserve spleen cells for long duration, for flowcytometry - (reply: 2)
     
653. Increasing cell growth rate in plain M9 medium - (reply: 1)
     
654. Puromycin - shRNA - stable cell line for knock down - (reply: 4)
     
655. Reducing cell adhesion - (reply: 1)
     
656. The Hek 293T cell line looks like non adherent cells after to be sorted in FACS - (reply: 1)
     
657. Hygromycin selection of virally-transduced cell lines - (reply: 1)
     
658. Which cells express which genes? - (reply: 1)
     
659. IGF1 stimulation of THP1 cells - (reply: 2)
     
660. Cells detach during bodipy 493/503 staining - (reply: 1)
     
661. RNA isolation from small numbers of cells - (reply: 6)
     
662. Distinguish murine and human cells by surface staining? - (reply: 1)
     
663. starting HaCaT cell line. basic questions - (reply: 6)
     
664. culture media for pancreatic cancer stem cells - (reply: 2)
     
665. Lysis method for single human embryonic cell - (reply: 5)
     
666. In vitro transcription/translated protein to cells!!! - (reply: 1)
     
667. Cell plating - (reply: 2)
     
668. sample preparation from whole cell lysates for Edman degradation - (reply: 2)
     
669. colony formation assay in A2780 cells - (reply: 4)
     
670. Cell confluency, transfection, protein expression for 293T cells - (reply: 2)
     
671. Which is a better way to culture cells after thawing ? - (reply: 2)
     
672. Comet assay sperm cells - (reply: 5)
     
673. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
     
674. Live/Dead Staining of already Permeable Cells - (reply: 10)
     
675. cell synchronisation for fibroblasts (help choosing method/chemical) - (reply: 2)
     
676. Cell Therapies - (reply: 2)
     
677. What is the activity of 1X Trypsin solutions? - (reply: 3)
     
678. SURE E. coli competent cells - (reply: 1)
     
679. Stable protein expression in Vero cells - (reply: 2)
     
680. HL60 cell line suppliers - (reply: 1)
     
681. Insoluble material floats over my SDS lysate of human cells after centrifugation - (reply: 7)
     
682. Cell Fixation for DNA flow cytometry in fibroblasts - (reply: 2)
     
683. Question about HeLa cell overconfluence - (reply: 3)
     
684. 293T cell contamination post-transfection - (reply: 1)
     
685. HEK293T cells are too fragile?? - (reply: 2)
     
686. number of splitting after defrozing cells - (reply: 2)
     
687. my human iPS cells have a lot of differentiation - (reply: 4)
     
688. Properly designing a drug treatment assay measuring cell cycle arrest - (reply: 2)
     
689. Detaching Breast Cancer cells (MCF-10A, HCC70) without trypsin- used for FACs - (reply: 4)
     
690. counting cells before adding them to 96 well plate - (reply: 7)
     
691. Cell Suspension - (reply: 2)
     
692. What will happen if the cell culture medium is suddenly changed to another one? - (reply: 5)
     
693. MDA-MB-231 cells undergoing senescence- help! - (reply: 2)
     
694. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
     
695. coating cover-slides before seeding cells for fluorescent microscopy - (reply: 4)
     
696. How to induce early apoptosis in cells without using chemicals or UV - (reply: 5)
     
697. choice of cell line for orthotopic xenograft implant - (reply: 3)
     
698. Compound concentration depending on cell number? - (reply: 4)
     
699. How to remove mycoplasma contamination in cell lines? - (reply: 5)
     
700. [Question] How to remove cell debris stuck on the attached cells in primary card - (reply: 2)
     
701. gene overexpression in cells: virus transduction or transfection following selec - (reply: 4)
     
702. Reagent used to adjust pH of cell culture media - (reply: 3)
     
703. Powder indomethacin in cell culture media?? - (reply: 2)
     
704. How many generation of HEK293 cell can undergo? - (reply: 1)
     
705. [Question] how to make primary-cultured cells evenly distributed? - (reply: 11)
     
706. small dots in cell culture - (reply: 2)
     
707. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
     
708. Clumpy competent cells - (reply: 3)
     
709. Preparation of bacterial cells for SEM and TEM - (reply: 2)
     
710. G418 advice in estab;lishing stable cell line - (reply: 1)
     
711. Electroporation of J774.2 murine macrophage cell line - (reply: 1)
     
712. About trypsinizing adherent cells in 96-well plates - (reply: 2)
     
713. Passaging cells - (reply: 12)
     
714. Preparing homemade XL1-blue cells in CCMB80 buffer - (reply: 1)
     
715. Understanding Cell Passaging - (reply: 5)
     
716. How to extract DNA from small number of cells - (reply: 2)
     
717. How to identify beta pancreatic cells in dish of heterogenous cells? - (reply: 12)
     
718. Cell Splitting Ratio Question - (reply: 5)
     
719. Sf9 cell suspension culture - (reply: 5)
     
720. competent cell formation - (reply: 1)
     
721. Help! Clontech Xfect is killing my T-Rex HeLa cells!!! Why! - (reply: 1)
     
722. How long are cells viable on ice - (reply: 2)
     
723. Discrepancy between tissue and cell culture - (reply: 1)
     
724. What is the density of a human skin cell? - (reply: 8)
     
725. Determining the % of GFP/CHERRY cells in co-mixed culture - (reply: 3)
     
726. Is the genomic DNA similar among different cell lines? - (reply: 4)
     
727. serumm free culture of adherent cho cells - (reply: 1)
     
728. detection of mesenchymal stem cells in cancer tissue sample - (reply: 1)
     
729. Can u suggest any myoepithelial cell isolation protocol with the help of reagent - (reply: 1)
     
730. cell line cultured in suspension - (reply: 1)
     
731. GFP cells in paraffin.....orange? - (reply: 1)
     
732. overexpression using pcDNA3.0 in stably knockdown cells - (reply: 3)
     
733. Cell staining for fluorometric assay - (reply: 5)
     
734. Would E. Coli cells be stable at room temp, if in media with glycerol? - (reply: 1)
     
735. Long-Term Culturing of SH-SY5Y Cells - (reply: 1)
     
736. N acetyl cysteine solution for cell culture - (reply: 3)
     
737. Aortic smooth muscle cell contamination - (reply: 1)
     
738. need recommendation for stable transduction of fibroblast cells - (reply: 1)
     
739. Sub G1 in cell cycle - (reply: 2)
     
740. DMSO induces cluster growing in LNCaP cells? - (reply: 1)
     
741. Cell cycle arrest - adherent cells going into suspension - (reply: 4)
     
742. Prevent adherent cells from adhering - (reply: 3)
     
743. INS-1E cells not adhere to the flask! - (reply: 1)
     
744. A549 cells are not attaching to flask - (reply: 2)
     
745. irradiated J774 cells - (reply: 1)
     
746. Cell lysis with proteinase k - (reply: 2)
     
747. One siRNA sequence, many cell lines - (reply: 1)
     
748. Freezing cell lysates for westerns - (reply: 2)
     
749. SDS-PAGE for cell culture medium - sample preparation - (reply: 1)
     
750. adherence to host cell question - (reply: 2)
     
751. New patch of sf9 cells not growing - (reply: 2)
     
752. contamination in yeast cell culture - (reply: 8)
     
753. Tissue culture contamination like bacteria inside the cells. What is this kind o - (reply: 1)
     
754. sf9 Insect cells questions - (reply: 3)
     
755. Kit to mesure cell death - (reply: 4)
     
756. An improve method for making Competent Cells without heat shock. How? - (reply: 2)
     
757. J774 and Flow cytometry....are they really big cells ? - (reply: 9)
     
758. immunostaining cells grown in matrigel - (reply: 1)
     
759. Cell concentration for flow cytometry - (reply: 2)
     
760. Transient Transfection: Adherent vs. Suspended/Floating Cells - (reply: 7)
     
761. Cells growing very slowly - (reply: 6)
     
762. SH-SY5Y cells do not grow - (reply: 1)
     
763. GH3 cells - (reply: 4)
     
764. The connection between LPS and PMA stimulation for maturation of ThP1 cells - (reply: 2)
     
765. Using normal competent cells in electroporation method? - (reply: 1)
     
766. Washing n2a cells out of 75 cm flask? - (reply: 1)
     
767. How to dissociate N2A cells after centrifugation? - (reply: 3)
     
768. Undefinable particles in cell culture flask - (reply: 2)
     
769. Help: transduction of primary blood cells (T): lentiviral vs. adenoviral vector - (reply: 1)
     
770. immunostaining of cells in culture - (reply: 1)
     
771. healthy stem cells vs cancer stem cells - (reply: 1)
     
772. values for mRNA molecules per cell - (reply: 1)
     
773. Help!! need to culture PC3M cell line. Any advice? - (reply: 5)
     
774. MCF10A Tet-ON stable cell line generation - (reply: 1)
     
775. Primary cell line - (reply: 2)
     
776. Neurosphere Assay with neuroblastoma cell lines - (reply: 1)
     
777. How to decontaminate adherent cells with gentamicin - (reply: 2)
     
778. Contamination in CHO cell ? - (reply: 3)
     
779. Thawing Hek 233t cells- but cells dying!! - (reply: 3)
     
780. How to purify RNA from a handful number of cells? - (reply: 5)
     
781. Check cell viablity after FACS - (reply: 2)
     
782. ISH on fluorescing cells - (reply: 1)
     
783. Please help, may i replenish Glutamine in cell culture medium? - (reply: 3)
     
784. Protein extraction from dead cells - (reply: 1)
     
785. Cells in suspension vs. plated - (reply: 2)
     
786. PI stain for cell viability~~Compensation? Suggestion needed~~^^ - (reply: 5)
     
787. Hepg2 cells growing on top of each other - (reply: 1)
     
788. urgent! calculating concentration of compound with different cell amounts - (reply: 3)
     
789. h9c2 cells culture - (reply: 5)
     
790. Does anyone have experience of subjecting cultured cells to mechanical stress? - (reply: 1)
     
791. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
     
792. cell population doubling - (reply: 1)
     
793. Seeding HeLa cells into 96 well plate for transfection - (reply: 2)
     
794. Cell line licence - (reply: 1)
     
795. adenoviral cre or lentiviral cre for mouse primary cells? - (reply: 2)
     
796. temperature tolerance of animal cells - (reply: 3)
     
797. only one plasmid expressed highly after co-tranfection into cells - (reply: 1)
     
798. pERK and pAKT in MDA-MB-231 cells by western blot! - (reply: 1)
     
799. Human insulin for cell culture? - (reply: 2)
     
800. violet laser (405nm) and cell cycle staining with DAPI - (reply: 1)
     
801. Competent cells question: JM101 vs DH5alpha - (reply: 2)
     
802. Trouble producing stable cell lines - (reply: 2)
     
803. Infecting cells with virus - (reply: 1)
     
804. Requesting basic protocols on cell sorting - (reply: 1)
     
805. lysing mammalian cells in sds - (reply: 3)
     
806. Is it possible to extract RNA from Cells frozen in 10% DMSO ? - (reply: 2)
     
807. Is it possible to assay the carbohydrate profile of a cell? - (reply: 2)
     
808. changing cell culture media - (reply: 1)
     
809. Preparing Palmitate:BSA solution for treatment of cells? - (reply: 7)
     
810. trypsinization of adherent cells - (reply: 9)
     
811. Mitochondria pelleted together with nuclei from 293 Cells using Sucrose gradient - (reply: 3)
     
812. Aberrant (apparently) nuclear immunostaining for VEGF in Glial cells - (reply: 2)
     
813. How to lysis cells for an RNA extraction - (reply: 1)
     
814. Problem with THP-1 cell line - (reply: 3)
     
815. normalization to protein content or cell number - (reply: 2)
     
816. detection of phosphorylation from cell pellet - (reply: 2)
     
817. are THP-1 Cells(Monocytes) or Macrophages resistant to Mycoplasma infection or - (reply: 4)
     
818. Recommended competent cell for transformation of large plasmids? - (reply: 4)
     
819. Cell culture shared with yeast? - (reply: 2)
     
820. E. coli cells refuse to grow! - (reply: 2)
     
821. how to fraction stem cell at different cell cycle stage - (reply: 2)
     
822. Making Nuclei From Mouse Cells - (reply: 1)
     
823. Different cell lines different protein stability - (reply: 1)
     
824. Neutrophil cell lines and plasma cell lines of choice - (reply: 1)
     
825. Immunofluorescence Staining of Cells - (reply: 1)
     
826. Nucleus fills the whole cell - (reply: 3)
     
827. Generating stable cell line with linear DNA - (reply: 3)
     
828. isolating cells from Cockroach - (reply: 2)
     
829. Question about suspension cells - (reply: 7)
     
830. Calu-3 cell line do not grow properly - (reply: 1)
     
831. Detach cells and stop stimulation simultaneously- How? - (reply: 4)
     
832. Counting and plating cells - (reply: 3)
     
833. cells die 4-6 days after thawing - (reply: 8)
     
834. what is the trypsin X for subculturing - (reply: 1)
     
835. Cell Envelope Proteins Smeared - (reply: 1)
     
836. Calculating the amount of the desired cells in the medium ? - (reply: 3)
     
837. long term storage of cells at -80 - (reply: 1)
     
838. Trypan Blue Exclusion Test - (reply: 2)
     
839. Making BL21 competent cells - (reply: 2)
     
840. cell plating density - (reply: 2)
     
841. What kind of cell culture contamination is it? - (reply: 8)
     
842. Problem with preparing unicellular suspension from MCF7 cells - (reply: 3)
     
843. xenograft cells harvested from spleen of SCID/NOD mice - (reply: 1)
     
844. to treat cells with inhibitor - (reply: 2)
     
845. lysis buffer not lysing adherent cells? - (reply: 8)
     
846. Trypan Blue Staining - (reply: 3)
     
847. Culturing cells on a slide - (reply: 5)
     
848. Culturing cells on a slide - (reply: 3)
     
849. protocol for immunostaining primery cells - (reply: 5)
     
850. How to wipe out fragment of dead cells from the suspension cells? - (reply: 1)
     
851. How to Dilute Cell Lysate for Luciferase Assay - (reply: 2)
     
852. What's the function of cholera toxin in cell culture medium? eg. MCF10A cell - (reply: 2)
     
853. Could the use of different FBS affact my cells - (reply: 5)
     
854. Analysis of high throughput screen for cell surface markers - (reply: 1)
     
855. Lymphoblastoid cell culture - (reply: 9)
     
856. SF9 Cells lose exponential growth, lack stationary phase - (reply: 3)
     
857. Don't know why my cells are unhealthy - (reply: 2)
     
858. Hybridoma cells not secreting antibody - (reply: 3)
     
859. Determining gene of interest from Cell culture lines - (reply: 2)
     
860. Three clones of a cell line? - (reply: 3)
     
861. Freezing cells in DMSO straight into minus 80? - (reply: 4)
     
862. MCF7 cells vitality - (reply: 3)
     
863. Ultrasonic bath for cell lysis - (reply: 3)
     
864. any methods to evaluate the attaching ability of suspension cells? - (reply: 6)
     
865. Vascular Smooth Muscle cell Marker - (reply: 3)
     
866. Foam in Cell Culture - (reply: 1)
     
867. Where can I find more info on how certain ionic solutions can affect cell biolog - (reply: 4)
     
868. SURE cells - (reply: 1)
     
869. DT40 Cell Synchronisation - (reply: 2)
     
870. Bio-Rad Cell counter - (reply: 3)
     
871. SKBR-3 cells dying - (reply: 3)
     
872. New cell culture user with no teacher or trainer - (reply: 5)
     
873. Help with some problems with Culturing Epithelial Cells - (reply: 5)
     
874. NIH3T3 cells are suddenly dying a few days after thawing and expanding - (reply: 1)
     
875. Stable cells survive with antibiotics but no exogenous expression detected with - (reply: 2)
     
876. What are these large multinucleated cells??? - (reply: 1)
     
877. Searching for a live cell staining method - (reply: 1)
     
878. WT BRAF/NRAS mouse Melanoma cells - (reply: 2)
     
879. HEK 293 cells Detachement - (reply: 8)
     
880. Cell culture in candle jar - (reply: 2)
     
881. centrifugation speed/cell prep for flow - (reply: 4)
     
882. Huge background with HT22 cells phophoERK antibody - (reply: 3)
     
883. Huge background with HT22 cells phophoERK antibody - (reply: 1)
     
884. Competency cells analysis - (reply: 1)
     
885. How many cells needed for co-IP and mass spec - (reply: 4)
     
886. Performing MTT assay in suspension cells (new in the subject) - (reply: 8)
     
887. cells die after transfection - (reply: 1)
     
888. Seeding cells on small metal screws - (reply: 1)
     
889. Cell lysate turbidity upon long storage - (reply: 3)
     
890. Host cells for transformation - (reply: 3)
     
891. Splitting or feeding cells for the weekend - (reply: 1)
     
892. Black moving particles in the cell Culture - (reply: 2)
     
893. lymphocyte proliferation using trypan blue - (reply: 2)
     
894. cell culture - (reply: 1)
     
895. cells shrink, detach from each other when changing media - (reply: 3)
     
896. Newbie help: Seeding cells to 6-well plates - (reply: 2)
     
897. 3t3 cell tured black - (reply: 9)
     
898. calculating the number of cells to freeze down - (reply: 2)
     
899. Fixed cells, sticky cells? - (reply: 2)
     
900. What is cotransfection in molecular cell biology? - (reply: 1)
     
901. BSA in adipocyte cellculture? Alternatives?? - (reply: 1)
     
902. serum deprivation of cell lines - (reply: 1)
     
903. serum deprivation of cell lines - (reply: 1)
     
904. What to do Post-transfection of cells with siRNA? - (reply: 2)
     
905. Problem with HEK stable cell line - (reply: 1)
     
906. HEK 293 cell culture - (reply: 3)
     
907. HepG2 cells resistant to Trypsinization - Please help!! - (reply: 8)
     
908. difficulty in passaging HK-2 cells - (reply: 5)
     
909. difficulty in passaging HK-2 cells - (reply: 2)
     
910. qPCR in primary cells - housekeeping CT-values sample-specific bad - (reply: 1)
     
911. Expression of protein X varies in different w.b running (in one cell line, but - (reply: 3)
     
912. More cells in an antibiotic plate than in a plate without antibiotic???? - (reply: 2)
     
913. what are these cells?? - (reply: 1)
     
914. How to choose the right plasmids for certain types of cell? - (reply: 1)
     
915. Isolation of rat endothelial cells - (reply: 2)
     
916. protocol for making e.coli competent cells under low temperature - (reply: 1)
     
917. Looking for cell surface markers. - (reply: 3)
     
918. common nuclear marker for T cells - (reply: 1)
     
919. How is the cell protected from the hydrolase enzymes of the lysosomes? - (reply: 1)
     
920. Any reliable markers for mouse memoery T cells? - (reply: 1)
     
921. Do mouse endothelial cells express the alphaVbeta3 integrin? - (reply: 1)
     
922. Cell Culture debris or contamination? - (reply: 4)
     
923. primary cell isolation from CNS, how to remove myeline debris - (reply: 1)
     
924. Platinum-A cell trasnfection toruble - (reply: 1)
     
925. cell culture media refrigeration - (reply: 4)
     
926. abnormal epithelial cells in mouse mammary gland - (reply: 1)
     
927. cell DNA content of cells grown on different scaffolds - (reply: 2)
     
928. Cancer cell starvation - (reply: 1)
     
929. Why are HEK-293 cells difficult to perform clonal selction? - (reply: 4)
     
930. Housekeeping gene for COS-1 cells - (reply: 1)
     
931. applying cell culture protocol of protein extraction for rat intact tissue is co - (reply: 1)
     
932. Monocytes die after cell scraping - (reply: 2)
     
933. The water pan got dry in the TC incubator. Will my cells be OK? - (reply: 1)
     
934. Cell fractionation - (reply: 2)
     
935. Media formulations for MDA-MB-231, BT-474 cells and Caco-2 cells - (reply: 1)
     
936. Proper storage of isolated white blood cell prior to RNA isolation - (reply: 3)
     
937. Dropped cells into liquid nitrogen tank - (reply: 3)
     
938. 5-FOA media not letting ANY cells grow... help! - (reply: 1)
     
939. Small intestinal cell line for transport studies - (reply: 4)
     
940. help needed in determining volume of cells to be seeded - (reply: 6)
     
941. Dilution, Cell concentration & DNA content - (reply: 3)
     
942. competent cells - (reply: 2)
     
943. Cell lines & gene of interest - (reply: 1)
     
944. Software for selection genes related to concrete cell processes - (reply: 1)
     
945. Immunocytochemistry with HEK293 cells - (reply: 1)
     
946. How to determine apoptotic cell types in-vivo? - (reply: 1)
     
947. Isolating Genomic DNA from a small colony of cultured human cells - (reply: 1)
     
948. data analysis for single cell size and distribution - (reply: 1)
     
949. determining optimal cell count for MTT - (reply: 2)
     
950. Extracting DNA from FACS sorted cells - (reply: 3)
     
951. Storing frozen cells in -80C instead of liquid nitrogen - (reply: 7)
     
952. phosphate + freeze thaw vs RIPA buffer for cell lysis - (reply: 2)
     
953. DG44 CHO cells and Methotrexate - (reply: 1)
     
954. extract plasmids from transfected eukaryotic cells - (reply: 6)
     
955. Trypsin Deactivation - (reply: 4)
     
956. Staining Cells in Suspension - (reply: 4)
     
957. cell culture confusion - (reply: 1)
     
958. a gene expressed in 293 cell lines but not Hela cells - (reply: 1)
     
959. chambered cell culture-slide for confocal microscopy - (reply: 3)
     
960. Nicotiana tabacum (BY-2) Suspension cells contaminated! Help! - (reply: 1)
     
961. urgh...mycoplasma drama..... - (reply: 5)
     
962. Dead or Alive... -_- (MCF-7 cells) - (reply: 7)
     
963. how to wash cells after fixation for a live binding study - (reply: 1)
     
964. Evenly distribute 100ul of cells in a 48/96 well - (reply: 2)
     
965. Cell lysis before shearing - (reply: 5)
     
966. really low puromycin concentration for selection of cells - (reply: 6)
     
967. Single stain control for 293 cell line - (reply: 4)
     
968. Is mCherry denatured by Trypsin or RIPA buffer? - (reply: 7)
     
969. loading dendritic cell with antigen - (reply: 1)
     
970. Cell Lysis and Western Blotting - (reply: 1)
     
971. problem with embryo carcinoma cell line , p19 , - (reply: 1)
     
972. In vitro induction of apoptosis in tumor cells by inactivated NDV and IAV - (reply: 1)
     
973. problem with embryo carcinoma cell line , p19 , - (reply: 1)
     
974. Recombinant protein from cell lysate - (reply: 4)
     
975. How to test if a compound is entering cells? - (reply: 3)
     
976. help with cell seeding - (reply: 2)
     
977. SURE or Stbl competent cells for retroviral vector? - (reply: 2)
     
978. RNA isolation from frozen cells - (reply: 2)
     
979. How do you coat coverslips before seeding cells? - (reply: 3)
     
980. Buy.Ask for buying some cell lines and antibodies - (reply: 3)
     
981. competentce cell, cloning - (reply: 2)
     
982. Flp-recombinase target site integration in 293 T-REx cells - (reply: 1)
     
983. Storage of transfected PC12 cells - (reply: 1)
     
984. cell adhension - (reply: 1)
     
985. Using Methanol For Cell Lysis - (reply: 1)
     
986. Adjusting pH of cell culture medium for ELISA - (reply: 1)
     
987. Help finding siRNA targets in MEF and HEK293 cells needed - (reply: 4)
     
988. Help needed with stable cell line creation - problems making a cell line expressing a viral protein (reply: 3)
     
989. Contamination of primary cells with fibroblast. - (reply: 1)
     
990. Help With Lysing HEK293 & Fibroblast Cells - (reply: 1)
     
991. problem with siRNA transfection of HEK cells - (reply: 5)
     
992. Splitting A549 cells - (reply: 9)
     
993. NMDA Transfection in 293 Cells - (reply: 1)
     
994. freezing adherant cells - (reply: 2)
     
995. freezing adherant cells - (reply: 2)
     
996. Total cell lysate from T cells - hard to lyze all the nuclei with RIPA (reply: 2)
     
997. How the heck does serum activate Akt?!? - Is serum starvation necessary in endothelial cells? (reply: 1)
     
998. cells stably expressing T7 RNA polymerase - (reply: 1)
     
999. Cell Fixation - Experience with cell fixation? (reply: 5)
     
1000. picking up colonies and growing in the broth after transformaion - cloning with competent cells (reply: 1)
      
1001. Is pCL-Eco suitable for packaging viruses that used to infect human cells? - (reply: 1)
      
1002. Cause of Death for Cell Culture? - (reply: 5)
      
1003. creat a SY5Y stable cell line - (reply: 1)
      
1004. selecting drug-resistant colon cancer cells - (reply: 2)
      
1005. Help, problems with expressing human proteins in E.coli - IPTG induced cells turn pink (reply: 1)
      
1006. Helper T cell skewing - (reply: 3)
      
1007. Optimum serum starvation period for NIH, CHO, COS7 cells - (reply: 4)
      
1008. Help me brainstorm an explanation for this cell culture contamination - Cell Culture, contamination, NIH 3t3 (reply: 7)
      
1009. competent cells stock - (reply: 3)
      
1010. intracellular cytokine staining - re-stimulation of activated T cells (reply: 1)
      
1011. DH5alpha cells - (reply: 4)
      
1012. pheonix cells and &293A cells - (reply: 1)
      
1013. could freezing/thawing make mammalian cells competent - could it change phenotype back? (reply: 6)
      
1014. Protein purification from HEK 293 cells - (reply: 1)
      
1015. Growing hybridoma cells in medium without serum - (reply: 2)
      
1016. Cells sensitive after replacing media - what am I doing wrong? - (reply: 4)
      
1017. Cell drawing tools? - (reply: 6)
      
1018. How many cells in plant embryos ? - seed biology (reply: 1)
      
1019. cell count - (reply: 8)
      
1020. question on making chemical and electrocompetent cells - (reply: 2)
      
1021. COS7 cells giving me problems - (reply: 1)
      
1022. Where to get 5T series cells? - (reply: 1)
      
1023. cell number for colony formation - HEK293, NIH3T3 (reply: 1)
      
1024. Electroporation/electrotransfection mammalian cells - (reply: 7)
      
1025. Cell debris here, there, everywhere!? - (reply: 2)
      
1026. Cell debris here, there, everywhere!? - (reply: 1)
      
1027. Mini prep of frozen bacteria cells - (reply: 2)
      
1028. Help! Turned on UV light. Did I kill my cells? - (reply: 2)
      
1029. growing competent cells - (reply: 1)
      
1030. Freezing density for NIH 3T3 cells - (reply: 3)
      
1031. Stable Cell Line generation - (reply: 3)
      
1032. making more competent cells - (reply: 2)
      
1033. CellTracker dye in necrotic cells? - (reply: 1)
      
1034. The case of the punky Sf9 cells - (reply: 2)
      
1035. sticky perm cells (tween 20)- can I prevent it? - (reply: 2)
      
1036. Preparation of mammalian cell lysis - Preparation of mammalian cell lysis for Western blotting (reply: 7)
      
1037. collecting cells HT1080 - (reply: 4)
      
1038. Expired media - How long after the expiration data is cell culture media good? (reply: 2)
      
1039. Help: problem with MDCK cells - (reply: 2)
      
1040. bacterial Cell free culture supernatants - Can any one help me out to prepare a cell free culture supernatant (reply: 3)
      
1041. Oh my cell culture problems never cease!!! - (reply: 4)
      
1042. WEHI-3BD cell culturing - Advice on culturing. (reply: 5)
      
1043. cell homogenization - (reply: 13)
      
1044. cell count - (reply: 3)
      
1045. cell lysis - confocal slides (reply: 4)
      
1046. HeLa cell anatomy question - (reply: 3)
      
1047. Need cell lines wich express separately TLR 2, 4 and 5 - (reply: 1)
      
1048. MSC in culture - stop growing? - Mesenchymal stem cells in culture randomly stop expanding (reply: 2)
      
1049. THP-1 cell line - (reply: 3)
      
1050. CaMKII alpha promoter and cell tranfection - (reply: 1)
      
1051. Adaptation of mammalian cells to serum free mode - (reply: 2)
      
1052. Live cell Imaging medium - (reply: 1)
      
1053. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
      
1054. MDA-MB-231 cell transfection - (reply: 5)
      
1055. Looking for protocol to do biotinlynation on each single protein in cell lysate - (reply: 1)
      
1056. Break Cell Clumps - prepare single cells for injections (reply: 1)
      
1057. Stem Cells Sphere - stem cells (reply: 2)
      
1058. Request for cell lines? - (reply: 1)
      
1059. Is 2% pen strep too much for cell culture? - will my cells die a painful death? (reply: 3)
      
1060. MTT problems with Huh7 cell line, plzzzzzzz help - MTT color problem (reply: 5)
      
1061. Calculating cell doubling time - (reply: 1)
      
1062. SPONTAEOUS DEATH OF CELLS in culture - (reply: 8)
      
1063. Infected cells that are dying after puromycin selection - (reply: 1)
      
1064. contamination issue in cell culture - (reply: 1)
      
1065. Why do cells need to be washed in PBS before biotinylation? - (reply: 3)
      
1066. AAD positive means late apoptosis? - About cells that are simultaneously positive for annexin V and AAD (reply: 2)
      
1067. Purification of recombinant proteins from insect cells - (reply: 2)
      
1068. Cell cycle evaluation in cells with more than one nuclei - (reply: 8)
      
1069. Help! Transfection newbie with a dumb question - Trying to transfect adherent cells (reply: 1)
      
1070. Tissue vs cell line lysate - Improve sample quality? - (reply: 4)
      
1071. Growth curve for PC3 cell line - (reply: 1)
      
1072. Agrobacterium EHA101 competent cells - (reply: 1)
      
1073. THP-1 cell line - (reply: 2)
      
1074. Effective DCFDA Protocol for Non-Adherent THP-1 Cells? - (reply: 1)
      
1075. Stripping Buffers that won't kill my cells? - Recipe for 0.1 M Tris-Acetate 2.0 M NaCl pH 7.7 (reply: 3)
      
1076. best freezing method for electrocompetent cells - (reply: 9)
      
1077. cell seeding calculation - (reply: 8)
      
1078. Improved Stem Cell Proliferation - Would interested in improving proliferation of adult stem cells? (reply: 5)
      
1079. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
      
1080. Yeast contamination in cell culture - (reply: 8)
      
1081. Problems with Scrambled Control - Scrambled controls are inhibiting cell growth more than test siRNA (reply: 2)
      
1082. Remove cells from dish and fix them in slides - (reply: 3)
      
1083. Help- lysing cells with triton x-100 - (reply: 1)
      
1084. E.coli cells lyse in minimal media - (reply: 3)
      
1085. How stable are suspension cells in room temperature? - (reply: 2)
      
1086. Help with HELA and HEK293 cell seeding for transfection - (reply: 4)
      
1087. Transfection in insect cell lines?? - (reply: 1)
      
1088. PCR genomic DNA using cell lysate - (reply: 1)
      
1089. HELA Cells Capable of producing whole, infective HPV particles? - (reply: 3)
      
1090. Distilled water for Electrocompetent Cell Preparation - (reply: 6)
      
1091. Should I fix cells digested from tissues? - (reply: 4)
      
1092. STR cell line identification - looking for cell line identification method (reply: 1)
      
1093. Establishing Sub-cutaneous Xenograft Cancer Model - How many millions of cells to inject for subcutaneous models (reply: 3)
      
1094. Rat Dental Pulp Stem Cell Culture - (reply: 1)
      
1095. NK cell analysis using CD16PE and CD56PE - (reply: 2)
      
1096. My neuroblastoma are floating!! please help - Both SK-NSH and SH-SY5Y are adherent cells, but are only floating now (reply: 3)
      
1097. cell suspencion and medium - (reply: 5)
      
1098. One Shot TOP10 Cells - (reply: 6)
      
1099. trypan blue for chlorella - (reply: 1)
      
1100. Help on BmN4 cell culture - How to maintain BmN4 cells? (reply: 1)
      
1101. Compare ChIP to cell lysate - (reply: 1)
      
1102. Compare ChIP to cell lysate - (reply: 1)
      
1103. Testing cytoxixity on healthy human cells - (reply: 2)
      
1104. what cell line to use - to demonstrate ubiquitination (reply: 1)
      
1105. GFP cells intracellular staining - (reply: 1)
      
1106. Xenograph - how to maintain cell viability? - (reply: 1)
      
1107. Western blot of apoptotic cells - (reply: 2)
      
1108. cell sycle and proliferation - (reply: 3)
      
1109. help with cell dilution - (reply: 2)
      
1110. Difficult Cell Proliferation Experiment - (reply: 2)
      
1111. Cell dilution cloning - Cells cloning (reply: 2)
      
1112. Which cells to use ? - Chang liver or normal breast cells ? (reply: 1)
      
1113. HTC116 cells detaching !!!! - (reply: 2)
      
1114. Revival of kasumi-1 cells - (reply: 3)
      
1115. HEK 293 Trypsin vs. only EDTA - (reply: 2)
      
1116. Growth conditions for E. coli DB3.1 cells - (reply: 1)
      
1117. Mycoplasma cleanup time... - (reply: 10)
      
1118. weird & curious problem with CHO cell culture - (reply: 3)
      
1119. HaCat do not grow after using cell scraper - (reply: 5)
      
1120. MTT assay problems (Cells just come off after incubation) - Cells just come off after incubation in 37 degree (reply: 1)
      
1121. Cell Lysates from a Western Blog - (reply: 2)
      
1122. Loss of YFP signal in transduced T cells - (reply: 7)
      
1123. Overexpression of mouse constructs in human cell lines - some questions (reply: 6)
      
1124. Freezing cell pellets and minimizing lysis - (reply: 2)
      
1125. Additional peaks in cell cycle assay - Can 37°C incubation cause clumping of fixed cells? (reply: 6)
      
1126. Total Cell protein - (reply: 1)
      
1127. Which wash buffer (to collect cell) is prefered for 2DE? - (reply: 3)
      
1128. Mycoplasma - (reply: 1)
      
1129. cell culture media - glutamine! - (reply: 2)
      
1130. How many cells do you have in a confluent T 75 flask? - I have 1/2 million (reply: 2)
      
1131. how to make antigen by trypsin and papain digetion - plz teel me hoe to make antigen by trypsin & papain digetion (reply: 1)
      
1132. can i use trypsin to collect cell sample for 2d gel analysis? - (reply: 3)
      
1133. Premature cell lysis - (reply: 2)
      
1134. urgent!!! weird Growth in cell line - (reply: 8)
      
1135. Shipping of blood cells over long distance - (reply: 1)
      
1136. refrozen cell pellet - (reply: 1)
      
1137. Drug vehicle giving toxicity in cell culture - With increse in vehicle..... toxicity decrese (reply: 4)
      
1138. contamination in cell line...... - (reply: 4)
      
1139. Neuronal Cell Culture Woes - Not even making it out of the gate! (reply: 7)
      
1140. Number of proteins per cell? - One of THESE questions... ;) (reply: 1)
      
1141. MTT with cells growhing in suspension, not adherent - (reply: 3)
      
1142. Cell ELISA - No differences between cells expressing and not, the receptor (reply: 2)
      
1143. growth curve for attached cells - (reply: 1)
      
1144. cells dont attach hard enough to 96 well - (reply: 3)
      
1145. CTLL-2 culture - preventing cell clumping - (reply: 2)
      
1146. Yet another contamination question - SF9 cells (reply: 11)
      
1147. Problems with transfection in human cells - (reply: 3)
      
1148. HepG2 cells culture conditions - (reply: 2)
      
1149. Should I use fully supplymented medium or low serum medium when treat INS1 cells - (reply: 4)
      
1150. T cell proliferation problem - (reply: 3)
      
1151. How to Handle Cells Shipped Internationally in Dry Ice? - (reply: 2)
      
1152. Differences between cells in different species - (reply: 5)
      
1153. Will pervious PBS concentration in medium have a long lasting effect on cell gro - (reply: 2)
      
1154. isotype control for GFP expressing cells? - (reply: 2)
      
1155. Trypsin for J774A Macrophages? - (reply: 2)
      
1156. Trouble with growth of transformed cells - (reply: 4)
      
1157. nuclear extraction of blood cells - (reply: 2)
      
1158. Problems about detection of apoptotic cells using Annexin V/PI staining - (reply: 2)
      
1159. ELISA with undiluted cell lysate samples - (reply: 1)
      
1160. How can you expand cells if you have one dish of them? - (reply: 1)
      
1161. Androgen independent LNCaP cells - (reply: 2)
      
1162. mycoplasma detection kit - (reply: 1)
      
1163. What is the proper way to infect cell culture with virus? - (reply: 5)
      
1164. Micro-RNA profiling for differentiating cells - (reply: 1)
      
1165. Growing insect cells with other cell lines - (reply: 2)
      
1166. Candida albicans cells lysis - method (reply: 1)
      
1167. Cell cycle analysis after viral transduction - Shift of peak (reply: 1)
      
1168. Advise for Whole Cell Stain - hESC-differentiated Trophoblast (reply: 1)
      
1169. 293 T cells - (reply: 3)
      
1170. Mouse Bone Marrow Isolation - How to increase yield of BM-derived cells? (reply: 1)
      
1171. BL-21 Gold (DE3) cells and further strains - (reply: 2)
      
1172. Cell Cycle Analysis flow cytometry - How to measure desynchronisation rate of cells (reply: 1)
      
1173. Simple & straightforward question - Buffering agent for CHO cell medium (reply: 1)
      
1174. Help! Cell homogenization and protein extraction for Western Blotting - (reply: 2)
      
1175. Fixing cells before staining of surface markers - (reply: 1)
      
1176. Black dots in cell culture - (reply: 7)
      
1177. cell counting - (reply: 5)
      
1178. Optimization for cell viability assay (MTT assay) - (reply: 1)
      
1179. Anyone thought about reprogram fibroblast to liver cells? - (reply: 2)
      
1180. cell confluence in cell cultures - myoblast c2c12 in particular (reply: 2)
      
1181. cell line adherence - cells adhering to flask very tightly after transfectiono (reply: 1)
      
1182. Cell labelling - How can I label 2 cell populations? (no transgenes) (reply: 6)
      
1183. How long does it take for cells to reattach? - From balls to pancakes. (reply: 3)
      
1184. Dot plot cell cycle analysis-strange dots - (reply: 4)
      
1185. TERT transformation of fibroblast cells - Immortilising fibroblast cells. Which companies available? (reply: 1)
      
1186. Apoptosis vs Viable Cells vs Necrosis (Annexin/PI Staining) - Which quadrants to sum up? (reply: 4)
      
1187. help Splashed Raji cells on skin/face/hand - (reply: 2)
      
1188. Help! ES Lenti infection efficiency=nightmare - trouble getting over 40 % cells infected (reply: 8)
      
1189. Staining corneal endothelial cells for mechanical damage with methylen blue - (reply: 1)
      
1190. CO2 level for cell culture - (reply: 6)
      
1191. sphere and suspension culture subculturing - (reply: 1)
      
1192. suspension cells for confocal - (reply: 1)
      
1193. RNAi is somatic and germline cells - (reply: 3)
      
1194. Luciferase and cell responsiveness - (reply: 3)
      
1195. adult cell culture - (reply: 1)
      
1196. protein overexpression in HeLa cells - (reply: 1)
      
1197. Insulin supplements to cell culture media - (reply: 1)
      
1198. After inducing with IPTG for protein expression, my cell pellet after centrifuga - (reply: 1)
      
1199. RNA/cell accumulation of difficult-to-obtain tissue - (reply: 1)
      
1200. Bacteria to Cell Culture - How many bacteria should I add to cell culture (reply: 2)
      
1201. topic and cell culture - (reply: 2)
      
1202. Running a western with cell media in gels? - (reply: 11)
      
1203. Starting cells for making one's own competent cells - (reply: 7)
      
1204. cell loss during antibody staining for flow cytometry - FACS staining protocol (reply: 5)
      
1205. Persistent stable cell line (Bos23) Contamination... - (reply: 2)
      
1206. Hematopoietic cells separation - How to get high purity and viability hematopoietic cell populations? (reply: 2)
      
1207. Transfection of large plasmid - How to get a 10kb plasmid into HEK293 cells (reply: 3)
      
1208. min6 cells immunofourescence staining - staining and culture problem (reply: 4)
      
1209. 2D-PAGE of G protein coupled receptor - Need advice on sample preparation - GPCR overexpressed in HEK293 cells (reply: 1)
      
1210. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
      
1211. Rabbit cells to grow bacteria in before immunization of rabbit for antibody prod - (reply: 2)
      
1212. rat ES cells - (reply: 2)
      
1213. Mycoplasma contamination - (reply: 1)
      
1214. Neuronal Cell Culture - Media Changes - How to change media half and half (reply: 4)
      
1215. establishment of primary cell line from biopsy - (reply: 2)
      
1216. Cell culture fasting - (reply: 1)
      
1217. mutated HeLa cells? - from cycloheximide? (reply: 1)
      
1218. Do you get insoluble proteins also in whole cell lysate? - (reply: 3)
      
1219. qPCR for transformed cells - (reply: 1)
      
1220. solution to a weird problem - pH - Adjusting pH in cell culture media (reply: 1)
      
1221. A solution to a weird problem - pH - Adjusting pH in cell culture media (reply: 6)
      
1222. descrepancy between total cell lysate and cell fractionation - (reply: 1)
      
1223. Disruption of cells using mild detergent - (reply: 2)
      
1224. Eruption of mammalian cells using detergent - (reply: 1)
      
1225. lenti virus transfected cell dies... - (reply: 4)
      
1226. Extremely Weird Issue - CHO Cell Culture (reply: 2)
      
1227. Cell Die.. Need help - (reply: 1)
      
1228. Cell scraping ruining my life - (reply: 6)
      
1229. How much trypsin/Cause of death - (reply: 3)
      
1230. Death of cells in the control. - (reply: 1)
      
1231. cell culture clarification - I need help please (reply: 2)
      
1232. Serum concentration in treatment of cells for MTT assay - (reply: 1)
      
1233. Minimum number of cells needed for cytometry? - (reply: 3)
      
1234. Beta-Lactam in CHO Cells - (reply: 1)
      
1235. problems with 293T cells after medium removal!!! - (reply: 6)
      
1236. B cell subset for hybridoma generation - Which B cell subset is used to make hybridomas (reply: 2)
      
1237. Propagating ccdB survival cells - (reply: 6)
      
1238. Western difficulty in primary neuron cells. - (reply: 1)
      
1239. DOubt - Competent cell (reply: 2)
      
1240. Differentiation of CD11b cells into macrophages.Help please - (reply: 1)
      
1241. Please Help!! - Mycoplasma (reply: 5)
      
1242. Primary keratinocyte isolation- cell loss - (reply: 1)
      
1243. cells attach but don't flatten out - MDA-231 (reply: 8)
      
1244. Problem Generating Stable HeLa Cells - (reply: 1)
      
1245. Retroviral transduction of T cells - (reply: 4)
      
1246. Looking for "biocell" from the forum archives. - Cell culture contamination problems.... (reply: 1)
      
1247. p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells - p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells (reply: 9)
      
1248. Multiple nuclei?? - cell morphology - FaDu (reply: 8)
      
1249. Viral RNA is relatively stable than mRNA from cells? - (reply: 1)
      
1250. how to compensate the cell death during transfection by electroporation - (reply: 2)
      
1251. H-ras transformed RK3E/tv-a cells - (reply: 1)
      
1252. HEK001 Cell Line and Papillomavirus - Does HEK001 cell line produce a functional papillomavirus (reply: 4)
      
1253. 2 questions: myeloma cell lines and mice MABs - (reply: 5)
      
1254. I'm getting fried-egg looking cells... What should I do?? - (reply: 8)
      
1255. Empty bubbles in the 3T3-L1 cells - (reply: 3)
      
1256. Exposing Cell Lines to Drugs Metabolized by Liver Microsomes - (reply: 1)
      
1257. UV safety in cell culture hood - (reply: 3)
      
1258. Where can I find info on the physical appearance of TB cell wall structures? - I am an artist wanting to depict the cell wall with absolute accuracy. (reply: 7)
      
1259. intracelluar staining without killing cells - (reply: 3)
      
1260. Info about A7r5 cell culture - (reply: 1)
      
1261. Molecular Cell Biology - Trafficking and function of a membrane enzyme (reply: 1)
      
1262. Cell lysates - Protein precipitation? - (reply: 3)
      
1263. Glucose in epithelial cell - (reply: 1)
      
1264. cell lysate for pulldown - (reply: 3)
      
1265. cell culture media - (reply: 3)
      
1266. Competent cell storage for long term use - (reply: 2)
      
1267. My RIPA cell lysis buffer - Western blot (reply: 3)
      
1268. Cell Uptake Study - (reply: 1)
      
1269. what will happen if my cell culture have exposed to unfiltered air for a few sec - (reply: 2)
      
1270. aspects of RAW264.7 cells - (reply: 5)
      
1271. anti his antibody for western blotting - high background in cell lysate western blotting (reply: 3)
      
1272. Conditioned Medium - Primary skeletal muscle derived cells conditioned medium (reply: 3)
      
1273. Lentivirus-based Knockdown for miRNAs? - for hard-to-transfect cells (reply: 10)
      
1274. Hoechst Stain does it stain dead cells - (reply: 3)
      
1275. Mycoplasma detection - (reply: 2)
      
1276. Help for cell preparation for FACS... - (reply: 1)
      
1277. Cell Lysis Buffer - (reply: 6)
      
1278. HeLa cells and HPV - (reply: 2)
      
1279. Resolving cell growth problems using growth additives - way to resolve urgent cell growth problems (reply: 3)
      
1280. Low transformation efficiency - Rosetta-gami™ 2(DE3)pLysS Competent Cells (reply: 8)
      
1281. problem with MACS cell isolation - (reply: 5)
      
1282. cell culture split ratios. - (reply: 2)
      
1283. MDA-MB-231 cell culture issue - after thawing first passage is fine, next goes bad (reply: 7)
      
1284. detection of mycoplasma with hoechst staining - (reply: 1)
      
1285. blood red cells - (reply: 1)
      
1286. Attention-seeking cell cultures - reduce subculture ratio? - QM7 cells do the darndest things (reply: 2)
      
1287. NFKb signalling in cell line ??? - (reply: 2)
      
1288. kit fpr cell membrane preparation? - (reply: 2)
      
1289. Retrovirus Production - I am not getting any virus from my packaging cells! (reply: 4)
      
1290. How to find NFKb signalling is active in a cell line??? - (reply: 2)
      
1291. Fixing YFP cells - (reply: 2)
      
1292. MTT Assay: cell proliferation or cell viability??? - (reply: 4)
      
1293. Rescue RAW cell culture - RAW cells past confluent point... can they be rescued? (reply: 4)
      
1294. cell cycle arrest by colcemid - (reply: 2)
      
1295. is my lost protein is bcoz of cell death or my treatment worked??? - (reply: 2)
      
1296. CD19 Expression at various stages of plasma cell differentation - (reply: 1)
      
1297. PROLIFERATION ASSAY IN MICROTITER PLATES WITHOUT A SEMIAUTOMATIC CELL HARVESTER - (reply: 1)
      
1298. THP cells? what does THP stands for?????????? - (reply: 1)
      
1299. Immunioprecipitation from Jurkat cells?? - (reply: 1)
      
1300. Weird effect of siRNA on protein in cells - (reply: 3)
      
1301. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
      
1302. EDTA addition to trypsin solution (1x) - (reply: 1)
      
1303. Apoptosis induction - for vero and mcf-7 cells (reply: 5)
      
1304. Need pH 6 Cell culture media - (reply: 1)
      
1305. Are my cells undergoing epigenetic changes? - (reply: 2)
      
1306. establishment of stable cell line using hek293t - (reply: 2)
      
1307. Cell pellet frozen -80 then -20 - (reply: 4)
      
1308. total cell protein vs membrane protein - (reply: 4)
      
1309. tranfection of shRNA into mammalian cells is causing the cells to die - (reply: 4)
      
1310. transfection efficiency - i am looking for a cell line that its transfection efficiency is high (reply: 4)
      
1311. Fixing the cells for FACS - Paraformaldehyde or Formaldehyde (reply: 2)
      
1312. Problem with control samples in cell IC - (reply: 3)
      
1313. Recommendation for cell cycle analysis software needed - (reply: 1)
      
1314. Tips on fermenting DH5alpha and MG1655 - e.coli cells never leave lag phase during fermentation (reply: 1)
      
1315. Cell culture contamination question - what the hell are these things?? - (reply: 3)
      
1316. 'Stringy' contamination of cells? - Weird stringy thing in cell culture flasks (reply: 3)
      
1317. Modified PBS buffer for red blood cells washing - [urgent, need opinions] (reply: 2)
      
1318. Immunofluorescence using dead cells? - (reply: 1)
      
1319. floating THP-1 cells - medium change advice - (reply: 2)
      
1320. my cells love infection, any thoughts on antibiotics? - (reply: 3)
      
1321. Culture Media for stem cells - (reply: 1)
      
1322. Trypsinization or Scraping for adherent cells in ChIP assay? - (reply: 8)
      
1323. Anyone heard of cells spontaneously fluorescing red?? - (reply: 4)
      
1324. Freezing cells - How long can I keep cells at -80C? (reply: 6)
      
1325. Help regarding cell line - (reply: 1)
      
1326. organizing cell lines - (reply: 1)
      
1327. Cell plating density - (reply: 3)
      
1328. Murine antibodies - A panel of antibodies for murine blood cells (reply: 2)
      
1329. transfecting cells with mature miRNA or precursor miRNAs? - (reply: 5)
      
1330. MDA-MB-231 cells question - (reply: 3)
      
1331. thawed cells wont adhere - pc3 and LNcaP (reply: 2)
      
1332. DiFi Cell line (colon carcinome) - Where can I get it???? (reply: 1)
      
1333. HaCaT cells - (reply: 2)
      
1334. lncap and MCF-7become ethanol sensitive? - are lncap cells and MCF-7 cells ethanol-sensitive? (reply: 2)
      
1335. Dying endometrial stromal cells - (reply: 1)
      
1336. Help me - Are my cells infected with something or could this be unsusual morphol - (reply: 5)
      
1337. how to dissociate cells for cell migration assay - (reply: 3)
      
1338. soft agar assay - cells in the top agar cells adhere to the plates unexpectedly. (reply: 4)
      
1339. RNA extraction - how many cells do I need at least?? (reply: 4)
      
1340. RNA extraction protocol for small amounts of cells - (reply: 2)
      
1341. about estrogen receptor beta negtive cell lines - can anyone give me some suggestion about ER beta negtive cell lines? (reply: 1)
      
1342. Problems when harvested cells with SDS loading buffer - extremely sticky & formed clumps (reply: 4)
      
1343. strange morphology of sp2/0 cells after fusion!!! Please help! - morphology switch from round to triangular!!!! (reply: 2)
      
1344. fabrablast primery cell line problem - how to detach cell well (reply: 1)
      
1345. what solvent is better compatible for most cell lines? - DMSO vs methanol (reply: 3)
      
1346. Cell growth rate & O2 consumption - (reply: 2)
      
1347. I did not change the media the day after transfecting 293T cells. Are they going - (reply: 1)
      
1348. Cells stick to dish in Embryoid Bodies - (reply: 1)
      
1349. Changing the media after transfection of 293T cells - I did not change the media the day after transfecting 293T cells. (reply: 7)
      
1350. Non Mouse / Human Cell Line that is easily transfected - (reply: 2)
      
1351. how to plate cells precisely - (reply: 1)
      
1352. RNA extraction from stained cells - (reply: 2)
      
1353. Methods to assess cell viability - (reply: 1)
      
1354. suspension cell transient transfection - (reply: 10)
      
1355. Adherent cells washing off in 96 well -MTT - (reply: 4)
      
1356. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
      
1357. plasmids - plasmids for stable cell line construction (reply: 2)
      
1358. abnormal nuclear morphology after treating shRNA - confocal, shRNA, nuclear morphology, cell division (reply: 2)
      
1359. average total protein content per 293t cell - (reply: 2)
      
1360. T cells preparation for FACS - (reply: 1)
      
1361. counting cells - (reply: 3)
      
1362. Cell OD and spec problems - (reply: 1)
      
1363. cell confluency & effect on cells...?? - (reply: 4)
      
1364. Pipet trypsin tips - (reply: 3)
      
1365. Fixing cells for flow cytometry - alternative to 1% paraformaldehyde (reply: 3)
      
1366. mycoplasma false positive - Luminescence false positives for myco (reply: 2)
      
1367. Washing Red Blood Cells - Tips on washing red blood cells (reply: 8)
      
1368. MEF Cell marker - (reply: 1)
      
1369. (CELL CULTURE) Serious advice needed regarding my career..? - (reply: 2)
      
1370. Change of colour in cell culture media...? - (reply: 2)
      
1371. cell count - how to calculate (reply: 2)
      
1372. generating cell line knockouts - (reply: 3)
      
1373. Cancer Stem Cells?? - (reply: 10)
      
1374. Some kind of contamination? - mouse transduced cell line (reply: 7)
      
1375. Cell stimulation of transcription - (reply: 5)
      
1376. Mouse blood density/osmolarity of blood cells for separation - (reply: 5)
      
1377. suitable cell lysis buffer - (reply: 1)
      
1378. Toluidine blue staining for cell culture - (reply: 1)
      
1379. cell culture - 3D cell culture (reply: 1)
      
1380. Aspergillus in cell culture room - HELP!!! how hardcore to decontami - Cell culture, contamination (reply: 6)
      
1381. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
      
1382. MEF culture contamination? - Contaminated cell culture? (reply: 2)
      
1383. very simple question about hemocytometer cell counting - (reply: 1)
      
1384. nucleic acid extraction from silenced cells - (reply: 6)
      
1385. direct lysis of cell pellet - How to get rid of DNA? (reply: 3)
      
1386. Will mycoplasma contamination lower transfection efficiency? - (reply: 10)
      
1387. In Vitro Drug Exposure Cytotoxicity Testing and Cell Confluency - (reply: 8)
      
1388. Dendritic cells - Protocol of stimulation of dendritic cells (reply: 3)
      
1389. giant cells in HEK293 cell line - (reply: 10)
      
1390. Trypsin/EDTA inactivation - which compound in the medium is responsible for inactivation? (reply: 5)
      
1391. Plant cell transfection - (reply: 1)
      
1392. stable cell line - selection after retroviral infection (reply: 1)
      
1393. Storing cell lines long-term at -70C - (reply: 4)
      
1394. Commercially available primary mouse endothelial cell line? - (reply: 2)
      
1395. Frozen cells in -80 - (reply: 1)
      
1396. Plant or Fungal Cell Dissociation Technique? - (reply: 3)
      
1397. how can i bring cells onto a slide? - ...for microscopical observation! (reply: 7)
      
1398. cell density to be seeded? - cytotoxicity studies (reply: 1)
      
1399. Lysis of the cells - (reply: 1)
      
1400. Cells do not lyse in SDS buffer - (reply: 1)
      
1401. Storage of Fixed Cells for Immunofluorescence staining - (reply: 1)
      
1402. lysis buffer for lymphoblast cells - (reply: 1)
      
1403. problem with cell cycle analysis - (reply: 3)
      
1404. THP-1 Cells differentiation - THP-1 cells differentiated using IFN-gamma/LPS (reply: 7)
      
1405. Single cell suspension from solid tumor - (reply: 3)
      
1406. cell aggregation? - (reply: 1)
      
1407. T-Cell receptor - Is it a single type on a T cell or multiple types? (reply: 1)
      
1408. cells weak adhesion - detachment (reply: 4)
      
1409. how we count the passages in cell cultures? - (reply: 5)
      
1410. resolving cell growth problems - (reply: 2)
      
1411. clumping of cells - (reply: 7)
      
1412. stability of cell suspension - (reply: 1)
      
1413. cell adhesion in serum free condition - (reply: 1)
      
1414. effects of cell confluency - (reply: 1)
      
1415. Which trypsin EDTA to use? - (reply: 3)
      
1416. Processing cell samples for low abundance proteins - direct lysis with SDS-PAGE loading buffer (reply: 2)
      
1417. Good Cell line for Histone 3 and 4 - Positive Control for Basic Staining and WBs (reply: 1)
      
1418. polarized cells - (reply: 4)
      
1419. PCNA (proliferating cell nuclear antigen) - is it an adequate marker of proliferating cells? (reply: 2)
      
1420. cell adhesion assay - using collagen (reply: 1)
      
1421. Problem clariflying/filtering insect cell lysate - (reply: 2)
      
1422. cell culture urgent!! - (reply: 2)
      
1423. Moving S2 cells from one plate to another - (reply: 1)
      
1424. Cell culture medium instead of Nucleofector solution?? - (reply: 3)
      
1425. Nucleofection of ES cells, medium instead of nucleofector solution? - (reply: 1)
      
1426. mononuclear cells medium - I am stuck with the concentration of 2-mercaptoethanol (reply: 1)
      
1427. Accidental thawing of frozen cells - (reply: 3)
      
1428. how to start with a new cell line ? - how to start with a new cell line ? (reply: 1)
      
1429. HaCaT cells die after trypsinization - (reply: 1)
      
1430. Transporting some bacteria cells - transport bacteria cells pellet (reply: 2)
      
1431. GFP and Luciferase Cell Line - (reply: 2)
      
1432. Transfecting primary cells with plasmid - (reply: 1)
      
1433. transfecting and selecting EL4 cells - (reply: 1)
      
1434. Induce Osteoblast lineage from C2C12 myoblast cells - (reply: 1)
      
1435. Lentivirus production with LNCX vector - Do I have to use Packaging cell lines? (reply: 3)
      
1436. Issue on mycoplasma detection and treatment in cell culture - (reply: 3)
      
1437. Transfection into eukaryotic cells - (reply: 1)
      
1438. Fixing cells with PFA - (reply: 1)
      
1439. A549 cells not doubling - has anyone been through this with other cell lines? (reply: 4)
      
1440. Contamination in SH-SY5Y cultures, mycoplasma? - (reply: 2)
      
1441. Immunofluorescence in HEK cells issues - (reply: 1)
      
1442. cell sorting - losing cells? - (reply: 3)
      
1443. What if my expressed protein is toxic for the cells... - (reply: 1)
      
1444. competent cells - difference between electroporation and chemical comp. cells (reply: 1)
      
1445. Possible contamination of leukemic cell culture - debris, growth inhibition, cell aggregates (reply: 3)
      
1446. human cancer cell line splashed into my eyes - (reply: 3)
      
1447. Saving tissue and single cell suspension - FACS, single cell suspension (reply: 1)
      
1448. BL21(DE3)LysS competent cells - (reply: 1)
      
1449. ATP turnover in neuron - ATP hydrolysis rates in neuronal cells (reply: 2)
      
1450. Adhering CHO cell culture - cells don't adhere! (reply: 2)
      
1451. do e.coli cells get ruptured during high spin - (reply: 1)
      
1452. Please Help with COS-1 cells^^ - (reply: 1)
      
1453. antibiotics for preventation of mycoplasma contamination - (reply: 4)
      
1454. IPTG Induction and cell lysis issues - pET 21b system (reply: 1)
      
1455. cell counting - to get an impression when my cells are in the log phase (reply: 1)
      
1456. Whole cell lysis of fibroblasts - (reply: 1)
      
1457. Whole cell lysis of fibroblasts - (reply: 1)
      
1458. test of cytotoxic effect of compounds - why cells are pretreated with actinomycin C ?? (reply: 1)
      
1459. Proliferation Assay - Should cells be in the logarithmic phase ? (reply: 3)
      
1460. cell density and growth - (reply: 5)
      
1461. Laser capture microdissection - capturing single cells (reply: 2)
      
1462. when cells have to be synchronized?? - (reply: 2)
      
1463. cells in RNAlater - the title say`s it all :) (reply: 4)
      
1464. Cytotoxicity assay for adherent cells exposed to particles - (reply: 1)
      
1465. Murine Cell Lines - (reply: 2)
      
1466. Cell Proliferation: To adhere or not to adhere (before treatment), that is the q - (reply: 2)
      
1467. why are some cell lines more popular than others?? - (reply: 4)
      
1468. NK cell staining - (reply: 4)
      
1469. cell culture media - with or w/o L-glutamine? (reply: 2)
      
1470. Measuring DNA, RNA, protein and cell wall synthesis - (reply: 1)
      
1471. pro-apoptotic effect of compounds on cancer cell lines - which cell line to test the non-toxic effect? (reply: 3)
      
1472. Expiry of Trypan blue - (reply: 3)
      
1473. Question in Generating double stable cell line - (reply: 3)
      
1474. activity of sex-chromosome in somatic cells - (reply: 5)
      
1475. CaCl2 for making competent cells - (reply: 3)
      
1476. Calcium Phosphate Transfection for CV1 CELLS - (reply: 2)
      
1477. ICC Cell Adherence Issue - (reply: 1)
      
1478. gentamycin resistance in human cencer cell culture - please suggest the alternative antibiotic (reply: 2)
      
1479. siRNA control (scramble seq) causing cell death - what to try next? (reply: 2)
      
1480. How to normalize my samples before I transfect my cells - (reply: 6)
      
1481. Isolation of intestinal epithelial cells - Isolation of intestinal epithelial cells (reply: 2)
      
1482. Number of proteins expressed in cell - (reply: 2)
      
1483. Do HEK 293 cells contain functional E1A/E1B? - (reply: 2)
      
1484. Sodium Pyruvate - What is it's use in (NIH 3T3) cell culture? (reply: 4)
      
1485. Calcium phosphate method vs lipofectamine - troublemaker neuroblastoma cells (reply: 7)
      
1486. Which cell lines are best for transient transgene expression? - (reply: 3)
      
1487. Alzheimer's cell model - (reply: 7)
      
1488. Partial cell subculture? - (reply: 2)
      
1489. No difference between transfected and untransfected cells - Using eGFP siRNA on GFPHEK293 cells usign flurimeter (reply: 5)
      
1490. Whole Kidney primary cell culture - (reply: 1)
      
1491. dh5 alpha competent cells? - (reply: 2)
      
1492. which is the best lysis buffer for whole cell extracts? - (reply: 1)
      
1493. Please help me to understand- cell counting - (reply: 1)
      
1494. Stimulating hepatocytes with IL-1beta - (reply: 1)
      
1495. Preparing competent cells under laminar flow? - or on the bench sufficient? (reply: 5)
      
1496. Cell count - Please help - (reply: 11)
      
1497. What is DME medium for Hybridoma cell culture - is DME medium and DMEM medium are the same for hybridoma culture?? (reply: 1)
      
1498. Cell Count - (reply: 1)
      
1499. Generating stable cell line - (reply: 2)
      
1500. Harvesting cells off of collagen gels - (reply: 1)
      
1501. cells can't be lysed after crosslinking - (reply: 4)
      
1502. How many cells to seed? - simple question :) (reply: 5)
      
1503. Is there a problem by adding HCL to the cell culture medium in order to manipula - (reply: 1)
      
1504. mouse macrophage cell line - (reply: 5)
      
1505. Problem clarifying lysate from Hi5 insect cells - (reply: 1)
      
1506. cell line information - require (reply: 1)
      
1507. Urgent problem - Media change = cells die in 48h (reply: 3)
      
1508. recombineering for ES cell targeting - retrieval problems (reply: 1)
      
1509. Rat monocyte cell line - (reply: 1)
      
1510. How often do you thaw new cells? - passage number or time? (reply: 7)
      
1511. Does high speed centrifugation destroy viable cells? - (reply: 2)
      
1512. Cell culture - (reply: 7)
      
1513. Problems when plating neuroblastoma cells - (reply: 3)
      
1514. Is pFLAG expression toxic? - FLAG seems to kill my HeLa cells. This can't be right (reply: 1)
      
1515. Cell Doubling Time - (reply: 3)
      
1516. Dimethyl Sulfate (DMS) as carrier in cell cultures - (reply: 1)
      
1517. Amount of fixation buffer - is it dependent on the number of cells? (reply: 2)
      
1518. synchronized cells or non-synchronized cells - (reply: 3)
      
1519. pre-prepararation of competent cells - (reply: 3)
      
1520. ROS accumulation - ROS reactive oxygen species accumulation in cells (reply: 3)
      
1521. Bacterial invasion assay with human cells - (reply: 2)
      
1522. Measuring gene expression across cell lines - (reply: 2)
      
1523. trypan blue without hemocytometer? - (reply: 2)
      
1524. How to make an isotonic solution for h9c2 cells - (reply: 2)
      
1525. migration < invasion??? - More cells migrating through matrigel than through control inserts (reply: 1)
      
1526. Zeocin of G418. Which is the better selection agent for CHO cells? - (reply: 1)
      
1527. Immunofluorescence with transfected cells - (reply: 3)
      
1528. Cloning Problem (cells grow but no plasmid isolated) - (reply: 13)
      
1529. Problem: protein precipitates from cell lysate after defrosting - (reply: 4)
      
1530. Contamination in cell cultures - (reply: 9)
      
1531. DMSO killing more cells at lower concentrations - (reply: 1)
      
1532. frozen competent cells - how to prepare competent cells from frozen bacteria? (reply: 3)
      
1533. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
      
1534. lysing cells on ice - (reply: 2)
      
1535. Fibroblast cell culture - (reply: 1)
      
1536. Cardiac stem cells - (reply: 3)
      
1537. How to visualize tumor cells in mice with out sacrificing the animals - (reply: 3)
      
1538. cell count and dilution - (reply: 1)
      
1539. z competent cells - (reply: 2)
      
1540. immunofluorescence staining of sperm cells - (reply: 2)
      
1541. Cell Culture procedure(manual, protocole, what ever ;) - (reply: 1)
      
1542. colony formation assay - how many cells to seed (reply: 2)
      
1543. Primaria/gelatin plates and gravity-defying fixed cells - (reply: 2)
      
1544. Contaminations in cells cultured in suspension - (reply: 2)
      
1545. suspension cell transient transfection - (reply: 2)
      
1546. What is this ? - Cell culture contaminant (reply: 2)
      
1547. cell cryopreservation after siRNA transfection - (reply: 1)
      
1548. Cell Digestion Protocol - (reply: 1)
      
1549. spin hek cell lysate after adding laemmeli buffer? - (reply: 1)
      
1550. cell growth - (reply: 4)
      
1551. primary cells could not attch to regular polystyrene flasks but immortalised cel - does anybody know what could be happening (reply: 1)
      
1552. How to culture cells after sorting? - (reply: 3)
      
1553. How to kill nerve cells in a living hydrozoans? - Trying to procude partial/complete nerveless jellyfish (reply: 3)
      
1554. lifespan of primary cells in vitro - how long can different cell types survive (reply: 3)
      
1555. measuring apoptosis - reduced cell proliferation due to apoptosis (reply: 1)
      
1556. T cell differentiation protocols - Th1/Th2/Treg/Th17 (reply: 4)
      
1557. Mitochondria extraction from human liver cells - (reply: 1)
      
1558. lipofectamine and PEI transfection of hela cells - (reply: 2)
      
1559. What can I do whit my Cancer Stem Cells?(I know, its a big question..) - (reply: 7)
      
1560. Wash L929 Cells with DPBS (with or w/out CA MG?) - DPBS with or W/out CA,MG? (reply: 2)
      
1561. Especially tenacious cells -- what to do? - (reply: 9)
      
1562. MDCK cell vascuolation - I got my MDCK cell growing in the T-75 flask (reply: 5)
      
1563. Culturing HeLa Cells - (reply: 2)
      
1564. expression of His-tagged protein in insect cells - (reply: 2)
      
1565. How to isolate vascular smooth muscle cell? - (reply: 1)
      
1566. Whole cell lysate including nucelar proteins? - (reply: 3)
      
1567. Colony PCR with Eukaryotic Cells? - Any Experiences? (reply: 4)
      
1568. HELP with cell lysis for DNA assay - Something is floating in my lysate solution!!! (reply: 1)
      
1569. culturing MCF-7 cellls - clumping and maintaining healthy cells (reply: 3)
      
1570. Cell culture media - require information on media (reply: 3)
      
1571. how to measure ROS in cell wiht H2DCFH - (reply: 4)
      
1572. VEGF in cell nucleus - (reply: 2)
      
1573. cell treatment rules - (reply: 4)
      
1574. method to seed cells in hyrogels - cell seed in hydrogels (reply: 1)
      
1575. transfecting human mesynchemal stem cells - (reply: 5)
      
1576. cell schock - (reply: 2)
      
1577. Flow cytometry of RFP and CFP expressing yeast cells - (reply: 1)
      
1578. Can you store cell pellets for future nuclear extraction? - (reply: 5)
      
1579. using cell lysate to run native gel? - (reply: 1)
      
1580. Cell Suspension from Frozen Tissue? - (reply: 6)
      
1581. looking for stromal cell marker - (reply: 1)
      
1582. 'Black dots' in cell culture - (reply: 2)
      
1583. ChIP in WT and KO cells - how to analyze - (reply: 1)
      
1584. Mouse T cell line - (reply: 1)
      
1585. Making competent cell from freeze-thawed stock - (reply: 2)
      
1586. Invasion assay using matrigel- cell clumps - (reply: 1)
      
1587. producing cell lines - why viruses ?? (reply: 4)
      
1588. What color should the cell pellets be after sonication and centrifugation? - (reply: 1)
      
1589. Please help--possible bacteria issue--cholesterol + red blood cells involved - (reply: 1)
      
1590. Mycoplasma Question - (reply: 2)
      
1591. Cell Lysis for DNA extraction - Not every sample is successful (reply: 5)
      
1592. How to detach an antibody from cell surface - (reply: 4)
      
1593. Water for electrocompetent cells - (reply: 2)
      
1594. Gp 293 Luc cells - (reply: 1)
      
1595. how to check cell viability - (reply: 1)
      
1596. Starter culture dilution (preparation of competent cells) - I don't know how much should I dilute my starter culture... (reply: 3)
      
1597. cell fixation - (reply: 1)
      
1598. cell detachment in IHC - can I incubate cells with hoechst, heparin and PFA at the same time? (reply: 1)
      
1599. cell immobilization for confocal - (reply: 1)
      
1600. problem with 293T cell co-transfection - (reply: 5)
      
1601. Transfection and WB - Transfected cell line shows incorrect bands on WB (reply: 2)
      
1602. Cell Death after formaldehyde treatment - Immunofluorescence (reply: 5)
      
1603. Cell Densitometry - (reply: 1)
      
1604. My cells died after changing media... - (reply: 6)
      
1605. Help for an Organic Chemist - Question about bacteria cell wall permeability. (reply: 6)
      
1606. H9C2 cells - trouble when culturing H9C2 cells (reply: 4)
      
1607. Contamination of cell ?? - (reply: 2)
      
1608. Stem cells, primary culture - Literature - What are some good papers to read to get started in this area? (reply: 3)
      
1609. nonspecific band in transfected c-abl cells - nonspecific band in transfected c-abl cells (reply: 1)
      
1610. Passaging Cells - (reply: 4)
      
1611. Modifying collagen stiffness / rigidity in 3d cell culture - (reply: 1)
      
1612. Mysterious cell death - need Hela Help! - (reply: 1)
      
1613. NSC-34 Cells sloughing off at media change - (reply: 4)
      
1614. Chosing the right competent cells - (reply: 1)
      
1615. Cell proliferation assay for LNCaP cells - (reply: 4)
      
1616. liquid nitrogen in cell culture - (reply: 2)
      
1617. differential cell counting in mixed cultures - (reply: 1)
      
1618. Questions about animal cell cultue Please answer - (reply: 1)
      
1619. immunofluorescence for HUVEC - how to do immunofluorescence for cancer cells (reply: 3)
      
1620. membrane type of cell culture filtration - (reply: 3)
      
1621. Critical column of media above cells for access of oxygen - (reply: 1)
      
1622. EDTA bacterial cell lysate - (reply: 2)
      
1623. Is MTS Assay suitable for Adherent cells? - Confused about MTT Assay and MTS assay.. (reply: 1)
      
1624. membrane damaged = cell death? - (reply: 2)
      
1625. 293 cells help! - (reply: 1)
      
1626. stable cell line - (reply: 2)
      
1627. Color of cryopreserved cell line - (reply: 1)
      
1628. Established (tumor) cell line - or not...? - (reply: 3)
      
1629. Culturing cell in absence of any growth factors - Cell culture minus growth factors (reply: 1)
      
1630. proliferation assay for small cell population - (reply: 2)
      
1631. Cell fixation with glutaraldehyde - (reply: 3)
      
1632. Yeast contamination in cell culture - hood to blame? - (reply: 6)
      
1633. Comparing cell # or DNA content - of pelleted cells (reply: 1)
      
1634. bicarbonate in cell culture: powder v. liquid? - (reply: 3)
      
1635. cell adhesion assay - collagen (reply: 1)
      
1636. To break cell clumps - suspension culture related (reply: 4)
      
1637. Problems for MTT in SH-SY5Y cell line - (reply: 1)
      
1638. cell stainer size - (reply: 2)
      
1639. FUNGI SILENCING - PROTOCOL TO TRANSFORM FUNGI CELLS (reply: 2)
      
1640. Difficulties with the transfection of cells CHO DG44 - (reply: 9)
      
1641. Low concentration of FBS inhibiting trypsin? - (reply: 1)
      
1642. contamination in establishing stable cell lines - (reply: 1)
      
1643. How much cell medium for 1 week of culturing? - (reply: 1)
      
1644. p53 +/- matched-pair tumor cell lines? - (reply: 2)
      
1645. Tranfection cell density and overgrowth - (reply: 3)
      
1646. shRNA selection in suspension cell culture - (reply: 6)
      
1647. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
      
1648. Ethanol/methanol/dmso for cell culture - (reply: 1)
      
1649. stored cytokines from supernatant cell culture - stored cytokines from supernatant cell culture (reply: 3)
      
1650. Rapid Cell Count (in vivo) - (reply: 5)
      
1651. cell migration assay (corning transwell) - (reply: 2)
      
1652. cell culture in 2 stack flask - (reply: 1)
      
1653. Invitogen cell countess - (reply: 5)
      
1654. Hypoxia Treatment For Cell Culture - (reply: 4)
      
1655. Picking strain for e coli competent cells - (reply: 6)
      
1656. cell death - can anybody tell me what process this looks like (reply: 3)
      
1657. Liver cancer cell transfection via lipofectamine - Liver cancer cell transfection method (reply: 3)
      
1658. Marker do discriminate cell cycle phases - G2 phase / Mitosis (reply: 3)
      
1659. hematopoietic cell lines - (reply: 3)
      
1660. Cos7 Cells to produce retrovirus - (reply: 2)
      
1661. how to make nonadhensive cells attach to coverslips without affecting cellular s - (reply: 1)
      
1662. Constructing puromycin kill curve - How to count the number of adherent cells without detaching them? (reply: 3)
      
1663. stable cell line generation - (reply: 5)
      
1664. histological staining for plant cell wall components - (reply: 1)
      
1665. My adherent cells are detaching - (reply: 2)
      
1666. Cell culture media - DMEM and DMEM/F-12 (reply: 1)
      
1667. Cell Cycle analysis of proliferating lymphocytes - (reply: 1)
      
1668. Problems with HEK293 cells in 96 well plate format - 293 cells do not adhere well to the plate (reply: 1)
      
1669. Cell Synchronization - (reply: 1)
      
1670. Slow cell culture growth? - (reply: 3)
      
1671. Protein quantification from paraformaldehyde-fixed cells - (reply: 2)
      
1672. cell lysis buffer - (reply: 1)
      
1673. cell aging - cell culture (reply: 2)
      
1674. cell concentrations - (reply: 4)
      
1675. Floating cells...pls help - (reply: 6)
      
1676. Cell migration assay with 3 cell types - (reply: 1)
      
1677. Whole Genome Methylation Analysis Feasibility - Methylation analysis on DNA from rare cell populations (reply: 3)
      
1678. How many B cells do I need to get a 1mg of protien - (reply: 2)
      
1679. Do histology staningings work in cell culture? - (reply: 2)
      
1680. Stimulating/Drugging Mammilian Cells - What is the proper way to add drugs or stimulants to a cell culture? (reply: 3)
      
1681. Trypsin Activity Assay - No Change (reply: 4)
      
1682. T cell transduction - (reply: 3)
      
1683. compound that block DNA replication in human cell - (reply: 1)
      
1684. Tranfection of HL 60 cells - (reply: 1)
      
1685. mouse liver cell line? - (reply: 1)
      
1686. Failed Transformation of Tuner (DE3) cells - (reply: 1)
      
1687. Cells suddenly died after changing the medium... why? - (reply: 4)
      
1688. type of rat hepatic cell for toxicology study - seeking opinion on what type of cell to be used (reply: 1)
      
1689. Food colouring agar plates - Do you think it will affect cells? (reply: 11)
      
1690. CHO Cell contamination - (reply: 6)
      
1691. MDCK cells vacuolation - Vacuolation of MDCK cells while staining (reply: 4)
      
1692. seperating cells and antibodies - Separating cells and antibodies using centrifugation (reply: 1)
      
1693. Neuronal precursor cells - (reply: 1)
      
1694. Sticky cells - (reply: 2)
      
1695. Having problem establishing stable cell line...... - (reply: 5)
      
1696. Transferring mamallian cell colonies for selection - (reply: 2)
      
1697. Antibody titration and Cell number - (reply: 1)
      
1698. Cell Invasion Assay - possible to analyze with flow cytometry? (reply: 5)
      
1699. Cell culture contamination - "outsider I.D." (reply: 2)
      
1700. Alternative media for PC-12 cells - (reply: 2)
      
1701. viability of adherent cells - (reply: 3)
      
1702. How long can viably frozen cells stay good in -80 degree? - (reply: 5)
      
1703. Trypsin the cell killer - How much death does trypsin actually cause (reply: 2)
      
1704. How to grow cell in the iron-deficient environment? - 4T1 cell. (reply: 1)
      
1705. HeLa cells and BCL 2 expression - (reply: 1)
      
1706. B104 cells - (reply: 2)
      
1707. SH-SYS5 cell culture for dummies... - (reply: 4)
      
1708. collagen staining in cell culture - (reply: 5)
      
1709. Hematopoietic lineage depletion kit - Hematopoietic stem cell enrichment kits (reply: 4)
      
1710. changing cell shape - (reply: 2)
      
1711. how to change cell shape? - (reply: 3)
      
1712. About T-antigen and tumor cell lines - (reply: 2)
      
1713. Sub-culture of N2a cells - (reply: 1)
      
1714. Haematopoietic stem cells - (reply: 5)
      
1715. CHO cell line - To use or not to use - - incubator ran out of CO2 (reply: 1)
      
1716. proteomics - minimum cell number (reply: 1)
      
1717. Standard Curve for OD Turbidity vs Cell density - (reply: 6)
      
1718. Cells to Ct RNA isolation kit (Ambion) - (reply: 1)
      
1719. Plasmids for protein expression in mammalian cells - (reply: 2)
      
1720. cell lysis after induction - (reply: 1)
      
1721. competent cell preparation? - (reply: 1)
      
1722. One Shot® ccdB Survival™-T1R Chemically Competent Cells: Gateway cloning???? - problems using competent cells for destination vectors in gateway clon (reply: 5)
      
1723. Dendritic cell infection - (reply: 2)
      
1724. Competent Cells - (reply: 5)
      
1725. looking for bovine skeletal muscle cells - (reply: 5)
      
1726. my cell line doesnt change the colour of the media - even when cells are 100 % confluent (reply: 4)
      
1727. Dead Dendritic cells ... please help - (reply: 1)
      
1728. Cell concentration Question - (reply: 3)
      
1729. cho cell line adoptation to serum free medium - (reply: 1)
      
1730. Problems with viability of Jurkat's cells - (reply: 3)
      
1731. THP-1 cell surface markers for Flow Cytometry - Need a robust marker for undifferentiated THP-1 cells (reply: 1)
      
1732. 24 well HeLa Cell Total Protein Concentration? - What would it be (reply: 2)
      
1733. Gene expressed in lenti-packaging cells, but not in lenti-infected cells - (reply: 2)
      
1734. Culturing U2OS cells - (reply: 1)
      
1735. staph cell count - (reply: 5)
      
1736. What kind of speed for HepG2 cells. - (reply: 2)
      
1737. Serum free culture media 293T cells? - (reply: 1)
      
1738. Embryoid bodies - How to differentiate mouse ES cells quickly and efficiently (reply: 8)
      
1739. cell count - is it right? (reply: 3)
      
1740. Haemocytometer cell count - Coverslips (reply: 4)
      
1741. Growing two cell types in the same flask?? - (reply: 1)
      
1742. Cell isolation - (reply: 1)
      
1743. trouble to grow cells on top of coverslip - (reply: 6)
      
1744. Immortalized murine hepatocytes - (reply: 1)
      
1745. about measuring luciferase activity in living cells - (reply: 9)
      
1746. Culture conditions for cell treatments - (reply: 4)
      
1747. preadipocyte 3T3-L1 cells question? - (reply: 1)
      
1748. SH-SY5Y cell culture - Suspended cells (reply: 4)
      
1749. Magnetic-activated cell sorting ? - (reply: 4)
      
1750. mRNA level in transgene cell line or knocking down cell line - (reply: 3)
      
1751. How to increase IF signal in acetone/methanol fixed cells? - (reply: 5)
      
1752. Macrophage cell culture - Strange particles persist in an otherwise healty looking MF culture (reply: 5)
      
1753. sheep red blood cell rosetting protocol - (reply: 1)
      
1754. Tranformation cell harvesting - (reply: 5)
      
1755. self-renewal Vs proliferation - stem cells (reply: 3)
      
1756. Plyse cell and DH5a - (reply: 1)
      
1757. Brain tumour tissue enzymatic digestion for culturing cells - (reply: 2)
      
1758. INS-1 cell line - (reply: 1)
      
1759. Central cell seeding - (reply: 4)
      
1760. please help with Freezing primary cells - (reply: 1)
      
1761. COMPETENT CELL PREPARATION - (reply: 6)
      
1762. MCF-7 cells dying after siRNA transfection + estrogen - (reply: 1)
      
1763. suggest cells expressing low levels of adhesion molecules - (reply: 1)
      
1764. Cell Culture and Pipette Tips - (reply: 4)
      
1765. Flow cytometry CD14 staining very dim - Bone marrow cells CD14 staining for flow cytometry (reply: 3)
      
1766. Cell counting issues - the ring of death on my flasks (reply: 5)
      
1767. Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
      
1768. Which cell line culture has endogenous Wnt1 expression? - (reply: 2)
      
1769. RNA Isolation - RNA isolation from human cell lines (reply: 2)
      
1770. Cell Fixation - methanol fixation of cells (reply: 3)
      
1771. How to retrieve my human blood immune cells from liquid nitrogen without killing - (reply: 3)
      
1772. stem cells and EB (Embryoid bodies) - (reply: 1)
      
1773. Immunohistchemistry of red blood cells - (reply: 1)
      
1774. cell cryosection - (reply: 5)
      
1775. Shifting of entire cell cycle to the left - (reply: 3)
      
1776. freezing suspended cells - (reply: 7)
      
1777. Cell Brakeage...how will I know - I need the mitochondria. (reply: 1)
      
1778. How many cells... - ...gives you how much RNA? (reply: 2)
      
1779. Cell culture - Culture medium (reply: 1)
      
1780. Anybody ever use whole blood plasma serum stimulate cells? - (reply: 2)
      
1781. How can I separate my cell line from feeder cells - (reply: 3)
      
1782. ChIP-seq: Minimum number of cells - (reply: 3)
      
1783. CHO or VERO? - Cell culture (reply: 2)
      
1784. Media for squamous cells - (reply: 3)
      
1785. how to trace single cell movement throughout gastrulation of zebrafish - (reply: 4)
      
1786. Oxygen diffusion through cell culture medium - (reply: 2)
      
1787. Request for Mono Mac-1 cell line - (reply: 1)
      
1788. help: bovine Intestinal Epithelial Cell Culture protocol - (reply: 4)
      
1789. How much sonication power needed? - protein extraction from cells (reply: 2)
      
1790. protein extraction - from cell line in T25 flask (reply: 5)
      
1791. Getting cells from flasks - (reply: 5)
      
1792. transformation efficiency decreased - storage conditions of competent cells (reply: 7)
      
1793. Fixation of immune cells - "how to" protocol needed (reply: 2)
      
1794. cell cycle analysis by FACS - (reply: 2)
      
1795. Variable expression of antigen in various subcellular locations - Any ideas why? - ICC Staining on Glioma Cells (reply: 2)
      
1796. Trypsin Expression and Purification - (reply: 3)
      
1797. cell cycle analysis PI staining shift - (reply: 1)
      
1798. Lipofectamine transfections once working now dying - Optimised transfection in HEKs starting to cause cell death (reply: 6)
      
1799. Problem with stable overexpression cell lines - (reply: 3)
      
1800. seeking help in stable cell line generation - (reply: 3)
      
1801. SDS, Cell numbers, dilution - (reply: 18)
      
1802. Help with Hep G2 cells - (reply: 1)
      
1803. Floating cells in adherent VERO culture - contamination? (reply: 2)
      
1804. RAW264.7 cells+nitric oxide determination - (reply: 13)
      
1805. BL 21 cells - (reply: 4)
      
1806. Mutated MDCK cells - protocol for mutating MDCK cells (reply: 1)
      
1807. Preparing mutated MDCK cells - method of mutation of MDCK cells (reply: 1)
      
1808. Cell pellet pesuspension without Vortexing or pippeting - seeking advice - (reply: 2)
      
1809. western blot problem: cell culture VS mouse tissue - (reply: 4)
      
1810. How long are cells viable in PBS? - (to make trypan blue exclusion assay less tedious) (reply: 3)
      
1811. Detachment of very adherent cells for flow cytometry - (reply: 7)
      
1812. THP-1-Blue-CD14 cells - (reply: 4)
      
1813. are cells prone to swelling in PBS after sorting - (reply: 3)
      
1814. Is it Possible - Is it possible RNAi cross-reaction with normal cells molecules?? (reply: 1)
      
1815. suspension cell culture - optimum cell number in plates (reply: 2)
      
1816. Whole tissue starting product - not cultured cells - (reply: 2)
      
1817. DNA extraction fron HSV-1 infected Vero cell - (reply: 2)
      
1818. How fast do DH5-alpha cells with plasmid insert lyse after defreezing? - (reply: 1)
      
1819. Overnight SURE cell culture in LB-carbenecillin - (reply: 4)
      
1820. culturing COS-7 cells - (reply: 5)
      
1821. Is gentamycin treatment an option? - Mycoplasma contamination (reply: 4)
      
1822. Would transfected plasmids bind histones in HeLa cells? - h3k4 demethylase LSD1 (reply: 3)
      
1823. Loss of cell adherence after transfection - The first passage after selection process cells lost adherence (reply: 2)
      
1824. how will i be able to culture tumor cells? - (reply: 3)
      
1825. General cell lysis question - whole cell lysates need to be centrifuged? (reply: 2)
      
1826. Rnase for cell cycle - (reply: 5)
      
1827. Transfection Issue - Are MCF-7 & MDA-MB-231 cells generally difficult to transfect? (reply: 2)
      
1828. cell loss upon centrifugatino - (reply: 10)
      
1829. cell lines vs tissue expression - (reply: 1)
      
1830. Is molecular biology grade DMSO good enough for cell stock? - (reply: 2)
      
1831. Clonogenic assay with agressive cell line - (reply: 4)
      
1832. e coli cell lysis - heterologous gene expression in e coli (reply: 3)
      
1833. Number of cells in 6 wells-plate well - (reply: 1)
      
1834. protein extraction from primary cells - (reply: 1)
      
1835. Extracting proteins from cells - (reply: 1)
      
1836. Minimal amounts of whole cell extracts for Co-IP - (reply: 1)
      
1837. strange dots in cell culture flask?? - U87 cell line culture has strange white dots (reply: 6)
      
1838. cell floating in 96-well plate - (reply: 1)
      
1839. Inducing Adherence in Suspension Cells - (reply: 1)
      
1840. Isolated nuclei and trypan blue - Silly question .......... (reply: 2)
      
1841. bacterial contamination in cell culture - (reply: 3)
      
1842. THP-1 cell culture - (reply: 1)
      
1843. H9c2 cell contamination? - (reply: 4)
      
1844. Recovery plasmids from dead bacteria - How can i rescue my plasmid from dead cells??? (reply: 4)
      
1845. Inducible expression in human cell line - suggestions for a good system? (reply: 4)
      
1846. What stains red in trypan blue - (reply: 2)
      
1847. transfection in floating cells - (reply: 5)
      
1848. immortal or stable cells - primary human cells to immortal cell line? (reply: 3)
      
1849. SDS-PAGE Cell lysis problems - (reply: 3)
      
1850. Storage of invitrogen top10 cells - (reply: 11)
      
1851. oxidative stress - how to measure oxidative stress in cells (reply: 2)
      
1852. Cells clots forming during preparation mouse splenocytes - (reply: 4)
      
1853. Transwell Cell Migration/Invasion Assay - (reply: 4)
      
1854. neuronal nitric oxide synthase/cell line - (reply: 3)
      
1855. HL 60 cells passage - (reply: 1)
      
1856. Establishing a new cell line - (reply: 1)
      
1857. Isolating Natural Killer cells from whole blood - (reply: 2)
      
1858. Centrifugation of epithelial cells - (reply: 2)
      
1859. cell engineering exam - (reply: 5)
      
1860. Cell detachment: alternative to trypsine/EDTA? - (reply: 3)
      
1861. Cell supplier - (reply: 2)
      
1862. Adherent cells versus suspension cells - how can i convert adherent cell culture to suspension culture (reply: 6)
      
1863. Mir34a positive control cells - (reply: 4)
      
1864. Medium for MDCK cells overexpressing MRP-1 - (reply: 1)
      
1865. lentiviral transdcution of hematopoëtic stem cells - (reply: 2)
      
1866. FACS positive cells in negative controls help - (reply: 1)
      
1867. Protein Removal from cell lysate - leaving RNA intact! (reply: 1)
      
1868. How to separate fungus cells from human lung tissues - (reply: 7)
      
1869. Growing of TOP10 chemically competent cells - (reply: 4)
      
1870. Stable Cell Selection with Neomycin - (reply: 4)
      
1871. endogenous miRNA of cos-7 cell - (reply: 2)
      
1872. Qustion about MDA-MB-157 cells culture - (reply: 4)
      
1873. Folic acid solution for cell culture - (reply: 2)
      
1874. Mycoplasma contamination - (reply: 6)
      
1875. Strange dots around the cell+Photo - (reply: 6)
      
1876. FACS of GFP expressing cells from mice brains - (reply: 1)
      
1877. cells can't attach in the flasks - (reply: 4)
      
1878. Differential expression of 3 adenoviruses in different cell lines. Why? - (reply: 3)
      
1879. Cell fractionation and isolation of nuclei - (reply: 1)
      
1880. Methanol toxicity in cell culture - (reply: 15)
      
1881. Freezing cells after fix & perm and also a BrdU question - (reply: 4)
      
1882. Freezing cells, quick question - (reply: 1)
      
1883. Best place to buy cells - (reply: 2)
      
1884. help on generation of stable cell lines with pBabe vector - pBabe-puro, phoenix Ampho cells (reply: 7)
      
1885. DMSO usage in cell culture - (reply: 9)
      
1886. stopping brief cell treatments - (reply: 2)
      
1887. Phenol red in media - What metabolic products of cells are acidic? (reply: 2)
      
1888. Cell culture container - (reply: 2)
      
1889. cell lysis method - (reply: 1)
      
1890. Dendritic cell immunology - activated naive cells? (reply: 9)
      
1891. cell contamination???? - (reply: 1)
      
1892. counting number of cells - (reply: 6)
      
1893. Stacking of RAW 264.7 cells - (reply: 1)
      
1894. retroviral generation of stable cell line - cells just don't seem to be growing (reply: 3)
      
1895. Lentiviral transduction of B cells - (reply: 7)
      
1896. Lentivirus transduction of spleen cells - (reply: 2)
      
1897. trouble with establishing a stable cell line - (reply: 3)
      
1898. Cell culture conditions and antigen expression - (reply: 1)
      
1899. Phoenix packaging cell and diphtheria toxin - (reply: 3)
      
1900. Gene expression in cell line - (reply: 1)
      
1901. isoflurane and FACS analysis of peripheral blood? - my cells look terrible everytime I use isoflurane to anaesthetize (reply: 5)
      
1902. Growing Up Cells - (reply: 3)
      
1903. protein extraction after cell lysing - (reply: 2)
      
1904. cell line for neurodevelopment - (reply: 2)
      
1905. Difficulty in doing hemocytometer cell count - (reply: 4)
      
1906. No RNA from cell pellet using Qiagen MiniKit!!??!! - (reply: 5)
      
1907. Method for lysing cells for fractionation - (reply: 4)
      
1908. Neuronal cell culture - why use neonatal brains? - (reply: 1)
      
1909. Detect gene in stable cell line, - (reply: 4)
      
1910. staining cells - (reply: 3)
      
1911. pIRESneo for mammalian cells transfection - (reply: 4)
      
1912. Protocol for staining live cells - Staining live cells for Flow (reply: 2)
      
1913. H9C2 - cell line - Need clarification (reply: 1)
      
1914. Cell plating calculation !! - Help needed with Cell plating calculation !! (reply: 2)
      
1915. Cell lines to study epigenetics - (reply: 2)
      
1916. How to transfect two plasmids in a cell - (reply: 9)
      
1917. Cells dying before i can get them to in vitro assay - (reply: 1)
      
1918. Strange bands in western of RPE-1 cells - (reply: 1)
      
1919. Semisolid Medium For HL 60 cells - (reply: 4)
      
1920. Cell culture contamination (do not know what it was) - (reply: 1)
      
1921. questions about cell lysis - (reply: 7)
      
1922. Gateway Cloning Problem - Lawn of E. Coli Cells - (reply: 4)
      
1923. How to homogenize cell without disruption of nuclei? - (reply: 2)
      
1924. Harvesting cells for flow cytometry - (reply: 10)
      
1925. culture C6/36 insect cell lines - (reply: 1)
      
1926. seeding cell densities - seeding cells at same density (reply: 1)
      
1927. cell culture best practices - virus immortalized and non-immortalized cells (reply: 1)
      
1928. Culturing QT6 cell line - Ideal culture conditions (reply: 2)
      
1929. Calculating MOI for luciferase-transducted cells. - Would the sensitivity of a fluorescent microscope be enough to see the (reply: 3)
      
1930. Picking epithelial, endothelial and neural cell lines - Please suggest best models (reply: 2)
      
1931. How to fix suspencion cells for DAPI detecting under the microscope? - (reply: 6)
      
1932. mycoplasma growth - (reply: 3)
      
1933. primary lung epithelial cells - (reply: 1)
      
1934. Generating double stable cell line - (reply: 3)
      
1935. HACAT cells - very low cell viability after thawing cells (reply: 18)
      
1936. cell line for luciferase assay - How to choose the best cell line? (reply: 2)
      
1937. My cells suddenly die !!! - Please help me ! why is this ? (reply: 11)
      
1938. database for mutations in cancer cell lines? - (reply: 2)
      
1939. Sonication Problems, Branson 450, LNCaP cells - (reply: 4)
      
1940. ? Definition of Passage for suspension cells - (reply: 1)
      
1941. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
      
1942. different staining intensity - fixed permeabilized cells and non-fixed cells (reply: 2)
      
1943. Chip problem: antibody or cell number?? - (reply: 6)
      
1944. stable cell line - (reply: 1)
      
1945. microbial cell viability - any recommendations - assays (reply: 1)
      
1946. SF9 cells not adhering - (reply: 10)
      
1947. HeLa cells - Growth of thawed HeLa cells (reply: 7)
      
1948. Dendritic Cells and Flow Cytometry - (reply: 11)
      
1949. direct cell lysate - (reply: 8)
      
1950. How long can I wait after seeing HeLa cells till transfection - will it affect my Efficiency (reply: 6)
      
1951. Culturing/Mounting of polarised cells for confocal microscopy - (reply: 1)
      
1952. Intestinal cell culture: Protocol required - (reply: 1)
      
1953. Trypan Blue and Hoechst staining.. - am I doing it right?? please help.... (reply: 1)
      
1954. Carbon di oxide free cell culture - (reply: 1)
      
1955. surface staining of THP-1 cell - (reply: 2)
      
1956. How to fix GFP expressing cell to preserve GFP signal in immunofluorescent stain - (reply: 3)
      
1957. Cell Wall Lysis For Dummies - (reply: 4)
      
1958. Tissue & Cell Fixation solutions - (reply: 3)
      
1959. MSP: No PCR product for one cell line but yes for the other cell lines - (reply: 3)
      
1960. Treating cells with chemicals and how to dilute into medium - (reply: 5)
      
1961. T lymphocyte ChIPs are not working... - while other cells are! *grumble grumble* (reply: 6)
      
1962. Using Real Time PCR for Cell Viability - Any tips? (reply: 6)
      
1963. Has anyone used COS-7 cells for retina? - (reply: 1)
      
1964. How to perform a growth curve? - i need to compare 3 cell lines for growth (reply: 2)
      
1965. siRNA concentration in divinding-growing cells - (reply: 2)
      
1966. siRNA transfection of RS4-11 and MV-4-11 Cells - (reply: 1)
      
1967. cell culture guideline from abcam - (reply: 5)
      
1968. Whole cell PCR - DNA purification without using proteinase K? (reply: 8)
      
1969. problems with detecting protein expression - protein expression in cell (reply: 4)
      
1970. freeze PBMC and cell sorting? - (reply: 1)
      
1971. Control vehicle treatment in cells - (reply: 2)
      
1972. Cancer cell culture exploded onto my face - (reply: 8)
      
1973. FACS analysis of GFP expressing cells - Fix or not - (reply: 10)
      
1974. HELP URGENT! FLUID MOSAIC / CELL MEMBRANE - (reply: 2)
      
1975. Sticky Insect cell lysate blocked Ni_NTA column - (reply: 2)
      
1976. Heterogeneous methylation in a same cell line - (reply: 1)
      
1977. siRNA cell function examples - (reply: 1)
      
1978. Good comprehensive resource for maintaining immortal cell lines? - (reply: 1)
      
1979. DH5a comp cells - (reply: 5)
      
1980. Trypsinized cells after fixation - (reply: 3)
      
1981. Detaching Hela cells - (reply: 3)
      
1982. help! Truncated protein cannot expressed in mammalian cells? - (reply: 6)
      
1983. cell cycle softawe - (reply: 3)
      
1984. How to maintain cells at a specific apoptosis stage - (reply: 12)
      
1985. Which cell line produce cytokine? - (reply: 13)
      
1986. Cell Viability - (reply: 1)
      
1987. Shipping of cryopreseved cell - (reply: 15)
      
1988. To what extent competent cells be thawed before transformation? - (reply: 4)
      
1989. Lipofection transfection of HEK293 cells unsuccessful - (reply: 10)
      
1990. Storing HEK293 cells at -80C - (reply: 5)
      
1991. How to control cloned cDNA expression level in cells? - (reply: 8)
      
1992. Asses mycoplasma contamination - (reply: 6)
      
1993. RNA isolation following apoptotic cell labeling - (reply: 3)
      
1994. Why are my E. coli cells not pellet-able? - when expressing a chimeric recombinant protein (reply: 6)
      
1995. Is there an optimal incubation time for cell lysis? - (reply: 7)
      
1996. The way to count adherent cells in 96 well - (reply: 3)
      
1997. lysing cells in LSB - (reply: 2)
      
1998. Using NIH3T3 cells of look at MAPK activation by Ras - (reply: 1)
      
1999. Staining for neural crest derived cells?? - (reply: 2)
      
2000. Dendritic_ cells - (reply: 3)
      
2001. problem to stain just one cell line in coculture - (reply: 4)
      
2002. Inactivation of trypsin - (reply: 4)
      
2003. Culturing stromal cells - (reply: 2)
      
2004. A549 cells - (reply: 4)
      
2005. Cunterstaining Trypan blue - (reply: 6)
      
2006. Chikungunya Virus Culture - Chikungunya Virus culture in mammalian cells form serum sample of huma (reply: 2)
      
2007. Protein extraction from cell culture media - (reply: 9)
      
2008. Bovine cell line for transfection... - Transfection friendly cell line for localization studies... (reply: 1)
      
2009. Has anyone had experience with GM2163 (dam-) cells? - the enzyme BclI is blocked by dam (reply: 1)
      
2010. yeast gene deletion - Could I delete an essential gene from haploid cells? (reply: 8)
      
2011. can we prepare Copmetent cell by calicum cloride with 0.09 OD - Cp cells with 0.09 OD (reply: 3)
      
2012. electroporation of HL60 cells - (reply: 1)
      
2013. Intestinal epithelial cell culture - (reply: 4)
      
2014. SF9 cell transfection using lipofectamine?? - and is CMV active in this cells? (reply: 3)
      
2015. Easiest cell lysis protocol - no interest in preserving proteines (reply: 4)
      
2016. passage number for cell line - (reply: 7)
      
2017. MOCK INFECTED CELLS - (reply: 4)
      
2018. cuboidal cells - (reply: 1)
      
2019. Cell count using a hemocytometer - (reply: 4)
      
2020. Hepatocyte culture - (reply: 4)
      
2021. Buffers that are good for cell culture. - need buffering capacity around pH 5-7 (reply: 3)
      
2022. what is mock cell line? - (reply: 4)
      
2023. 293Flp cell transfection - Passaging cells 4-6 hours after lipofectamine trasnsfection (reply: 3)
      
2024. How to seed the cells evenly in 6 wells plate - (reply: 13)
      
2025. knockout mouse with increase cell size - (reply: 3)
      
2026. Does UV cause DNA fragmentation? - how long do I need to put cells under UV to see fragmentation? (reply: 12)
      
2027. Paper on energy requirement of cells - (reply: 2)
      
2028. galactose induction of yeast cells - (reply: 2)
      
2029. making competent E coli DH5a cells - (reply: 6)
      
2030. How could you explain these results? - cancer cells are weird (reply: 1)
      
2031. strange ES cells contamination - please help!!! (reply: 1)
      
2032. Hek 293 cell line with tetra/doxycycline induction - how to lower expression level? (reply: 2)
      
2033. How to culture PC3 cell line - (reply: 3)
      

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