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Blunt Related Discussions
  1. Blunt-end ligation: what if the vector and insert are both phosphorylated? - (reply: 4)
  2. Polymerase mixture for blunt ended fragments - (reply: 2)
  3. Difficult cloning/ligation/transformation blunt and sticky - (reply: 1)
  4. Sticky and blunt end ligation in single reaction? - (reply: 2)
  5. Blunt end cloning - (reply: 12)
  6. filling the digested vector and insert for blunt end cloning - (reply: 2)
  7. A modified method for blunt end DNA cloning!!!!!!! - (reply: 2)
  8. Sticky and blunt ligation issue - (reply: 6)
  9. Blunt end is ligating to sticky end? - (reply: 2)
  10. Sticky to Blunt end (Hypothetical) - (reply: 2)
  11. Newbie to smaI blunt end cloning! - (reply: 1)
  12. Blunt cloning - No insert, despite lots of colonies - (reply: 3)
  13. ligating blunt ends of a linearized plasmid, is kinase necessary? - (reply: 1)
  14. Cloning problem, sticky - blunt ends ligation fail . - (reply: 6)
  15. help ,blunt end ligation - (reply: 4)
  16. How to clone insert with one sticky and one blunt end into vector? - (reply: 1)
  17. should I do blunt end my pcr product before ligation - (reply: 2)
  18. I can´t obtain the amount of DNA required for ligation of blunt ends - (reply: 2)
  19. Problems with blunt end ligation - (reply: 3)
  20. Blunt end ligation insert:vector ratio - (reply: 1)
  21. Blunt end PCR product ligation - (reply: 1)
  22. Problem with Blunt end ligation - (reply: 4)
  23. Blunt ending (T4) + BAP + Ligation in the same buffer?? - (reply: 2)
  24. Blunt end cloning of 4Kb product - (reply: 3)
  25. BLUNT CLONING With EcoNI - (reply: 1)
  26. blunt and sticky end clonning temperature - (reply: 2)
  27. Is it possible to blunt only either the 5' or 3' end? - (reply: 1)
  28. SmaI blunt end ligation problem... - (reply: 3)
  29. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
  30. Blunt end ligation problems - Could the ligation of sticky adaptors help? (reply: 2)
  31. Unsuccessful cloning with one sticky end & one blunt end - Low transformation efficiency & colonies do not contain insert (reply: 8)
  32. Blunt end filling - Blunt end filling (reply: 1)
  33. No luck with blunt and sticky ligations, need hlep - Prepare for a thorough read (reply: 10)
  34. blunt ligation - (reply: 4)
  35. HpaI blunt end cloning - (reply: 1)
  36. Is it possible to ligate blunt and sticky ends at the same time? - newbie mistake... Please help!! (reply: 11)
  37. Ligation of Blunt PCR Product - (reply: 1)
  38. blunt end ligation problem - Self ligation and truncate (reply: 1)
  39. Blunt end ligation - Cant get ligation to work (reply: 4)
  40. blunt ligation - (reply: 1)
  41. blunt end ligation - how to solve the blunt end ligation?? (reply: 5)
  42. Removal of 5´ overhangs to form blunt ends - (reply: 1)
  43. blunt end cloning - (reply: 4)
  44. question about blunt ligating Taq-amplified PCR product - (reply: 2)
  45. blunt-end / 3´A overhang cloning - (reply: 1)
  46. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
  47. Transcription of double blunt end Clone - (reply: 2)
  48. Blunt-end cloning problem - (reply: 6)
  49. blunt end ligation problem - (reply: 2)
  50. blunt ligation - (reply: 3)
  51. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)