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PCR Related Discussions

1. Why are my genotyping PCR bands blurry? - (reply: 4)
   
2. Calculation of copy number for real time pcr - (reply: 1)
   
3. Light genotyping PCR bands - (reply: 4)
   
4. end point PCR using real time PCR machine (QS5) - (reply: 3)
   
5. Primer concentration - (reply: 1)
   
6. Efficiency curve of primers - (reply: 1)
   
7. Designing Primer for PCR - (reply: 1)
   
8. How to publish real time PCR results? - (reply: 2)
   
9. issue with PCR - (reply: 1)
   
10. ITS gene PCR Smears - (reply: 6)
    
11. Trouble with PCR after cloning - (reply: 1)
    
12. real-time PCR machine error - (reply: 1)
    
13. bands are not discrete in PCR, why? - (reply: 1)
    
14. Weak band in negative control in PCR - (reply: 5)
    
15. Verifying positive during PCR amplification - (reply: 1)
    
16. Why do I get two bands on the gel of a digested PCR product after purification w - (reply: 1)
    
17. Favorite polymerase for general cloning? - (reply: 3)
    
18. restriction enzyme pcr cloning - (reply: 1)
    
19. Weird PCR results - (reply: 2)
    
20. Primer design (Real time PCR) - (reply: 2)
    
21. Exact amplification in negative control??? - (reply: 1)
    
22. PCR is inconsistent - (reply: 4)
    
23. Specific primer problem - (reply: 2)
    
24. Bisulfite conversion, PCR end of the rope - (reply: 3)
    
25. How to minimize delta ct value when everything else worked well in qRT PCR - (reply: 1)
    
26. How to minimize delta ct value when everything else worked well in qRT PCR - (reply: 1)
    
27. Faint band and primer dimer in PCR - (reply: 1)
    
28. RAPD PCR - (reply: 2)
    
29. Long pcr frustration and problem.. all help is Much appreciated - (reply: 3)
    
30. Odd issue converting mutiplex PCR to single reactions - (reply: 3)
    
31. How to check if my primers were degraded? - (reply: 2)
    
32. problem with PCR amplification of cDNA - (reply: 4)
    
33. Funny shaped PCR curve - (reply: 1)
    
34. digital PCR - (reply: 1)
    
35. Length of amplicon for RT real time PCR - (reply: 2)
    
36. REAL TIME PCR WITH TAQMAN PROBE - (reply: 1)
    
37. Optimal dNTP storage pH - (reply: 1)
    
38. Design of <=40bp primers adding restriction sites and also Gibson Assembly o - (reply: 1)
    
39. Primer design HELP! - (reply: 4)
    
40. Amount of cDNA input in RT-PCR - (reply: 1)
    
41. PCR cycler with end product fluorescence detector - (reply: 5)
    
42. Housekeeper Genes and Real-Time PCR - (reply: 1)
    
43. Pre-designed qRT-PCR primers - (reply: 1)
    
44. PCR positive control of antibiotics resistance gene - (reply: 1)
    
45. Same primers and different melting peaks for two samples (~1°C appart) - (reply: 2)
    
46. Designing primers with his-tag for pET28a+ vector - (reply: 3)
    
47. High molecular weight band from my PCR - (reply: 3)
    
48. Can someone help me on my primer design? (contains flag tag) - (reply: 2)
    
49. testing primers efficiency for rt-PCR - (reply: 1)
    
50. -RT contamination problem in PCR - (reply: 5)
    
51. qPCR std curve calculation issue if using PCR product as the std - (reply: 1)
    
52. Thermal Cycler for Multiplex PCR - (reply: 3)
    
53. Problem of melt curve shape for pikoreal real time PCR - (reply: 1)
    
54. Primer sequences with M and Y - how to order? - (reply: 2)
    
55. RT-PCR low efficiency - (reply: 1)
    
56. emulsion PCR - (reply: 1)
    
57. RT-PCR electrophorasis - (reply: 1)
    
58. PCR contamination - (reply: 6)
    
59. PCR bands on gel - (reply: 5)
    
60. Problem with restriction digestion of cloned PCR product - (reply: 5)
    
61. SYBR Green Real time PCR - (reply: 2)
    
62. What would be the shortest and optimal method of extracting human cells for PCR? - (reply: 2)
    
63. need help calculating stock primer concentration - (reply: 1)
    
64. High background fluorescence detected before starting PCR - (reply: 3)
    
65. How to clean up PCR place & pipettes? - (reply: 1)
    
66. Real-time pcr - (reply: 1)
    
67. Using qPCR machine for simple PCR, is it possible? - (reply: 3)
    
68. PCR inhibitors - (reply: 1)
    
69. What could be the possible reasons for a RT-PCR experiment that was working fine - (reply: 4)
    
70. Cleaning loading dye for PCR sequencing - (reply: 1)
    
71. Polymerase mixture for blunt ended fragments - (reply: 2)
    
72. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)
    
73. Amplifying shRNA lentivirus - (reply: 1)
    
74. Mineral oil quality in PCR tubes - (reply: 5)
    
75. Diagnostic PCR troubleshooting - (reply: 2)
    
76. RT-PCR Kit's Validation - (reply: 1)
    
77. Rt pcr - (reply: 4)
    
78. Inhibition of PCR amplification of bacterial genomic DNA by RNA - (reply: 1)
    
79. methylation specifc primers thermal cycle conditions - (reply: 4)
    
80. PCR Amplification Issues and Primer dimer - (reply: 4)
    
81. How to make PCR analysis using the 22DDCT Method - (reply: 1)
    
82. Primer calculation for qPCR - final reaction - (reply: 1)
    
83. Sec. structure in primers - (reply: 1)
    
84. Changing PCR recipe halfway - (reply: 1)
    
85. PCR master mix containing primers and Taq - (reply: 4)
    
86. Primer working with some cDNA samples and not other - (reply: 2)
    
87. no bands after pcr!!!! - (reply: 4)
    
88. Experimental design for RT-PCR - (reply: 8)
    
89. Primer dilution - (reply: 9)
    
90. why not working msp primers - (reply: 4)
    
91. PCR product not migrating from wells...?? - (reply: 6)
    
92. Re-PCR my PCR products - (reply: 2)
    
93. PCR DIG-labeling kit preblem? - (reply: 1)
    
94. pcr - (reply: 1)
    
95. how to analysis the BiSearch ePCR result - (reply: 3)
    
96. Standardizing cDNA concentration before doing PCR - (reply: 4)
    
97. Volume of Primers - (reply: 4)
    
98. PCR conditions unknown? - (reply: 10)
    
99. PCR anomaly in a mid-range dilution of the template - (reply: 2)
    
100. Reducing contamination in 16s PCR for metagenomic library prer - (reply: 4)
     
101. MSP_Primer dimers? - (reply: 17)
     
102. PCR using very long oligos !!! - (reply: 4)
     
103. Primer Designing - (reply: 9)
     
104. Housekeeping gene for Real Time PCR when comparing expression of genes across di - (reply: 1)
     
105. Colour of PCR gel band best for ImageJ analysis - (reply: 2)
     
106. colony pcr, two strong bands - (reply: 4)
     
107. Ligation of two PCR products - (reply: 1)
     
108. pcr primer design - (reply: 6)
     
109. Please help (primer/ probe final volume calculations) - (reply: 1)
     
110. Primer design with EcoRI ends - (reply: 2)
     
111. Amplification problem in DNA isolated after ChIP experiment, - (reply: 1)
     
112. Trouble with PCR using ligation mix as template? - (reply: 3)
     
113. PCR with mammalian cells as template - (reply: 1)
     
114. PCR with overlapping primers only (primer extention) - (reply: 3)
     
115. Random chuck of nonsense in middle of PCR - (reply: 6)
     
116. RT-PCR analysis - (reply: 1)
     
117. PCR DNA bands too large on agarose gel - (reply: 1)
     
118. MSP Primer design - (reply: 5)
     
119. New to PCR - (reply: 3)
     
120. How can I check the quality of bacterial primers and their specificity on blast? - (reply: 1)
     
121. 3-primer PCR Genotyping - problem in hemizygotes - (reply: 2)
     
122. Primer design - (reply: 1)
     
123. What % of colon cancer patients does a general colon PCR array detect - (reply: 1)
     
124. Primer non specific binding sites - (reply: 7)
     
125. Degenerate primers - (reply: 8)
     
126. Primers with 2 different annealing temp - (reply: 1)
     
127. Can you use same samples for real time pcr 2nd time? - (reply: 2)
     
128. Staphylococcus aureus colony PCR - (reply: 1)
     
129. Cloning purified PCR product into cells - (reply: 5)
     
130. PCR & Western Blot sample preperation - (reply: 2)
     
131. PCR & Western Blot sample preperation - (reply: 5)
     
132. Y-linker transgene integration site PCR primer verification - (reply: 1)
     
133. Non especific band in PCR... - (reply: 2)
     
134. I need help with PCR problems - (reply: 5)
     
135. Question about baselines in Real-Time PCR - (reply: 3)
     
136. Inconsistent colony PCR result - (reply: 2)
     
137. A problem with PCR array - (reply: 4)
     
138. My PCR suddenly stopped working and I'm losing my mind - (reply: 3)
     
139. Concentration of Eva green with different amplicon sizes in a PCR pool - (reply: 1)
     
140. labX offers on realtime PCR machines - (reply: 4)
     
141. Could any of you help with an explanation to these vague real time PCR curves &# - (reply: 2)
     
142. polymerase with or without proofreading activity used in study nucleotide polymo - (reply: 1)
     
143. how to calculate amount of cDNA using RT-PCR - (reply: 2)
     
144. Suggest best micropipette for PCR purpose... - (reply: 1)
     
145. NEB TM CALCULATOR PRIMER CONCENTRATION - (reply: 2)
     
146. Plasmid Amplification Issue - (reply: 6)
     
147. PCR product and Restriction products bands are not of the expected size - (reply: 4)
     
148. primer design for a gene - (reply: 3)
     
149. large bands in all pcr reactions including negative control - (reply: 1)
     
150. confirmation for primers dilution - (reply: 10)
     
151. Problems regarding amplification of my gene - (reply: 3)
     
152. PCR working, qPCR is *not*. - (reply: 6)
     
153. DIY PCR Cleanup/Gel extraction and miniprep solutions - (reply: 2)
     
154. Overlapping PCR, need help - (reply: 5)
     
155. How does RT-PCR work? - (reply: 5)
     
156. Denatured plasmid for pcr - (reply: 1)
     
157. Possible primer dimer problem..Need solution!!! - (reply: 2)
     
158. confusing with where to put primers and which ordination - (reply: 4)
     
159. Help with primer concentration for sequencing - (reply: 1)
     
160. PCR troubleshoot - (reply: 6)
     
161. Some info. on PCR products stability w/respect to shipping - (reply: 3)
     
162. PCR Products not run well in the GEL - (reply: 1)
     
163. PCR solution calculus - (reply: 4)
     
164. Amplifying from pcr products - (reply: 7)
     
165. Consistancy issue with PCR block - (reply: 1)
     
166. Queries regarding PCR - (reply: 3)
     
167. Design the primers - (reply: 10)
     
168. Calculation of Taq Polymerase - (reply: 3)
     
169. Best Taq for colony PCR? - (reply: 5)
     
170. How two fragments joint together when doing fusion PCR? - (reply: 4)
     
171. How to test my milleq water in q PCR about contaminants - (reply: 3)
     
172. Mutagenesis PCR - (reply: 9)
     
173. real time PCR primer design - (reply: 1)
     
174. The PCR gods are frowning upon me - (reply: 9)
     
175. PCR after T4 ligation? - (reply: 3)
     
176. primers for cloning - (reply: 1)
     
177. Degenerated Primers and their problems - (reply: 1)
     
178. Sonication of small PCR amplicon - (reply: 1)
     
179. primer design with restriction enzyme - (reply: 1)
     
180. Interpreting primer BLAST scores for self-complementary - (reply: 5)
     
181. primer dilution help - (reply: 1)
     
182. Testing primers on 'unknown' tissue - (reply: 1)
     
183. How to remove inhibitory substances in PCR? - (reply: 2)
     
184. need some advise about PCR of PKD1-human gene - (reply: 7)
     
185. puzzled with PCR outcome after BS treatment - (reply: 2)
     
186. nested PCR for low viral load- HBV patient sample - (reply: 2)
     
187. troubleshooting stubborn PCR - (reply: 6)
     
188. Measuring pcr fragments in a gel - (reply: 1)
     
189. Amplification in HK gene but not for target gene - (reply: 3)
     
190. could you help me with my stem loop RT primer? - (reply: 1)
     
191. What is the difference between Hot start polymerase and the taq polymerase - (reply: 3)
     
192. How long can I store at - 30°C my PCR mix? - (reply: 6)
     
193. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
     
194. RT-PCR help - (reply: 2)
     
195. Autoclaving PCR waste in the room where PCRs are set up and run - Is it a proble - (reply: 2)
     
196. PCR Temperature change control malfunctioning - (reply: 1)
     
197. excess amount of primers - (reply: 2)
     
198. Dehydrating and Reconstituting primers - (reply: 15)
     
199. Trouble with PCR of short sequence - (reply: 3)
     
200. Setting the temperature range for gradient PCR - (reply: 3)
     
201. KIR gene promoters for MSP primers design - (reply: 2)
     
202. Biotin-streptavidin signal amplification - (reply: 2)
     
203. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
     
204. Amplification curve in the negative control samples - (reply: 7)
     
205. PCR of bisulfite converted DNA is now producing a smear? Previously produced a s - (reply: 2)
     
206. PCR troubleshooting: wrong amplicon size when spiked into sample - (reply: 4)
     
207. Electrophoresis after PCR : too many bands - (reply: 6)
     
208. Troubles with PCR (Rosa26 locus in mouse line) VERY CONFUSING! - (reply: 6)
     
209. Primer as limiting reagent in PCR reaction - (reply: 2)
     
210. Primer Design for RNA probes - (reply: 2)
     
211. Real time PCR for degraded RNA - (reply: 1)
     
212. Microfluidic PCR Issues, Master Mix and Consumable Variations? - (reply: 5)
     
213. Doubled polymerase in the rxn, does this increase pcr efficiency? - (reply: 1)
     
214. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
     
215. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)
     
216. Nested BSP Primer Design - (reply: 6)
     
217. website for primer design - (reply: 1)
     
218. PCR Efficiency over 150%! - (reply: 1)
     
219. problem of amplification - (reply: 2)
     
220. PCR not working - (reply: 11)
     
221. How to do a primer dilution - (reply: 10)
     
222. Troubles with Fusion PCR - (reply: 1)
     
223. Primer design and alternative transcripts - (reply: 2)
     
224. PCR and sequencing of genomic DNA - (reply: 5)
     
225. qPCR amplification - (reply: 4)
     
226. NESTED PCR - (reply: 6)
     
227. Extremely desperate noob question: How do these PCR work? - (reply: 6)
     
228. RT-PCR carry over contamination and dUTP/UDG - (reply: 4)
     
229. problems regarding amplifying a 1.7 kb mRNA seq - (reply: 3)
     
230. Digestion necessary after PCR? - (reply: 9)
     
231. Inverse PCR product selection - (reply: 2)
     
232. RT-PCR - High Ct Values and Laser Capture - (reply: 1)
     
233. PCR with no bands showing in 1.1 % gel electrophoresis - (reply: 4)
     
234. how to hasten real-time PCR amplifications - (reply: 2)
     
235. Please please help me with my Phusion PCR. - (reply: 5)
     
236. PCR - (reply: 1)
     
237. NCBI Primer Design - Stringency Issues - (reply: 3)
     
238. Different primer concentration in qPCR - (reply: 1)
     
239. Multiple melt curves for primer in a sample that does not express gene - (reply: 6)
     
240. help in long pcr - (reply: 1)
     
241. PCR inhibitor in template DNA - (reply: 3)
     
242. Why when disigning primers for RTPCR, you need to chose a region where introns a - (reply: 1)
     
243. Problem with Real-time PCR results analysis - (reply: 1)
     
244. Primer Specificity: Testing only one primer - (reply: 4)
     
245. PCR from protozoa DNA - (reply: 3)
     
246. Ligation-mediated PCR: Vent Polymerase, Why? - (reply: 7)
     
247. tool for comparing many primers pairs - (reply: 4)
     
248. PCR that leads to protein synthesis - (reply: 18)
     
249. Real-time PCR does not work, while the conventional PCR (with same conditions an - (reply: 3)
     
250. Use of DMSO in General PCR - (reply: 1)
     
251. PCR product size confusion - (reply: 3)
     
252. Pcr primers - (reply: 7)
     
253. Concentration specification in PCR - (reply: 3)
     
254. Guanidine isothiocyanate in PCR - (reply: 1)
     
255. Primers have worked well but now getting primer dimers? - (reply: 2)
     
256. I cannot design primers on exon-exon junction - (reply: 2)
     
257. DNA Quantification of PCR Products - (reply: 2)
     
258. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
     
259. Problem for PCR - (reply: 9)
     
260. Designing primers in UTRs - (reply: 1)
     
261. Multiplex PCR - (reply: 1)
     
262. Whole mtDNA genome amplification with long-range PCR...trouble - (reply: 7)
     
263. primer design@ buy? - (reply: 2)
     
264. Trouble with overlap extension pcr - (reply: 3)
     
265. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
     
266. designing primers( selecting target sequence/amplicon design) - (reply: 3)
     
267. primer checking & restriction enzyme based methylation specific polymerase c - (reply: 8)
     
268. Question about the RT PCR - (reply: 3)
     
269. faint dna band after pcr purification - (reply: 1)
     
270. PCR ready mixes with long shelf lives - (reply: 4)
     
271. Troubleshooting: Inverse PCR - (reply: 3)
     
272. How to design primer to amplify genomic DNA? - (reply: 3)
     
273. Overlap PCR, need help - (reply: 11)
     
274. Internal control for miRNA RT-PCR - (reply: 1)
     
275. Primers - (reply: 1)
     
276. Primers mix - (reply: 2)
     
277. question about RNA concentration for real time PCR - (reply: 1)
     
278. Need help with dCAPS pcr, seeing huge bands on gel - (reply: 1)
     
279. amplification for microarray analysis - (reply: 1)
     
280. PCR products sizes and DNA ladder - (reply: 7)
     
281. Having problem with primers for qPCR - (reply: 4)
     
282. Troubleshooting methylation primers for Bio-Rad PCR - (reply: 3)
     
283. PCR product sequencing - (reply: 3)
     
284. Bad fragment amplification - (reply: 4)
     
285. Can somebody explain to me what "spiking" means in RT-PCR and why do you - (reply: 3)
     
286. Bisulfite Sequencing and PCR Troubleshooting - (reply: 2)
     
287. No amplification with TRAPEZE kit! - (reply: 1)
     
288. PCR Purification or Gel Extraction for Southern Blot - (reply: 5)
     
289. Stargazer PCR problems - (reply: 3)
     
290. General PCR discussion - (reply: 8)
     
291. Real time. No amplification but hight flourescence - (reply: 1)
     
292. Real time PCR doubt - (reply: 5)
     
293. PCR Profile for ligation - (reply: 3)
     
294. protocol to relieve melanin inhibition of PCR - (reply: 4)
     
295. No bands despite different primers and conditions and TAqs - (reply: 8)
     
296. Multiplex TaqMan-like Assay PCR Efficiency - (reply: 3)
     
297. Equimolar Mix Primer - (reply: 8)
     
298. Wrong PCR product - (reply: 2)
     
299. Colony PCR positive and Digestion negative????? - (reply: 11)
     
300. Primer design - (reply: 5)
     
301. storage for lyophilized primers - (reply: 1)
     
302. PCR GAPDH gene - (reply: 1)
     
303. Repeated mutagensis primer in site-directed mutagenesis - (reply: 8)
     
304. Quantitative RT-PCR statistics help - (reply: 1)
     
305. Dimerization of PCR product - (reply: 4)
     
306. PCR Primer trouble - (reply: 2)
     
307. Question about taq polymerase for multiplex PCR prior to NGS using Illumina tech - (reply: 1)
     
308. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
     
309. is it necessary to introduce mismatches in the inner primers of tetra primer ARM - (reply: 1)
     
310. finding the corrosponding primers - (reply: 2)
     
311. Gene sequence for Real time PCR - (reply: 2)
     
312. non-reproducible PCR results with cDNA as template - (reply: 1)
     
313. Ligation of PCR fragments - (reply: 11)
     
314. Information about the use of DNA diluted in Real Time PCR - (reply: 6)
     
315. RNA extraction for RT-PCR - (reply: 3)
     
316. PCR failing for transformed plant DNA but not for Agro - (reply: 3)
     
317. problems with gDNA doing real time PCR in yeast - (reply: 2)
     
318. Design PCR primers for cloning 3 copies of same insert - (reply: 2)
     
319. Designing primers for a nuclear region to amplify in a species that has no nucle - (reply: 1)
     
320. How to get rid of bands in PCR negative control - (reply: 10)
     
321. T7 and M13 primers two band amplification - (reply: 3)
     
322. Protocol for qPCR using the ABI SYBR® Green PCR Master Mix - (reply: 1)
     
323. Strange PCR problem - (reply: 2)
     
324. Question about Double Digestion followed by PCR amplification - (reply: 2)
     
325. PCR problem - (reply: 2)
     
326. PCR machine not working properly - (reply: 1)
     
327. fluorescent primer vs fluorescent terminator in sequencing - (reply: 4)
     
328. PCR gene specific amplification problem - (reply: 3)
     
329. Failure SYBRGREEN PCR - (reply: 4)
     
330. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
     
331. without RE site in PCR product - (reply: 5)
     
332. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
     
333. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
     
334. real-time pcr non reproducible - (reply: 4)
     
335. Strange amplification plots with high Ct variability - (reply: 1)
     
336. How big a role does mixing play in PCR - (reply: 1)
     
337. Melting curve is irregular for primer optimization - (reply: 5)
     
338. Designing primers for ABO blood groups - (reply: 1)
     
339. a smeared Gen-DNA template ---> smear and less yield on pcr ? - (reply: 1)
     
340. RT-PCR primer design - (reply: 7)
     
341. How to amplify very short PCR template - (reply: 4)
     
342. Is this primer okay? - (reply: 4)
     
343. PCR amplification of large template - (reply: 1)
     
344. PCR insert - in frame? - (reply: 2)
     
345. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
     
346. smear in gel electrophoresis after PCR - (reply: 2)
     
347. Can I use Primer as template? - (reply: 1)
     
348. Whole plasmid amplification by PCR - (reply: 2)
     
349. PCR product as standard curve template - (reply: 6)
     
350. colony PCR after transformation - (reply: 1)
     
351. PCR machine - (reply: 3)
     
352. qPCR - no amplification curve but suitable melting curve - (reply: 3)
     
353. Designing a PCR based detection method for an unknown virus/pathogen - (reply: 2)
     
354. Difficult PCR containing triplet repeats and Frt sites - (reply: 1)
     
355. Resuspending primers calculation - (reply: 4)
     
356. PCR of GC-rich sequence (E-cadherin) - (reply: 6)
     
357. PCR RFLP is PCR product purification necessary? - (reply: 3)
     
358. Mystery in my PCR - (reply: 5)
     
359. Whole genome amplification from cDNA? - (reply: 4)
     
360. What do you use to check primer secondary structure? - (reply: 3)
     
361. Need help with my real time RT-PCR Plate Set up - (reply: 8)
     
362. PCR product as template for in vitro transcription - (reply: 1)
     
363. Problem with 3 step PCR - (reply: 3)
     
364. mutagenic primers with very high GC content. - (reply: 3)
     
365. Scaling up PCR to get more DNA - (reply: 5)
     
366. PCR to get 10kbp product - (reply: 4)
     
367. site-directed mutagenesis primer Tm - (reply: 4)
     
368. Single-step nested PCR: how to investigate dynamics? - (reply: 2)
     
369. Random vs oligo primer in preamplification RT - (reply: 1)
     
370. how does polymerase stop at the required length - (reply: 1)
     
371. Chip pcr. are there inespecifics? - (reply: 1)
     
372. Primer concentration - stupid question - (reply: 3)
     
373. Primer Design, help i´m New - (reply: 4)
     
374. No DNA after PCR product purification - (reply: 9)
     
375. Sensitivity of RT-PCR and qPCR - (reply: 4)
     
376. RT-qPCR primer problem - (reply: 4)
     
377. Mixing two cDNA samples into one for realtime PCR - (reply: 3)
     
378. real time PCR trouble - (reply: 3)
     
379. removal of ethanol in PCR product purification - (reply: 5)
     
380. Crazy real time PCR curve - (reply: 4)
     
381. [Video] Using NCBI for RT-PCR Primer Design - (reply: 1)
     
382. High fidelity PCR trouble shooting - (reply: 2)
     
383. Designing cloning primers for DNMT - (reply: 2)
     
384. Reproducible Non-Specific PCR Product - (reply: 2)
     
385. Are these primer products good enough for qPCR? - (reply: 3)
     
386. Can't get PCR with large overhang primers to work - (reply: 8)
     
387. Primer Efficiency across runs - (reply: 1)
     
388. Question about pipet tips for PCR and rtPCR - (reply: 4)
     
389. UNG in PCR - (reply: 1)
     
390. Cause of random samples failing PCR? - (reply: 2)
     
391. Detecting miRNA mimics by RT-PCR? - (reply: 4)
     
392. pcr 10X buffer preparation - (reply: 2)
     
393. how much template do i need for pcr? - (reply: 5)
     
394. will mRNA from different cell line lead to loss of expected product in RT PCR? - (reply: 2)
     
395. Problems with crossover PCR - (reply: 2)
     
396. PCR amplification with new restriction sites troubleshooting - (reply: 2)
     
397. Designing PCR Primers for cloning - (reply: 17)
     
398. Creating primers to add restriction sites to vector backbone - (reply: 7)
     
399. PCR DNA Concentration - (reply: 1)
     
400. RT-PCR product- no band - (reply: 4)
     
401. Some primers dose not work - (reply: 5)
     
402. Understanding RACE PCR - (reply: 1)
     
403. Pair of primers (SYBR) with more than one amplicon product(?) - (reply: 4)
     
404. Wierd Bands after PCR....Confused - (reply: 9)
     
405. Query regarding primers for quick change mutagenesis - (reply: 3)
     
406. PCR with Plasmid recovered from filter paper - (reply: 6)
     
407. PCR amplified product size - (reply: 5)
     
408. the storage time for primers - (reply: 9)
     
409. Annealing temperature for PCR - (reply: 8)
     
410. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
     
411. Nested PCR - (reply: 2)
     
412. random primers or oligodT - (reply: 4)
     
413. No amplification during RT-PCR - (reply: 4)
     
414. Sequencing RT-PCR product - (reply: 3)
     
415. Low yield PCR product after gel purification - (reply: 8)
     
416. To design or use published primers? - (reply: 4)
     
417. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
     
418. PCR primer usage (Clonning & cDNA) - (reply: 2)
     
419. problem in cloning PCR primer design with restriction site - (reply: 4)
     
420. Amplification with U primers, but not with M primers - (reply: 1)
     
421. Taq polymerase - (reply: 7)
     
422. His tag introduction in to gene and primer design - (reply: 2)
     
423. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
     
424. Which High-Fidelity polymerase is better? - (reply: 2)
     
425. polymerase to use for cloning - (reply: 4)
     
426. Problem amplifying viral gene - (reply: 5)
     
427. Adding tag using overlapping PCR - (reply: 2)
     
428. Tool / software for oligo analysis (hairpins, dimers etc.)? - (reply: 5)
     
429. Design primer from incomplete sequence... - (reply: 2)
     
430. Design primer from incomplete sequence... - (reply: 2)
     
431. How to perform colony PCR - (reply: 1)
     
432. Primer designing for Methylation - (reply: 6)
     
433. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
     
434. cDNA amplification problem - (reply: 4)
     
435. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
     
436. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
     
437. PCR product running on agarose gel - (reply: 32)
     
438. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
     
439. No bands in PCR after DNase treatment - (reply: 4)
     
440. PCR amplification with high fidelity enzyme - (reply: 1)
     
441. PCR HELP!!!! - (reply: 1)
     
442. Overlap PCR problem - (reply: 5)
     
443. PCR and restriction enzyme digestion - (reply: 3)
     
444. Bisulfite PCR and cloning - (reply: 5)
     
445. PCR product purification - (reply: 4)
     
446. Overlapping sequence PCR primers - (reply: 1)
     
447. restricted PCR plasmid runs slower - (reply: 2)
     
448. BSP PCR primer design explained - (reply: 11)
     
449. Bisulfite sequencing PCR worked - (reply: 5)
     
450. how to make a working solution of a primers for pcr reaction?? - (reply: 4)
     
451. Whole lab experiencing inconsistent PCR contamination - (reply: 10)
     
452. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
     
453. doing PCR using PCR product , help plz !! - (reply: 5)
     
454. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
     
455. good 16S univ. primer for qPCR? - (reply: 3)
     
456. Strange looking "elevated" amplification plots from ChIP-qPCR samples - (reply: 2)
     
457. Amplification of CAG promoter problems - (reply: 4)
     
458. Can I use Real-time PCR instead of Southern Blot to determine number of integran - (reply: 1)
     
459. Long PCR (>10kb) polymerase recommendations - (reply: 3)
     
460. I need help for designing a pair of primers for this sequence. - (reply: 1)
     
461. PCR not working for 5 Aza treated DNA - (reply: 3)
     
462. Differentiating mouse from human cells with PCR - (reply: 5)
     
463. Differentiate between RT-PCR and PCR - (reply: 3)
     
464. PCR band slightly BELOW expected length ?! - (reply: 14)
     
465. long primers PCR - (reply: 4)
     
466. unexpected band in PCR with plasmid - (reply: 4)
     
467. qPCR [template] and [primer] troubleshooting...... - (reply: 1)
     
468. DNA polymerase for GC rich template? - (reply: 5)
     
469. Sequencing Primers and Plasmids - (reply: 3)
     
470. Good Protocol for Semi-Quantitative RT-PCR? - (reply: 1)
     
471. DNA extraction - PCR Problem - (reply: 3)
     
472. Primers to amplify Kan operon from pet28 - (reply: 2)
     
473. PCR product biotinylation - (reply: 3)
     
474. Tagged primers and a second PCR targetting the tags - (reply: 5)
     
475. cloning pcr insert into plasmid - (reply: 2)
     
476. Barcode generator for PCR primers - (reply: 2)
     
477. RNA isolation with low number of cells (1*10^4 to 5*10^4) for RT-PCR - (reply: 3)
     
478. Barcoding PCR products - (reply: 18)
     
479. Biotinylated primers - (reply: 4)
     
480. role of water in PCR - (reply: 3)
     
481. HIV gene- PCR - (reply: 3)
     
482. Real time PCR melting curve - (reply: 2)
     
483. BSA in PCR reaction - (reply: 1)
     
484. Transcription of PCR products without a primer coupled with promotor sequence - (reply: 4)
     
485. Cannot see insert with colony pcr - (reply: 6)
     
486. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
     
487. isolating DNA from mouse tails and subsequent problems with pcr - (reply: 7)
     
488. How to Join two PCR products - (reply: 3)
     
489. Restriction enzyme - PCR sheep blood gDNA - (reply: 5)
     
490. What's wrong with my PCR - (reply: 5)
     
491. Tagging a gene with HA and FLAG using PCR - (reply: 1)
     
492. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
     
493. How to join two sequencing files taken form forward and reverse primer - (reply: 3)
     
494. Problem with UPL real-time PCR - please help !!! - (reply: 1)
     
495. Cp, but no amplification - (reply: 2)
     
496. pcr product digestion problem - (reply: 4)
     
497. Low PCR product - (reply: 7)
     
498. Control primers for bisulfite-converted DNA - (reply: 3)
     
499. Colony PCR - M13 vs. gene-specific primer amplification of bacmid - (reply: 4)
     
500. DNA amplification differences between samples & between regions (WGA & s - (reply: 2)
     
501. wrong dntp concentration - (reply: 2)
     
502. Third round PCR - (reply: 2)
     
503. long fragment PCR - (reply: 2)
     
504. Best proof-reading polymerase? - (reply: 13)
     
505. Is 27F and Anti-Gamma Primer the same thing for 16s? - (reply: 9)
     
506. Q regarding primer mix for MSP!! - (reply: 4)
     
507. Genomic DNA extraction - how to quantify for PCR? - (reply: 9)
     
508. Inconsistent sample quality in quantitative real-time PCR - What could be the pr - (reply: 3)
     
509. I need to break this cycle of PCR issues - (reply: 13)
     
510. Questions / Help with Fusion PCR - (reply: 8)
     
511. Controls for RT-PCR reactions - (reply: 2)
     
512. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
     
513. BSP Primers with M13 Tails - (reply: 1)
     
514. Detection of T7 RNA polymerase by WB - (reply: 6)
     
515. Multiplex PCR - (reply: 33)
     
516. issues when designing PCR primers using PRIMER3 - (reply: 8)
     
517. Primer catalog/databasing - (reply: 1)
     
518. Nonrepetitive nested PCR - (reply: 2)
     
519. Primer Blast - (reply: 6)
     
520. Help designing primers for use in In-fushion cloning - (reply: 4)
     
521. PCR internal control - (reply: 2)
     
522. Primers Freeze-Thaw - (reply: 16)
     
523. TDNA Genotyping PCR program - (reply: 1)
     
524. primer design using 16s rRNA - (reply: 1)
     
525. question about PCR and cloning - (reply: 1)
     
526. PCR - (reply: 2)
     
527. nonspecific band at 50bp in PCR - (reply: 9)
     
528. Primer concentration for cDNA synthesis using GSP - (reply: 1)
     
529. PCR master mix - (reply: 7)
     
530. Maximum PCR product Tm - (reply: 6)
     
531. what is 0.5 unit of Taq Polymerase? - (reply: 3)
     
532. cloning from cDNA but got a much shorter PCR product - (reply: 4)
     
533. PCR & Southern blotting - (reply: 1)
     
534. Bisulfite PCR Question - (reply: 2)
     
535. Primer Design HELP! - (reply: 3)
     
536. Help with sequencing of a 120bp PCR product. - (reply: 9)
     
537. Bisulfite PCR doesn't work and the primer seems abnormal - (reply: 6)
     
538. How are Primers made? - (reply: 9)
     
539. Long Non-Specific PCR Products - (reply: 5)
     
540. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
     
541. qPCR, Appearance of amplification curve for my minus RT control - (reply: 6)
     
542. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
     
543. PCR calculation - (reply: 1)
     
544. Colony PCR and digestion with KpnI - (reply: 10)
     
545. designing methylation specific primers - (reply: 3)
     
546. Help with PCR and Gel Electrophoresis. Not getting any bands - (reply: 8)
     
547. PCR - consistent false positive results - (reply: 6)
     
548. PCR no amplification - (reply: 15)
     
549. How to quantify virus by PCR - (reply: 12)
     
550. Can human 18S primer, probe set for qPCR be used for Rhesus and Cynomolgus sampl - (reply: 3)
     
551. What is a use of ΔCt and ΔΔCt in qRT PCR? - (reply: 4)
     
552. Pcr product size determination for a fusion gene - (reply: 8)
     
553. Quantify DNA before ChIP PCR - (reply: 3)
     
554. Primer Annealing Temperatures - (reply: 24)
     
555. my pcr product is larger than expected - (reply: 4)
     
556. Question about PCR - DNA or RNA as template - (reply: 4)
     
557. pcr purification - (reply: 1)
     
558. pcr purification doubt - (reply: 4)
     
559. improving sensitivity of one step TAQMAN REal time PCR - (reply: 1)
     
560. PCR band moves lower than its size on agarose gel - (reply: 3)
     
561. same primers, different product in conventional PCR and qPCR - (reply: 6)
     
562. Different primer optimalization for nested vs direct MSP? - (reply: 3)
     
563. pcr problem - (reply: 6)
     
564. NEB Q5 polymerase works very well - (reply: 1)
     
565. Primers amplify genomic DNA but not cDNA - (reply: 2)
     
566. pcr purification - (reply: 1)
     
567. storing PCR product overnight - (reply: 2)
     
568. Primer BLAST - (reply: 1)
     
569. Bands in negative control PCR - (reply: 2)
     
570. Discrepancies between miRNA Array Data and PCR Data - (reply: 1)
     
571. problem with pcr mutagenesis - (reply: 10)
     
572. HMK-Tagged PCR Cloning Problem... - (reply: 4)
     
573. Use PCR purification kit to purify restriction digestion - (reply: 2)
     
574. PCR primer design - published primers trustable? - (reply: 8)
     
575. Polymerase for E. coli screenings - (reply: 5)
     
576. complicated RT-PCR issues - (reply: 4)
     
577. question about universal and specific primers for miRNA real time pcr - (reply: 1)
     
578. Can we use degenerate bases in BSP primers? - (reply: 1)
     
579. Quick change mutagenesis No PCR product visible - (reply: 2)
     
580. Normalization of Real time PCR results using Pfaffl method - (reply: 2)
     
581. RT-PCR vs plate reader for pathogen detection - (reply: 3)
     
582. Can anyone help with my Bisulfite PCR? - (reply: 2)
     
583. How to check primer sequences using BLAST and other tools? - (reply: 2)
     
584. real time PCR analysis in patient samples - (reply: 2)
     
585. carpet in gels for PCR products - (reply: 2)
     
586. Primer Check? - (reply: 2)
     
587. Sequencing of the PCR production in BSP - (reply: 1)
     
588. amplification of 14Kb - (reply: 1)
     
589. Validation of PCR primers/ probes - (reply: 6)
     
590. Taq polymerase for MSP - (reply: 1)
     
591. Real time PCR sudenly not working - (reply: 1)
     
592. Buffer-composition One-Step RT-PCR - (reply: 4)
     
593. Two reverse primer sequences for a single forward primer - (reply: 1)
     
594. PCR with more than one primer - (reply: 1)
     
595. RAPD - PCR problem - (reply: 5)
     
596. Primer for identification of yeast (ITS or 18Sr RNA gene) - (reply: 3)
     
597. How to best store PCR product? - (reply: 2)
     
598. RT-PCR primer design - (reply: 1)
     
599. After PCR, One Band on 1.2% Agarose but 2 bands on 8M Urea, 8% PAGE - (reply: 1)
     
600. Odd gel run following PCR - (reply: 28)
     
601. Qiagen PCR Array Reagents? - (reply: 1)
     
602. No or very weak band using 16s primers - (reply: 1)
     
603. No PCR amplification with b-actin primers - (reply: 1)
     
604. Is PCR efficiency higher if mutation is in the middle of primer? - (reply: 7)
     
605. Site-directed, PCR works but no colonies - (reply: 2)
     
606. RUNX3 Methylation specific PCR not working-please help - (reply: 8)
     
607. Primer Design Help: GFP primers for Arabidopsis - (reply: 1)
     
608. No PCR amplified with long primers - (reply: 10)
     
609. PCR optimization: PCR vs qPCR - (reply: 4)
     
610. should I do blunt end my pcr product before ligation - (reply: 2)
     
611. PCR smear for genomic samples - (reply: 4)
     
612. Smaller band appear under Main product with every primers sets from PCR - (reply: 3)
     
613. another primer dilution question - (reply: 2)
     
614. i need help with virus pcr - (reply: 2)
     
615. Primer Efficiency - (reply: 2)
     
616. Experimental set up for qRT PCR - (reply: 13)
     
617. A faster way to pick single colony clones for PCR screening? - (reply: 3)
     
618. Colony PCR works but DNA yeild very low after miniprep - (reply: 2)
     
619. How should I optimize my PCR - (reply: 2)
     
620. Second round of PCR after gel extraction fails miserably - (reply: 3)
     
621. an effective way to do a yeast colony pcr - (reply: 2)
     
622. Conversion of ng/ul of DNA to ng for a PCR reaction - (reply: 2)
     
623. urgent help plz with RT-PCR - (reply: 3)
     
624. oligo(dt) 15 vs random primers - (reply: 3)
     
625. Help to optain a long PCR produc - (reply: 3)
     
626. Multiple bands in eluted PCR product - (reply: 1)
     
627. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 5)
     
628. Problems with two step RT-PCR - (reply: 2)
     
629. Getting genomic DNA for PCR - (reply: 4)
     
630. primer dilutions - (reply: 4)
     
631. Genotyping PCR primers - (reply: 1)
     
632. Conventional PCR - (reply: 1)
     
633. PCR of entire plasmid followed by self-ligation for mutagenesis- what issues mig - (reply: 2)
     
634. PCR product loss on cleanup - (reply: 2)
     
635. MSP Primers not working - (reply: 6)
     
636. Primers too dilute - (reply: 1)
     
637. Amplification curves jagged - (reply: 1)
     
638. Cloning HELP- Possible Primer issues - (reply: 6)
     
639. No PCR product in site-directed mutagenesis - (reply: 4)
     
640. Giardia/Crypto real time PCR late amplification - (reply: 1)
     
641. How to dilute the template DNA for PCR - (reply: 7)
     
642. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
     
643. Need help for PCR - (reply: 5)
     
644. PCR primer pairs - university exam - (reply: 7)
     
645. unespecific band PCR from mouse genomic DNA - (reply: 9)
     
646. Help with primer design through PRIMER3 - (reply: 4)
     
647. Homozygous Heterozygous by PCR/Southern blot - (reply: 3)
     
648. Normalizing to negative control primers for ChIP qPCR - (reply: 3)
     
649. Plasmid vs cDNA amplification by PCR - (reply: 2)
     
650. Tm of primers - prediction and verification - (reply: 1)
     
651. PCR cloning...help! - (reply: 5)
     
652. RT-PCR contamination in negative control. - (reply: 1)
     
653. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
     
654. how to design primers for 16sr RNA - (reply: 24)
     
655. how to do RT-PCR for16srRNA of a certain bacteria? - (reply: 4)
     
656. Help with primer design - (reply: 3)
     
657. Primer Tm calculation. - (reply: 1)
     
658. low concentration of purified PCR product - (reply: 2)
     
659. PCR smearing and...problems! - (reply: 7)
     
660. Running Primers on Agarose Gel - (reply: 6)
     
661. PCR product now not getting with same primers and other PCR conditions - (reply: 4)
     
662. universal primers for PCR ribotyping - (reply: 6)
     
663. will the template of PCR be added A ? - (reply: 2)
     
664. Colony PCR not working - (reply: 4)
     
665. Primers for Introduction of new restriction sites to a vector - (reply: 3)
     
666. Primer design: Tm above or below Ta? - (reply: 5)
     
667. Oligo primers for shRNA construction - (reply: 1)
     
668. Design primers for expression cloning - (reply: 11)
     
669. Designing a synthetic gene to serve as the positive control for two separate PCR - (reply: 5)
     
670. primer dilution query - (reply: 2)
     
671. Real Time PCR polymerase enzyme - (reply: 3)
     
672. Estimating size of PCR products in a agarose gel - (reply: 4)
     
673. Sequencing of PCR product - (reply: 4)
     
674. PCR contamination problem - (reply: 2)
     
675. Using BLAST to check primers - (reply: 1)
     
676. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
     
677. What is the function of a third primer in a PCR mix?? - (reply: 2)
     
678. How can we design a primer ? step by step method - (reply: 4)
     
679. cDNA synthesis or RT-PCR - (reply: 3)
     
680. PCR on cDNA and SGamplification??? - (reply: 1)
     
681. EPIGENETICS-NEWBIE-Weird/too close primers for pyrosequencing assay - (reply: 2)
     
682. real time PCR protocol - controls? - (reply: 2)
     
683. Long primers (60bp) and 5kb PCR product problems - (reply: 5)
     
684. Abnormal plot of real time one-step RT-PCR, how to solve! - (reply: 1)
     
685. SOS: new real time PCR machine needed.... which one? - (reply: 5)
     
686. which step in the pcr cleanup process could harm the cohesive ends - (reply: 1)
     
687. My product of PCR haven't the lenght right after purification....... What - (reply: 2)
     
688. Is it possible to amplify cDNA with hexamer primers or oligo dT primers to have - (reply: 1)
     
689. PCR and UV spectrophotometry - (reply: 3)
     
690. RT-PCR in a single tube - (reply: 3)
     
691. why Phusion polymerase is not recommended for overlap PCR? - (reply: 1)
     
692. annealing temperature for 3 primer pairs? - (reply: 4)
     
693. PCR Reaction - (reply: 3)
     
694. Impact of primer sequence on TA cloning - (reply: 3)
     
695. problems with Bisulfite PCR - (reply: 1)
     
696. low pcr product - (reply: 3)
     
697. overlapping PCR protocol - (reply: 12)
     
698. PCR to amplify my insert not working - (reply: 6)
     
699. Problem with PCR amplification- high 3' stability - (reply: 3)
     
700. Home made real-time PCR problem - (reply: 4)
     
701. High RNase concentration interfering with PCR? - (reply: 8)
     
702. Pcr primers - (reply: 2)
     
703. PCR failure from yeast genomic DNA - (reply: 1)
     
704. failure of PCR from yeast genomic DNA - (reply: 1)
     
705. RT-Q PCR primer design question - (reply: 2)
     
706. high MW dimers - (reply: 2)
     
707. Strange RT-PCR amplification plots - (reply: 9)
     
708. How to clean extracted faecal DNA? (removing PCR inhibitors) - (reply: 1)
     
709. What products will I get from this pcr? - (reply: 1)
     
710. PCR buffer without Tris - (reply: 1)
     
711. PCR Water - (reply: 4)
     
712. multiplex rtqPCR and custom primer/probe design - (reply: 2)
     
713. Which Brand of real-time PCR machine - (reply: 6)
     
714. Problem with PCR - large extra bands - (reply: 3)
     
715. big differnece in my primers Tm - (reply: 2)
     
716. Designing primers for the ORFs - (reply: 9)
     
717. Two-Step PCR - (reply: 2)
     
718. Gradient PCR - (reply: 2)
     
719. PCR with phophorylated primers - (reply: 1)
     
720. Problem with New qPCR primers - help! - (reply: 6)
     
721. Designing primers for miRNA located on minus strand. - (reply: 2)
     
722. Plasmid sequencing without primers - (reply: 1)
     
723. Smallest insert in PCR - (reply: 6)
     
724. troubleshooting RT-PCR: possible nuclease issues? - (reply: 2)
     
725. loop-mediated amplification - (reply: 2)
     
726. Blunt end PCR product ligation - (reply: 1)
     
727. Help with high Ct values for Real-Time PCR - (reply: 1)
     
728. Genotyping by allele specific PCR - (reply: 6)
     
729. RT-PCR primers and the poly(A) tail - (reply: 2)
     
730. NO Insert found in the Colony PCR - (reply: 15)
     
731. No band in PCR - (reply: 5)
     
732. overlapping primer - (reply: 1)
     
733. PCR primers help - (reply: 5)
     
734. PCR smear after one-month storage - (reply: 2)
     
735. Primer dimers - (reply: 2)
     
736. RT-PCR inhibited after Turbo DNase treatment - (reply: 3)
     
737. Real-Time PCR using ABI Fast Sybr Green chemistry - (reply: 2)
     
738. Simple question of dNTP's for PCR - (reply: 7)
     
739. Need help for PCR for AT rich gene - (reply: 3)
     
740. How long I can keep my template added PCR reaction mix before running PCR? - (reply: 4)
     
741. Primer validation and PCR efficiency - (reply: 4)
     
742. How to calculate the price of PCR test per sample? - (reply: 6)
     
743. Contamination in negative control of PCR (No template control) - (reply: 6)
     
744. cloning with nested pcr - (reply: 3)
     
745. What is the maxium amount of glycerol can be added in a PCR reaction? - (reply: 3)
     
746. PCR is amplifying non-specific fragment - (reply: 6)
     
747. ChIP amplification problems - (reply: 1)
     
748. real time RT-PCR problems - (reply: 2)
     
749. should I design primers spanning both intron and exon - (reply: 2)
     
750. RT-PCR - (reply: 1)
     
751. RNA extraction from FFPE cancer tissues for real-time RT-PCR - (reply: 2)
     
752. Template DNA (plasmid) concentration for PCR - (reply: 4)
     
753. PCR with a very long and a short primer - (reply: 5)
     
754. Ligation of 16kb PCR product - (reply: 5)
     
755. Multiplex PCR does not work - (reply: 3)
     
756. Help: Linear Amplification Using Hydrolysis Probes - (reply: 3)
     
757. Problem with genomic PCR (2.4 kb) - (reply: 1)
     
758. Comparison between two primers pairs? Is it possible? - (reply: 3)
     
759. Rt-PCR primer design - (reply: 7)
     
760. Length of non-binding sequence in primers with RE site - (reply: 6)
     
761. ChIP-PCR: Amplification in IgG negative control - (reply: 1)
     
762. Getting a smaller PCR product than required - (reply: 6)
     
763. Difficult sequences in PCR - (reply: 2)
     
764. why is real time pcr curve like this? - (reply: 3)
     
765. PCR inconsistency - (reply: 2)
     
766. Orientation of primers - (reply: 2)
     
767. PCR, neg control & no. of cycles - (reply: 2)
     
768. Strange PCR result from genomic DNA - (reply: 3)
     
769. Bright bands of PCR products stick in gel wells - (reply: 1)
     
770. methylation analysis with MS-HRM (primer design) - (reply: 1)
     
771. whole plasmid pcr? - (reply: 6)
     
772. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
     
773. salts in PCR - (reply: 4)
     
774. Strange amplification plot - (reply: 4)
     
775. Conventional PCR of different dilutions - (reply: 1)
     
776. primer resuspension - (reply: 2)
     
777. Principles of overlap PCR - (reply: 7)
     
778. ligation of pcr product into expression vector - (reply: 4)
     
779. Unable to PCR!!!! - (reply: 3)
     
780. Help designing primers with restriction sites - (reply: 3)
     
781. Why primers shouldn't have Tm difference greater than 5°C? - (reply: 5)
     
782. Low A260/A280 ratio after gel purification using Promega PCR Clean-up System - (reply: 2)
     
783. Abnormal amplification curve - (reply: 1)
     
784. Problem with Semiquantitative PCR - (reply: 4)
     
785. Advice on optimising bisulfite PCR sought - (reply: 2)
     
786. Help: I am loosing the DNA during PCR purification - (reply: 7)
     
787. PCR primer with no extra DNA end - (reply: 5)
     
788. PCR colonies of Pichia pastoris - (reply: 1)
     
789. ROX reference dye for quantitative RT-PCR - (reply: 1)
     
790. PCR problem - (reply: 9)
     
791. why PCR product smeared? - (reply: 2)
     
792. Tm calculation for primers with RE sites and overhangs - (reply: 4)
     
793. silincing box, no amplification - (reply: 3)
     
794. Comparative semiquantitative RT-PCR - (reply: 2)
     
795. Bands in my PCR Controls - (reply: 3)
     
796. cloning 2 PCR products into pGEM-T easy vector - (reply: 1)
     
797. Strange band in colony PCR - (reply: 2)
     
798. In a pcr why doesnt the DNA reneal during annealing process ? - (reply: 2)
     
799. Multiplex PCR - (reply: 12)
     
800. Real time pcr primers JunB, Foxh1, Klf4, PRMT1, PRMT4 and PRMT5 needed! - (reply: 2)
     
801. PYO pcr stopped working. - (reply: 1)
     
802. Bands seen with qPCR missing in regular PCR - (reply: 5)
     
803. checking msp primers - (reply: 2)
     
804. PCR product one day, none the next day - (reply: 4)
     
805. PCR on colony - (reply: 10)
     
806. PCR reaction ISSUES???? need help - (reply: 4)
     
807. PCR using CDNA as template - (reply: 2)
     
808. RT-PCR doesn't work with all RNA used - (reply: 1)
     
809. Need help amplifying repeating sequence. - (reply: 5)
     
810. Real Time PCR 101...help! - (reply: 1)
     
811. RT-PCR primer design for full length cDNA cloning - (reply: 6)
     
812. problems with PCR confirmation of insert . HELP ! - (reply: 1)
     
813. Another primer dimers problem - (reply: 23)
     
814. Colony PCR Problem!!!! - (reply: 5)
     
815. using PCR product as standard template - (reply: 3)
     
816. PCR kit - (reply: 7)
     
817. Make additional cycles with an already finished pcr product - (reply: 3)
     
818. Amplification in water control but not in samples - (reply: 3)
     
819. PCR products form with DNA, but not with c-DNA - (reply: 2)
     
820. question about pcr amplification efficiency? - (reply: 1)
     
821. DNA polymerase: recombinant or native? - (reply: 3)
     
822. pcr clean-up fail? - (reply: 1)
     
823. MSP U primers very difficult to amplify... - (reply: 4)
     
824. No gene amplification, 18s amplification is fine - (reply: 1)
     
825. ChIP-qPCR gives odd amplification plot - (reply: 5)
     
826. RT-PCR stoped working!!! Same samples, same primers, same enzyme! - (reply: 3)
     
827. sequential cloning of multiple PCR products - how to do? (reply: 3)
     
828. SYBR QPCR problem, please help! strange melting curve... - Primer dimers? Or other problems? (reply: 4)
     
829. Primer design - (reply: 3)
     
830. Tomato Actin PCR+Images included - (reply: 2)
     
831. single primer PCR - (reply: 5)
     
832. Direct sequencing PCR product - for bisulfite sequencing (reply: 3)
     
833. cDNA synthesis + amplification - (reply: 2)
     
834. Design strategy for real-time RT-PCR, how to decide on what gene of interest and - Newbie that's completly mRNA confused, please help! (reply: 2)
     
835. LacZ PCR Problems - (reply: 2)
     
836. cells stably expressing T7 RNA polymerase - (reply: 1)
     
837. Primer concentration - (reply: 4)
     
838. nested PCR with low target DNA? - (reply: 2)
     
839. RT-PCR calibrator - (reply: 2)
     
840. RNA - extraction and pcr (reply: 1)
     
841. problem in pcr - (reply: 2)
     
842. Can someone recommend me a virtual PCR software? - (reply: 6)
     
843. Trouble with PCR and electroporation - Trying to make mutants via PCR (reply: 4)
     
844. Primer 3' mismatch - Strange experience (reply: 9)
     
845. real-time PCR after restriction enzyme digestion - (reply: 6)
     
846. wrong plot amplification in one-step RT- PCR - one step real time RT PCR (reply: 1)
     
847. Adding loading/tracking dye to PCR mix? - (reply: 10)
     
848. PCR: Band in one lane, streaking in the other..HELP! - (reply: 3)
     
849. DNA polymerase with single G 3'-end overhang activity - (reply: 3)
     
850. Primer BLAST and NetPrimer vs Primer3Plus - (reply: 3)
     
851. relative expression of genes using semiquantitative PCR - Semi-quantitative PCR (reply: 1)
     
852. how to get rid of non specific bands in PCR - (reply: 1)
     
853. PRIMER DILUTION - STOCK SOLUTION TO A WORKING STANDARD (reply: 2)
     
854. PCR tubes mixer & spinner ( one device ) - recommendations ? (reply: 2)
     
855. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
     
856. PCR cloning problem - could not get colony (reply: 4)
     
857. pcr contamination - (reply: 4)
     
858. Are my MSP primers good? - MSP primers design by Methyl Primer Express v1.0 (reply: 2)
     
859. Large Non-specfic bands in PCR - (reply: 3)
     
860. PCR clonning - (reply: 3)
     
861. Difference between PCR/cloning DNA in plasmids - (reply: 2)
     
862. 2 bands in blue colony PCR - (reply: 5)
     
863. Primer dilution --> problem.. - (reply: 16)
     
864. Help! Smear in Real-Time PCR Product - (reply: 1)
     
865. alternative to Tris buffer in strand displacement (BST polymerase) - looking into alternatives to the NEB ThermoPol buffer (reply: 2)
     
866. Sybr green RT-qPCR primer - (reply: 1)
     
867. Sybr green RT-qPCR primer - (reply: 1)
     
868. primer design - microalgae (reply: 2)
     
869. Designing primers - What is more important Ta or primer's Tm - (reply: 3)
     
870. Please help! no PCR bands+Description of my PCR protocol provided :( - (reply: 8)
     
871. real time rt PCR - bloodyurine - inhibition - (reply: 1)
     
872. PCR yield in ng/ul - (reply: 1)
     
873. No Amplification in PCR - (reply: 4)
     
874. Real time PCR and percentage loss - (reply: 1)
     
875. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 3)
     
876. Problem with smear in PCR with certain templates - (reply: 4)
     
877. Real time PCR in the presence of heme - (reply: 3)
     
878. issues in PCR amplification - (reply: 2)
     
879. Problems with PCR in general - (reply: 1)
     
880. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 2)
     
881. how to overcome primer contamination - need protocol (reply: 11)
     
882. RT-PCR primer design - Primer design and evaluation (reply: 7)
     
883. CHIP analysis by PCR - (reply: 4)
     
884. STANDARDISATION OF MULTIPLEX PCR - IN order to standardise a multiplex PCR for 3 set of primers (reply: 3)
     
885. Primer,probes dilution - (reply: 3)
     
886. Splice varient quantification in RT real time PCR? - (reply: 1)
     
887. negative control for msp pcr - (reply: 2)
     
888. Calculating geometric mean for real-time PCR - (reply: 5)
     
889. template transfer? - how does the rna polymerase switches template? (reply: 1)
     
890. PCR Gel nonspecific band - (reply: 13)
     
891. semiquantitative pcr - (reply: 1)
     
892. Sudden problems with cDNA PCR using Phusion Polymerase - (reply: 9)
     
893. PCR: Cloning Primers - (reply: 8)
     
894. PCR product - (reply: 5)
     
895. pcr amplify an insert in plasmid? - (reply: 2)
     
896. Sybr Green Real Time PCR - Amplification plot - Any problem? (reply: 1)
     
897. Exon Spanning PCR Primers - (reply: 1)
     
898. Colony PCR - (reply: 1)
     
899. PCR to confirm double crossover - (reply: 1)
     
900. Primer sequences - (reply: 1)
     
901. Why does freezing/thawing DNA samples first improve PCR results? - (reply: 8)
     
902. smears in PCR products (bisfulite treated DNA) - (reply: 4)
     
903. PCR Primers - (reply: 2)
     
904. Cleaning up RT-PCR product before sequencing - (reply: 1)
     
905. Template Amount for PCR Amplification - (reply: 6)
     
906. How to blast methylation specific and unspecific primers - (reply: 1)
     
907. RT-PCR cDNA synthesis - (reply: 6)
     
908. problems amplifying from cDNA - (reply: 4)
     
909. Real-time PCR about Incorrect setup of the data collection stage - (reply: 1)
     
910. No DNA after PCR purification with QiaQuick? - (reply: 4)
     
911. restriction digestion against colony PCR - (reply: 1)
     
912. How to check primers are of correct sequence or not? - (reply: 2)
     
913. Primers... - (reply: 1)
     
914. When are primer dimers a problem? - (reply: 1)
     
915. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
     
916. PCR: always got band in the negative control - (reply: 8)
     
917. primer with higher melting temperature - (reply: 2)
     
918. Is pyrosequencing necessary? - Sequencing PCR products (reply: 2)
     
919. Problems designing primers for BSP - (reply: 2)
     
920. qPCR primer design - possible off-target (reply: 1)
     
921. Primer dimer formation and real-time PCR - (reply: 7)
     
922. Identify PCR products - (reply: 3)
     
923. PCR genomic DNA using cell lysate - (reply: 1)
     
924. Maximum size of overhangs in PCR - (reply: 6)
     
925. Colony PCR doesn't work anymore - (reply: 5)
     
926. RT-PCR t-test, and ANOVA - (reply: 1)
     
927. Primer Design Urgent! - (reply: 1)
     
928. PCR basics - I'm new to all things lab and need some help! (reply: 3)
     
929. Able to see correct product with 2 rounds of PCR, but my PI doesn't want me - How do I convince her that "2 rounds of PCR is OKAY?" (reply: 1)
     
930. PCR of microRNA first strand cDNA - (reply: 1)
     
931. how to know my primer's sense ??? - help ! (reply: 2)
     
932. smear in pcr +DNA polymerase mixture for long PCR - (reply: 10)
     
933. High Ct value in Real Time RT PCR for NTC - Real Time RT PCR troubleshooting (reply: 5)
     
934. problems with MS-HRM - late detection of PCR signal - high resolution melting, methylation (reply: 2)
     
935. intron spanning primers for non model organisms - (reply: 2)
     
936. Primer design - (reply: 2)
     
937. Troubleshoot PCR, Product band missing, internal control is present - (reply: 1)
     
938. qPCR amplification interference fixed with freezing - (reply: 6)
     
939. Suggest me a mastermix for conventinal PCR... - (reply: 11)
     
940. software for quantifing PCR product band - (reply: 1)
     
941. Multiple bands from purified PCR product - (reply: 3)
     
942. Sybr Green Real Time PCR - Melt curve - Any problem on the melt curve? (reply: 3)
     
943. Doing PCR on Nebulized DNA - (reply: 2)
     
944. Direct PCR Sequencing of BS Products - Having trouble (reply: 1)
     
945. RT-PCR - (reply: 9)
     
946. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
     
947. inverse pcr - (reply: 2)
     
948. Will I need interplate calibration for my RT-PCR experiment? - (reply: 3)
     
949. Primer seq - (reply: 2)
     
950. Urgent: should you dry PCR product - (reply: 13)
     
951. PCR product size - (reply: 6)
     
952. amplification plot raises to early - What is the explanation for this problem? (reply: 1)
     
953. oligo dt and random primer - (reply: 3)
     
954. mRNA Search for RT-PCR (U to T) - (reply: 2)
     
955. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
     
956. PCR Mastermix - (reply: 4)
     
957. PCR triplicates versus one reaction - differences? (reply: 17)
     
958. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
     
959. Do low binding 0.2ml PCR tubes exist? - (reply: 3)
     
960. Primers and self and hetero dimers - (reply: 1)
     
961. Really weird amplification curves - (reply: 1)
     
962. Primers for qPCR - (reply: 3)
     
963. Placing dNTP in 64 C waterbath - Would this ruin the solution? (reply: 3)
     
964. RT-PCR primer - (reply: 3)
     
965. PCR problem - basic PCR for plasmid amplification (reply: 2)
     
966. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
     
967. mutation using overlapping PCR, what's the success rate? - (reply: 3)
     
968. Unpredictablity of PCR product - (reply: 5)
     
969. total cDNA amplification by PCR - (reply: 2)
     
970. Detection limit of a conventional PCR - (reply: 2)
     
971. PCR for MULTIPLE mutagenesis - (reply: 4)
     
972. Using Sybr green in realtime pcr - (reply: 1)
     
973. IF ITS1 and ITS4 using as primer and working on the fungi . Shall we get single - (reply: 12)
     
974. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
     
975. Dissolved Primer pellet in pure ethanol instead of water - (reply: 1)
     
976. Dissolved Primer pellet in pure ethanol instead of water - (reply: 1)
     
977. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
     
978. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
     
979. Dissolved Primer pellet in pure ethanol instead of water - (reply: 4)
     
980. Digested PCR product migrate slower than uncut - (reply: 8)
     
981. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
     
982. PCR question - (reply: 2)
     
983. positive colony PCR, negative restriction digest, positive PC from minipreps - Cloning nightmare (reply: 2)
     
984. 2 rounds PCR got problem - (reply: 2)
     
985. PCR help minus strand - Primer design (reply: 1)
     
986. chloramphenicol storage and amplification - (reply: 3)
     
987. Smear in long distance PCR - (reply: 39)
     
988. TP-PCR or three primer PCR - TP-PCR or three primer PCR (reply: 9)
     
989. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
     
990. TOUCH DOWN PCR - (reply: 2)
     
991. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
     
992. PCR stopped working - After changing buffer (reply: 5)
     
993. ChIP PCR question - (reply: 33)
     
994. PCR protocol questions! - (reply: 2)
     
995. TOUCH DOWN PCR NEEDS EXPLANATION - (reply: 3)
     
996. DNA amount calculation for PCR - (reply: 14)
     
997. ChIP primer design - (reply: 1)
     
998. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
     
999. Bisulfite sequencing primer design query (probably simple) - (reply: 4)
     
1000. Using digoxigenin in PCR? - Want to create a FISH probe (reply: 2)
      
1001. How to analyze ChIP PCR data - (reply: 10)
      
1002. Questions regarding RT-PCR optimization - (reply: 2)
      
1003. number of copies after pcr? - (reply: 1)
      
1004. PCR double-bands - (reply: 1)
      
1005. Problems with semi-quatitative PCR - (reply: 1)
      
1006. Question about Primers - (reply: 3)
      
1007. Odd question. Can I use DNase to decontaminate Primers? - Primer decontamination (reply: 2)
      
1008. Large fragment amplification failed - (reply: 3)
      
1009. purification of PCR product for cloning in a vector - (reply: 3)
      
1010. elimination of Protein interference from DNA - for RT-PCR (reply: 2)
      
1011. PCR reaction calculation - (reply: 2)
      
1012. designing MSP primers (dimers NOOOOOOOO!) - (reply: 1)
      
1013. Primer design with a tag - (reply: 4)
      
1014. no increase in fluorescence in my real time PCR - (reply: 3)
      
1015. PCR Problem: Inconsistency with the results since one week - Inconsistency (reply: 1)
      
1016. Help with real-time PCR quantification of miRNA - (reply: 8)
      
1017. PCR, using gel extracted band as a template - (reply: 4)
      
1018. PCR Amplification: Tips on simple things to keep an eye out for - (reply: 1)
      
1019. Primer3 and Blast - how can I search for primers against a local database. (reply: 3)
      
1020. PCR for sequencing genomic DNA (multiple alleles) - Are there preferences for amplification of certain sequences? (reply: 1)
      
1021. Combining forward and reverse primer gives different size on gel - (reply: 2)
      
1022. Q-PCR and RT-PCR - (reply: 3)
      
1023. calculating total amount - in a pcr reaction (reply: 4)
      
1024. Problems with amplifying microRNA - (reply: 3)
      
1025. PCR of complement genomic DNA - (reply: 3)
      
1026. primers for fungal identification - (reply: 9)
      
1027. confusing PCR - (reply: 1)
      
1028. Strange problem with digestion and dephosporylation of PCR product - (reply: 2)
      
1029. PCR for long and repetitive region from genomic DNA - (reply: 4)
      
1030. difference between PCR primers and sequencing primers - (reply: 1)
      
1031. How do I PCR a DNA fragment with >200 CGG repeats? - (reply: 2)
      
1032. PCR of repeated region - (reply: 1)
      
1033. Sense and antisense DNA, and primer design - (reply: 6)
      
1034. PCR bands in NTC but NOT in negative samples - (reply: 2)
      
1035. housekeeping genes in PCR - (reply: 1)
      
1036. Forward primer not working during DNA sequencing - DNA sequencing (reply: 10)
      
1037. Question about RealTime PCR Primer Design - (reply: 1)
      
1038. Designing a Primer with a RS that has a W - (reply: 1)
      
1039. Primers stopped working!? - PCR Primers (reply: 6)
      
1040. NFK beta primers - (reply: 4)
      
1041. final PCR product - need clarifications (reply: 1)
      
1042. Mutagenesis PCR problem - Help with PCR mutagenesis (reply: 5)
      
1043. Is DNase necessary for primers designed on the exon-exon boundary?? - (reply: 2)
      
1044. Unable to get proper PCR amplification - PCR does not correlate protein expression (reply: 3)
      
1045. RT-PCR result !! - (reply: 4)
      
1046. mirna overexpression/ library versus single construct/ unspecific pcr - unspecific band when pcr on gDNA from library but not on gDNA fron sin (reply: 2)
      
1047. Troubleshooting help: Why do my amplification curves look like this? - (reply: 8)
      
1048. Agarose gel electrophoreses for PCR products ? - (reply: 4)
      
1049. why genomic can't be use for pcr? - (reply: 2)
      
1050. Primer design - Primer design (reply: 2)
      
1051. Re-use unamplified PCR product - (reply: 2)
      
1052. Reason for odd PCR conditions - (reply: 3)
      
1053. Amplification of region from sperm RNA - sperm RNA stability (reply: 4)
      
1054. Amplification of 3.4 kb product from RNA for cloning - (reply: 5)
      
1055. pcr - problem in pcr (reply: 2)
      
1056. Primers for PCR - Forward and Reverse Primer Sequences (reply: 4)
      
1057. How to present and analyze these real-time PCR data? - (reply: 2)
      
1058. Deoxyribosenucleoside vs. Deoxyribosenucleotide - What does dNTP stand for? (reply: 2)
      
1059. RT-PCR: DNA contamination, one vs two step - RNA work (reply: 11)
      
1060. PCR Master mix - (reply: 2)
      
1061. cDNA storage and real time RT PCR - (reply: 3)
      
1062. Real Time PCR Primers and Probes - (reply: 2)
      
1063. Anyone make their own PCR cloning vector? - (reply: 5)
      
1064. Stability of PCR T-overhangs - (reply: 1)
      
1065. Loss of bands at higher Tm in presence of DMSO... - Attempt to optimise DMSO methylation-spec PCR (reply: 4)
      
1066. PCR product wrong size! Need help! - (reply: 9)
      
1067. pooling different pcr cycles? - (reply: 1)
      
1068. Amplification of concatenated linear ligated fragments - (reply: 4)
      
1069. Clinical Real-time PCR assay - (reply: 1)
      
1070. Need help - How we can design primer for miRNA (reply: 1)
      
1071. primer and promoter sequences - (reply: 1)
      
1072. Standard curve for real-time PCR - (reply: 2)
      
1073. designing of primers - (reply: 2)
      
1074. Primer Tm differences/ PCR - (reply: 1)
      
1075. Primer design to screen silenced lines - Primer design tips to screen silenced lines (reply: 3)
      
1076. single gene amplification - cDNA synthesis and amplification with gene specific primers (reply: 2)
      
1077. d2EGFP Primers for Genotyping - ...where to get the sequence? (reply: 5)
      
1078. BAC recombineering problem - BAC clones positive for double selection but negative for PCR, why? (reply: 1)
      
1079. Designing primer to remove his-tag - (reply: 1)
      
1080. PCR Primer Dilution from F+R - (reply: 1)
      
1081. primer reconstitution - primer reconstitution (reply: 4)
      
1082. Not-Quite-Nested PCR - (reply: 2)
      
1083. PCR product too short - (reply: 6)
      
1084. Anyone know the margin of error for PCR amplicon size visualized on an agarose g - Margin of Error (reply: 2)
      
1085. Primer design for insert amplification - Is this correct? (reply: 2)
      
1086. got smear on the PCR gel !!! - (reply: 4)
      
1087. need to know minimum amount of template DNA needed for pcr amplification - pcr (reply: 2)
      
1088. "Unzipped" PCR band - (reply: 3)
      
1089. Review on new amplification techniques - (reply: 2)
      
1090. What's the highest DNA concentration for PCR? - before being inhibited? (reply: 7)
      
1091. DOT BLOT for pcr product - No result obtain (reply: 1)
      
1092. melting temperature in real time pcr - melting temperature in real time pcr (reply: 5)
      
1093. Amplification of bisulfite converted gDNA for sequencing - (reply: 1)
      
1094. HRM primers - (reply: 1)
      
1095. PCR amplification with Pfu / quality of DNA - (reply: 4)
      
1096. primer design - (reply: 2)
      
1097. Is it possible to compare data between real-time PCR plates - (reply: 1)
      
1098. Conventional PCR problems - wisamni2004 (reply: 2)
      
1099. New to sequencing, primer design - (reply: 2)
      
1100. Is it possible to know the live bacteria using PCR for a certain genus? - (reply: 5)
      
1101. Is it possible to know only the live bacteria using PCR for a certain genus? - (reply: 1)
      
1102. incorrect/smaller band size for real time PCR - (reply: 1)
      
1103. Quickchange mutagenesis primer - (reply: 3)
      
1104. pcr / primer theory in bisulfite sequencing - (reply: 4)
      
1105. homologous amplicon for real time pcr - (reply: 1)
      
1106. Primer Design..need very basic advice. - Please help, I cant figure this out for the life of me. (reply: 2)
      
1107. How about Exiqon miRCURY miRNA PCR system? - (reply: 2)
      
1108. assistance with PCR amplification please - (reply: 3)
      
1109. I need an urgent help with q-pcr amplification plots - (reply: 11)
      
1110. Problems with the Specificity of the Primers - (reply: 2)
      
1111. confusion in real time PCR primers - Confusion in Real time pcr primers (reply: 3)
      
1112. Colony PCR with FALSE Positive Results ?? - (reply: 4)
      
1113. Degenerate primers PCR problem, Please help! - (reply: 2)
      
1114. Hotstart PCR and unspecific Amplification - (reply: 3)
      
1115. Primer design help needed - (reply: 1)
      
1116. Primers no longer work - (reply: 1)
      
1117. Sequencing - why are left-over PCR primers an issue? - (reply: 3)
      
1118. Primer Design with Primer-Blast - My experience has been worse than just using Primer3 and BLAT separate (reply: 4)
      
1119. RT PCR for known snps detection? - SNPs detection (reply: 1)
      
1120. colony PCR - (reply: 2)
      
1121. Calculating Primer concentrations for PCR - Is there an easy way to do this...help (reply: 3)
      
1122. primer Tm is too high, how tu get pcr product - help! (reply: 21)
      
1123. PCR reaction without template gives a product - (reply: 6)
      
1124. PCR wrong product size - (reply: 6)
      
1125. Wrong pcr product size - (reply: 4)
      
1126. real time PCR machine - (reply: 5)
      
1127. PCR very faint product - (reply: 1)
      
1128. Question about principle of PCR - (reply: 3)
      
1129. PCR amplification before bisulfite conversion - (reply: 6)
      
1130. 4 question for real time PCR - (reply: 5)
      
1131. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
      
1132. PCR didn't work - suspect the enzyme (reply: 2)
      
1133. Find universal primers in vector - any online tool? (reply: 6)
      
1134. Picking primers to confirm Illumina meth27 results - (reply: 2)
      
1135. Colony PCR Specificity - (reply: 2)
      
1136. Colony Pcr - primers - (reply: 5)
      
1137. iCODEHOP help: how are the reverse primers supposed to be read? - Degenerate primer design (reply: 1)
      
1138. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
      
1139. Primer Dilution Problem - D'oh! (reply: 2)
      
1140. Using PCR to create overhangs - (reply: 3)
      
1141. Creating overhangs with PCR - (reply: 4)
      
1142. Problem with PCR on bacmids - (reply: 1)
      
1143. Is there any software that can be sued to design degenerate primers - (reply: 1)
      
1144. Running real time pcr product on gel?.. - (reply: 4)
      
1145. Primer Decontamination - (reply: 2)
      
1146. PCR mutagenesis of plasmid - (reply: 5)
      
1147. tools for checking non-specific binding of primers -
      (reply: 1)
      
1148. Primer designed for ARMS PCR - (reply: 2)
      
1149. Problem with Telomeric Length measurement using RT-PCR - (reply: 1)
      
1150. methylation specific PCR - Primers - (reply: 1)
      
1151. bacterial identification using real time PCR - (reply: 1)
      
1152. Beta actin Primer designing - Will anyone plz send me the sequence of primers for rat beta-actin (reply: 2)
      
1153. real time PCR inhibition control - a test for the presence of PCR inhibitors (reply: 3)
      
1154. PCR analysis method- delta or delta delta? - RNA, qRT PCR, delta Ct method (reply: 4)
      
1155. colony PCR for subcloning gene - (reply: 1)
      
1156. Problems with Multiplex PCR - Problems with Multiplex PCR (reply: 4)
      
1157. Using the same PCR plate in more runs - Just curious about it. (reply: 1)
      
1158. PCR reamplification - (reply: 2)
      
1159. Need advice on a smear from primer-primer binding PCR reaction. - (reply: 2)
      
1160. How many colonies to screen (colony PCR)? - (reply: 4)
      
1161. Fusion PCR, bright smear from well to end(with very weak or no band) - (reply: 1)
      
1162. Primer Design - aligning sequences for probe/primer design (reply: 2)
      
1163. question about pcr - (reply: 3)
      
1164. RT-PCR Taqman no change in gene expression - GATA3 gene expression in splenocytes, a tough one (reply: 3)
      
1165. How to construct a standard curve for real time PCR - (reply: 1)
      
1166. Whole Genome Amplification of bisulfite treated DNA - (reply: 7)
      
1167. PCR with one primer - (reply: 1)
      
1168. colony PCR inconsistent - (reply: 4)
      
1169. PCR contamination - (reply: 19)
      
1170. PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
      
1171. If a 20bp primer differs from template DNA by 20bp, can PCR work? - (reply: 1)
      
1172. can you please help me to design primers for reverse transcription of miRNA and - miRNA primers (reply: 2)
      
1173. PCR not working - No amplification - (reply: 5)
      
1174. Quantification of PCR products - (reply: 1)
      
1175. fermentas clonejet pcr cloning kit - want opinion (reply: 1)
      
1176. Stratagenes Dpn1 cleaves my PCR product - (reply: 1)
      
1177. qPCR Amplification Efficiency too high - Efficiency of 115 - 145% (reply: 1)
      
1178. Help... my PCR don't come any more - PCR amplification desapeard (reply: 3)
      
1179. Nested PCR question - (reply: 1)
      
1180. High Tm on primers - cannot get a product - (reply: 8)
      
1181. Help: Very odd amplification graphs (RTPCR) - (reply: 3)
      
1182. undesired products in multiplex PCR - (reply: 3)
      
1183. NCBI primer blast problem - (reply: 1)
      
1184. Help with Multiplex Nested PCR - (reply: 2)
      
1185. Taq polymerase works but Phusion polymerase doesn't - How to achieve high-fidelity with robust amplification? (reply: 3)
      
1186. Question of q-pcr primer design - Tm difference from IDTDNA (reply: 2)
      
1187. primer sequence problem - (reply: 2)
      
1188. Finding bisulfite PCR primer sequence - (reply: 3)
      
1189. PCR -No band formation - (reply: 1)
      
1190. PCR - No Band formation - (reply: 3)
      
1191. No amplification after bisulfite treatment - (reply: 4)
      
1192. PCR AMPLIFICATION - (reply: 1)
      
1193. Ligation of Blunt PCR Product - (reply: 1)
      
1194. real time pcr need advise - (reply: 2)
      
1195. Opinion about LUX primers /D-LUX assay - What say you? (reply: 5)
      
1196. PCR & Cloning - (reply: 6)
      
1197. Long PCR Primers? Advice for generating multiple adjacent mutations - Designing long degenerate primers for shotgun alanine scanning (reply: 3)
      
1198. use of housekeeping gene in RT PCR chIP - (reply: 3)
      
1199. DNA pooling for PCR - Saving money PCR (reply: 7)
      
1200. Real Time PCR Standard curves - How many are required??? (reply: 1)
      
1201. PCR need some help - (reply: 4)
      
1202. How to design primers to check my candidates in a ChiP assay - (reply: 4)
      
1203. Left polymerase out overnight - Will it still work? (reply: 1)
      
1204. Primer Reconstitution--does temperature matter? - (reply: 5)
      
1205. Primer Design MSP, BSP, MS-HRM - (reply: 3)
      
1206. Primers that do not align at regions with SNPs. Where to find SNPs? - (reply: 2)
      
1207. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
      
1208. Gradient PCR works, but single temperature does not! HELP!! - (reply: 5)
      
1209. Primer efficiency - How to determine primer efficiency correctly? (reply: 4)
      
1210. no PCR product from input... - (reply: 8)
      
1211. MSP - unwanted amplification - (reply: 5)
      
1212. Smears in Bisulfite seq. PCR - (reply: 3)
      
1213. Primer design for qPCR - (reply: 2)
      
1214. Primer design for qPCR - (reply: 1)
      
1215. DNA bisulphited Amplification - the amplification dont produce any band!! (reply: 3)
      
1216. Pipettors for Real Time PCR (urgent) - Any good one? Any good recommended brands? (reply: 5)
      
1217. Multiple bands after bisulfite PCR - (reply: 7)
      
1218. PCR genomic DNA of high GC content - (reply: 6)
      
1219. GAPDH primer design and efficiency problems (new to rt-pcr) - (reply: 4)
      
1220. primer design tips - (reply: 3)
      
1221. PCR with Biotin Incorporation - (reply: 2)
      
1222. pcr product - hello all (reply: 1)
      
1223. design pcr primer - (reply: 1)
      
1224. promoter cloning PCR problem - what was wrong for my PCR set-up (reply: 3)
      
1225. amplification of GroEL from Wolbachia! - amplification of GroEL from Wolbachia! (reply: 2)
      
1226. PCR with long and complex primers - (reply: 2)
      
1227. Phospholylation of primers - (reply: 1)
      
1228. apoptosis by PCR? - (reply: 12)
      
1229. Re-using PCR plates? - (reply: 2)
      
1230. Touchdown PCR issues - (reply: 3)
      
1231. LINE1 OFR2 Primer Design - (reply: 1)
      
1232. phosphorylation of primers using PNK - (reply: 1)
      
1233. RT-PCR contamination issue - (reply: 3)
      
1234. Primers for yeast (18S) - (reply: 1)
      
1235. How to reduce the bisulfite PCR bias? - (reply: 4)
      
1236. What's the longest overhanging primer seq. you have used? - (reply: 2)
      
1237. Primer Concentration to lower Ct - (reply: 1)
      
1238. Problems with Stratagene's site directed mutagenesis kit - Primers, PCR, Mutagenesis (reply: 3)
      
1239. Primer Concentration Help - (reply: 4)
      
1240. who got a manual of ABI7500 PCR instrument ? - how to operate this instument ? (reply: 4)
      
1241. Primer Degradation - What exactly happens? (reply: 1)
      
1242. Smearing on ategarose gel of real time pcr product??? - (reply: 1)
      
1243. PCR/DNA Extraction Problem - (reply: 5)
      
1244. Primers in 2 exons - (reply: 2)
      
1245. NTC appear in real time pcr - (reply: 6)
      
1246. Problems with designing a primer - (reply: 2)
      
1247. How to clean-up 96-well microplates? - to re-use 96-well plates for PCR and sequencing (reply: 4)
      
1248. PCR of AT-rich DNA - (reply: 1)
      
1249. T7 RNA polymerase transcription and translation. - The maximum number of nucleotides between T7 promoter and first ATG (reply: 2)
      
1250. Overlapping PCR - really need help ! - (reply: 5)
      
1251. Freezing PCR product??? - (reply: 1)
      
1252. problem with pcr cloning from mouse cDNA - (reply: 2)
      
1253. Probe or Primer info for bovine AQP-1 - (reply: 6)
      
1254. PCR with Platinum Taq - product yield issues - (reply: 3)
      
1255. real time PCR - (reply: 7)
      
1256. Different sized product for same BS primer! - (reply: 2)
      
1257. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
      
1258. How do you estimate PCR product size ? - How do you estimate PCR product size ? (reply: 3)
      
1259. PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
      
1260. Real Time PCR Normalization - What if I have no choice for housekeeping genes to normalize RT PCR (reply: 3)
      
1261. Differences between primers for real-time PCR and RT-PCR - (reply: 6)
      
1262. primers with restriction site - (reply: 1)
      
1263. PCR product appear two close band in my gel - (reply: 15)
      
1264. The Case of a Missing Band: PCR Issue - (reply: 4)
      
1265. PCR: Smear bands, no amplification, got it all.. - PCR Mutagenesis (reply: 5)
      
1266. cDNA as PCR template - (reply: 1)
      
1267. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
      
1268. cloning a PCR product. Taq pol or High Fidelity pol? - (reply: 6)
      
1269. paranormal qpcr amplification activity - melting curves (reply: 3)
      
1270. Can a primer bind to DNA in a 5'-3 parallel fashion? - Just wondering... (reply: 1)
      
1271. PCR template concentration - (reply: 6)
      
1272. Bands in PCR negative control - (reply: 3)
      
1273. PCR efficiency calculated by Linreg - (reply: 3)
      
1274. Primer for Unkown Protein PCR - How to design primer for unknown protein (reply: 2)
      
1275. primer design by generunner DG - (reply: 1)
      
1276. degenerate pcr - (reply: 1)
      
1277. BSP primers design, help please - (reply: 2)
      
1278. primers with fluoresent dye PCR cycle question - (reply: 4)
      
1279. Urgent! Sequencing a mixture of ssDNA fragments of the same gene - ssDNA different in size amplified from a single primer (reply: 2)
      
1280. Smears on PCR products - (reply: 4)
      
1281. Dumb Reverse Transcription PCR Question - (reply: 2)
      
1282. Reverse transcription PCR - (reply: 1)
      
1283. PCR/RT-PCR beads - (reply: 2)
      
1284. High Amplification Efficiency in Std. Curve - (reply: 5)
      
1285. Non specific products in Bisulphite pcr - (reply: 4)
      
1286. Run PCR amplifications in agarose gels - (reply: 3)
      
1287. Problems with validating primers and low expression genes - (reply: 3)
      
1288. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
      
1289. Bisulfite sequencing PCR not working - (reply: 5)
      
1290. [Help]universal tag - universal tag in multiplex PCR (reply: 4)
      
1291. Tips for visualizing very faint bands in agarose gels? - (The PCR ain't going to get better, so the gel must!) (reply: 15)
      
1292. intron/exon spanning primers - (reply: 1)
      
1293. can I use routine PCR to assess whether a gene is expressed or not? - (reply: 3)
      
1294. expression cloning in TOPO TA and pET vectors - Very high and unusual non specific amplification in colony PCR (reply: 1)
      
1295. PCR product for sequencing - sample storage?? (reply: 2)
      
1296. Absolute quantification using Real Time PCR - (reply: 2)
      
1297. PCR of AT rich gene - - I am having trouble amplifying an AT rich sequence (reply: 4)
      
1298. question about blunt ligating Taq-amplified PCR product - (reply: 2)
      
1299. RT-PCR Gel - Ladder looks terrible and product is fuzzy! (reply: 1)
      
1300. MSP primer troubles - (reply: 3)
      
1301. Measuring global methylation using real time PCR - (reply: 2)
      
1302. Designing Primers for multiple Isoforms - (reply: 1)
      
1303. RT-PCR standard curve dilutions - (reply: 2)
      
1304. Rt-PCR problem !with 2.5 kb gene - (reply: 3)
      
1305. real time pcr melt curve and primer efficiency problem - (reply: 3)
      
1306. Aaaah I want to die!!!! PCR won't work - Why do the extractions that amplified 2 weeks ago fail now? (reply: 13)
      
1307. how to design primer for my n-terminal sequence - hot to design primer for my protein (reply: 2)
      
1308. PCR product dimer issue - (reply: 24)
      
1309. PCR additives Formamide - Formamide which one? (reply: 4)
      
1310. PCR Master mix - (reply: 1)
      
1311. Addition of Restriction sites into PCR primers - (reply: 4)
      
1312. Polymerase Chain Reaction (PCR) - Anylyzing my PCR gel (reply: 6)
      
1313. failure PCR amplification from low GC content gene - (reply: 5)
      
1314. How is this idea sounds to you? - A new approach for rapid sample preparation for PCR (reply: 1)
      
1315. conventional v real-time PCR applications - (reply: 1)
      
1316. Primer Design in 3´non translated version vs. coding region - (reply: 2)
      
1317. PCR reagents, can I use reagents from different manufacturers? - (reply: 6)
      
1318. different PCR primers for real-time and classic PCR - (reply: 3)
      
1319. primers deconamination??? - (reply: 6)
      
1320. PCR band too thick - PCR troubleshooting question (reply: 5)
      
1321. RT-PCR problem - (reply: 1)
      
1322. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
      
1323. primer check!!! - (reply: 6)
      
1324. PCR bands on gel electrophoresis - (reply: 5)
      
1325. Qiagen Qiaquick vs MinElute PCR purification kit for ChIP DNA - (reply: 10)
      
1326. long range PCR - (reply: 2)
      
1327. Annealing Temperature of biotinylated primers - (reply: 2)
      
1328. RT-PCR - New to RT-PCR info (reply: 2)
      
1329. PCR punch - (reply: 4)
      
1330. good amplification in classic PCR, no amplification in qPCR - (reply: 6)
      
1331. PCR off plasmid for screening - (reply: 2)
      
1332. PCR failed no product.. help - (reply: 10)
      
1333. number of molecules in PCR - (reply: 3)
      
1334. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
      
1335. primer for bisulfite sequencing in a known unmethylated region - is it safe? - (reply: 2)
      
1336. Primer clean-up - ExoSAP-It isn't working (reply: 6)
      
1337. Primer dilution - PCR primer dilution (reply: 2)
      
1338. Data acquisition for qPCR - Endpoint of annealing or endpoint of amplification? (reply: 3)
      
1339. Can't re-PCR the PCR product - (reply: 5)
      
1340. PCR reagent autoclave - (reply: 2)
      
1341. Bad PCR, is it due to conditions, reagents, or lack of DNA template? - (reply: 1)
      
1342. Long Primers for PCR - (reply: 2)
      
1343. Data Analysis for Real-time PCR - (reply: 2)
      
1344. PCR not working - overhangs too long? (reply: 3)
      
1345. Apoptotic gene expression - Help required in RT-PCR for apoptotic genes (reply: 1)
      
1346. argh more pcr headaches! - (reply: 6)
      
1347. With 40 pcr cycles, how relevant are samples with Ct's of 35-40? - Help me please, I'm going nuts over this problem!! (reply: 2)
      
1348. Chlamydia PCR cycling conditions - (reply: 1)
      
1349. Primers going "loopy" - (reply: 2)
      
1350. Conversion of primer unit - ug/ul to uM (reply: 4)
      
1351. mutagenesis by PCR or just use a kit - (reply: 5)
      
1352. Unsuccessful BS PCR - (reply: 3)
      
1353. Primer Trouble Shooting - (reply: 5)
      
1354. Proven Primers Stopped working & now leave smear! - (reply: 2)
      
1355. Smearing on gel in PCR products - (reply: 5)
      
1356. Ethanol in PCR clean-up - Can I trust lab ethanol stocks (reply: 3)
      
1357. Relative RT-PCR, multiplate and calibration - (reply: 4)
      
1358. Don't understand why we need RT-PCR? - Slight Confusion guys, please clear me up (reply: 6)
      
1359. Relative RT-PCR - (reply: 2)
      
1360. Relative real time RT PCR - (reply: 2)
      
1361. DTT - WHY DTT IS USED IN RT- PCR (reply: 1)
      
1362. 40 pcr cycles and Ct-values of 36-40 - trust or not? - (reply: 1)
      
1363. a simple primer problem - (reply: 5)
      
1364. Failure to introduce mutations using Overlap PCR - (reply: 3)
      
1365. no or poor amplification - (reply: 1)
      
1366. Primers from 3'UTR - (reply: 2)
      
1367. Strange RT-PCR Graph - Is this inhibition of PCR? (reply: 1)
      
1368. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
      
1369. Designing primers with ESTs - (reply: 2)
      
1370. Direct sequencing of Bisulphite PCR product - (reply: 1)
      
1371. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
      
1372. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
      
1373. Real Time vs Traditional PCR results - (reply: 1)
      
1374. The problems to identify mouse genotype with PCR - (reply: 1)
      
1375. addition of BclI restriction site to PCR primer - (reply: 6)
      
1376. non specific amplification - DNA amplification by PCR (reply: 1)
      
1377. Molecular Cloning - Cloning of PAPD PCR products (reply: 1)
      
1378. How does methprimer calculate primer Tm - (reply: 2)
      
1379. Laminar Flow vs PCR cabinet - (reply: 4)
      
1380. How to do PCR to detect mRNA without RT? - (reply: 2)
      
1381. first strand cDNA synthesis not working - cannot PCR out cDNAs of interest from first strand cDNA (reply: 2)
      
1382. Colony PCR with Eukaryotic Cells? - Any Experiences? (reply: 4)
      
1383. PCR has stopped working - (reply: 7)
      
1384. PCR negative control contamination - (reply: 8)
      
1385. LacZ PCR genotyping -ve control contamination - (reply: 3)
      
1386. Phosphorylated primers - (reply: 5)
      
1387. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
      
1388. PCR efficiency in real timePCR - (reply: 5)
      
1389. Amplification from plasmid DNA, but not from genomic DNA with the same target re - (reply: 4)
      
1390. Drastic Decrease in PCR product yield - (reply: 2)
      
1391. resources on PCR principles & technique - (reply: 1)
      
1392. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
      
1393. Colony Screeing without using PCR - (reply: 8)
      
1394. High MW PCR band seen...help needed - (reply: 7)
      
1395. QPCR - Primer and Probe question - QPCR (reply: 2)
      
1396. Urgent Help needed: RNA-Interference, rt-pcr and Western-Blot do not match - (reply: 2)
      
1397. standard curve/ PCR efficiency problems... - (reply: 1)
      
1398. Help! PCR that used to work doesn't work now! - (reply: 2)
      
1399. PCR and templates - (reply: 3)
      
1400. does anyone have experience with pwo DNA polymerase for long PCR fragments ? - (reply: 2)
      
1401. Real Time PCR internal reference (housekeeping) gene in E. coli - is rrsB suitable (reply: 1)
      
1402. PCR problems on high GC content gene - Trouble with Colony PCR of TOP10 transformants w/ TOPO-vector (reply: 4)
      
1403. Easy primer question? - Primers (reply: 1)
      
1404. PCR inconsistency - (reply: 4)
      
1405. Plasmid problem - From PCR product (reply: 7)
      
1406. help needed: PCR a gene from genomic DNA - (reply: 2)
      
1407. questions about pcr products after pooling - (reply: 3)
      
1408. stiching/linking/sewing pcr - (reply: 3)
      
1409. Human mtDNA amplification problems - (reply: 3)
      
1410. primer dimer - proplems (reply: 1)
      
1411. Blasting Primers for RT-PCR - what's hypothetical proteins? My primers always match to these!! (reply: 2)
      
1412. Nested PCR - (reply: 2)
      
1413. pfu vs long pcr mix - (reply: 7)
      
1414. weird PCR ask for help - (reply: 12)
      
1415. Methylation specific DMSO PCR - (reply: 1)
      
1416. The reliable data of microRNA expression from SYBR-stem loop PCR or ABI Taqman m - (reply: 8)
      
1417. No PCR product at all - (reply: 8)
      
1418. PCR troubleshoooooting - primer dimer (reply: 1)
      
1419. Primer efficiency test - (reply: 1)
      
1420. primer software - (reply: 2)
      
1421. Question on wired PCR - (reply: 1)
      
1422. restriction digestion of PCR product - (reply: 7)
      
1423. Primer design and Blast program at NCBI - comparison Primer3 and Primer Blast (reply: 1)
      
1424. Checking PCR insert (into pGEMT vector) - (reply: 2)
      
1425. How to know in which exon a primer match? - (reply: 1)
      
1426. primer annealing - higher annealing= less wrong bounds (reply: 4)
      
1427. Amplification of human genomic DNA - (reply: 2)
      
1428. trouble amplifying 2.5kb product from genomic DNA - (reply: 3)
      
1429. sequencing with forward/reverse primers - (reply: 7)
      
1430. How to choose the parameters (Tm, cycli) for RT-PCR - (reply: 1)
      
1431. PCR troubleshooting - two band after agrose electophoresis (reply: 4)
      
1432. Adding A overhangs - primer design implications? - (reply: 1)
      
1433. primer RE over-hang nucleotides - common sequences? (reply: 5)
      
1434. Forward and reverse primers got very different Tm - what to do? (reply: 11)
      
1435. Colony PCR screen positive - insert digestion negative - (reply: 2)
      
1436. Q-PCR: Strange Amplification Curve shape (non exponential) - (reply: 3)
      
1437. Finding cDNA for making a standard curve for real-time RT-PCR - (reply: 1)
      
1438. primer design problem - (reply: 1)
      
1439. Bisulfite Sequencing PCR help! - BSP is Failing like Gangbusters. Please help! (reply: 6)
      
1440. Problems with GOI Ct's - How to do PCR efficiency test if there is no Ct in GOI? (reply: 7)
      
1441. Problem with primer efficiency analysis - (reply: 4)
      
1442. slan real time PCR system for validation of microarray results? - (reply: 1)
      
1443. PCR unusualband at ~230bps - PCR (reply: 1)
      
1444. DMSO or BSA for PCR - (reply: 6)
      
1445. Whole Genome Amplification - (reply: 2)
      
1446. primer design - (reply: 1)
      
1447. Settings op a RT-PCR, What is my next step - I`ve got RNA --> cDNA and working primers, what now? (reply: 1)
      
1448. Separating PCR product on agarose gel with similar sizes - (reply: 5)
      
1449. designing primers for genes not sequenced yet - (reply: 2)
      
1450. Tris Buffer for PCR reaction - why? (reply: 1)
      
1451. PCR a plasmid protein - (reply: 2)
      
1452. multiplex PCR - (reply: 4)
      
1453. Realtime PCR machine - (reply: 2)
      
1454. PCR set-up calculation nightmares. - (reply: 3)
      
1455. Primer desing - (reply: 2)
      
1456. Colony PCR - (reply: 3)
      
1457. Standard curves for PCR efficiency. - (reply: 2)
      
1458. Increasing the number of products your PCR produces - Degenerative primers for multiple copy gene (reply: 3)
      
1459. Question about RAPD PCR - (reply: 2)
      
1460. Looking for help with my PCR! - (reply: 3)
      
1461. How to incorporate dUTP when using WGA2 amplification for Affymetrix’s array? - (reply: 2)
      
1462. Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq? (reply: 7)
      
1463. primer design - (reply: 9)
      
1464. problem with cloning PCR - can't amply the full-length cDNA with PCR (reply: 6)
      
1465. PCR without thermal cycler? - (reply: 9)
      
1466. primer contamination - primer contaminated with ice (reply: 3)
      
1467. Loss of volume in PCR reaction in 96 well plate - (reply: 7)
      
1468. RT-PCR problem - (reply: 4)
      
1469. Only DNA ladder , No desired band in PCR - (reply: 4)
      
1470. taq and PCR - (reply: 6)
      
1471. Does purifying PCR probes for EMSA from EtBr gel interfere with binding? - (reply: 1)
      
1472. PCR Efficiency - (reply: 2)
      
1473. DNA and RNA contamination in RT PCR water controls - (reply: 2)
      
1474. Long PCR and genomic DNA isolation problems - (reply: 2)
      
1475. Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
      
1476. NTC with specific amplification - (reply: 1)
      
1477. Problem cloning bisulfite PCR BSP product - (reply: 6)
      
1478. Opinions on Fermentas DreamTaq Green PCR Master Mix? - (reply: 3)
      
1479. Primer3 vs Primer BLAST - (reply: 3)
      
1480. PCR and then ligation - (reply: 7)
      
1481. Primer design: free energy - (reply: 2)
      
1482. Amplification in NTC and noRT controls - (reply: 5)
      
1483. realtime PCR interpretation-peak found in negative control but no Ct value - (reply: 6)
      
1484. Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)
      
1485. asymmetric PCR - (reply: 1)
      
1486. PCR amplifying 50bp ssDNA ? - PCR amplification (reply: 6)
      
1487. CORRECT PCR Incorrect RTPCR - (reply: 10)
      
1488. Is there a way to "rescue" an already-completed extraction from PCR in - (reply: 1)
      
1489. trouble with pcr - (reply: 8)
      
1490. Interpreting melting curve data in Sybr Green RT-PCR - (reply: 12)
      
1491. Confusing bands from PCR - not primer dimer, not product (reply: 30)
      
1492. Real time PCR results-interpretation - (reply: 1)
      
1493. Real time PCR results-interpretation - (reply: 2)
      
1494. Primer optimization for ChIP - (reply: 2)
      
1495. sequencing primer: T7 or T7promoter? - (reply: 4)
      
1496. 2-step or 3-step real time PCR - question about real time PCR (reply: 6)
      
1497. Problems with my PCR's - Having an issue with some of my samples (reply: 2)
      
1498. SYBR melting curve vs RT-PCR gel - (reply: 1)
      
1499. primer tm - tm calculation for tagged primers (reply: 2)
      
1500. Reusing 96 well plates for PCR - (reply: 2)
      
1501. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
      
1502. PCR and AFLP - (reply: 1)
      
1503. Universal 16s rRNA primers needed - (reply: 3)
      
1504. Identifying PCR Inhibitors - (reply: 10)
      
1505. PCR primers for histone mod ChIP - How to find the regions (reply: 1)
      
1506. PCR + phusion enzyme = massive errors - (reply: 4)
      
1507. long DNA amplification - (reply: 11)
      
1508. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
      
1509. gDNA pcr product as standard for absolute quantification? - (reply: 1)
      
1510. adding koazak sequence to primer - (reply: 1)
      
1511. PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
      
1512. Designing primers for cloning - After primer designing, how should I perform the PCR? (reply: 15)
      
1513. pcr trouble solved but I cant seem to understand why - (reply: 1)
      
1514. Primer design if sequence is unknown for organism - (reply: 5)
      
1515. adding C terminal tag to reverse primer - (reply: 1)
      
1516. epitope tagging of pcr products - (reply: 1)
      
1517. Is Taq polymerase still active after staying at 10C for one day? - (reply: 2)
      
1518. Real time PCR info - (reply: 4)
      
1519. Flox and Cre Primers - PCR Troubleshooting - Please Help (reply: 2)
      
1520. PCR from BAC - PCR from BAC (reply: 1)
      
1521. Designing Primers for RT-PCR after ChIP - Help to avoid primer dimers (reply: 2)
      
1522. Failed TA cloning with Fusion PCR product! - I need help with TA cloning of an Overlap PCR product (reply: 1)
      
1523. E. coli 16s - looking for specific primer+probe for E. coli (reply: 1)
      
1524. How much product PCR?? - (reply: 3)
      
1525. what primers should I use for DNA sequencing? please help - (reply: 2)
      
1526. what primers should I use for DNA sequencing?? - (reply: 3)
      
1527. Is my primer going to work for qRT-PCR? - (reply: 6)
      
1528. Standard curve for RT-PCR - (reply: 1)
      
1529. PCR using genomic DNA - (reply: 2)
      
1530. pcr trouble!!! heeeeeellllpppppp!! - (reply: 1)
      
1531. Problem amplifying plasmid - (reply: 1)
      
1532. Sybr Green I in real-time PCR reaction - (reply: 3)
      
1533. Primers annealing temperatures - (reply: 8)
      
1534. Difficulties cloning by PCR - (reply: 5)
      
1535. PCR Result Explanation (I needed you help) - Refer to the result, what is indicate A? (reply: 4)
      
1536. Manual reverse primer design for bisulfite traeted DNA - (reply: 4)
      
1537. high Cp value PCR - (reply: 2)
      
1538. real time PCR data presentation - presentation of delta CT and copy number (reply: 2)
      
1539. Synthesize own Oligo-dT primers - (reply: 6)
      
1540. Testing primer specificity... - ...without gDNA of target organisms (reply: 6)
      
1541. shRNA shows knockdown in protein expression but no change in RT-PCR - (reply: 2)
      
1542. dNTP or Deoxynucleotide solution Mix - (reply: 1)
      
1543. Smearing/ no amplification in PCR - (reply: 3)
      
1544. primer design - Primer design for PCR (reply: 1)
      
1545. How are primers designed? - (reply: 4)
      
1546. Source of DNA for a Reporter Construct - How to PCR the promoter region of interest? (reply: 1)
      
1547. DNA methylated PCR - (reply: 2)
      
1548. need help on primer calcuation soon - (reply: 1)
      
1549. primer design - (reply: 6)
      
1550. problem in pcr and purification - (reply: 3)
      
1551. Inability to duplicate PCR results - (reply: 8)
      
1552. Direct PCR (PCR without DNA extraction) - (reply: 2)
      
1553. PCR using xt5 - (reply: 2)
      
1554. long range PCR - purification of pcr frogament 9-13kb (reply: 9)
      
1555. BSP PCR for help - no bands of tissue amplification (reply: 3)
      
1556. Help! How to get rid of a repeatedly appearing smear in my RT-PCR result! - (reply: 2)
      
1557. 2 bands or smear? 2-step RT-PCR - (reply: 1)
      
1558. 23S rRNA Primer - need primer sequence (reply: 1)
      
1559. Methylation specific PCR - Methylation specific PCR - problem with controls.... (reply: 2)
      
1560. RT-PCR - problems with TaqMan PCR (reply: 2)
      
1561. Problems with Phusion Polymerase (Finnzymes) - (reply: 3)
      
1562. Multiplex PCR - Multiplex PCR and gel electrophoresis (reply: 1)
      
1563. template for PCR for cloning purposes, miniprep or maxiprep? - (reply: 3)
      
1564. Homogenizing animal tissue to RT-PCR in real-time - (reply: 2)
      
1565. Small product amplification problems - (reply: 4)
      
1566. multi way ligation or long range pcr? - (reply: 1)
      
1567. Freezing primers+SYBR green - (reply: 2)
      
1568. Tagged Primers - (reply: 5)
      
1569. no pcr amplification product - (reply: 9)
      
1570. Primer Dimer in NTC Only? - Primers Dimers show up in NTC but not template wells (reply: 4)
      
1571. design primer for chip - (reply: 5)
      
1572. How to quantify oligonucleotide primers using nanodrop ND-1000 software - newbie help (reply: 2)
      
1573. PCR problem - (reply: 4)
      
1574. differently behavioring replicates in real-time PCR - (reply: 1)
      
1575. Multiplex PCR - (reply: 1)
      
1576. Is necessary to use pfu polymearse for PCR amplification of pre-miRNA for clonin - (reply: 4)
      
1577. No amplification in PCR using gDNA extracted from formailin fixed paraffin embed - (reply: 3)
      
1578. Requirement of HPLC/PAGE purified primers in Quikchange mutagenesis - (reply: 3)
      
1579. Volume of DNA to add to PCR Master Mix? - (reply: 1)
      
1580. how much DNA you need for real time pcr after chip - CHIP (reply: 1)
      
1581. PCR components storage - (reply: 3)
      
1582. reconstitution of primers - (reply: 2)
      
1583. No PCR bands with some templates - (reply: 5)
      
1584. Mysterious PCR Contamination - (reply: 13)
      
1585. BS PCR..please help - DNA methylation analysis (reply: 15)
      
1586. RT-PCR Help - Protocol provided (reply: 4)
      
1587. PCR product purification - (reply: 3)
      
1588. Mutagenesis PCR and undesired amplification - (reply: 2)
      
1589. Temp Gradient PCR - (reply: 2)
      
1590. Vector sequence included in (PCR) amplified insert - (reply: 2)
      
1591. PCR with genomic dna - (reply: 9)
      
1592. primer dimer - (reply: 2)
      
1593. PCR with long primers - (reply: 3)
      
1594. BSP PCR standardization...help - (reply: 8)
      
1595. PCR on degraded templates - (reply: 2)
      
1596. PCR help needed - (reply: 3)
      
1597. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
      
1598. Inversed PCR issues - DNA isolation, restriction, ligation and PCR with no success (reply: 1)
      
1599. light bands, dark smear, dark dimers - (reply: 2)
      
1600. PCR, followed by sequencing... - why ?? (reply: 3)
      
1601. PCR program - strange extension step (reply: 2)
      
1602. Purifying dsDNA from ssDNA after a PCR reaction - (reply: 3)
      
1603. Help me! How to design nested BSP primers? - (reply: 4)
      
1604. REAL TIME PCR 7005 v 2.01 : I need more reference samples! - How to add more than one reference sample to Real Time?? (reply: 1)
      
1605. Plasmid linearization by PCR - (reply: 4)
      
1606. In-fusion advantage PCR cloning kit - --- failure after many many months-- (reply: 13)
      
1607. Using BLAST to check primers - (reply: 17)
      
1608. ExoSap then nanodrop? - Quantifying PCR products after exosaping! (reply: 2)
      
1609. pcr doesnt show any band for DNa walking - (reply: 8)
      
1610. storing large amounts of PCR mastermix beforehand - question (reply: 3)
      
1611. questions about semi-quantitive PCR - weird result form semi-quantitive PCR (reply: 2)
      
1612. Effects of more cDNA in PCR??? - (reply: 1)
      
1613. PCR primer - (reply: 2)
      
1614. PCR product disappears after restriction digestion - (reply: 4)
      
1615. Primer design - GC clamp - (reply: 8)
      
1616. Real Time PCR - excluding Ct - (reply: 2)
      
1617. PCR product looks less intense compared to others - should I increase the number of cycles? (reply: 4)
      
1618. Price of a real time PCR machine? - (reply: 4)
      
1619. colony PCR - (reply: 8)
      
1620. probe conc. of Asymmetric PCR - (reply: 1)
      
1621. primer dimer or what? - (reply: 2)
      
1622. PCR stopped working!!! HELP!!! - need results urgently (reply: 12)
      
1623. Real Time PCR method comments - (reply: 2)
      
1624. Sybre green taq man PCR assay for quantitative methylation detection - (reply: 2)
      
1625. colony pcr - (reply: 4)
      
1626. Primers and Taqman Probes mixture question - (reply: 1)
      
1627. truble shooting qiagen 1 step rt pcr kit - (reply: 2)
      
1628. ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)
      
1629. Not my insert size after colony PCR! - After colony PCR, the 'insert' size is 1kb more! (reply: 1)
      
1630. Colony PCR - (reply: 15)
      
1631. Trouble with PCR on genomic tomato DNA (I've tried many fixes) - (reply: 5)
      
1632. no bands in my PCR - (reply: 9)
      
1633. designing primers - (reply: 1)
      
1634. Real Time PCR issues - Problems regarding my qPCR (reply: 6)
      
1635. purefication and amplification of serum free DNA - (reply: 1)
      
1636. How to use Real-time PCR to detect some gene copy numbe in plant genome? - (reply: 1)
      
1637. Bisulfite Sequencing PCR - (reply: 4)
      
1638. Restriction Enzyme Digest of Genomic DNA - Problem with RE-digest + PCR in CpG island assay (reply: 1)
      
1639. way to know if my UNOII pcr machine is working properlly - (reply: 3)
      
1640. Freezing DNA have effects on real-time PCR efficiencies - (reply: 1)
      
1641. mRNA PCR - (reply: 1)
      
1642. pcr product quantification - (reply: 5)
      
1643. Negative Control Primers for ChIP Assay - (reply: 3)
      
1644. Magnesium in PCR - (reply: 1)
      
1645. PCR Sample Prep - (reply: 4)
      
1646. Bisulfite PCR Inconsistent, often smears - (reply: 4)
      
1647. PCR from roots - I know this sounds a little crazy... (reply: 2)
      
1648. Bizarre Contamination in PCR - (reply: 7)
      
1649. PCR Cloning-large primers - (reply: 4)
      
1650. Quantifying ingested bacteria without realtime PCR - (reply: 3)
      
1651. forward and reverse primer - (reply: 4)
      
1652. Check the primers - (reply: 4)
      
1653. PCR problem from transformed TOPO TA vector - (reply: 3)
      
1654. DIG DNA PCR labeling problem - (reply: 6)
      
1655. PCR product stays on agarose gel well !! - I need help, what is it happening? (reply: 11)
      
1656. BSP primers - (reply: 2)
      
1657. Various size of insert after colony screen by PCR - (reply: 2)
      
1658. inverse PCR molecular bilogy - (reply: 3)
      
1659. PCR data analysis if the efficiencies aren't equal - real time PCR troubleshooting (reply: 2)
      
1660. failed PCR on DNA extract from blood - (reply: 6)
      
1661. Will ligase buffer affect polymerase fidelity? - (reply: 2)
      
1662. Primer dimer issue in real time PCR - (reply: 21)
      
1663. Leaky RT-PCR - (reply: 4)
      
1664. Preparing PCR reactions from a master mix - Just a quick one (reply: 3)
      
1665. PCR, RNA, Northern Blotting???? - (reply: 1)
      
1666. bizzare PCR smear - why does science hate me (reply: 7)
      
1667. RT-PCR primer dimers and cDNA degradation - (reply: 2)
      
1668. PCR detection of SNP - (reply: 6)
      
1669. PCR efficiency important in real time absolute quantification? - (reply: 4)
      
1670. PCR contamination with human DNA first and now no bands!!! - (reply: 4)
      
1671. methylation erased by pcr: how and why - why does PCR erase methylation information? (reply: 7)
      
1672. Sub-cloning sticky-end PCR products - (reply: 2)
      
1673. sequencing result for primer designing - sequnce hit primer or gene? (reply: 1)
      
1674. ChIP on chip amplification problems - (reply: 11)
      
1675. Genotyping PCR - (reply: 3)
      
1676. PCR screening of transformed bacterial colony - (reply: 6)
      
1677. Is this standard valid? - pcr product as standards (reply: 5)
      
1678. primer binding - (reply: 1)
      
1679. weird ChIP primers! - (reply: 1)
      
1680. PCR primers - (reply: 1)
      
1681. Unwanted 100bp in RT-PCR - 100bp band appearing all the time after RT-PCR (reply: 7)
      
1682. Slippage of PCR polymerame - How do I avoid it ? (reply: 6)
      
1683. amplification in negative control in RT PCR - (reply: 2)
      
1684. miRNA real time PCR by SYBR green methods - (reply: 1)
      
1685. Deletion PCR - (reply: 9)
      
1686. diluting to final primer concentration - please help correct (reply: 1)
      
1687. Smear problem with my new primer set - (reply: 2)
      
1688. PCR ghost bands - (reply: 1)
      
1689. Amplifying a gene using a degenerate primer - (reply: 4)
      
1690. DNA extraction and PCR advice requested - PCR, Contamination, Low abundance targets (reply: 7)
      
1691. CP of real time PCR by LC480 - (reply: 1)
      
1692. CHIP- Real Time PCR calculations - (reply: 1)
      
1693. volume of DNA required (PCr product ) in agarose gel - (reply: 11)
      
1694. transgene copy number - using real time PCR (reply: 1)
      
1695. real-time PCR and HIV - what is the most commonly used assay to measure viral load in plasma (reply: 2)
      
1696. Making Primer Dimers on Purpose - odd I know...but humor me (reply: 5)
      
1697. Unspecific PCR - (reply: 7)
      
1698. Real-Time PCR as a Microplate Reader - (reply: 1)
      
1699. gene-specific RT-PCR - (reply: 4)
      
1700. Primer Design - Design Primer with DNA Sequence (reply: 1)
      
1701. RT-PCR Internal Standard - (reply: 2)
      
1702. PCR arrays=Primers in a plate? - Can any PCR mix be used? (reply: 1)
      
1703. Plasmid supercoiling affecting PCR? - (reply: 2)
      
1704. RNA contamination in PCR - (reply: 1)
      
1705. PCR without DNA extraction! - (reply: 2)
      
1706. Primer tm - (reply: 1)
      
1707. How to Clone a 2.3 Kb Gene with PCR - (reply: 2)
      
1708. re-amplification in real-time PCR - (reply: 1)
      
1709. primer design - really necessary? (reply: 2)
      
1710. TaqMan real time PCR using abi 7700 system-troubleshooting - (reply: 5)
      
1711. RT-PCR - (reply: 3)
      
1712. Long product amplification (1598bp) - PCR for a product of 1598bp length (reply: 3)
      
1713. Degenerative primers = multiple products? - (reply: 5)
      
1714. Quantify RNAi(siRNA) efficacy - Designing primers for quantification (reply: 2)
      
1715. PCR problems on a ligation product - (reply: 5)
      
1716. Genomic DNA and PCR - (reply: 4)
      
1717. Primer dimers - shouldn't they also occur in the NTC? (reply: 5)
      
1718. Sewing PCR - (reply: 3)
      
1719. on the minimal pcr derived product - (reply: 2)
      
1720. primer dimers - (reply: 5)
      
1721. RT-PCR vs. conventional PCR - (reply: 1)
      
1722. qPCR primer design - (reply: 5)
      
1723. PCR and gel purification problem - did I screw this up (reply: 4)
      
1724. Cloning PCR fragment - (reply: 1)
      
1725. touchdown pcr - (reply: 6)
      
1726. non specific pcr products - (reply: 4)
      
1727. RT-PCR primer design - Intron/exon boundaries - (reply: 6)
      
1728. Anyone with experiences in 2 step PCR - (reply: 1)
      
1729. PCR works on lab strain but not patient sample - HIV related (reply: 4)
      
1730. confirming concentration for primers in rt-pcr - RT-PCR, Primers (reply: 2)
      
1731. DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
      
1732. Nucleic acid staining and PCR - Query onthe effect of nucleic acid stains on PCR (reply: 1)
      
1733. re-amplification of a PCR product - is it recommended? (reply: 7)
      
1734. mouse Gapdh chip-qPCR primers for control! - (reply: 7)
      
1735. PCR machine with accurate temperature control? - (reply: 1)
      
1736. Gene-specific RT-PCR - (reply: 2)
      
1737. Primer stock confusion - (reply: 6)
      
1738. VWR Real-Time PCR plates for eppendorf Realplex - (reply: 2)
      
1739. primer design - primer design question (reply: 2)
      
1740. Protocol for 16S rDNA real time PCR - (reply: 2)
      
1741. Touchdown PCR - (reply: 1)
      
1742. Determine annealing temperature of primers - (reply: 14)
      
1743. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
      
1744. polymerase for BSP - (reply: 2)
      
1745. real time PCR primer designing problems,again - is NCBI's primer blast believable enough？ (reply: 3)
      
1746. Validating real time pcr primers - (reply: 2)
      
1747. BSP,MSP primer design? - (reply: 1)
      
1748. low gc primers - help with pcr using low gc primers (reply: 6)
      
1749. Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)
      
1750. Primers and annealing temperature - (reply: 2)
      
1751. Good RT-PCR amplication but NO amplification with semi-quantitative PCR!? Wh - (reply: 3)
      
1752. Copy number calculations in real time PCR - (reply: 2)
      
1753. 4 degree hold in PCR machine - Does this hurt the machine? (reply: 3)
      
1754. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
      
1755. RT-PCR Negative controls - (reply: 1)
      
1756. WHich primer to use for sequencing - (reply: 5)
      
1757. New Technologies Real time PCR, cloning, microarray, sequencing - (reply: 3)
      
1758. minimum length for the gene to be amplified in PCR - (reply: 6)
      
1759. dNTP Quantity - (reply: 3)
      
1760. annoying PCR cloning problem (season 2) - (reply: 5)
      
1761. PCR with 60 bp primers - results in no product (reply: 6)
      
1762. Amplification dwindles using little template RT-PCR - (reply: 3)
      
1763. Running gel after RT-PCR - (reply: 5)
      
1764. Degenerate PCR Size Limit? - (reply: 3)
      
1765. primer design for pBAD topo expression kit - (reply: 2)
      
1766. PCR problems.. - problems with conventional PCR (reply: 3)
      
1767. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
      
1768. gDNA or cDNA amplification in RT-PCR - (reply: 3)
      
1769. small PCR products on agarose gel? - (reply: 8)
      
1770. Dnase digestion and PCR - (reply: 4)
      
1771. Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)
      
1772. primers fpr sequencing - (reply: 4)
      
1773. MSP: No PCR product for one cell line but yes for the other cell lines - (reply: 3)
      
1774. Need an UV260 RT-PCR instrument - (reply: 1)
      
1775. Nested Relative Quatitative Real-Time RT-PCR - (reply: 5)
      
1776. Any mRNA amplification kit for microarray? - I have very little amount of RNA but have to submit it for microarray. (reply: 4)
      
1777. PCR produces products of wrong size - (reply: 4)
      
1778. PCR with pfu and degenerate primers (?) - (reply: 7)
      
1779. a doubt about PCR gel purification - (reply: 4)
      
1780. Draw PCR primer locations - PCR primer (reply: 4)
      
1781. how to design PCR site-directed multiple mutagenesis - (reply: 1)
      
1782. RT-PCR primers on coding sequence or UTRs? - (reply: 4)
      
1783. Program for mapping primers to gene - diagram maker, visualize - (reply: 4)
      
1784. Using Real Time PCR for Cell Viability - Any tips? (reply: 6)
      
1785. PCR+enhancer - (reply: 4)
      
1786. Serious issues with RT-PCR - (reply: 7)
      
1787. PCR [dNTP] - dNTP concentrations (reply: 2)
      
1788. PCR issue - (reply: 2)
      
1789. dNTP question - (reply: 3)
      
1790. PCR - (reply: 1)
      
1791. long PCR primer - (reply: 7)
      
1792. Taqman rtPCR primer and probe design - (reply: 3)
      
1793. sequencing problem after pcr - (reply: 4)
      
1794. Amplifying plasmid - non specific binding of primer - (reply: 3)
      
1795. Help with Real Time PCR Well To Well Variation - (reply: 10)
      
1796. primer design - (reply: 3)
      
1797. Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (old and useful thread) (reply: 2)
      
1798. Problems digesting PCR product - troublesome enzymes: NotI PvuI (reply: 2)
      
1799. Analysis of ChIP RT-PCR data - (reply: 7)
      
1800. Make construct so it is only thing that can PCR after transfection - (reply: 2)
      
1801. Negative flouroscence in real time PCR - (reply: 2)
      
1802. Minimum number of PCR cycles to see a product? - (reply: 6)
      
1803. decontamination - decontamination for PCR (reply: 6)
      
1804. Troubleshoot ARMS PCR - (reply: 2)
      
1805. negative control for PCR - (reply: 1)
      
1806. how to design the primers for Real-Time PCR?? - I can't find the proper ones... (reply: 7)
      
1807. design primer - when I design a primer is it necessary to include the restriction (reply: 1)
      
1808. pcr pdt amplification - (reply: 1)
      
1809. real time PCR - (reply: 3)
      
1810. PCR fails when I scale up - (reply: 2)
      
1811. can we use one-step RT-PCR kits to amplify DNA? - (reply: 1)
      
1812. PCR bands in NTC Control and Neg Control Lanes - (reply: 4)
      
1813. PCR not Working = SMEAR - (reply: 3)
      
1814. How to do PCR fragment in 2 steps - (reply: 3)
      
1815. Difference in Primer Melting Temperatures - Maximum allowable Tm difference? (reply: 4)
      
1816. where to buy rotorgene real-time rt-pcr kits? - (reply: 4)
      
1817. Real-Time RT-PCR one-step and two step issue - (reply: 13)
      
1818. RT-PCR - problem of PCR (reply: 1)
      
1819. PCR product longer than template - why? - (reply: 2)
      
1820. Problem with Colony PCR - Problem with gel for colony PCR (reply: 3)
      
1821. Dye recipe that can be used for PCR MM - (reply: 3)
      
1822. Primers Not working - Real time PCR using SYBR Green (reply: 3)
      
1823. PCR conditions with three primers - (reply: 1)
      
1824. Real-Time PCR primer vs conventional PCR primer - (reply: 5)
      
1825. distance between probe and primers in Taqman method - how far should they be? (reply: 2)
      
1826. Cloning a labeled PCR product - (reply: 1)
      
1827. PCR and cloning - (reply: 14)
      
1828. QuantiTect Primers with Roche SYBR - ....can you mix and match? (reply: 2)
      
1829. PCR product - not suppose to be there (reply: 7)
      
1830. using the meth primer - (reply: 2)
      
1831. Generating primers for MSP - (reply: 1)
      
1832. Digest genomic (eukaryote) DNA before running PCR, is it necessary? - I do not get any band from PCR using genomic DNA and different primers (reply: 6)
      
1833. real time RT-PCR, 1-step vs. 2-step method - (reply: 2)
      
1834. PCR from a smear genomic DNA ? - (reply: 5)
      
1835. Primer design and need help - (reply: 11)
      
1836. Setting up a multiplex PCR assay. - (reply: 2)
      
1837. Programme for degenerate primer design - Programme for primer design (reply: 9)
      
1838. Negative Control for ChIP realtime PCR in Mouse - (reply: 12)
      
1839. cDNA and RT-PCR - (reply: 8)
      
1840. Cloning purified PCR products eluted in Qiagen's EB - Concentrate a purified PCR product eluted with EB? (reply: 11)
      
1841. Proofreading polymerase problem - Anyone experienced similar problems? (reply: 5)
      
1842. PCR problem - help me plsss (reply: 9)
      
1843. gene-specific qPCR primers for a multigene family - primer design (reply: 5)
      
1844. Important literature for real time PCR users - Important documents for beginners as well as advanced users (reply: 41)
      
1845. PCR efficiencies - (reply: 4)
      
1846. RT-PCR primer design guide - How to check gene structure and design the primer? - Recovered post (reply: 4)
      
1847. PCR quantification - (reply: 1)
      
1848. PCR quantification - (reply: 1)
      
1849. Long PCR product - (reply: 9)
      
1850. problem in Pcr amplification - (reply: 7)
      
1851. What does 'limiting dilution PCR' mean? - I wanna know the meaning of 'limiting dilution' (reply: 4)
      
1852. difference between hotstart and taq polymerase - (reply: 7)
      
1853. sequencing the pcr product - (reply: 15)
      
1854. Pcr amplification - primer designed from UTR regions (reply: 5)
      
1855. RT-PCR - can you keep the PCR plate in the fridge? - Please help! (reply: 1)
      

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