Western blotting Methods, Protocols and Troubleshootings

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Western blotting Related Discussions

eliminating albumin from samples for western blotting ?. - (reply: 1)
double bands on p-p70s6k WB - (reply: 3)
Preventing contamination in TBS/T (WB wash buffer) - (reply: 5)
Can anyone help me with Western blotting problem? - (reply: 6)
western blotting troubleshooting - (reply: 3)
Question on western blot picture - (reply: 2)
how to interpret this WB result - (reply: 7)
SDS-PAGE/2D gel and western blot for multiple proteins for multiple sample - (reply: 1)
coIP followed by a Western Blot - (reply: 4)
WB imaging on Vilber-Lourmat Fusion - (reply: 1)
Low weight protein degradation during western blot - (reply: 1)
Flow Cytometry vs Western Blotting: can't get the same result. Why? - (reply: 1)
phospho-creb western blotting - (reply: 3)
bacterial culture volume for recombinant protein to be detected in Western Blot - (reply: 3)
Non-specific binding of protein in western blot - (reply: 3)
2D PAGE and Western blotting - (reply: 1)
Lane spillover (?) on Western Blot - (reply: 3)
ECM1 on a western blot, why is one band much lower than the rest? - (reply: 1)
No band detected in WB - (reply: 4)
Western Blot diffuse bands - (reply: 3)
Human cell membrane protein Western blot! - (reply: 2)
Bubbles in western blot gel "AFTER" running gel - (reply: 2)
pIKK antibody for western blot (RAW 264.7 cells) - any recommendations? - (reply: 1)
Western Sensitivity: ECL versus DyLight 800 Detection - (reply: 3)
MAb detected on ELISA but no mIgG detected in WB - (reply: 1)
what should be used as controls during western blotting? - (reply: 4)
Dumbbell-shaped bands on the WB - (reply: 3)
Help in western blotting GAPDH bands are not even - (reply: 1)
37kb and 7kb protein in western blot - (reply: 24)
Dot blot/Western background signal problem - (reply: 1)
SDS-PAGE protein analysis with antibodies alone (not WB) - (reply: 1)
What is best to measure protein expression: western blot or ELISA? - (reply: 4)
Two bands in WB but one band in IP - (reply: 1)
Two bands in WB but one band in IP - (reply: 2)
Western blot detects exogenous protein but not endogenous - (reply: 2)
White bands in western blotting - (reply: 4)
How to obtain a control positive for my WB - (reply: 3)
Western blot problem....laddering pattern of white bands on Nitrocellulose membr - (reply: 5)
Problem with Western Blotting - BCA assay shows protein but no protein bands whe - (reply: 3)
Question on loss of signal between samples in western blot - (reply: 4)
Sample prep of cells for FACS analysis and running WB - (reply: 4)
PCR & Western Blot sample preperation - (reply: 2)
PCR & Western Blot sample preperation - (reply: 5)
How to optimise my western semi-dry transfer? - (reply: 3)
Help on native PAGE gel and its western blot result - (reply: 5)
Smiling bands in Western blot - revisiting a widely asked question - (reply: 3)
No protein on western blot but standard transferred - (reply: 2)
Can't detect GAPDH (western) with Alexa488 secondary antibody - (reply: 6)
Western blotting - methods to detect two proteins of a similar size? - (reply: 2)
how to choose a proper tag for western blotting - (reply: 6)
Strange band pattern in Western Blot - (reply: 1)
Western Blot: protein band did not appeared at the correct location - (reply: 3)
RIPA buffer for IP and western - (reply: 1)
Problems of western signal - (reply: 5)
Western Blot Help - (reply: 1)
Western Blot: Unequal Sample Migration - (reply: 4)
Western blot with smeared bands - (reply: 3)
Blocking agent in western blotting of histone proteins - (reply: 1)
Best western loading control for comparing different cell lines - (reply: 2)
western blotting running problem? - (reply: 2)
Keeping apoptotic/detached cells for WB? - (reply: 3)
Advice with WB please - (reply: 3)
Western Blot Wet Transfer Conditions - (reply: 10)
Western Blot and Inmunofluorescence contradictions - (reply: 12)
Nanog double band in WB - (reply: 1)
Help with western blot!! - (reply: 1)
Band on Western Blot are smeared - (reply: 1)
Can Anti-IgG Horseradish peroxidase be used in a whole blood western blot assay? - (reply: 1)
advice in western blotting - (reply: 1)
can antibodies recommended for western blot be used for immunopreipitation - (reply: 2)
Please help uneven bands in western blot - (reply: 9)
Chemiluminescence vs. Fluorescence visualisation of western blot - (reply: 1)
Cell Lysate Western Blot Little or No Signal - (reply: 7)
Need Help with Western blot - (reply: 1)
Western Blot Problems...too many unspecific bands - (reply: 4)
IL 8 - western blotting - (reply: 1)
how to make fast blocking buffer in western blot? - (reply: 1)
Wester blot analysis - (reply: 5)
Protein bandshift in western blot of cell lysate vs immunoprecipitation eluent - (reply: 4)
timecourse experiment for Western and qPCR - (reply: 2)
Western blotting - (reply: 4)
Fixing concentration for western blot from MTT - (reply: 1)
Western Blotting of Amyloid Beta - (reply: 1)
Need help translating my western blot protocol into every language - (reply: 3)
Where does mouse BRCA1 migrate on a western blot? - (reply: 1)
Western blot to CD44? - (reply: 3)
Suggestion for anti CD44 antibody for WB and IP? - (reply: 1)
Problems with western membrane stripping - (reply: 2)
western blot of cardiomyocytes - (reply: 2)
Western blot using blood plasma - (reply: 5)
Smear formed in western blots while using tubulin as control - (reply: 4)
How to confirm the band of interest in Westerns blot - (reply: 6)
Western Blot-No Sample Band, but Positive Control band visible - (reply: 10)
Western after ChIP - how to do that? - (reply: 2)
Western not working - (reply: 4)
fused bands of beta-actin in western blot - (reply: 2)
Suggestions - Nice Western Blot Image from Office Scanner? - (reply: 7)
Problem with GFP detection in western - (reply: 6)
Wrong protein size on western blot - (reply: 10)
How to know if the signal of western blot is saturated ? - (reply: 4)
Uneven banding - Western blot troubleshooting - (reply: 4)
Western Blot with fixed cells - (reply: 5)
His Tagged Recombinant Protein not Recognized in Western Blot - (reply: 1)
Western Blot - Quantifying Tumor samples from two different blots - (reply: 2)
Protocol for detecting soluble, secreted proteins on a Western? - (reply: 1)
3 extra bands show up on my western after an IP - (reply: 2)
about western blot analysis - (reply: 1)
Western Blotting loading control for large proteins - (reply: 2)
Western Blotting of Amyloid Beta - (reply: 3)
Western Blotting of LRRK2 - (reply: 1)
Protein of 460 kD on western blot - (reply: 3)
Minimum volume antibody solution for Western blot in tray? - (reply: 4)
NCOR1 western - (reply: 8)
Discrepancy between WB and Flow cytometry result - (reply: 5)
Western blotting transfer - (reply: 2)
Western Quantification (No BCA???) - (reply: 2)
Western Blot: only non-specific bands and not protein of interest - (reply: 3)
Proper western blotting - (reply: 6)
Blank spots on WB after semi-dry, NO BUBBLES - (reply: 3)
No bands/weird bands on western blot - (reply: 3)
Western Sample Buffer - DTT vs B-mercaptoethanol - (reply: 5)
Western Blot - Protein Marker Present on Film After Developing - (reply: 1)
please help with my western blots! - (reply: 3)
(best cell lysis method for) protein expression analysis by western blot - (reply: 2)
Western blot- bands weak - (reply: 11)
Preparing supernatant from cell culture for western blot - (reply: 4)
Blocking buffer for phospho-(AKT, GSK3b) WB using Odyssey? - (reply: 1)
Curved bands in Western Blot - (reply: 2)
Western blot and immunofluorescence staining - (reply: 9)
Western blot and Triton X-100 in blocking buffer - (reply: 1)
Dot blot translation to WB - (reply: 2)
western blotting troubleshooting - (reply: 2)
Multiple Non-Specific Bands in Western Blot Detected... - (reply: 1)
EPIGENETICS : Help required ... Histone Western Blot - (reply: 1)
Far western blot: probe concentration - (reply: 1)
Western blot question- High % Gel/Low MW Protein - (reply: 7)
western blot explenation - (reply: 8)
WB of cellular proteins in human serum - (reply: 2)
Western blotting - (reply: 9)
Phosphotyrosine western blotting, HELP! - (reply: 1)
General chat about tween-20 as a WB block and wash - (reply: 1)
western blot troubleshooting - (reply: 5)
Membrane Protein SDS PAGE and WB - (reply: 1)
western blot and actin issue - (reply: 2)
Separating proteing with difference of 5Kd on western blot - (reply: 4)
Immunoprecipitated protein visible on coomassie but not detected by Western - (reply: 4)
Western Blot, Bax, Many bands - (reply: 3)
Eliminating Protein/Protein Interactions for Western Blot - (reply: 2)
Western Blot for Histone 3 K27me? - (reply: 1)
Western Blot v ELISA for Different Samples? - (reply: 1)
CCRK (CDK20) protein expression and western blot - (reply: 7)
IGF2 antibody for immunohistochemistry/WB in mouse? - (reply: 2)
SDS Page and WB: Wrong size bands - (reply: 2)
Good Scanner for Western Films? - (reply: 2)
Please help with odd bands and smears on Western blot - (reply: 4)
Western blot membrane imaging - (reply: 7)
Detection of T7 RNA polymerase by WB - (reply: 6)
Shift in the position of band onto the western blot - (reply: 3)
confusing Western results - (reply: 5)
aditional band on WB - (reply: 1)
which ELISA-based or western blot-based apoptosis method to choose? - (reply: 4)
Histone precipitation and western blotting - (reply: 6)
How to prepare blood serum for western blot? - (reply: 3)
TNF alpha and IL 1b Western Blot - (reply: 1)
Large proteins in Western - (reply: 12)
Detecting doublet bands in Western for eGFP control - (reply: 1)
Phospho-protein vs total protein Western blot - (reply: 5)
Western blot trouble shooting - (reply: 3)
Why do we use sponges in the Western Transfer? - (reply: 5)
No WB bands for H2AX at all...! - (reply: 10)
any tips for doing Fluorescence Western Blot? - (reply: 2)
Store western samples in NP-40 lysis buffer? - (reply: 1)
Need help for prepping western samples with NP-40 - (reply: 7)
Why can't we detect multiple protein in a single membrane using western blot - (reply: 2)
Non-Reduced Western Blot - (reply: 5)
can coommassie stained gel be western blot - (reply: 5)
Wet transfer: western blot - (reply: 3)
Help for western blot wet transfer blurry bands - (reply: 2)
Uneven lane widths during western blots - (reply: 10)
Using human serum as first antibody in Western/ELISA - (reply: 3)
Strip Western Blotting Troubleshooting - (reply: 2)
Fold-changes different when quantifying same protein via IF and Western - (reply: 1)
A possible protocol for western blotting troubleshooting? - (reply: 1)
Freezing cell lysates for westerns - (reply: 2)
Western blot narrow bands - (reply: 1)
Grainy image and no signal on Western using ChemiDoc - (reply: 6)
Western Blot Antibody Epitope - (reply: 1)
Western blot band anaylisis - (reply: 3)
turboGFP Ab for EGFP detection in WB, posible? - (reply: 1)
Problems with western blot detection - (reply: 1)
Using serum as the sample in Western blot - (reply: 3)
Puzzeling western blot results, missing band - (reply: 3)
western blood immunodetection Ab - (reply: 1)
stripping buffer for western blot - (reply: 3)
Rapid western blot buffer ?? - (reply: 2)
Western Blot problems-vertical streaking - (reply: 3)
Problems with high molecular weight protein transfer in western blot - (reply: 1)
Trouble Western Blotting a 360kDa Protein - (reply: 5)
pERK and pAKT in MDA-MB-231 cells by western blot! - (reply: 1)
many bands Western Blot - (reply: 2)
Western blot does not match IF - (reply: 2)
Far western blotting - (reply: 5)
Western blot mystery - possibly a sample preparation problem? - (reply: 11)
Western blot sample loading question - (reply: 1)
sample precipitation problem in western blotting - (reply: 2)
Can we use ELISA capturing antibody for Western Blot Primary antibody? - (reply: 1)
MMP-9 westernblot extra bands at 25kda and 60kda - (reply: 6)
Getting too Many Bands on my Western Blot - (reply: 9)
Western transfer tank heating up - (reply: 2)
Simple Western = a western blot revolution? - (reply: 2)
Problem with merged bands within western blot caused by amount of protein loaded - (reply: 7)
Western Blotting - (reply: 10)
brain and colon tissue homogenate for WB - (reply: 3)
Detection of glycosylations on Western blotted proteins - (reply: 2)
Problems with protein estimation and western blots - (reply: 1)
western blot loading control - (reply: 2)
Birdlike bands/lanes of SDS-Page and WB - (reply: 2)
Analyzing Western Bands by Densiometry - (reply: 2)
Can FLAG IP, but cannot detect with Western - (reply: 4)
Cloned sequence is not predicted size by western blot - (reply: 1)
Redoing only the Secondary Antibody staining on a Western - (reply: 1)
Western blot and isoelectric point - (reply: 1)
Western Blot Developing Cassette - (reply: 1)
Western Blotting Resolving Problem - (reply: 3)
Western gels running super slow! - (reply: 3)
Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
WB parametric or non-parametric - (reply: 6)
Tie-2 westerns high background black lanes - (reply: 13)
Identically loaded western bands loose intensity from left to right - (reply: 3)
eIF4G western blot - (reply: 3)
smear bands in western blot - (reply: 12)
Western blots are 'semi' quantitative? - (reply: 12)
weird query for Western Blotting - (reply: 2)
Quick/One-step/automated Western Blot - (reply: 8)
Amount of antibody used in Western Blot - (reply: 1)
WB Transfer: Voltage vs. Current - (reply: 7)
Telomerase Western Blotting - (reply: 5)
Western Blotting of Proteins less than 10 kDa - (reply: 1)
Can proteins with sizes smaller than 60 kda be seen as bands in a 6% gel western - (reply: 3)
Internal Standard for western blot - (reply: 1)
Sox-2 antibody detection using Western - (reply: 10)
western blotting - (reply: 1)
Western blot not showing protein - (reply: 2)
Western Blot not showing protein - (reply: 4)
normalisation of western blot from kodak film - (reply: 1)
Separating close bands in Western Blot - (reply: 3)
Western blot on intestine samples - (reply: 2)
Western Blot for 320KD Protein - (reply: 4)
How much cytoplasmic and nuclear extracts load in a WB? - (reply: 2)
overexpression FLAG-tagged protein shows extra bands in WB - (reply: 2)
Cell Lysis and Western Blotting - (reply: 1)
Western Blot Two Proteins - (reply: 1)
Western Blot Protein Length - (reply: 4)
Western Blot no target bands, show Non-specific bands - (reply: 5)
Western Blot no signal - (reply: 6)
smearing in western blotting - (reply: 4)
Housekeeper for Plasma samples in Western Blotting - (reply: 3)
Comparison between Western blot, Immunofluorescent, Immunohistochemistry and ELI - Protein detection/separation methods (reply: 1)
Protein agregation in WB samples - (reply: 3)
what caused bubble band of my WB? - (reply: 4)
asking suggestions for western blot - (reply: 3)
Strange bands and large smear in western blot - (reply: 4)
Use of colloidal coomassie stained to western blotting?? - (reply: 5)
Dark western blot film - (reply: 12)
Bradford Vs beta-actin for WB protein quantification - (reply: 3)
Fuzzy bands in western blot - (reply: 2)
SDS Page heat up and "fry" the gel and protein (Western Blot) - (reply: 2)
I am desperated with my WB!! - (reply: 2)
western blot of insulin receptor - (reply: 1)
Preparation of mammalian cell lysis - Preparation of mammalian cell lysis for Western blotting (reply: 7)
WB - Transferring REALLY big proteins - (reply: 2)
Western Blot Statistics - (reply: 2)
Oscillation on my Western Blot results - (reply: 3)
Incubating Western in primary for longer than 24 hours at 4C? - (reply: 3)
Many unspecific bands after using anti-His on Western blot - (reply: 4)
western blot bands wider at the middle than the top - (reply: 2)
Concentrated conditioned medium for western blot - (reply: 1)
can't get bands in Western Blot - (reply: 5)
Protein Aggregation, Sample Boiling, help! - Western Blotting (reply: 4)
Converging lanes in Western - (reply: 8)
Huge background in Western blot - Studying the histone modification (reply: 1)
Actin Bands smiling on western blot - (reply: 3)
Boiling, protein aggregation......reversal?? (western) - (reply: 3)
CHOP protein western blot...help reqd! - transfer conditions for CHOP protein (reply: 3)
stripping of Western blot? - how does it work (reply: 1)
Imaging western blot membrane - (reply: 2)
a high background of western - a high background of western (reply: 2)
western blotting at high current - (reply: 1)
Western Blot of Large Protein Help - (reply: 1)
Fluorescent Western Blotting Help - (reply: 8)
Fusion tagged protein and Western Blot problem - (reply: 3)
Western blot of apoptotic cells - (reply: 2)
Quantification of proteins in Western Bands - (reply: 2)
Western didnot work should I strip blot if i want to detect same protein-Notch I - (reply: 1)
troublshooting WB - (reply: 1)
Western: Mouse antibody on mouse tissue homogenates - (reply: 3)
western blot - substarate buffer recepie (reply: 2)
Cell Lysates from a Western Blog - (reply: 2)
Backgrounds persistence and higher than target protein in western blot - Expressed in BL21(DE3) (reply: 14)
The Good Antibodies for Western Blots Thread - where people share info on antibodies that work well (reply: 3)
how to remove aspecifics on western blot filter - (reply: 2)
How do you identify protein detected on western blot from a lysate???? - (reply: 2)
western without tween - (reply: 3)
PI needs something ASAP! - densitometry on westerns (reply: 1)
Primary Mouse antibody on mouse tissue - How to use mouse antibody to do western on mouse protein (reply: 2)
Help! Cell homogenization and protein extraction for Western Blotting - (reply: 2)
Western for very small protein - (reply: 3)
microRNA knockdown its target by western blot but can't inhibit the 3'UT - want a reliable protocol to do the 3'UTR luciferase assay (reply: 3)
Western Blot Secondary Antibody Diluent - basic question (reply: 3)
Blue Native PAGE - Problems with westerns of Blue Native Gels (reply: 3)
Running a western with cell media in gels? - (reply: 11)
LC3 antibody advice - LC3 western blot (reply: 4)
Ammonium sulphate precipitation Problem - Precipitants couldnt be seen on SDS-PAGE and WB (reply: 9)
Western unresolved lanes - (reply: 2)
Doing WB from scratch for the first time. Need some info. - (reply: 2)
Western blot bovine collagen typ I problem - (reply: 3)
ECL visualization method in WB - (reply: 1)
High background using 5% BSA for blocking Western Blot - (reply: 3)
Western difficulty in primary neuron cells. - (reply: 1)
Filming membrane (Western Blot & ECL) - (reply: 1)
low signal in WB - (reply: 3)
western blot is not consistent with immunofluorescence - (reply: 2)
Western Blot - Input vs IP - (reply: 1)
I see bands with western blot only the third day! - (reply: 2)
Western Blots on Membrane Proteins - (reply: 9)
Samples for Western to detect phosphorylation of EGFR - (reply: 2)
Nucleic Acid interference in Western Blot / Optimal Nuclear Extract Protocol - Nuclear Extract Optimization (reply: 4)
western blot - (reply: 4)
anti his antibody for western blotting - high background in cell lysate western blotting (reply: 3)
two bands detected in western - (reply: 1)
Strange Western blot background - (reply: 2)
loading buffer and western blot help! - (reply: 5)
"Sandy WB" - (reply: 9)
No protein lane have bands on Coomassie and western blot?! - (reply: 4)
Strange SDS gel after Western Blotting - (reply: 1)
Western blot data analysis - (reply: 1)
Western blot fail, time and time again - (reply: 21)
Western blot of urine samples - Problems verifying loading (reply: 1)
IL-6 antibody - Which IL-6 antibody to use in Wb (reply: 2)
Western blot transfer weak - (reply: 1)
Quantitative Western Imaging - (reply: 3)
dot blot works, western doesn't - HELP! - (reply: 5)
nicotinic receptor western blot - a3 nAChR band at 100kDa instead of 55kDa (reply: 2)
too much bands in western - (reply: 2)
western blot to a metal mesh - (reply: 3)
PVDF membrane dried after transfer (Western) - (reply: 6)
Western blot problems - No or vey little transfer, expanding gel in transfer (reply: 5)
No band after WB revelation - (reply: 2)
how to check histone in western blot - (reply: 1)
western blot woes - (reply: 2)
Weak western signal for help - (reply: 13)
Western not working anymore - (reply: 15)
Western few questions - (reply: 1)
Western Blotting of Nuclear Extracts - Does high salt inhibit western blotting? (reply: 3)
Antibody works in immunofluorescence, but not in WB - (reply: 4)
B-actin as western blot -control - (reply: 4)
horrific western blots , please help - (reply: 3)
Collagen II western blotting - (reply: 1)
Western blot: problem with protein migration - trouble shooting......... please help! (reply: 10)
Western Blotting - Strange results (reply: 3)
Does the tagged-protein always appear at the predicted size? - on western blot (reply: 1)
western bands look funny - (reply: 5)
HELP! WESTERN BLOTS SUDDENLY STOPPED WORKING! - (reply: 3)
Western blot running and loading samples - (reply: 6)
problems with His tagged protein western blots - (reply: 3)
HELP! Western Blot: Anti His detection problem - Why cant my His tag be detected? (reply: 1)
Different TBST pH's for WB incubation, why? - (reply: 1)
Western Blot for low expressed protein - (reply: 4)
Denaturing Reagent for Western Blot - (reply: 16)
western exposure one part of band darker - (reply: 6)
High protease activity in protein extraction - A problem in western blot (reply: 2)
Film for Western Blots - (reply: 2)
Western Troubleshooting Help, please! - (reply: 3)
Re-use membrane in Western Blot - (reply: 3)
Dot blot- great, western blot - no signal - (reply: 3)
problem in western blotting, picture attached - spots in xray film but no bands (reply: 4)
3x flag tagged cloning -> western -> multiple bands but not non-specific - Please help me!! (reply: 1)
basic question in SDS and western blot - (reply: 1)
Analyzing outer mitochondrial membrane proteins via WB - (reply: 6)
Western blot materials - sealed plastic (reply: 2)
anti-mouse CD11b in WB - Has anybody used an Ab aganist CD11b that works in WB? (reply: 2)
Annoying occasional western problem ... anyone have any explanation? - (reply: 4)
ugly merged bands in western blot - (reply: 3)
bad western blot! what is the cause? - (reply: 3)
Western Blot Data Analysis - (reply: 2)
Western blot for muscle - (reply: 2)
Identify the band in western blot - (reply: 5)
Need help with Caspase WB - (reply: 2)
Western Blot Antibodies for inhibiting protein activity?? - (reply: 2)
Detecting BDNF with WB... - troubleshooting... (reply: 2)
Help me convince my boss... - Calling all western blot gurus! (reply: 3)
save me with the western blot to detect phosphorylated Jak2 and STAT4 - (reply: 3)
western blot using culture media - (reply: 4)
Re-inserting marker into scanned WB - (reply: 1)
Functions of BSA and Tween 20 in western blotting - (reply: 2)
develop western by ECL plus - (reply: 1)
Western with conjugated antibody? - (reply: 2)
primary antibody for western - (reply: 6)
my western blots of membrane protein do not work - (reply: 9)
Western Bizarre - Is it the lack of SDS cause the twisted and partially diffused bands？ (reply: 9)
About GFP- tagged proteins in western blot - (reply: 1)
WESTERN BLOT OF LARGE AND MINORITY PROTEIN - (reply: 1)
western blot sample in high salt concentration - (reply: 6)
Transfection and WB - Transfected cell line shows incorrect bands on WB (reply: 2)
Secondary Antibody reacting with Loading Buffer? Is this possible? - Having problems with unidentified bands in western blot (reply: 3)
how to get rabbit antibody for Western blotting - (reply: 2)
western blot - inconsistent western blot (reply: 6)
voltage/amper problem in western blot - Is there a link between volts (or mAm)and resolution? (reply: 1)
Western blotting - (reply: 4)
Use of cold milk??? - Western blot (reply: 2)
Western blotting for proteins in vesicles? - (reply: 2)
Help with Western Blotting of Keratin Proteins - (reply: 2)
Western bands fading out, pleeeese help - (reply: 1)
western blot w/ anti-FLAG ab - multiple bands show up (reply: 7)
Protein won't transfer for Western Blot - (reply: 5)
cytochrome C Western Blot - (reply: 7)
Looking for Abtibody Suppliers/Manufacturers - Hycridoma, monoclonal, manufacture, Antibody, mAb, ELISA, Western Blot (reply: 2)
IFS is positive and WB negative - (reply: 1)
immunofluorescence and western blotting - (reply: 3)
Immunoblotting - (reply: 7)
storing WB blots in PBS-tween - (reply: 7)
Fluorescent visualization of Western blot - (reply: 2)
Protein present in Western, not ELISA - (reply: 10)
PDI western blot - can't reduce the disulfide bonds (reply: 5)
the sensitive amount of protein for western blotting - (reply: 3)
How to determine final amount of 20 ng protein per WB well - (reply: 8)
Western blotting and immunofluorescence results don't correspond to each oth - (reply: 2)
protein size change after western blot - (reply: 1)
Western blot protocol - (reply: 2)
Tips for Western Blot of Low Molecular Weight Protein (HCV core protein) - (reply: 1)
Native PAGE w. Western protein marker - (reply: 4)
Can my sample be going to the buffer instead of the membrane?? - 17kDa protein not showing in WB, transfer is fine though. (reply: 6)
Problem transferring HIV-1 tat protein to a PVDF membrane for western blot - (reply: 5)
western blotting problem with detection - signal fades away quickly (reply: 2)
Two bands for p53 on Western Blot - (reply: 7)
Overblocking Western? - (reply: 5)
Preparation of tree tissue for western blot - (reply: 1)
NFkB western blotting - (reply: 3)
DyLight vs. IRDye Western Blot Li-Cor Odyssey - Flourescent labled antibody DyLight vs IRDye (reply: 1)
Western Blot for Bacteria - Loading Control (reply: 1)
uggly, non-focus and diffuse bands in my Western.. - Can you see my image of my Western to LC3? (reply: 1)
How to solve this western bolt problem? - dumbbell-shape bands (reply: 2)
Western blotting question - How to strip a mambrane and how many times can it be used (reply: 7)
IP and western blot - (reply: 5)
western blot - problem regrding western blotting (reply: 1)
ImageJ vs. LI-COR Odyssey - Quantify western blot results (reply: 1)
Nothing appeared at all in my Western! - Followed all the protocol, but nothing appeared. (reply: 7)
WB reference - Good reference for WB (reply: 6)
western blot problem: cell culture VS mouse tissue - (reply: 4)
Equal loading for Supernatant western blots??? - (reply: 1)
western blotting loading control - (reply: 5)
Using Photoshop etc on Western bot images - (reply: 8)
Ghost bands in western blot - Western blotting problems (reply: 9)
Why immunoblotting of actin coming at higher Molec. Weight - (reply: 1)
x-ray films vs direct imaging - Preferred method for western development (reply: 17)
beginner's Questions on Western Blotting - (reply: 4)
White speckles on western blots - Why?? :( (reply: 3)
Weak signal in WESTERN BLOT - Western Blot problem (reply: 1)
SDF-1(CXCL12) western blot - (reply: 13)
western blot - western blot (reply: 17)
handling retinal tissue for western blot analysis - (reply: 4)
Problems with my western blotting of LC3, autophagy protein... - (reply: 6)
actin MW problem in western blot - (reply: 4)
A single band on WB smaller than expected - (reply: 3)
WB does not correspond to protein array - (reply: 1)
Quantifying Western blots - (reply: 1)
western blot stripping buffer - please recomend a stripping buffer for re-bloting (reply: 2)
Strange bands in western of RPE-1 cells - (reply: 1)
Looking for new endogenous control for WB - (reply: 3)
western blot problems - western blot troubleshooting (reply: 3)
Co-IP and Western blot with snap-frozen brain tissues - (reply: 2)
Western Blotting - (reply: 1)
Same Non Specific Banding on Western using HA, FLAG and GFP detection - (reply: 7)
Immunofluorescence and Western Blot don't match - (reply: 5)
Western Blot and Odyssey Image Software - (reply: 1)
western blot gamma interferon - weak signal & denaturation (reply: 4)
problem with Western blot loadig control - (reply: 2)
western blotting at 37oC? - is it possible? (reply: 1)
VEGF for western blot - (reply: 2)
starry sky western blot... lots of spots. - (reply: 5)
Western blot has alot of spots - (reply: 5)
Tips on re-use of primary antibody in Western blot - (reply: 14)
Protein much larger on Western blot than predicted - (reply: 12)
how to deal with PVDF filter after WB - (reply: 5)
western for methylated histone - (reply: 7)
gel shift in western blot - (reply: 3)
WB for phosphorylated protein complex in rat intestinal tissue - (reply: 2)
Multiple antibody blotting - western blot - (reply: 16)
Is immunoblotting possible after the gel has been silver stained - (reply: 2)
Could this liver homogenate be used for Western Blot? - (reply: 2)
Western Blotting- A beginner Guide - Abcam (reply: 3)
PVDF vs. Nitrocellulose for Western blot - (reply: 20)