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Band Related Discussions
  1. Why are my genotyping PCR bands blurry? - (reply: 4)
  2. What casues dark zone in middle of DNA band on agarose gel? - (reply: 7)
  3. Light genotyping PCR bands - (reply: 4)
  4. double bands on p-p70s6k WB - (reply: 3)
  5. SDS PAGE bands - (reply: 2)
  6. Lower band detected after alanine mutation - (reply: 3)
  7. Western-No bands for sample protein but loading control works - (reply: 5)
  8. bands are not discrete in PCR, why? - (reply: 1)
  9. Weak band in negative control in PCR - (reply: 5)
  10. Why do I get two bands on the gel of a digested PCR product after purification w - (reply: 1)
  11. Faint band and primer dimer in PCR - (reply: 1)
  12. Problem with RNA bands on RNA bleach gel - (reply: 1)
  13. Anti-Penta His antibody detecting extra bands - (reply: 2)
  14. Unexpected band in BL21 (DE3) plasmid isolation - (reply: 1)
  15. High molecular weight band from my PCR - (reply: 3)
  16. GST fusion protein - two dominant bands - (reply: 7)
  17. bands becoming wavy with colour of the dye changing to yellow - (reply: 1)
  18. 2 bands very close in the expected place - (reply: 2)
  19. SDS-PAGE High MW bands dissapear! - (reply: 7)
  20. PCR bands on gel - (reply: 5)
  21. Electrophoresis problem: Frowning bands and smeary ladder - (reply: 1)
  22. ECM1 on a western blot, why is one band much lower than the rest? - (reply: 1)
  23. black blot images on AI600 imager, .tif shows faint bands - (reply: 1)
  24. No band detected in WB - (reply: 4)
  25. Western Blot diffuse bands - (reply: 3)
  26. Some bands transferred, some not..? Why??? - (reply: 1)
  27. Extraction of bands from SDS-PAGE - (reply: 2)
  28. Dumbbell-shaped bands on the WB - (reply: 3)
  29. Deformed bands in gel - (reply: 8)
  30. no bands after pcr!!!! - (reply: 4)
  31. Help in western blotting GAPDH bands are not even - (reply: 1)
  32. Thrombin cleaved GST-fusion protein- multiple bands - (reply: 2)
  33. detection of a protein band of protein complex - (reply: 3)
  34. How to determine gene knock-out from Agarose gel bands - (reply: 1)
  35. Two bands in WB but one band in IP - (reply: 1)
  36. Two bands in WB but one band in IP - (reply: 2)
  37. White bands in western blotting - (reply: 4)
  38. Colour of PCR gel band best for ImageJ analysis - (reply: 2)
  39. colony pcr, two strong bands - (reply: 4)
  40. Western blot problem....laddering pattern of white bands on Nitrocellulose membr - (reply: 5)
  41. Problem with Western Blotting - BCA assay shows protein but no protein bands whe - (reply: 3)
  42. PCR DNA bands too large on agarose gel - (reply: 1)
  43. Some bands of similar intensity aren't seen in short exposure but are seen i - (reply: 1)
  44. All bands, including ladder, appear as double bands in EtBr stained gel - (reply: 6)
  45. RNA gel problem (melted bands) - (reply: 2)
  46. The slightly bigger band dillema. - (reply: 2)
  47. Non especific band in PCR... - (reply: 2)
  48. Smeary bands in gel electrophoresis - (reply: 3)
  49. band obove the ladder - (reply: 3)
  50. Problems with SgrAI digest, ligation, killer cut and weird bands on gel - (reply: 4)
  51. Contamination in qRT-PCR RT-minus - band similar to cDNA, not sure what it is? - (reply: 2)
  52. unexpected protein bands - (reply: 2)
  53. Smiling bands in Western blot - revisiting a widely asked question - (reply: 3)
  54. Band did not migrate on gel electrophoresis (SDS) - (reply: 3)
  55. RNA Gel 3 Sharp bands? - (reply: 1)
  56. PCR product and Restriction products bands are not of the expected size - (reply: 4)
  57. Gel Bands - (reply: 1)
  58. No bands can be seen in my co-IP - (reply: 3)
  59. large bands in all pcr reactions including negative control - (reply: 1)
  60. reason for curved bands? - (reply: 2)
  61. Protein bands visible on unstained SDS-PAGE - (reply: 1)
  62. Digestion bands appear faint - (reply: 3)
  63. several bands in non-reducing SDS-page gel - protein without cysteines - (reply: 2)
  64. double band with anti-Flag but not with anti-HA antibody - (reply: 1)
  65. Why I’m not getting any band after column purification? - (reply: 1)
  66. Strange band pattern in Western Blot - (reply: 1)
  67. Western Blot: protein band did not appeared at the correct location - (reply: 3)
  68. i am trying to get rid of unexpected protein band by centricon - (reply: 3)
  69. Mysterious disappearing bands after blotting - (reply: 5)
  70. Western blot with smeared bands - (reply: 3)
  71. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
  72. Thick 100-bp band disappeared under UV - (reply: 3)
  73. Enzyme digestion resulted in extra bands from plasmid miniprep product - (reply: 10)
  74. Electrophoresis after PCR : too many bands - (reply: 6)
  75. Nanog double band in WB - (reply: 1)
  76. My beta actin bands are changing - (reply: 6)
  77. Why does my gel have streaks instead of bands? - (reply: 2)
  78. Band on Western Blot are smeared - (reply: 1)
  79. PCR with no bands showing in 1.1 % gel electrophoresis - (reply: 4)
  80. Need help to identify bands in AGE result - (reply: 3)
  81. EMSA Shifted band in Free probe only lane - (reply: 1)
  82. Please help uneven bands in western blot - (reply: 9)
  83. Western Blot Problems...too many unspecific bands - (reply: 4)
  84. I never see the 16kDa band from the seeblue marker? - (reply: 3)
  85. Appearance of a higher molecular weight band after plasmid isolation? (NOT chros - (reply: 2)
  86. Bands near edges running faster - (reply: 4)
  87. faint dna band after pcr purification - (reply: 1)
  88. Need help with dCAPS pcr, seeing huge bands on gel - (reply: 1)
  89. Smeary bands on agarose gel (also the ladder) - (reply: 2)
  90. No Band With Large DNA (BAC) - (reply: 1)
  91. relationship between protein size, lysate concentration, and band intensity - (reply: 3)
  92. i couldn't get the band of target gene after ligation - (reply: 9)
  93. IP: specific bands in control lanes - (reply: 1)
  94. Multiple Bands after gel extraction - (reply: 4)
  95. DNA extraction problem - strange bands - (reply: 4)
  96. Help!! Double band in genomic dna - (reply: 1)
  97. No bands despite different primers and conditions and TAqs - (reply: 8)
  98. unwanted band - (reply: 5)
  99. Dotted bands on my DNA agarose electrophoresis gel. - (reply: 2)
  100. strong 100kda bands for alpha tubulin + the usual 55 kda bands? URGENT - (reply: 3)
  101. How to get rid of bands in PCR negative control - (reply: 10)
  102. T7 and M13 primers two band amplification - (reply: 3)
  103. Disappeared bands after the addition of Sigma P2714 - (reply: 1)
  104. Diffuse rRNA bands on RNA denaturing (formaldehyde) gel - (reply: 5)
  105. Prey protein band bleeds, IgG band normal? - (reply: 3)
  106. negative control well 300 bp band - (reply: 3)
  107. How to confirm the band of interest in Westerns blot - (reply: 6)
  108. why i get double band for recombinant protein - (reply: 9)
  109. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
  110. Western Blot-No Sample Band, but Positive Control band visible - (reply: 10)
  111. Distorted bands from human colon samples - (reply: 2)
  112. "Wavy" bands in SDS-PAGE gel - (reply: 5)
  113. I get band double the size what does it mean - (reply: 3)
  114. Smeared Bands after Ponceau Staining? - (reply: 3)
  115. RFLP problem - missing band - (reply: 2)
  116. patchy/holey bands after transfer, invasive bubbles? - (reply: 7)
  117. fused bands of beta-actin in western blot - (reply: 2)
  118. how to get specific bands in co-ip - (reply: 5)
  119. faint band after plasmid extraction - (reply: 7)
  120. Undefined bands in Gel - (reply: 9)
  121. are they non-specific band? - (reply: 4)
  122. Trouble with purified recombinant protein bands - (reply: 3)
  123. 3 extra bands show up on my western after an IP - (reply: 2)
  124. Agarose gel bands gone (for gel extraction) - (reply: 2)
  125. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
  126. 3' RACE creates too big band doesn't leave well - (reply: 1)
  127. RT-PCR product- no band - (reply: 4)
  128. Wierd Bands after PCR....Confused - (reply: 9)
  129. Quantify DNA in gel electrophoresis bands - (reply: 7)
  130. Western Blot: only non-specific bands and not protein of interest - (reply: 3)
  131. Restriction digest band shift? - (reply: 1)
  132. No bands/weird bands on western blot - (reply: 3)
  133. Straight bands on DNA gel - (reply: 3)
  134. Western blot- bands weak - (reply: 11)
  135. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
  136. 10,000 bp size band showing up in gel of plasmid extraction - (reply: 1)
  137. Curved bands in Western Blot - (reply: 2)
  138. Some bands not coming up, others uneven - (reply: 9)
  139. No bands in PCR after DNase treatment - (reply: 4)
  140. Multiple Non-Specific Bands in Western Blot Detected... - (reply: 1)
  141. B- actin band looks like dumbbells! - (reply: 2)
  142. Which antibody is good for HA-tag with low background bands? - (reply: 1)
  143. inespecific bands in nuclear fraction? - (reply: 1)
  144. PCR band slightly BELOW expected length ?! - (reply: 14)
  145. unexpected band in PCR with plasmid - (reply: 4)
  146. cloning: band at different site than desired after RE digestion - (reply: 11)
  147. bands position in agarose gel - (reply: 4)
  148. Multiple Bands in qPCR - (reply: 4)
  149. SDS PAGE - Band artifacts when protein buffer is pH 5.0 and heated - (reply: 2)
  150. White bands on Cy3 gels - (reply: 1)
  151. Western Blot, Bax, Many bands - (reply: 3)
  152. Odd Bands on Gel - (reply: 3)
  153. qPCR: band in gel, but super high Cq? - (reply: 10)
  154. SDS Page and WB: Wrong size bands - (reply: 2)
  155. myc-tag antibody makes multiple bands, even in mock & parental cells - (reply: 1)
  156. Please help with odd bands and smears on Western blot - (reply: 4)
  157. Shift in the position of band onto the western blot - (reply: 3)
  158. Ghost and inverse bands and high background. Help! - (reply: 3)
  159. No resolving of protein/bands below 50kDa :-s - (reply: 5)
  160. "Mickey Mouse" bands - (reply: 5)
  161. aditional band on WB - (reply: 1)
  162. Bands dark on sides and light in middle - (reply: 7)
  163. nonspecific band at 50bp in PCR - (reply: 9)
  164. Protein bands not migrating - (reply: 3)
  165. Single Plasmid Gel Band - (reply: 1)
  166. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
  167. Detecting doublet bands in Western for eGFP control - (reply: 1)
  168. Help with PCR and Gel Electrophoresis. Not getting any bands - (reply: 8)
  169. Uneven bands denaturing agarose - (reply: 2)
  170. two bands on WB-alternative use of start codons? - (reply: 2)
  171. PCR band moves lower than its size on agarose gel - (reply: 3)
  172. No WB bands for H2AX at all...! - (reply: 10)
  173. Overexpressed protein shows a band higher than expected?! - (reply: 2)
  174. Help! No bands after BSP! - (reply: 3)
  175. Multiple bands on blot with one antibody - (reply: 1)
  176. Multiple protein bands after expression - (reply: 6)
  177. Bands in negative control PCR - (reply: 2)
  178. No bands at all after double digestion - (reply: 1)
  179. Non-specific bands and fuzzy bands - (reply: 1)
  180. Weird extra bands in EMSA - (reply: 1)
  181. Help for western blot wet transfer blurry bands - (reply: 2)
  182. Blurry bands ... - (reply: 12)
  183. Strange Bands - (reply: 2)
  184. smearing below band of interest - (reply: 5)
  185. After PCR, One Band on 1.2% Agarose but 2 bands on 8M Urea, 8% PAGE - (reply: 1)
  186. No or very weak band using 16s primers - (reply: 1)
  187. band shift after IP - (reply: 1)
  188. Western blot narrow bands - (reply: 1)
  189. Bizarre changes in plasmid band size - (reply: 3)
  190. Western blot band anaylisis - (reply: 3)
  191. RNA degradation problem, only one band on the gel - (reply: 3)
  192. Smaller band appear under Main product with every primers sets from PCR - (reply: 3)
  193. 16s double bands obtained-why?? - (reply: 2)
  194. Why do supposedly sharp bands sometimes appear unclear in agarose gel? - (reply: 1)
  195. got band in control positive but not in sample... - (reply: 2)
  196. Band is at the wrong size, but same for all antibodies - (reply: 6)
  197. Puzzeling western blot results, missing band - (reply: 3)
  198. No bands appearing in DNA gel electrophoresis - (reply: 1)
  199. Multiple bands in eluted PCR product - (reply: 1)
  200. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 5)
  201. BAC miniprep-high nanodrop value but NO BAND - (reply: 2)
  202. multiple bands for genomic DNA extraction - (reply: 3)
  203. The same strong bands with different primary antibody - (reply: 1)
  204. many bands Western Blot - (reply: 2)
  205. unespecific band PCR from mouse genomic DNA - (reply: 9)
  206. Help with unknown band in restriction digest - (reply: 1)
  207. Preference for larger of 2 bands? - (reply: 2)
  208. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
  209. RNase Band in old plasmid stored at -40 - (reply: 2)
  210. getting rid of non specific bands in IP experiments - (reply: 7)
  211. strange bands shape agarose gel - (reply: 2)
  212. MMP-9 westernblot extra bands at 25kda and 60kda - (reply: 6)
  213. Getting too Many Bands on my Western Blot - (reply: 9)
  214. Extra bands after protein expression/purification - (reply: 4)
  215. sequencing result from excised DGGE bands - (reply: 3)
  216. Blue colonies with insert, white colonies with multiple weak bands - (reply: 5)
  217. Problem with merged bands within western blot caused by amount of protein loaded - (reply: 7)
  218. Immunoprecipitation---Why would a 250kDa band be present for the purification of - (reply: 8)
  219. Birdlike bands/lanes of SDS-Page and WB - (reply: 2)
  220. Analyzing Western Bands by Densiometry - (reply: 2)
  221. Problem: Cloning DNA shuffled band - (reply: 2)
  222. Problems with a PCR-No bands and I am starting to question my sanity - (reply: 4)
  223. my protein bands and protein marker becomes abnormally clearer sometimes - (reply: 3)
  224. Problem with PCR - large extra bands - (reply: 3)
  225. Same non specific band for different target proteins - (reply: 1)
  226. a band that suppose to be 90KD shows up at 70KD!! - (reply: 1)
  227. Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
  228. No band in PCR - (reply: 5)
  229. Identically loaded western bands loose intensity from left to right - (reply: 3)
  230. smear bands in western blot - (reply: 12)
  231. bands fuse together - (reply: 1)
  232. Measuring of cDNA bands density - (reply: 3)
  233. No band after extracting DNA from bacillus subtilis - (reply: 1)
  234. Can proteins with sizes smaller than 60 kda be seen as bands in a 6% gel western - (reply: 3)
  235. Bands from the first detection appear at the second detection but with a differe - (reply: 1)
  236. Re-using membranes with ghost bands - (reply: 2)
  237. huge dot appears at the centre of every of my bands - (reply: 2)
  238. Bright bands of PCR products stick in gel wells - (reply: 1)
  239. Separating close bands in Western Blot - (reply: 3)
  240. Didint get any band during gel electrophoresis even though DNA was there - (reply: 5)
  241. Bands in my PCR Controls - (reply: 3)
  242. Strange band in colony PCR - (reply: 2)
  243. overexpression FLAG-tagged protein shows extra bands in WB - (reply: 2)
  244. Bands seen with qPCR missing in regular PCR - (reply: 5)
  245. non specific bands - (reply: 7)
  246. why cant get the band? - (reply: 4)
  247. Western Blot no target bands, show Non-specific bands - (reply: 5)
  248. Bands disappear after longer exposure - (reply: 4)
  249. clear bands on agarose gel but problems in melting curve after qPCR - (reply: 2)
  250. How to remove the background and unspecific bands - (reply: 12)
  251. Same Non-specific bands show up for different Abs!! - (reply: 7)
  252. what caused bubble band of my WB? - (reply: 4)
  253. Strange bands and large smear in western blot - (reply: 4)
  254. GAPDH - Non-specific bands with GAPDH monoclonal antibody (reply: 1)
  255. HELP!- strange band appearing on immunoblot - strange band detected during blotting with anti-CTB antibodies (reply: 3)
  256. PCR: Band in one lane, streaking in the other..HELP! - (reply: 3)
  257. Band Quantitation - Bio-Rad's Quantity One (reply: 1)
  258. strange and branch like bands on SDS-PAGE - troubleshooting (reply: 7)
  259. how to get rid of non specific bands in PCR - (reply: 1)
  260. Help -Water Blank keeps coming up with mutiple bands - (reply: 8)
  261. Heavy Post-translational modifications - Multiple bands on gel (reply: 2)
  262. SDS PAGE smearing of bands - (reply: 2)
  263. Fuzzy bands in western blot - (reply: 2)
  264. Large Non-specfic bands in PCR - (reply: 3)
  265. HELP!! I ruin my Native-PAGE (newbie in PAGE) - My bands seem faint smear (reply: 3)
  266. 2 bands in blue colony PCR - (reply: 5)
  267. no bands after pcr - even after we have change most of the reagents (reply: 2)
  268. Identify ponceau visible bands - (reply: 1)
  269. Please help! no PCR bands+Description of my PCR protocol provided :( - (reply: 8)
  270. Many unspecific bands after using anti-His on Western blot - (reply: 4)
  271. no band observed for bacterial genomic DNA during agarose gel electrophoresis - (reply: 9)
  272. KO mice show band for knocked-out protein - (reply: 1)
  273. western blot bands wider at the middle than the top - (reply: 2)
  274. PCR Gel nonspecific band - (reply: 13)
  275. can't get bands in Western Blot - (reply: 5)
  276. difference in band sizes ! - why ??? (reply: 2)
  277. Funny DNA bands on Agarose gels - (reply: 6)
  278. Actin Bands smiling on western blot - (reply: 3)
  279. PCR: always got band in the negative control - (reply: 8)
  280. nonspecific bands on film - (reply: 3)
  281. 'lace-like texture' on my northern blot bands - (reply: 8)
  282. Problem Viewing bands in Agarose Gel - (reply: 6)
  283. Troubleshoot PCR, Product band missing, internal control is present - (reply: 1)
  284. software for quantifing PCR product band - (reply: 1)
  285. Multiple bands from purified PCR product - (reply: 3)
  286. Quantification of proteins in Western Bands - (reply: 2)
  287. No bands on agarose gel after restriction digestion. - (reply: 10)
  288. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
  289. Extra band on gel after miniprep - (reply: 6)
  290. 3 distinct bands of gDNA on gel - (reply: 2)
  291. Fat bands on 15% SDS-PAGE gel - (reply: 10)
  292. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
  293. Total RNA only produce 1 band in gel - (reply: 2)
  294. Gel gets squiggly bands for low molecular weight proteins - (This is driving me insane) (reply: 7)
  295. how many minimum and maximum number of bands in SSCP marker? - (reply: 2)
  296. weird bands after minipreps - molecular cloning (reply: 2)
  297. Band at same position using different primary antibodies - (reply: 3)
  298. purified antigen and ELISA - non-specific bands in purified antigen for ELISA use (reply: 2)
  299. PCR, using gel extracted band as a template - (reply: 4)
  300. Glyoxylated RNA and SYBR Safe - Running glyoxylated RNA on a SYBR safe agarose gel - NO BANDS (reply: 1)
  301. PCR bands in NTC but NOT in negative samples - (reply: 2)
  302. Weak Zymography Signals - Mitigating a weak signal for better bands (reply: 1)
  303. Unusual band in transformants - (reply: 2)
  304. mirna overexpression/ library versus single construct/ unspecific pcr - unspecific band when pcr on gDNA from library but not on gDNA fron sin (reply: 2)
  305. Better images of agarose gel - My bands are not clear when i take a image for processing (reply: 3)
  306. no band, extremely clean blot - (reply: 1)
  307. Gel electrophoresis - Sudden untidy bands despite changing buffer, low yields from gel purif (reply: 4)
  308. Loss of bands at higher Tm in presence of DMSO... - Attempt to optimise DMSO methylation-spec PCR (reply: 4)
  309. IgG - unspecific band (reply: 3)
  310. SDS-PAGE resolution - Problem in resolving high molecular weight bands on SDS-PAGE (reply: 3)
  311. "Unzipped" PCR band - (reply: 3)
  312. Gel Extracting a 15kb Band? - Quick and Easy method (reply: 3)
  313. uncut vector contamination in gel purified band - (reply: 2)
  314. I see bands with western blot only the third day! - (reply: 2)
  315. incorrect/smaller band size for real time PCR - (reply: 1)
  316. Agarose gel bands smear/ drag problem - (reply: 4)
  317. Northern - Do not get Bands in Northern Blot (reply: 2)
  318. two bands detected in western - (reply: 1)
  319. Protein SDS-PAGE thick bands / smear - (reply: 4)
  320. No protein lane have bands on Coomassie and western blot?! - (reply: 4)
  321. New smeared bands for protein that previously ran fine - Smeared protein bands now but not the previous 50 times I've run same (reply: 1)
  322. need help plz, band sizes estimation - (reply: 2)
  323. What causes this offset band on RIPA gel? - (reply: 4)
  324. nicotinic receptor western blot - a3 nAChR band at 100kDa instead of 55kDa (reply: 2)
  325. band diffusion in sds - (reply: 5)
  326. too much bands in western - (reply: 2)
  327. Fusion PCR, bright smear from well to end(with very weak or no band) - (reply: 1)
  328. Denaturing formaldehyde agarose gel for RNA - Once formaldehyde is added, I see no bands (reply: 8)
  329. Bands are lower than actual protein - (reply: 1)
  330. PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
  331. No band after WB revelation - (reply: 2)
  332. No band after digestion - (reply: 4)
  333. fast Chip protocol..high MW bands and crabby 260/230 ratio.. - (reply: 8)
  334. electrophoresis/ difference between expected size and result/ what's the alt - How to confirm the band means the expected DNA? Sequencing&what else? (reply: 5)
  335. PCR -No band formation - (reply: 1)
  336. PCR - No Band formation - (reply: 3)
  337. No bands in cytosolic extraction, nice bands in nuclear extraction--see pics - (reply: 1)
  338. 2d page - no band (reply: 2)
  339. never get bands by pfu related enzymes - (reply: 2)
  340. DNA bisulphited Amplification - the amplification dont produce any band!! (reply: 3)
  341. Lower MW bands than expected. - (reply: 1)
  342. Multiple bands after bisulfite PCR - (reply: 7)
  343. western bands look funny - (reply: 5)
  344. SDS-PAGE: Protein bands broaden as it migrates down the gel like a pyramid! - (reply: 2)
  345. quantifying "ghost" bands - (reply: 1)
  346. Ponceau Bands, Marker transfer, but no bands? - Ponceau positive, the marker transfers, no bands? (reply: 2)
  347. Extra bands after QIAGEN spin miniprep - (reply: 3)
  348. agarose gel of ligated DNA doesn't show any bands - (reply: 2)
  349. Cold probe doesnt compete away bands - (reply: 2)
  350. Strength of actin signal - How dark should the band be? (reply: 5)
  351. Bands gradually weaken and disappear after putting the gel on the UV transllumin - (reply: 3)
  352. PCR product appear two close band in my gel - (reply: 15)
  353. The Case of a Missing Band: PCR Issue - (reply: 4)
  354. PCR: Smear bands, no amplification, got it all.. - PCR Mutagenesis (reply: 5)
  355. Band appear and disapper in the negative control ?? - (reply: 2)
  356. Bands in PCR negative control - (reply: 3)
  357. Agarose Gel Electrophoresis Problems - DNA bands appear to be weird on the gel (reply: 3)
  358. western exposure one part of band darker - (reply: 6)
  359. non-specific bands - (reply: 5)
  360. Tips for visualizing very faint bands in agarose gels? - (The PCR ain't going to get better, so the gel must!) (reply: 15)
  361. bands of protein seems like it did not move - (reply: 2)
  362. downshifted band - (reply: 4)
  363. no black band but empty stamp of the protein - instead of black bands in xray film, the space is empty with a stamp o (reply: 5)
  364. problem in western blotting, picture attached - spots in xray film but no bands (reply: 4)
  365. 3x flag tagged cloning -> western -> multiple bands but not non-specific - Please help me!! (reply: 1)
  366. Cloning -> expression -> i see multiple bands :( - overexpression of my 3x flag tagged protein shows 4 bands.. (reply: 5)
  367. PCR- trouble shooting - faint band in the negative control (no template) - (reply: 8)
  368. more than one band - (reply: 1)
  369. IGg bands on SDS PAGE - (reply: 2)
  370. PCR band too thick - PCR troubleshooting question (reply: 5)
  371. ugly merged bands in western blot - (reply: 3)
  372. PCR bands on gel electrophoresis - (reply: 5)
  373. Identify the band in western blot - (reply: 5)
  374. Ponceau staining - Appearence of bands (reply: 1)
  375. Multiple bands after amping insert - (reply: 2)
  376. Protein bands on the bottom of the gel - (reply: 5)
  377. Increasing Separation of Bands on SDS PAGE gel - Increasing Separation of Bands on SDS PAGE gel (reply: 2)
  378. Why is there extra band in IMAC SDS-PAGE - (reply: 1)
  379. Bands in a miRNA gel - (reply: 1)
  380. Gel Electrophoresis band problems - (reply: 3)
  381. Problem with protein samples - No bands detected at all on gel (reply: 2)
  382. Unhappy bands - (reply: 3)
  383. High MW PCR band seen...help needed - (reply: 7)
  384. Western Bizarre - Is it the lack of SDS cause the twisted and partially diffused bands？ (reply: 9)
  385. HELP! Genomic DNA, RNA-size bands - (reply: 2)
  386. No protein bands in SDS-PAGE after sample clean-up steps! HELP! - (reply: 8)
  387. "Diffuesed" bands of higher molecular weight - (reply: 6)
  388. Transfection and WB - Transfected cell line shows incorrect bands on WB (reply: 2)
  389. strange band in coomassie blue staining - (reply: 3)
  390. nonspecific band in transfected c-abl cells - nonspecific band in transfected c-abl cells (reply: 1)
  391. Secondary Antibody reacting with Loading Buffer? Is this possible? - Having problems with unidentified bands in western blot (reply: 3)
  392. why the bands migrate lower in the gel EMSA? - as I increase protein concentration (reply: 1)
  393. Different mol wt band compared to bioinformatic prediction algorithms - (reply: 4)
  394. PCR troubleshooting - two band after agrose electophoresis (reply: 4)
  395. Wrong bands on the blot - (reply: 4)
  396. Problems detecting my band! - (reply: 2)
  397. Western bands fading out, pleeeese help - (reply: 1)
  398. western blot w/ anti-FLAG ab - multiple bands show up (reply: 7)
  399. Why do I always get these ugly bands？ - I always end up with some fused and tandemed bands... (reply: 7)
  400. Agarose Gel Electrophoresis - How to prove two bands are same size? - (reply: 3)
  401. Bands visible in membrane but not in the film - ?? (reply: 10)
  402. non desired brigther bands - (reply: 7)
  403. Bands on adjescent lanes!!! - wierd!!! (reply: 11)
  404. Only DNA ladder , No desired band in PCR - (reply: 4)
  405. Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
  406. Lost a Band by changing thermocycler - (reply: 9)
  407. problem: doublets and triplets of bands occur in SDS-PAGE - (reply: 5)
  408. Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)
  409. how to get sharp band for restriction enzyme - how to get sharp band for restriction enzyme (reply: 2)
  410. Confusing bands from PCR - not primer dimer, not product (reply: 30)
  411. Bands appearing out of no where?! - (reply: 5)
  412. EXtraction of DNA bands from acrylamide gel - (reply: 4)
  413. High Molecular weight artifact LA-PCR 2-step - Artifact or real band - higher than expected Mw amplified product (reply: 3)
  414. Another band after plasmid purificaiton - ran the plasmid DNA on a gel (reply: 2)
  415. casting agarose gel troubleshooting - smeared bands (reply: 8)
  416. How to analyze the ingredients of protein bands in native-PAGE gel? - help (reply: 3)
  417. Pulse Field Gel Electrophoresis - smear at lower molecular weight bands (reply: 6)
  418. the bromophenol blue band is skew during agarose electrophoresis - (reply: 1)
  419. overloading GAPDH but nice band of interest - (reply: 2)
  420. Quantiying bands. - Determination of protein contens (reply: 2)
  421. after re-isolation of vector, get single band - (reply: 4)
  422. BSP PCR for help - no bands of tissue amplification (reply: 3)
  423. 2 bands or smear? 2-step RT-PCR - (reply: 1)
  424. Too many bands in my SDS-PAGE - (reply: 5)
  425. Uneven bands in SDS-PAGE - (reply: 5)
  426. weak bands for methylated DNA - (reply: 4)
  427. Two bands for p53 on Western Blot - (reply: 7)
  428. nonspecific bands after co-ip - (reply: 2)
  429. No PCR bands with some templates - (reply: 5)
  430. EMSA - Band doesn't shift with poly dI-dC - (reply: 2)
  431. Blurred low molecular weight bands in SDS-PAGE - Need your help (reply: 1)
  432. Is possible to generate an anomalous electrical field? - I get diffuse bands, because of electrical field???? (reply: 1)
  433. uggly, non-focus and diffuse bands in my Western.. - Can you see my image of my Western to LC3? (reply: 1)
  434. How to solve this western bolt problem? - dumbbell-shape bands (reply: 2)
  435. light bands, dark smear, dark dimers - (reply: 2)
  436. pcr doesnt show any band for DNa walking - (reply: 8)
  437. Immunoprecipitation - Bands in negative control - (reply: 2)
  438. crappy bands after developing - (reply: 3)
  439. Control Restriction Digestion - got one additional band - what does this mean? (reply: 5)
  440. no bands in my PCR - (reply: 9)
  441. All gradient samples give bands! How come? - (reply: 4)
  442. many bands of smaller size than expected - (reply: 3)
  443. wrong bands after T-vector cloning - (reply: 3)
  444. abnormal band in agarose gel - Urgent help required (reply: 3)
  445. Ghost bands in western blot - Western blotting problems (reply: 9)
  446. PCR contamination with human DNA first and now no bands!!! - (reply: 4)
  447. Fragmented DNA doesn't appear as bands on agarose gel - (reply: 5)
  448. non-specific band persistent at 29kDa - western blot non-specific bands (reply: 2)
  449. Non-specific bands on EMSA - (reply: 2)
  450. Always a Band in negative after RT-PCR - RT-PCR and Gapdh (reply: 2)
  451. Unwanted 100bp in RT-PCR - 100bp band appearing all the time after RT-PCR (reply: 7)
  452. PCR ghost bands - (reply: 1)
  453. Co-IP problems with band sizes - Different band sizes in input and pulled down (reply: 1)
  454. strange extra bands on digest of miniprep? - (reply: 5)
  455. GST preotin non specific preotins - Non specific GST bands (reply: 2)
  456. 3'RACE and the polyA tail - why only one band? (reply: 2)
  457. higher MW non specific band - 90-100 kDa band always present, WT or KO (reply: 3)
  458. A single band on WB smaller than expected - (reply: 3)
  459. Low sized bands in plasmid preperations - its not RNA! (reply: 13)
  460. Co-IP problem - Weird IgG band (reply: 1)
  461. Problem with Maxi Prep - Extra unknown bands appear after test digestions (reply: 3)
  462. EMSA supershift - increased band intensity? - (reply: 1)
  463. DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
  464. no band in gel - gel electrophoresis (reply: 1)
  465. SDS PAGE of Bovine Serum albumin - Why more than one band? (reply: 6)
  466. Strange bands in western of RPE-1 cells - (reply: 1)
  467. no 18S band on RNA agarose gel - (reply: 1)
  468. Reamplification after agarose gel extraction - problems with reamplification of a band after purification form gel (reply: 4)
  469. multiple bands in my RNA sample? - (reply: 2)
  470. multiple bands in my RNA sample? - (reply: 2)
  471. Ghost band in SDS PAGE - (reply: 2)
  472. No band in the gel after ligation - (reply: 6)
  473. Unidentified DNA bands - (reply: 1)
  474. silica purification - problem with double bands after silica purification (reply: 1)
  475. PCR bands in NTC Control and Neg Control Lanes - (reply: 4)
  476. normal PCR - two bands is found in one sample (reply: 3)
  477. Digest genomic (eukaryote) DNA before running PCR, is it necessary? - I do not get any band from PCR using genomic DNA and different primers (reply: 6)
  478. After double digestion, one weird band on the very top! - (reply: 4)
  479. After Double Digestion, there is one band on the very top. What is it? - (reply: 2)
  480. Immunoprecipitation (optimization?) problem - anti-ovalbumin lane has 225 kDa band. (reply: 7)
  481. strange double band in electrophoretic pattern - (reply: 2)
  482. protein band size determination - any software available? (reply: 2)